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Table 2 Short term vapor storage of vitrified embryos on different carriers to simulate transport conditions

From: Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport

 

Cleavage Stage Vitrification

Carrier

Cryoloop

(n = 40)

HSV Straw

(n = 35)

Cryotip

(n = 40)

Storage Condition

LN2

Vapor

LN2

Vapor

LN2

Vapor

Survival (%)

100

100

100

100

100

100

Development to blastocyst after 48 hours (%)

100

100

100

93

100

100

Total blastomeres a (mean ± SD)

82.21 ± 13.28

89.18 ± 18.52

87.20 ± 10.67

88.5 ± 9.55

81.10 ± 14.09

75.53 ± 17.62

 

Blastocyst Stage Vitrification

Carrier

Cryoloop

(n = 41)

HSV straw

(n = 40)

Cryotip

(n = 35)

Storage Condition

LN2

Vapor

LN2

Vapor

LN2

Vapor

Survival (%)

100

100

100

100

100

100

Re-expansion (%)

90

81

95

85

80

85

Total blastomeres a (mean ± SD)

108 ± 18

96 ± 19

96 ± 20

90 ± 20

96 ± 4

86 ± 19

  1. a Total cell count at termination of experiment for both Vit-CL and Vit-BL on day 5
  2. No significant difference in survival, development, re-expansion or cell number after short term vapor storage as compared to liquid nitrogen