Figure 5
Scatter plot of average intensities ((S1+S2)/2) vs. variance (σ2) for the two control samples from the Incyte experiment. Average intensities were first sorted by magnitude, then averaged using a sliding window of 100 points; The same window was used to calculate σ2 for the corresponding sets of differences. A polynomial was fit to the data using least squares of form: y = 2 × 10-7x3 - 1.7 × 10-3x2 + 21.503x - 6548.3; R2 = 0.914. RU, relative units.