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Table 2 Cq values measured by the RT-qPCR assay.

From: A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues

 

Cq≤ 32

32.1 ≤ Cq≤ 35.0

35.1 ≤ Cq≤ 39.9

P1-08

5

14

11

P1-03

24

6

0

P1-98

7

22

1

P1 (total)

36

42

12

P2-08

4

12

14

P2-03

20

10

0

P2-98

5

21

4

P2 (total)

29

43

18

  1. Samples from years 2008, 2003 and 1998 were analysed by each of the two protocols. Each sample of protocol 1 (P1) and 2 (P2) was separated into three groups (based on the Cq value measured for each sample replicate).