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Table 4 Analysis of variance (ANOVA) for the experimental results of the CCRD

From: Optimization of culture conditions to produce high yields of active Acetobactersp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones

Source

Biomass (g/L)

Specific activity (μmol/min/g)

 

SS

df

SM

F-value

P-value*

SS

df

SM

F-value

P-value*

Model

0.22

14

0.015

73.18

< 0.0001

256.26

14

18.30

101.9

< 0.0001

X1

0.081

1

0.081

384.8

< 0.0001

25.74

1

25.74

143.3

< 0.0001

X2

0.0024

1

0.0024

11.30

0.0043

20.99

1

20.99

116.8

< 0.0001

X3

0.024

1

0.024

112.7

< 0.0001

0.11

1

0.11

0.6066

0.4482

X4

0.065

1

0.065

307.5

< 0.0001

120.26

1

120.26

669.5

< 0.0001

X1X2

0.0079

1

0.0079

37.38

< 0.0001

3.11

1

3.11

17.33

0.0008

X1X3

0.0062

1

0.0062

29.43

< 0.0001

8.40

1

8.40

46.80

< 0.0001

X1X4

0.00045

1

0.00045

2.143

0.1638

0.28

1

0.28

1.539

0.2338

X2X3

0.00056

1

0.00056

2.677

0.1226

1.08

1

1.08

6.031

0.0267

X2X4

0.00004

1

0.00004

0.1850

0.6729

0.43

1

0.43

2.383

0.1435

X3X4

0.00066

1

0.00066

3.147

0.0964

27.03

1

27.03

150.5

< 0.0001

X12

0.019

1

0.019

91.35

< 0.0001

22.26

1

22.26

123.9

< 0.0001

X22

0.0068

1

0.0068

32.25

< 0.0001

2.18

1

2.18

12.15

0.0033

X32

0.0088

1

0.0088

41.55

< 0.0001

33.01

1

33.01

183.8

< 0.0001

X42

0.0027

1

0.0027

12.67

0.0029

0.68

1

0.66

3.648

0.0755

Lack of fit

0.0025

10

0.00025

1.89

0.2497

0.88

10

0.088

0.24

0.9722

R2 = 0.9856

R2 = 0.9896

  1. X1, glucose; X2, soy peptone; X3, MnSO4·H2O; X4, initial pH.
  2. *P < 0.05 are significant.
  3. SS: Sum of squares; DF: degrees of freedom; SM: mean squares.