Cloning and characterization of an adenoviral vector for highly efficient and doxycycline – suppressible expression of bioactive human single – chain interleukin 12 in colon cancer

Background Interleukin-12 (IL-12) is well characterized to induce cellular antitumoral immunity by activation of NK-cells and T-lymphocytes. However, systemic administration of recombinant human IL-12 resulted in severe toxicity without perceptible therapeutic benefit. Even though intratumoral expression of IL-12 leads to tumor regression and long-term survival in a variety of animal models, clinical trials have not yet shown a significant therapeutic benefit. One major obstacle in the treatment with IL-12 is to overcome the relatively low expression of the therapeutic gene without compromising the safety of such an approach. Our objective was to generate an adenoviral vector system enabling the regulated expression of very high levels of bioactive, human IL-12. Results High gene expression was obtained utilizing the VP16 herpes simplex transactivator. Strong regulation of gene expression was realized by fusion of the VP16 to a tetracycline repressor with binding of the fusion protein to a flanking tetracycline operator and further enhanced by auto-regulated expression of its fusion gene within a bicistronic promoter construct. Infection of human colon cancer cells (HT29) at a multiplicity of infection (m.o.i.) of 10 resulted in the production of up to 8000 ng/106 cells in 48 h, thus exceeding any published vector system so far. Doxycycline concentrations as low as 30 ng/ml resulted in up to 5000-fold suppression, enabling significant reduction of gene expression in a possible clinical setting. Bioactivity of the human single-chain IL-12 was similar to purified human heterodimeric IL-12. Frozen sections of human colon cancer showed high expression of the coxsackie adenovirus receptor with significant production of human single chain IL-12 in colon cancer biopsies after infection with 3*107 p.f.u. Ad.3r-scIL12. Doxycycline mediated suppression of gene expression was up to 9000-fold in the infected colon cancer tissue. Conclusion VP16 transactivator-mediated and doxycycline-regulated expression of the human interleukin-12 gene enables highly efficient and tightly controlled cytokine expression in human cancer. These data illustrate the potential of the described adenoviral vector system for the safe and superior expression of therapeutic genes in the treatment of colorectal cancer and other malignancies.


