Figure 1From: Efficient production of chimeric Human papillomavirus 16 L1 protein bearing the M2e influenza epitope in Nicotiana benthamiana plantsSchematic representation of the synthetic L1ΔC22 gene and of the expressing cassettes made in the pEAQ-HT vector. (a) Synthetic L1ΔC22 gene. Unique restriction sites with nucleotide positions in brackets are shown. Structural helices and β-sheets are indicated as cylinders or white boxes, respectively. The h4 or the coil region connecting h4 and β-J where the substitutions with the M2e epitopes were made are shown in gray or black, respectively. (b) pEAQ-HT expression cassette containing the L1ΔC22 gene, under the control of the Cauliflower mosaic virus 35S promoter (CaMV-P), 5' and 3' non-translated regions (NTR) of Cowpea mosaic virus RNA-2, and nopaline synthase terminator (Nos-T). RB and LB: right and left borders of T-DNA, respectively; P19: suppressor of gene silencing; CaMV-T: CaMV terminator; NPT: neomycin phosphotransferase.Back to article page