Articles
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Citation: BMC Biotechnology 2019 19(Suppl 1):78
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Tuning the performance of CAR T cell immunotherapies
Simultaneous advances in gene editing, T cell engineering and biotechnology currently provide an opportunity for rapid progress in medicine. The approval of chimeric antigen receptor (CAR) T cell therapies by ...
Citation: BMC Biotechnology 2019 19:84 -
LC-MSMS based screening of emerging pollutant degradation by different peroxidases
The presence of a wide range of bioactive organic pollutants in wastewater and municipal water sources is raising concerns about their potential effects on humans. Not surprisingly, various approaches are bein...
Citation: BMC Biotechnology 2019 19:83 -
Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis
Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secret...
Citation: BMC Biotechnology 2019 19:82 -
Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein
Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new ...
Citation: BMC Biotechnology 2019 19:81 -
A simple method for in vitro preparation of natural killer cells from cord blood
Cord Blood (CB) has been considered a promising source of natural killer (NK) cells for cellular immunotherapy. However, it is difficult to expand the large numbers of highly pure NK cells from CB without cell...
Citation: BMC Biotechnology 2019 19:80 -
Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When p...
Citation: BMC Biotechnology 2019 19:79 -
Correction to: A new primer construction technique that effectively increases amplification of rare mutant templates in samples
Following publication of the original article [1], the author informed us that the legend for Fig. 2 was incorrect.
Citation: BMC Biotechnology 2019 19:77 -
Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis
Corynebacterium ammoniagenes is an important industrial organism that is widely used to produce nucleotides and the potential for industrial production of coenzyme A by C. ammoniagenes ATCC 6871 has been shown. H...
Citation: BMC Biotechnology 2019 19:76 -
Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)
DNA transposon-based vectors are effective nonviral tools for gene therapy and genetic engineering of cells. However, promoter DNA methylation and a near-random integration profile, which can result in transge...
Citation: BMC Biotechnology 2019 19:75 -
Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects
Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are required. The CRISPR/Cas9 system is a powerful, highly efficient and easily manipulated...
Citation: BMC Biotechnology 2019 19:74 -
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection
Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assa...
Citation: BMC Biotechnology 2019 19:73 -
Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid production
In filamentous fungi, transport of organic acids across the mitochondrial membrane is facilitated by active transport via shuttle proteins. These transporters may transfer different organic acids across the me...
Citation: BMC Biotechnology 2019 19:72 -
gRNA validation for wheat genome editing with the CRISPR-Cas9 system
The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity...
Citation: BMC Biotechnology 2019 19:71 -
Use of the kojA promoter, involved in kojic acid biosynthesis, for polyketide production in Aspergillus oryzae: implications for long-term production
Aspergillus oryzae, a useful industrial filamentous fungus, produces limited varieties of secondary metabolites, such as kojic acid. Thus, for the production of valuable secondary metabolites by genetic engineeri...
Citation: BMC Biotechnology 2019 19:70 -
Rapid high throughput template preparation (rHTTP) method: a novel cost effective method of direct PCR for a wide range of plants
Conventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the resear...
Citation: BMC Biotechnology 2019 19:69 -
Standardized mounting method of (zebrafish) embryos using a 3D-printed stamp for high-content, semi-automated confocal imaging
Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the ze...
Citation: BMC Biotechnology 2019 19:68 -
Development of a novel human phage display-derived anti-LAG3 scFv antibody targeting CD8+ T lymphocyte exhaustion
Lymphocyte-activation gene (LAG)3 is a 498 aa transmembrane type I protein acting as an immune inhibitory receptor. It is expressed on activated lymphocytes, natural killer cells and plasmacytoid dendritic cel...
Citation: BMC Biotechnology 2019 19:67 -
Soybean plants expressing the Bacillus thuringiensis cry8-like gene show resistance to Holotrichia parallela
Cry8-like from Bacillus thuringiensis (Bt) encodes an insecticidal crystal (Cry) protein. Holotrichia parallela (Coleoptera: Scarabaeoidae), commonly known as the dark black chafer, is a troublesome pest of soybe...
Citation: BMC Biotechnology 2019 19:66 -
Rejuvenation of chicory and lettuce plants following phase change in tissue culture
A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated pla...