Background
IL-12 has a major impact in the modulation of cellular immune response mechanisms. The heterodimeric cytokine enhances the proliferation and activation of natural killer cells (NK-cells) and the maturation of activated T lymphocytes to type 1 T helper cells (CD4 + ) and cytotoxic T lymphocytes (CD8 + ) [1][2][3][4] unveiling its potential for the treatment of infectious [5,6] and malignant diseases [7]. Induction of a cellular antitumoral immune response is directed not only against the primary lesion but also against distant organ metastases opening therapeutic options for those patients considered incurable at the present time. Tumor regression at the treatment site as well as in distant organ metastases was illustrated in 1997 utilizing a gene gun technique to incorporate plasmid DNA into intradermal tumors in mice [8]. In this particular tumor model IL-12 resulted in a superior antitumoral immune response and lasting systemic antitumoral immunity as compared with a variety of other cytokines (Interleukin-2, -4, -6, IFN-γ, TNF-α or GM-CSF). IL-12mediated antitumoral immune response was also illustrated in a variety of different murine tumor models [9][10][11][12][13][14].
Systemic application of recombinant IL-12 resulted in a broad and undirected stimulation of the cellular immune system lacking antitumoral efficacy. Toxicity was mainly contributed by the induction of interferon-γ with anti-IFN-γ antibodies abolishing the lethal effect of systemic administration of recombinant IL-12 in mice [15]. Interferon-γ induction also contributes in part to the induction of a cellular antitumoral immune response since anti-IFNγ antibodies diminish systemic antitumoral immunity [14]. Nevertheless, studies with IFN-γ receptor-knockout mice suggested an interferon-γ independent pathway of IL-12-mediated antitumoral immunity [16].
In humans the maximum tolerable dose (MTD) of IL-12 was determined to be 0.5 µg/kg body weight after intravenous administration [17,18] with reversion of advanced cancer associated defects in T-lymphocytes and NK cells [19]. Remarkably MTD was slightly lower after subcutaneous administration [20,21]. Repeated administration of IL-12 resulted in an increasing tolerance (priming effect) [20,[22][23][24] which was in part due to increased STAT-4 (IFN-γ transcription activator) degradation.
In Hodgkin's lymphoma repeated intravenous or subcutaneous administration of recombinant IL-12 resulted in a 21% response rate with partial (PR) and complete regression (CR) [25]. Repeated  Gene therapeutic approaches utilizing the coexpression of both subunits for the generation of bioactive IL-12 were compromised by the formation of inhibitory p40 homodimers. This interference was prevented by the introduction of IL-12 fusion genes [34,[41][42][43]. The potential of these constructs integrated in adenoviral expression systems for the treatment of gastrointestinal tumors was illustrated recently [44,45].
Considering the potential toxicity related to the expression of IL-12 and the necessity of high intratumoral concentrations, the scope of this study was to construct an adenoviral vector for highly efficient and regulated expression of a human single-chain IL-12. A modified E. coli operator system was utilized for tetracycline suppressible expression. High gene expression was enabled by the use of a VP16 herpes simplex virus transactivator fused to the E. coli Tet-repressor. Positioning of the trans-activating fusion protein was realized by Tet-operator upstream of the gene of interest [46]. This system was further enhanced by integration of a bidirectional promoter also expressing the transactivator in the absence of doxycycline [47]. In the presence of low concentrations of doxycycline the fusion protein immediately dissociates from the Tetoperator thru binding of doxycycline to the Tet-repressor domain with drastic reduction of VP16 mediated gene expression.

Construction of suppressible vectors for the expression of human IL-12
The adenoviral expression plasmid pAd.3r-hscIL-12 was constructed as described, and corresponding recombinant E1/E3 deleted adenoviral vectors were generated by calcium phosphate mediated cotransfection with pBHG10 in 293-cells (figure 1). Plaques were purified and the adenovirus was amplified in 293-cells. The adenoviral titer was determined utilizing standard plaque assay procedures.

Dox-dependent expression of human single-chain IL-12
We were able to illustrate the high adenoviral transduction efficacy in HT29 human colon cancer cells in previous publications [45,49,50]. These cells were infected with Ad.3r-hscIL12 at an m.o.i. of 10 followed by incubation for 48 hours at various concentrations of doxycycline. Human single-chain IL-12 was then determined in the cell lysate and the cell free culture supernatant. Substantial suppression was obtained at doxycycline concentrations as low as 3 ng/ml with maximum suppression levels of 2200-fold for IL-12 in the supernatant and 5000-fold in the cell lysate at 30 ng/ml doxycycline (figure 2). In the absence of dox, infection at an m.o.i. of 10 resulted in 2% IL-12 in relation to total soluble cellular protein (data not shown).
Western blot analysis revealed significant suppression of TetR as detected with a TetR monoclonal antibody [46], illustrating down regulation of the transactivator proportional to the suppression of IL-12 expression in the presence of doxycycline (figure 3).

