Figure 2
From: A suicide gene approach using the human pro-apoptotic protein tBid inhibits HIV-1 replication
Induction of cell death by tBid expressed by the suicide vector pLtBid(INS) 2 R and its dependency on the presence of HIV-1 Tat and Rev. Shown are representative examples of three independent experiments. (A) HeLa SS6 cells were transfected with the control vector pLRed(INS)2R alone or in addition with pCMV-rev and/or pCMV-tat. 24 hours after infection, expression of dsRed was measured by FACS analysis. Percentages of dsRed-positive cells are given. (B/C) HeLa SS6 cells were transfected with pLRed(INS)2R or the suicide vector pLtBid(INS)2R alone or in addition with pCMV-rev and/or pCMV-tat. 24 hours after infection, cell death was determined by light microscopy (B) and PI-staining followed by FACS analysis (C). Percentages of dead cells are given for cells transfected with pLtBid(INS)2R and in parenthesis for cells tranfected with pLRed(INS)2R. Data obtained by transfection with the control vector pLRed(INS)2R are depicted in white, the results of transfections with the suicide vector pLtBid(INS)2R are shown in grey.