Figure 1

Cell-free protein expression and characterization. A) Cell free GFP and Trx expression and purification by TALON™ Dynabeads^® was assessed by SDS-PAGE and Coomassie Blue staining. U, unpurified total protein extract. P, protein purified with TALON™ Dynabeads^®. B) Western blot analysis of the proteins by using peroxidase-labeled anti-His. U, unpurified total protein extract. P, Proteins purified with TALON™ Dynabeads^®. C) ELISA characterization of the polyclonal phages after different rounds of biopanning. A peroxidase-labeled anti-M13 was followed by TMB incubation to develop the signal. His-tagged BSA and GST were used as negative controls.