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  1. Content type: Methodology article

    Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of i...

    Authors: Hanna Amelina, Vera Moiseeva, Laura Catharine Collopy, Siân Rosanna Pearson, Christine Anne Armstrong and Kazunori Tomita

    Citation: BMC Biotechnology 2016 16:76

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  2. Content type: Research article

    Transglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptom...

    Authors: Song Liu, Miao Wang, Guocheng Du and Jian Chen

    Citation: BMC Biotechnology 2016 16:75

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  3. Content type: Research article

    The isolation of unknown DNA sequences flanked by known sequences is an important task in the event-specific detection of GMOs. None of event-specific detection method was developed based on the junction seque...

    Authors: Hongwei Gao, Xiaofan Yu, Tingting Deng, Min Sun, Xizhi Xiao, Xin Huang, Ying Chen and Ronggui Li

    Citation: BMC Biotechnology 2016 16:74

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  4. Content type: Research article

    Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan. Appended ...

    Authors: Shiping Liu and Shaojun Ding

    Citation: BMC Biotechnology 2016 16:73

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  5. Content type: Research article

    A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of ...

    Authors: Guojun Wang, Ting Zhang, Haifeng Huang, Shurong Hou, Xiabin Chen, Fang Zheng and Chang-Guo Zhan

    Citation: BMC Biotechnology 2016 16:72

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  6. Content type: Research article

    Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter d...

    Authors: Steven C. L. Ho, Esther Y. C. Koh, Benjamin P. C. Soo, Mariati, Sheng-Hao Chao and Yuansheng Yang

    Citation: BMC Biotechnology 2016 16:71

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  7. Content type: Research article

    Ethyl (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE) is a versatile chiral precursor for many pharmaceuticals. Although several biosynthesis strategies have been documented to convert ethyl 4-chloro-3-oxobutanoate (CO...

    Authors: Xiang Chen, Zhi-Qiang Liu, Chao-Ping Lin and Yu-Guo Zheng

    Citation: BMC Biotechnology 2016 16:70

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  8. Content type: Research article

    Abiotic stresses are serious threats to plant growth, productivity and result in crop loss worldwide, reducing average yields of most major crops. Although abiotic stresses might elicit different plant respons...

    Authors: Yuanyuan Bu, Bo Sun, Aimin Zhou, Xinxin Zhang, Testuo Takano and Shenkui Liu

    Citation: BMC Biotechnology 2016 16:69

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  9. Content type: Research article

    RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five treh...

    Authors: Bin Tang, Ping Wei, Lina Zhao, Zuokun Shi, Qida Shen, Mengmeng Yang, Guoqiang Xie and Shigui Wang

    Citation: BMC Biotechnology 2016 16:67

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  10. Content type: Methodology article

    Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is ...

    Authors: Alexander Falkenhagen, Sabah Asad, Stanley E. Read and Sadhna Joshi

    Citation: BMC Biotechnology 2016 16:66

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  11. Content type: Research article

    Targeted therapies for cancer, especially the malignant cancer, are always restricted by the deficiency of tumor-specific drug delivery methods. Subtilase cytotoxic is a virulent cytotoxin, and the subunit A (...

    Authors: Lichao Zhang, Zongwei Li, Tonglin Shi, Xiaoqin La, Hanqing Li and Zhuoyu Li

    Citation: BMC Biotechnology 2016 16:65

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  12. Content type: Methodology article

    Infectious clones are fundamental tools for the study of many viruses, allowing for efficient mutagenesis and reproducible production of genetically-defined strains. For the large dsDNA genomes of the herpesvi...

    Authors: Alexsia L. Richards, Patricia J. Sollars and Gregory A. Smith

    Citation: BMC Biotechnology 2016 16:64

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  13. Content type: Research article

    Transcription factors (TFs) are effectors of cell signaling pathways that regulate gene expression. TF networks are highly interconnected; one signal can lead to changes in many TF levels, and one TF level can...

    Authors: Betul Bilgin, Aritro Nath, Christina Chan and S. Patrick Walton

    Citation: BMC Biotechnology 2016 16:62

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  14. Content type: Research article

    Diols are important monomers for the production of plastics and polyurethanes, which are widely used in our daily life. The medium-chain diols with one hydroxyl group at its subterminal end are able to confer ...

