Figure 4From: α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabicaplants inhibits α-amylases from the coffee berry borer pestSouthern blot indicating the presence of α-AI1 in the genome of Coffea arabica T0 plants. P, positive control (α-AI1 Fragment, 735 bp); N, non-transformed coffee plant; 1 - 6, α-AI1 positive coffee plants (4150-bp fragment). Plant DNA extracts were digested with the enzymes PvuII, EcoRI and ClaI at the same time.Back to article page