Comparison of bioactivity
Bioactivity of adenovirally-expressed human single-chain IL-12 was compared with purified heterodimeric p40/p35 standard in order to estimate the biological response in a clinical setting. Bioactivity was first evaluated by IFN-γ induction in human mononuclear cells after conditioning with CD3-and CD28-antibodies. Pretreated MNC were then incubated with hscIL-12 from the supernatant of adenovirally infected HT29 cells or with equivalent concentrations of a human heterodimeric IL-12 WHO-standard. Twenty-four hours after incubation at 37°C, Doxycycline dependent IL-12 expression expressed human interferon-γ was quantified by ELISA ( figure 4). Bioactivity profiles did not reveal a significant difference. Interferon-γ induction was observed at IL-12 concentrations as low as 10 pg/ml with maximum induction at 1 ng/ml. ED 50 was estimated to be 54 -64 pg/ml IL-12 resulting in the induction of interferon-γ at 3000 pg/ ml. Basal induction of interferon-γ in the absence of IL-12 was 1400 pg/ml, mainly due to prestimulation of MNC with CD3-and CD28-antibodies.
To further delineate whether the IFN-γ production was induced by IL-12, pre-stimulated MNC were incubated with a) native medium, b) heterodimeric IL-12, or c) viral IL-12 for 24 hours and brefeldin A for 20 hours to prevent the release of intracellular proteins including IFN-γ. The MNC were subsequently labeled by surface staining with fluorochrome-conjugated mAb's detecting CD3-APC/ CD8 and CD69 followed by intracellular labeling with an anti-IFN-γ -FITC mAb and analyzed by flow cytometry. Recently activated T4-and T8-cells as defined by the expression of CD69 were analyzed for their IFN-γ producing profile. A representative IFN-γ expression analysis is shown in Figure 5A.

Coxsackie-adenovirus receptor expression and adenoviral transduction in human colon cancer
Demonstrating high adenoviral transduction efficiency in a variety of human colon cancer cell lines [45], the question remained whether native human cancer tissue shows equal susceptibility towards adenoviral infection. Since adenoviral infection is mainly mediated by the Coxsackie-Adenovirus receptor (CAR), human colon and colon cancer tissues were stained for CAR expression. As illustrated in figure 6, normal colon epithelium as well as human colon cancer cells revealed high expression of the CAR using a monoclonal CAR-antibody and APAAP staining with no expression in the adjacent tissue. Staining applying a detection system based on horseradish peroxidase  Figure 4 Comparison of bioactivity. Comparison of interferon-γ induction by incubation of mononuclear cells (MNC) with either adenovirally generated single-chain human interleukin-12 or purified standardized heterodimeric human interleukin-12 as quantified by ELISA. resulted in identical distribution (data not shown). Figure  7 illustrates transgene expression after infection of human colon cancer specimens with doxycycline suppressible adenoviral vectors. Luciferase and human IL-12 were significantly expressed in human colon cancer tissue in the absence of doxycycline. Addition of dox at a concentration of 2 µg/ml resulted in a 1200-fold suppression for non-secreted luciferase and 9700-fold suppression for secreted single-chain human IL-12.