    Authors: Yanning Zheng, Lingling Li, Qiang Liu, Haibo Zhang, Yujin Cao, Mo Xian and Huizhou Liu

    Citation: BMC Biotechnology 2016 16:61

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  15. Content type: Methodology article

    Selected microRNAs (miRNAs) that are abnormally expressed in the serum of patients with lung cancer have recently been proposed as biomarkers of this disease. The measurement of circulating miRNAs, however, re...

    Authors: Paola Campomenosi, Elisabetta Gini, Douglas M. Noonan, Albino Poli, Paola D’Antona, Nicola Rotolo, Lorenzo Dominioni and Andrea Imperatori

    Citation: BMC Biotechnology 2016 16:60

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  16. Content type: Methodology article

    CRISPR/Cas9 genome editing strategy has been applied to a variety of species and the tRNA-processing system has been used to compact multiple gRNAs into one synthetic gene for manipulating multiple genes in rice.

    Authors: Weiwei Qi, Tong Zhu, Zhongrui Tian, Chaobin Li, Wei Zhang and Rentao Song

    Citation: BMC Biotechnology 2016 16:58

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  17. Content type: Methodology article

    Large efforts have focused on screening for genes involved in the virulence and pathogenicity of Verticillium dahliae, a destructive fungal pathogen of numerous plant species that is difficult to control once the...

    Authors: Latifur Rehman, Xiaofeng Su, Huiming Guo, Xiliang Qi and Hongmei Cheng

    Citation: BMC Biotechnology 2016 16:57

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  18. Content type: Research article

    Foot-and-mouth disease (FMD) is an acute, highly contagious disease that infects cloven-hoofed animals. Vaccination is an effective means of preventing and controlling FMD. Compared to conventional inactivated...

    Authors: Yan Xiao, Hong-Ying Chen, Yuzhou Wang, Bo Yin, Chaochao Lv, Xiaobing Mo, He Yan, Yajie Xuan, Yuxin Huang, Wenqiang Pang, Xiangdong Li, Y. Adam Yuan and Kegong Tian

    Citation: BMC Biotechnology 2016 16:56

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  19. Content type: Research article

    L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. In previous reports, AIs from thermophilic bacterial strains had been wildly researched, but the brownin...

    Authors: Wending Mei, Lu Wang, Ying Zang, Zhaojuan Zheng and Jia Ouyang

    Citation: BMC Biotechnology 2016 16:55

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  20. Content type: Methodology article

    Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appro...

    Authors: P. Scott Pine, Sarah A. Munro, Jerod R. Parsons, Jennifer McDaniel, Anne Bergstrom Lucas, Jean Lozach, Timothy G. Myers, Qin Su, Sarah M. Jacobs-Helber and Marc Salit

    Citation: BMC Biotechnology 2016 16:54

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  21. Content type: Methodology article

    Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid trans...

    Authors: Kalpana Nanjareddy, Manoj-Kumar Arthikala, Lourdes Blanco, Elizabeth S. Arellano and Miguel Lara

    Citation: BMC Biotechnology 2016 16:53

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  22. Content type: Research article

    Succinate is a kind of industrially important C4 platform chemical for synthesis of high value added products. Due to the economical and environmental advantages, considerable efforts on metabolic engineering ...

    Authors: Ying Zhao, Chang-Song Wang, Fei-Fei Li, Zhen-Ning Liu and Guang-Rong Zhao

    Citation: BMC Biotechnology 2016 16:52

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  23. Content type: Research article

    Dioxins are one of the most toxic groups of persistent organic pollutants. Their bioaccumulation through the food chain constitutes a potential risk for human health. Upon cell entry, dioxins bind specifically...

    Authors: Walaa Faiad, Abdulsamie Hanano, Mohamed Maher Kabakibi and Abdul Qader Abbady

    Citation: BMC Biotechnology 2016 16:51

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  24. Content type: Research article

    Four antigenically distinct serotypes (1–4) of dengue viruses (DENVs) cause dengue disease. Antibodies to any one DENV serotype have the potential to predispose an individual to more severe disease upon infect...

    Authors: Ankur Poddar, Viswanathan Ramasamy, Rahul Shukla, Ravi Kant Rajpoot, Upasana Arora, Swatantra K. Jain, Sathyamangalam Swaminathan and Navin Khanna

    Citation: BMC Biotechnology 2016 16:50

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  25. Content type: Research article

    Microalgae have been recognized as a good food source of natural biologically active ingredients. Among them, the green microalga Euglena is a very promising food and nutritional supplements, providing high value...

