From: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer
Sample | Colonies mutant/total | Template doublinga | Mutation frequencyb | Error rate (× 10-6)c |
---|---|---|---|---|
Taq in mix I | 362/5789 | 12.5 | 0.063 | 38.8 |
Taq in mix II | 990/16342 | 10.9 | 0.061 | 43.0 |
Taq in mix III | 2760/21433 | 12.4 | 0.129 | 80.0 |
Taq in mix IV | 951/8273 | 11.7 | 0.115 | 75.6 |
KOD in mix Kd | 30/25006 | 12.1 | 0.0012 | 0.76 |
Various DNA dyes in PCR mix II | ||||
SYTO-9 | 318/4845 | 9.1 | 0.066 | 46.0 |
SYTO-13 | 640/10087 | 9.2 | 0.063 | 44.7 |
EvaGreen | 307/5607 | 9.2 | 0.054 | 38.6 |
LCGreen | 211/4068 | 9.0 | 0.052 | 35.8 |
ResoLight | 64/1243 | 9.1 | 0.052 | 36.2 |