Discussion
Recent clinical trials utilizing recombinant IL-12 in the experimental treatment of human cancer illustrate the potential hazards of these approaches. Severe side effects such as hemorrhagic colitis and sepsis indicate the unacceptable risk associated with systemic and repeated administration of recombinant IL-12 in the treatment of cancer [17]. Local application is limited by the short halflife of the cytokine and showed some efficacy only in a sparse number of tumor entities. Therefore, local expression of an IL-12 gene is a promising approach to avoid such problems. Vector systems for the expression of human IL-12 did not result in significant clinical response yet, which is most likely due to weak transgene expression.
We were able to construct adenoviral vectors addressing these issues by incorporation of a highly potent and doxycycline-regulated transactivator for the expression of human single-chain IL-12. Infection of human colon cancer cells (HT29) at an m.o.i. of 10 resulted in the expression of 8000 ng/10 6 cells in 48 h. To our knowledge, these expression levels exceed any adenoviral vectors expressing human IL-12 as well as stably transduced cell lines published so far. Doxycycline concentrations as low as 30 ng/ ml resulted in suppression levels of up to 5000-fold. With peak concentrations of 2.6 µg/ml 2 hours after oral application of 200 mg doxycycline and average serum levels of 1.11 µg/ml after 100 mg doxycycline daily [51], suitable concentrations of doxycycline are quickly obtainable for efficient and timely suppression of transgene expression. Addition of doxycycline to the drinking water completely abolished the otherwise lethal effect of these systemically administered murine IL-12 expressing vectors in mice (Block et al., Manuscript submitted).
Interferon-γ induction in human mononuclear cells after incubation with adenovirally expressed human singlechain IL-12 did not differ from induction with purified p35/p40 heterodimer at various concentrations. These results together with the comparative FACS analysis show similar bioactivity. Inhibition of bioactivity through the formation of p40 homodimers was avoided by expressing a IL-12 fusion protein rather than the separate subunits e.g. by utilizing an internal ribosome entry site [52,53]. Furthermore, the human single-chain IL-12 contains a secretory leader domain, resulting in efficient secretion Ex vivo expression of luciferase and IL-12 Figure 7 Ex vivo expression of luciferase and IL-12. Luciferase and IL12 expression after ex vivo infection of human colon cancer biopsies with Ad.3r-luc or Ad.3r-hscIL12 in the presence (2 µg/ml) or absence of doxycycline. independent of posttranslational processing and p35 subunit glycosylation [54,55]. Adenoviral transduction of human tissue requires the expression of the coxsackie adenovirus receptor (CAR) with vector internalization depending on alphavbeta3and alphavbeta5-integrins. Expression of the CAR was shown in human colon epithelium as well as colon cancer illustrating the feasibility of adenoviral transduction and adenovirally mediated transgene expression. Adenoviral infection of freshly derived human colon cancer biopsies with 3*10 7 p.f.u. demonstrated efficient ex vivo transgene expression as well as highly significant doxycycline-mediated suppression in tumor tissue. Colon cancer differentiation dependent CAR expression and consecutive transgene expression remain to be determined and is currently under investigation.

CAR expression in human tissue
These data further support the use of doxycycline-suppressible, highly efficient adenoviral vector systems for the treatment of human cancer. Vector related toxicity could probably be avoided by applying a significantly lower viral dose since transgene expression within these new promoter systems was shown to be more than 4000fold higher as compared with the CMV-promoter commonly used in clinical trials. Tumor regression and systemic immunity after high intratumoral human IL-12 expression have to be addressed in a clinical setting. Even though oral administration of doxycycline can dramatically reduce IL-12 expression and related toxicity in mice, doxycycline mediated suppression of IL-12 expression in humans remains to be investigated. Serum half life of recombinant human IL-12 was determined to be 5 to 21 hours [18,20,23,56], thus possibly requiring additional precautions such as the temporary application of neutralizing anti IL-12 or interferon-γ antibodies in order to inactivate circulating cytokines.

Conclusion
With expression levels exceeding the widely used viral CMV-promoter, combined with tight regulation of gene expression by low concentrations of doxycycline, we are confident that these adenoviral vectors will add to the feasibility and safety in the immunotherapy of colorectal cancer as well as other malignancies.

Human colon cancer specimen
In accordance with national ethical guidelines issued by the German research foundation patients were informed about the study and gave their written consent at a minimum of 24 hours prior to surgery. Colon cancer specimens were removed according to standard surgical procedures. At least 8 colon cancer biopsies were taken from the vital area of each freshly prepared human colon cancer tissue utilizing a medium size endoscopic biopsy forceps. These samples were washed three times with PBS prior to ex vivo transduction with viral vectors.

