Figure 3
From: Sequencing bias: comparison of different protocols of MicroRNA library construction
End Secondary Structure (ESS) of miR-1 sequences in Dorper sheep and Hu sheep data. Reads mapping to miR-1 sequence from Illumina GA data, Illumina V1 cloning, Illumina V1.5 cloning, and SOLiD cloning data of Dorper sheep (A) and Hu sheep (B) were assessed by RNA mfold software. The ratio of reads containing secondary structure at 5'- or/and 3'-end, or containing secondary structure within the sequence (non-ESS) were shown.