Figure 5

Ethylene glycol uptake of DrAqp3b-WT and mutants at different pH. Uptake of radiolabeled ethylene glycol by oocytes injected with water or with 5 ng of DrAqp3b-WT, -T85A or -H53A/G54H/T85A cRNA was determined as in Figure 2. Data (mean ± SEM; n = 8-10 oocytes) with an asterisk are significantly (ANOVA, p < 0.01) different from the DrAqp3b-WT.