Construction of adenoviral expression plasmids
Preparation of plasmid DNA was performed utilizing a modified alkaline lysis protocol followed by purification through anion exchange column (Qiagen, Valencia, CA). Plasmid DNA was propagated in DH5a E. coli. Restriction endonucleases were obtained from Boehringer Mannheim (Ingelheim, Germany) and New England Biolabs (Ipswich, MA). The plasmid pSFG-hIL12.p40.linkerdelta.p35 containing a human IL-12 fusion protein, was kindly provided by R.C. Mulligan (Children's hospital, Boston, MA) [42]. The backbone plasmid pAd.3r.pA was obtained by digestion of pAd.CMV.pA [45] with XbaI, blunting and SalI in order to remove the CMV promoter, the multiple cloning site, and the polyA. The previously described bicistronic promoter element was then released from pBIG3r after digestion with PvuII and SalI and ligated into the adenoviral expression plasmid. The cDNA encoding for human single-chain IL-12 (hscIL12) was released from pSFG-hIL12.p40.linker-delta.p35 through digestion with NcoI, refilling with T4 polymerase and subsequent digestion with NheI. pAd.3r.pA was digested with BamHI, refilled and digested with SpeI with ligation of the previously released hscIL12 encoding cDNA in between 3r and the poly A. The resulting adenoviral expression plasmid pAd.3r.hscIL-12 contains the cDNA encoding for a human IL-12 fusion protein under control of a bidirectional, doxycycline-suppressible promoter element, 5'flanked by base pairs 1 to 456 and 3'-flanked by base pairs 3346 to 5864 of the Ad5 genome. Minimal TK-promoterdriven expression of the tTA was antiparallel and minimal CMV-promoter-driven expression of the human singlechain IL-12 gene was parallel to adenoviral E1 transcription. The complete expression cassette was sequenced and a base exchange repair performed at position 4059 (C/T) by PCR.

Generation and amplification of the corresponding adenoviral vector
Recombinant, E1-and E3-deleted adenoviral vectors were generated by calcium phosphate mediated coprecipita-tion and homologous recombination of pAd.3r.hscIL12 with pBHG10 in 293-cells as described elsewhere [48]. Adenoviral vectors were propagated in 293-cells and purified by CsCl density gradient centrifugation. Titration of the purified virus was performed by plaque assay. The obtained titer for Ad.3r.hscIL12 was 3.6*10 9 p.f.u./ml. Viral DNA was isolated using the DNA Blood Mini Kit (Qiagen) and sequencing of the insert was done with an ABI 377 Sequenator (Perkin Elmer, Norwalk, CT) after asymmetrical amplification and labeling with BigDye Terminator Cycle Sequencing Mix (Applied Biosystems, Foster City, CA).

Transfection and quantification of transgene expression
HT29 human colon cancer cells were seeded in six-well plates at a concentration of 10 6 cells/well 6 hours prior to infection. Tumor cells were infected in triplicates with purified recombinant adenovirus in 500 µl serum-free media for 1 hour followed by incubation with complete media and different doxycycline concentrations for another 48 hours. Human single-chain IL-12 was quantified in cell-free culture supernatant and cell lysate 48 hours after infection using a human IL-12 (P70) OptEIA ELISA (Pharmingen, San Jose, CA) assuming equal immunoreactivity as compared with the human p40/p35 heterodimer. Cells were lysed using 150 µl of culture lysis reagent (Promega, Madison, WI) according to the manufacturers protocol and IL-12 quantified as previously described.
Human colon cancer biopsies were infected with either Ad.3r-luc or Ad.hscIL12 for 4 hours followed by incubation in HGDMEM supplemented with certified tetracycline free fetal bovine serum (Clontech, Palo Alto, CA), 1% L-glutamine and 1% penicillin/streptomycin (Gibco) in the presence or absence of Doxycycline (2 µg/ml) for 48 hours. IL-12 was determined in the supernatant as described.
Luciferase expression was quantified by measuring luciferase activity in 20 µl of cell culture supernatant with a Bertold LB9507 luminometer and luciferase assay substrate (Promega). Standard curves were generated using recombinant firefly luciferase (Promega) diluted with CCLR to concentrations from 1 pg/ml to 300 ng/ml and a two-phase exponential association curve fitting was performed due to saturation at higher concentrations using the Prism software package (GraphPad Software, Inc, San Diego, CA).

