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Figure 5 | BMC Biotechnology

Figure 5

From: Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

Figure 5

β-gal and SEAP production of Sf9, High Five, and Ao38 cells. (A) Beta-galactosidase production was measured from Ao38, High Five, and Sf9 cells at various time post infection (Days post infection) by virus βgal-AcMNPV. Ao38.SFM represents Ao38 cells grown in serum-free medium (Sf-900III medium) and all other samples were produced in TNMFH containing 10% FBS. Data points and bars represent averages and standard deviations, respectively, from triplicate infections. (B) Secreted human placental alkaline phosphatase (SEAP) was measured from Ao38, High Five, and Sf9 cells at various time post infection (Days post infection) by virus SEAP-AcMNPV. Ao38.SFM represents Ao38 cells grown in serum-free medium (Sf-900III medium) and all other samples were produced in TNMFH containing 10% FBS. Data points and bars represent averages and standard deviations, respectively, from triplicate infections.

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BMC Biotechnology

ISSN: 1472-6750

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