Figure 3From: Utilizing high-throughput experimentation to enhance specific productivity of an E.coliT7 expression system by phosphate limitationInfluence of phosphate limitation on respiration activity during cultivation. The oxygen transfer rate (OTR) was measured with the RAMOS device. In panel (A), the mean (n = 2) and standard deviations of duplicate cultivations with 3000 mg/L K2HPO4 (•, blue), 150 mg/L K2HPO4 (■, red) and 75 mg/L K2HPO4 (▲, green) are depicted. In separate shake flasks, samples were collected and analysed for glucose, acetate and phosphate (PO43-). The results of these analyses and the OTR are depicted in (B) for 3000 mg/L, in (C) for 150 mg/L and in (D) for 75 mg/L K2HPO4. The cultivation conditions were as follows: 37°C, shaking diameter 50 mm, shaking frequency 350 rpm, filling volume 10 mL, no induction; modified Wilms & Reuss medium (7.5 g/L Glucose) with various phosphate contents.Back to article page