    Authors: Min Zeng, Wenlong Hao, Yongdong Zou, Mengliang Shi, Yongguang Jiang, Peng Xiao, Anping Lei, Zhangli Hu, Weiwen Zhang, Liqing Zhao and Jiangxin Wang

    Citation: BMC Biotechnology 2016 16:49

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  26. Content type: Research article

    As the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production. Cassava residues (CR) are byproducts of the cassava starch industry ...

    Authors: Li Ji, Tianran Zheng, Pengxiang Zhao, Weiming Zhang and Jianxin Jiang

    Citation: BMC Biotechnology 2016 16:48

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  27. Content type: Research article

    The ATP-binding cassette (ABC) transporter gene superfamily is ubiquitous among extant organisms and prominently represented in plants. ABC transporters act to transport compounds across cellular membranes and...

    Authors: Thomas S. Lane, Caroline S. Rempe, Jack Davitt, Margaret E. Staton, Yanhui Peng, Douglas Edward Soltis, Michael Melkonian, Michael Deyholos, James H. Leebens-Mack, Mark Chase, Carl J. Rothfels, Dennis Stevenson, Sean W. Graham, Jun Yu, Tao Liu, J. Chris Pires…

    Citation: BMC Biotechnology 2016 16:47

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  28. Content type: Research article

    Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention a...

    Authors: Yanli Sun, Yanhua Sun, Ronglan Zhao and Kunshan Gao

    Citation: BMC Biotechnology 2016 16:46

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  29. Content type: Research article

    Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass...

    Authors: Dennis Lamers, Nick van Biezen, Dirk Martens, Linda Peters, Eric van de Zilver, Nicole Jacobs-van Dreumel, René H. Wijffels and Christien Lokman

    Citation: BMC Biotechnology 2016 16:45

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  30. Content type: Research article

    Tissue engineering and bone substitutes are subjects of intensive ongoing research. If the healing of bone fractures is delayed, osteoinductive materials that induce mesenchymal stem cells (MSCs) to form bone ...

    Authors: Steffen Kissling, Michael Seidenstuecker, Ingo H. Pilz, Norbert P. Suedkamp, Hermann O. Mayr and Anke Bernstein

    Citation: BMC Biotechnology 2016 16:44

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  31. Content type: Research article

    In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the β-actin gene. This high level of the gene expression has attracted our atten...

    Authors: Roman A. Komakhin, Denis A. Vysotskii, Rahim R. Shukurov, Vera D. Voblikova, Vera V. Komakhina, Svetlana R. Strelnikova, Ekaterina M. Vetchinkina and Alexey V. Babakov

    Citation: BMC Biotechnology 2016 16:43

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  32. Content type: Research article

    L-(+)-tartaric acid (L-TA) is an important organic acid, which is produced from the cream of tartar or stereospecific hydrolysis of the cis-epoxysuccinate. The former method is limited by the availability of raw ...

    Authors: Jianfeng Yuan, Mianbin Wu, Jianping Lin and Lirong Yang

    Citation: BMC Biotechnology 2016 16:42

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  33. Content type: Research article

    The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will p...

    Authors: Justin E. Anderson, Jean-Michel Michno, Thomas J. Y. Kono, Adrian O. Stec, Benjamin W. Campbell, Shaun J. Curtin and Robert M. Stupar

    Citation: BMC Biotechnology 2016 16:41

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  34. Content type: Research

    NAC proteins (NAM (No apical meristem), ATAF (Arabidopsis transcription activation factor) and CUC (cup-shaped cotyledon)) are plant-specific transcription factors reported to be involved in regulating growth,...

    Authors: Hifzur Rahman, Valarmathi Ramanathan, Jagedeeshselvam Nallathambi, Sudhakar Duraialagaraja and Raveendran Muthurajan

    Citation: BMC Biotechnology 2016 16(Suppl 1):35

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    This article is part of a Supplement: Volume 16 Supplement 1

  35. Content type: Research

    The twin problem of shortage in fossil fuel and increase in environmental pollution can be partly addressed by blending of ethanol with transport fuel. Increasing the ethanol production for this purpose withou...

    Authors: Saranyah Kannuchamy, Nisha Mukund and Lilly M. Saleena

    Citation: BMC Biotechnology 2016 16(Suppl 1):34

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    This article is part of a Supplement: Volume 16 Supplement 1

  36. Content type: Research article

    The valuable role of immunotherapy in treating autoimmune diseases is increasingly recognized by those involved in the research and clinical application of new biopharmaceuticals products. However, many aspect...

