Figure 2From: GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field useEstimation of genomic DNA quantity and quality. Bar graph represents average estimated DNA concentrations from at least 50 extracted samples (A; ng/μl), as defined by gel density analysis (white bars), or spectrophotometric determinations using a NanoDrop spectrophotometer (blue bars). Results were obtained for DNA samples extracted using Promega's Genome Wizard kit (I), the Nucleon Phytopue Kit (II) or the CTAB-homebrew method (III). Collated absorbance ratios obtained from the same samples at 260: 280 nm (green bars), or 260: 230 nm (grey bars) are shown. DNA quantity and integrity was assessed by resolving DNA samples (extracted from Bt11 maize reference material; 0%, - 5%) on 0.8% TAE agarose gels by electrophoresis. Representative samples extracted using Promega's Genome Wizard kit (B), the Nucleon Phytopue Kit (C) or the CTAB-homebrew method (D).Back to article page