Citation: BMC Biotechnology 2019 19:65 -
Intracellular human antibody fragments recognizing the VP35 protein of Zaire Ebola filovirus inhibit the protein activity
Ebola hemorrhagic fever is caused by the Ebola filovirus (EBOV), which is one of the most aggressive infectious agents known worldwide. The EBOV pathogenesis starts with uncontrolled viral replication and subv...
Citation: BMC Biotechnology 2019 19:64 -
Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4
In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to...
Citation: BMC Biotechnology 2019 19:63 -
A new primer construction technique that effectively increases amplification of rare mutant templates in samples
In personalized medicine, companion diagnostic tests provide additional information to help select a treatment option likely to be optimal for a patient. Although such tests include several techniques for dete...
Citation: BMC Biotechnology 2019 19:62 -
A novel starch-binding laccase from the wheat pathogen Zymoseptoria tritici highlights the functional diversity of ascomycete laccases
Laccases are multicopper oxidases, which are assigned into auxiliary activity family 1 (AA1) in the CAZy database. These enzymes, catalyzing the oxidation of phenolic and nonphenolic substrates coupled to redu...
Citation: BMC Biotechnology 2019 19:61 -
Characterization of tethered equine chorionic gonadotropin and its deglycosylated mutants by ovulation stimulation in mice
To directly assess the biological role of oligosaccharides in recombinant equine chorionic gonadotropin (rec-eCG) functioning, cDNA encoding the full-length eCGβ-subunit was fused with the mature protein part ...
Citation: BMC Biotechnology 2019 19:60 -
Structure-based engineering of heparinase I with improved specific activity for degrading heparin
Heparinase I from Pedobacter heparinus (Ph-HepI), which specifically cleaves heparin and heparan sulfate, is one of the most extensively studied glycosaminoglycan lyases. Enzymatic degradation of heparin by hepar...
Citation: BMC Biotechnology 2019 19:59 -
Metabolic flux analysis for metabolome data validation of naturally xylose-fermenting yeasts
Efficient xylose fermentation still demands knowledge regarding xylose catabolism. In this study, metabolic flux analysis (MFA) and metabolomics were used to improve our understanding of xylose metabolism. Thu...
Citation: BMC Biotechnology 2019 19:58 -
Syneresis investigations of lacto-fermented sodium caseinate in a mixed model system
The textural characteristics of fermented dairy products are important quality parameters that play a major role in their stability and consumer’s acceptance. The aim of this study was to investigate the influ...
Citation: BMC Biotechnology 2019 19:57 -
Optimization of a nicotine degrading enzyme for potential use in treatment of nicotine addiction
Smoking and tobacco use continue to be the largest preventable causes of death globally. A novel therapeutic approach has recently been proposed: administration of an enzyme that degrades nicotine, the main ad...
Citation: BMC Biotechnology 2019 19:56 -
Lack of specificity associated with using molecular beacons in loop mediated amplification assays
Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers ...
Citation: BMC Biotechnology 2019 19:55 -
Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris
The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme a...
Citation: BMC Biotechnology 2019 19:54 -
Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO2
Increasing CO2 emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of mari...
Citation: BMC Biotechnology 2019 19:53 -
Enhancement of using combined packing materials on the removal of mixed sulfur compounds in a biotrickling filter and analysis of microbial communities
Packing materials is a critical design consideration when employing biological reactor to treat malodorous gases. The acidification of packing bed usually results in a significant drop in the removal efficienc...
Citation: BMC Biotechnology 2019 19:52 -
Mutagenesis of N-terminal residues confer thermostability on a Penicillium janthinellum MA21601 xylanase
A mesophilic xylanase PjxA from Penicillium janthinellum MA21601 has high specific activity under acidic condition and holds great potential for applications in the animal feed industry. To enhance the thermostab...
Citation: BMC Biotechnology 2019 19:51 -
Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length ...
Citation: BMC Biotechnology 2019 19:50 -
Highly efficient preparation of active S-phenyl-L-cysteine with tryptophan synthase using a chemoenzymatic method
S-Phenyl-L-cysteine is regarded as having potential applicability as an antiretroviral/protease inhibitor for human immunodeficiency virus (HIV). In the present study, optically active S-phenyl-L-cysteine was pre...
