Figure 3
From: A suicide gene approach using the human pro-apoptotic protein tBid inhibits HIV-1 replication
Co-transfection of pLtBid(INS) 2 R and pNL4-3/GFP leads to rapid induction of cell death before infectious viruses are produced. Data represent the means and standard deviations of three independent experiments. p-values <0.001 indicate the highly significant impact of tBid on the inhibition of virus production and the induction of cell death. (A) HeLa SS6 cells were transfected with pNL4-3/GFP alone or co-transfected with pNL4-3/GFP and either pLRed(INS)2R or pLtBid(INS)2R. 24 hours after transfection, viral gene expression was determined by analyzing GFP expression using FACS. (B) HeLa SS6 cells were transfected with pLRed(INS)2R (open triangles) or pLtBid(INS)2R (black circles) together with pNL4-3/GFP or transfected with pNL4-3/GFP alone (open circles). Release of viral particles was monitored daily for 4 days by p24 antigen ELISA. (C) Determination of cell death in HeLa SS6 cells transfected only with pNL4-3/GFP or co-transfected with pLRed(INS)2R and pNL4-3/GFP or with pLtBid(INS)2R and pNL4-3/GFP, 24 hours after transfection. Percentages of DCD-stained cells are depicted.