Figure 4

Simulating a typical high-throughput cultivation process: effect on growth and product formation of Corynebacterium glutamicum pEKEx2-phoD-GFP. Transformed genetically identical C. glutamicum clones were grown on agar-plate. Sixty-two colonies from this plate were transferred to a MTP with toothpicks and cultivated over night. Glycerine was added to the MTP which was then frozen at -20°C. The cryoculture-MTP was thawed and mixed. Subsequently, 10 μL of each well were transferred to 190 μL medium in a preculture MTP. After cultivation over night 10 μL of each well were transferred to 190 μL medium in a main culture MTP. The following cultivation with induction was monitored with the BioLector and is shown above. A) Scattered light intensity. B) GFP fluorescence intensity. Vertical dashed line: time of induction with 0.5 mM IPTG after 5.25 h. Experimental conditions: modified Eikmanns mineral medium, filling volume per well 200 μL, shaking frequency 995 rpm, shaking diameter 3 mm, temperature 37°C, measurement with BioLector.