SDS-PAGE and immunoblotting
Cell lysates were resuspended in laemmli loading buffer and separated using gel electrophoresis (Nu PAGE Novex Bis-Tris Gel, Invitrogen). After blotting on Hybound-P PVDF membranes (Amersham, Buckinghamshire, UK), proteins were labeled with anti-β-actin (Sigma, St. Louis, MI) and anti-TetR (Clontech) monoclonal antibodies. Blots were then incubated with a peroxidase-linked sheep anti-mouse secondary antibody (Amersham) and proteins visualized through chemiluminescence by an ECL Western blotting detection Kit (Amersham).

Bioassay
In order to characterize the bioactivity of virally expressed human single-chain IL-12, human peripheral blood mononuclear cells (MNCs) were purified from whole blood after written consent and in accordance with national ethical guidelines, following the LeucoSep-procedure (Greiner, Frickenhausen, Germany). Cell culture bottles were coated with anti-human CD3 antibodies (10 µg/ml in PBS, #MAB100, R&D Systems, Minneapolis, MN) and separated MNC were then incubated in the presence of an anti-human CD28 antibody (#AF-342-PB, R&D Systems) at 0.5 µg/ml for three days. Prestimulated MNC were seeded into 96 well plates at 4 × 10 4 cells/well and incubated in 125 µl with various concentrations of IL-12 either from adenovirally infected HT29 cell culture supernatant or WHO reference standard (#95/544, NIBSC, Herfordshire, UK). 24 hours later human interferon-γ was quantified in cell free supernatant using a human IFN-γ OptEIA ELISA (Pharmingen).

FACS-analysis
CD3-and CD28-prestimulated MNC were seeded into 6 well plates at 10 6 cells/well and incubated with different IL-12 preparations at 100 pg/ml for 24 hours. 4 hours after the addition of IL-12 the golgi apparatus was blocked by brefeldin A (BFA) to prevent the export of newly synthesized INF-γ. Cells were then labeled utilizing the Fastimmune Intracellular Cytokine Detection Kit (#346049, Becton-Dickinson Biosciences, San Jose, CA). Briefly, cells were washed in PBS containing 0.5% fetal bovine serum and 0.1% NaN 3 and resuspended in 0.5 ml FACS-Perm prior to incubation at room temperature for 10 minutes. Permeable cells were then stained with 20 µl of a DB Fastimmune anticytokine antibody solution (Anti-Hu-IFN-γ FITC/CD69PE/CD8PerCP-Cy5.5/CD3APC) for 30 minutes, washed and fixated in PFA (1% paraformaldehyde in PBS). Intracellular cytokine expression was performed by FACScalibur flow cytometry (BD Biosciences) and analyzed by cellquest software.

Immunohistochemistry
Frozen sections of normal colon tissue and colon cancer tissue with a thickness between 5 and 10 microns were dried on coated slides at room temperature over night. After fixation with 4% paraformaldehyde for 10 minutes and incubation with 1% FCS for 30 minutes at room temperature, sample sections were then incubated with the mouse IgG 1 monoclonal antibody Anti-CAR (clone RmcB, Upstate, NY) at 1 µg/ml in TBS supplemented with 1% fetal bovine serum for 1 hour at room temperature. Samples were incubated for 30 minutes at room temperature with rabbit anti-mouse IgG at 5 µg/ml. Immunostaining according to the alkaline phosphatase/anti-alkaline phosphatase (APAAP) protocol was done by incubating the sections with a mouse monoclonal APAAP complex (Dako, dilution 1:50) for 30 minutes at room temperature. The enzyme reaction was then detected by new fuchsin (Merck, Darmstadt, Germany), naphthol AS-BI (Sigma) and levamisol (Sigma) and sections were finally counterstained with hematoxylin.

Authors' contributions
HW carried out the construction and characterization of human IL-12 expressing adenoviral vectors, TK and IS participated in the FACS analysis. KK carried out the ex vivo studies infecting human cancer tissue and quantifying gene expression, FT participated in the SDS page and immunoblotting, HS carried out the histochemistry and helped drafting the manuscript. AB conceived of the study, participated in its design and coordination and drafted the manuscript. All authors read and approved the final manuscript.