Figure 5

Manipulation of flowering time. TG1 Alc::FTa1 T3 transformants of different ages growing in LD or SD conditions were exposed to ethanol vapour. The time to flowering measured in days after the start of the ethanol (ETOH) treatment was recorded for each plant. n = 12 for each treatment unless otherwise specified. Plants were grown in LD (a-d) or SD (e). a) Distribution of the time to flowering of 10-day-old plants exposed to ETOH for 48 h. One plant had not yet flowered 30 days after the onset of ETOH treatment. Three out of 12 untreated TG1 plants had flowered by this time (the first visible flower buds were observed 39 days after sowing). b) Distribution of the time to flowering of 14-day-old plants exposed to ETOH for 48 h. c) Distribution of the time to flowering of 17-day-old plants exposed to ETOH for 48 h. d) Distribution of the time to flowering of 10-day-old plants exposed to ETOH for 48 h, followed by a further 24 h treatment when the plants were 17 days old. e) Distribution of the time to flowering of 20-day-old plants in SD conditions exposed to ETOH for 48 h, followed by a further 24 h treatment when the plants were 27 days old. n = 24. Seven plants had not yet flowered 30 days after the onset of ETOH treatment. Floral buds were first observed on 4/24 untreated TG1 plants at this time (50 days after sowing).