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Table 2 Half-life of laccase measured by ABTS oxidation.

From: Bacillus pumiluslaccase: a heat stable enzyme with a wide substrate spectrum

 

4°C

25°C

45°C

65°C

McIllvaine buffer pH 7

1.0

0.75

0.32

0.28

Potassium phosphate buffer pH 7

0.93

0.79

0.45

0.34

Deionized water

144

40.0

19.3

3.5

  1. The purified protein was stored in different solvents and at various temperatures. Half-lives are given in hours.