Citation: BMC Biotechnology 2019 19:49 -
Crosslinked flagella as a stabilized vaccine adjuvant scaffold
Engineered vaccine proteins incorporating both antigen and adjuvant components are constructed with the aim of combining functions to induce effective protective immunity. Bacterial flagellin is a strong candi...
Citation: BMC Biotechnology 2019 19:48 -
Preparation of a novel monoclonal antibody against caprine interleukin-17A and its applications in immunofluorescence and immunohistochemistry assays
Interleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense and many inflammatory or autoimmune diseases. The aim of this study wa...
Citation: BMC Biotechnology 2019 19:47 -
Enhanced Natamycin production by Streptomyces natalensis in shake-flasks and stirred tank bioreactor under batch and fed-batch conditions
Natamycin is an antifungal polyene macrolide antibiotic with wide applications in health and food industries. Currently, it is the only antifungal food additive with the GRAS status (Generally Regarded as Safe).
Citation: BMC Biotechnology 2019 19:46 -
Production of alkaline pectinase: a case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis CAS-MEI-2-33
Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environ...
Citation: BMC Biotechnology 2019 19:45 -
Modulation of chimeric antigen receptor surface expression by a small molecule switch
Engineered therapeutic cells have attracted a great deal of interest due to their potential applications in treating a wide range of diseases, including cancer and autoimmunity. Chimeric antigen receptor (CAR)...
Citation: BMC Biotechnology 2019 19:44 -
Purification and biochemical characterization of a novel thermostable protease from the oyster mushroom Pleurotus sajor-caju strain CTM10057 with industrial interest
Proteases are hydrolytic enzymes that catalyze peptide linkage cleavage reactions at the level of proteins and peptides with different degrees of specificity. This group draws the attention of industry. More t...
Citation: BMC Biotechnology 2019 19:43 -
Primary allogeneic mitochondrial mix (PAMM) transfer/transplant by MitoCeption to address damage in PBMCs caused by ultraviolet radiation
Artificial Mitochondrial Transfer or Transplant (AMT/T) can be used to reduce the stress and loss of viability of damaged cells. In MitoCeption, a type of AMT/T, the isolated mitochondria and recipient cells a...
Citation: BMC Biotechnology 2019 19:42 -
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very compe...
Citation: BMC Biotechnology 2019 19:41 -
VARSCOT: variant-aware detection and scoring enables sensitive and personalized off-target detection for CRISPR-Cas9
Natural variations in a genome can drastically alter the CRISPR-Cas9 off-target landscape by creating or removing sites. Despite the resulting potential side-effects from such unaccounted for sites, current of...
Citation: BMC Biotechnology 2019 19:40 -
Genome-wide response on phytosterol in 9-hydroxyandrostenedione-producing strain of Mycobacterium sp. VKM Ac-1817D
Aerobic side chain degradation of phytosterols by actinobacteria is the basis for the industrial production of androstane steroids which are the starting materials for the synthesis of steroid hormones. A nati...
Citation: BMC Biotechnology 2019 19:39 -
Mucosal delivery of Lactococcus lactis carrying an anti-TNF scFv expression vector ameliorates experimental colitis in mice
Anti-Tumor Necrosis Factor-alpha therapy has become clinically important for treating inflammatory bowel disease. However, the use of conventional immunotherapy requires a systemic exposure of patients and col...
Citation: BMC Biotechnology 2019 19:38 -
Engineered Pichia pastoris production of fusaruside, a selective immunomodulator
Fusaruside is an immunomodulatory fungal sphingolipid which has medical potentials for treating colitis and liver injury, but its poor natural abundance limits its further study.
Citation: BMC Biotechnology 2019 19:37 -
Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts
CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly cont...
Citation: BMC Biotechnology 2019 19:36 -
New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines
Viral vectors are invaluable tools to transfer genes and/or regulatory sequences into differentiated cells such as pancreatic cells. To date, several kinds of viral vectors have been used to transduce differen...
Citation: BMC Biotechnology 2019 19:35
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