Table 1 Effect of preparation conditions on lipase immobilization efficiency and activity of lipase immobilized PD-MNP
Dopamine (mg/ml) | PD-MNP (mg) | Lipase (mg) | Bound Lipase (mg) | Activity (U) | Specific Activity (U/mg lipase) |
|---|---|---|---|---|---|
0 | 10 | 10 | 3.16 ± 0.52 | 9.75 ± 2.28 | 3.09 ± 0.16 |
0.25 | 10 | 10 | 6.96 ± 0.49 | 18.19 ± 2.90 | 2.62 ± 0.13 |
0.5 | 10 | 10 | 7.81 ± 0.62 | 21.08 ± 2.49 | 2.70 ± 0.17 |
1.0 | 10 | 10 | 8.26 ± 0.48 | 28.81 ± 3.08 | 3.49 ± 0.17 |
2.5 | 10 | 10 | 9.17 ± 0.58 | 59.00 ± 3.39 | 6.44 ± 0.38 |
3.75 | 10 | 10 | 9.37 ± 0.55 | 62.50 ± 3.04 | 6.67 ± 0.37 |
5.0 | 10 | 10 | 9.46 ± 0.57 | 64.89 ± 2.69 | 6.86 ± 0.40 |
2.5 | 10 | 5 | 4.29 ± 0.22 | 37.65 ± 2.52 | 8.78 ± 0.33 |
Free lipase |  | 10 |  | 118.76 ± 4.13 | 11.88 ± 0.41 |