    Authors: Gecilmara Salviato Pileggi, Aline Dayana Clemencio, Thiago Malardo, Sonir R Antonini, Vania Luiza Deperon Bonato, Wendy Martin Rios and Celio L Silva

    Citation: BMC Biotechnology 2016 16:40

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  37. Content type: Research article

    Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric pathogen of swine. The spike glycoprotein (S) of PEDV is the major immunogenic determinant that plays a pivotal role in the induction of ne...

    Authors: Da-Chuan Piao, Do-Woon Shin, In-Seon Kim, Hui-Shan Li, Seo-Ho Oh, Bijay Singh, S. Maharjan, Yoon-Seok Lee, Jin-Duck Bok, Chong-Su Cho, Zhong-Shan Hong, Sang-Kee Kang and Yun-Jaie Choi

    Citation: BMC Biotechnology 2016 16:39

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  38. Content type: Research article

    The whole-cell lipase from Burkholderia cepacia has been used as a biocatalyst in organic synthesis. However, there is no report in the literature on the component or the gene sequence of the cell-bound lipase fr...

    Authors: Zhengyu Shu, Hong Lin, Shaolei Shi, Xiangduo Mu, Yanru Liu and Jianzhong Huang

    Citation: BMC Biotechnology 2016 16:38

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  39. Content type: Methodology article

    Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a...

    Authors: Asuka Mukai, Aya Ichiraku and Kazuki Horikawa

    Citation: BMC Biotechnology 2016 16:37

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  40. Content type: Methodology article

    G protein-coupled receptors (GPCRs) are ubiquitous surface proteins mediating various biological responses and thus, important targets for therapeutic drugs. GPCRs individually produce their own signaling as w...

    Authors: Yuki Nonobe, Tomoki Yokoyama, Yuji Kamikubo, Sho Yoshida, Nozomi Hisajima, Hiroaki Shinohara, Yuki Shiraishi, Takashi Sakurai and Toshihide Tabata

    Citation: BMC Biotechnology 2016 16:36

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  41. Content type: Research article

    Angiotensinogen (ANG) is a macromolecular precursor of angiotensin, which regulates blood pressure and electrolyte balance. ANG is specifically cleaved by renin, an aspartic protease, to initiate the angiotens...

    Authors: Shinji Yamashita, Naoya Shibata, Akiyoshi Boku-Ikeda, Erika Abe, Ayumi Inayama, Takashi Yamaguchi, Ayano Higuma, Kaoru Inagaki, Tomoyo Tsuyuzaki, Satoshi Iwamoto, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Kazal Boron Biswas, A. H. M. Nurun Nabi, Tsutomu Nakagawa…

    Citation: BMC Biotechnology 2016 16:33

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  42. Content type: Research article

    Fetal bovine serum (FBS) contains a wide range of growth factors, hormones, vitamins, amino acids, fatty acids and trace elements required for cell growth. It was shown that animal sera contain also extracellu...

    Authors: Hala Aswad, Audrey Jalabert and Sophie Rome

    Citation: BMC Biotechnology 2016 16:32

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  43. Content type: Research article

    Vanillin, a type of phenolic released during the pre-treatment of lignocellulosic materials, is toxic to microorganisms and therefore its presence inhibits the fermentation. The vanillin can be reduced to vani...

    Authors: Xinning Wang, Zhenzhen Liang, Jin Hou, Xiaoming Bao and Yu Shen

    Citation: BMC Biotechnology 2016 16:31

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  44. Content type: Research article

    Diabetes and its concurrent complications impact a significant proportion of the population of the US and create a large financial burden on the American health care system. FDA-approved maggot debridement the...

    Authors: Rebecca J. Linger, Esther J. Belikoff, Ying Yan, Fang Li, Holly A. Wantuch, Helen L. Fitzsimons and Maxwell J. Scott

    Citation: BMC Biotechnology 2016 16:30

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  45. Content type: Methodology article

    Research into gene expression enables scientists to decipher the complex regulatory networks that control fundamental biological processes. Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method...

    Authors: Nicky Boulter, Francia Garces Suarez, Stephen Schibeci, Trevor Sunderland, Ornella Tolhurst, Tegan Hunter, George Hodge, David Handelsman, Ulla Simanainen, Edward Hendriks and Karen Duggan

    Citation: BMC Biotechnology 2016 16:27

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