From: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer
PCR mixes* | ||||
---|---|---|---|---|
Component** | I | II | III | IV |
Tris-HCl (mM) [pH]*** | 10 [8.0] | 75 [8.8] | 10 [ 8.0] | 20 [8.8] |
KCl (mM) | 50 | - | 50 | 10 |
(NH4)2SO4 (mM) | - | 20 | - | 10 |
MgSO4 (mM) | - | - | - | 2 |
Triton X-100 (%) | 0.1 | - | 0.1 | 0.1 |
Tween 20 (%) | - | 0.01 | - | - |
MgCl2 mM | 2.5 | 2.5 | 2.5 | - |
DMSO (%) | - | - | 5 | - |
dNTPs μM | 200 | 200 | 200 | 200 |
Taq DNA pol. (U/ml) | 25 | 25 | 25 | 25 |
anti-Taq mAb (nM) | 22 | 22 | 22 | 22 |