Skip to main content

Articles

Page 5 of 31

  1. Consolidated bioprocessing (CBP) is a cost-effective approach for the conversion of lignocellulosic biomass to biofuels and biochemicals. The enzymatic conversion of cellulose to glucose requires the synergist...

    Authors: Martinus J. A. Daas, Bart Nijsse, Antonius H. P. van de Weijer, Bart W. A. J. Groenendaal, Fons Janssen, John van der Oost and Richard van Kranenburg

    Citation: BMC Biotechnology 2018 18:42

    Content type: Research article

    Published on:

  2. Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences of interest, including their transgene flanking regions and the unnatural assoc...

    Authors: Marie-Alice Fraiture, Julie Vandamme, Philippe Herman and Nancy H. C. Roosens

    Citation: BMC Biotechnology 2018 18:40

    Content type: Research article

    Published on:

  3. Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH)...

    Authors: Yasuhiro Watanabe, Takashi Matsuba, Mami Nakanishi, Mio Une, Ritsuko Hanajima and Kenji Nakashima

    Citation: BMC Biotechnology 2018 18:39

    Content type: Research article

    Published on:

  4. The advent of –omics technologies has enabled the resolution of fine molecular differences among individuals within a species. DNA sequence variations, such as single nucleotide polymorphisms or small deletion...

    Authors: Jean-Michel Michno and Robert M. Stupar

    Citation: BMC Biotechnology 2018 18:38

    Content type: Research article

    Published on:

  5. Custom synthesized DNA is in high demand for synthetic biology applications. However, current technologies to produce these sequences using assembly from DNA oligonucleotides are costly and labor-intensive. Th...

    Authors: Yuliya Khilko, Philip D. Weyman, John I. Glass, Mark D. Adams, Melanie A. McNeil and Peter B. Griffin

    Citation: BMC Biotechnology 2018 18:37

    Content type: Methodology article

    Published on:

  6. Phytase supplied in feeds for monogastric animals is important for improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability to...

    Authors: Nanyu Han, Huabiao Miao, Tingting Yu, Bo Xu, Yunjuan Yang, Qian Wu, Rui Zhang and Zunxi Huang

    Citation: BMC Biotechnology 2018 18:36

    Content type: Research article

    Published on:

  7. Cellulases are of great significance for full utilization of lignocellulosic biomass. Termites have an efficient ability to degrade cellulose. Heterologous production of the termite-origin cellulases is the fi...

    Authors: Pengfei Zhang, Xianghua Yuan, Yuguang Du and Jian-Jun Li

    Citation: BMC Biotechnology 2018 18:35

    Content type: Research article

    Published on:

  9. High level of serum fibroblast growth factor 23 (FGF23) is implicated in the development and progression of diabetic nephropathy (DN), making it a crucial factor in the pathogenesis of DN. FGF23 is also tightl...

    Authors: Xiaomin Zhang, Kaiwen Guo, Feng Xia, Xinyu Zhao, Zhifeng Huang and Jianlou Niu

    Citation: BMC Biotechnology 2018 18:33

    Content type: Research article

    Published on:

  10. Solvate ionic liquids (SILs) are a new class of ionic liquids that are equimolar solutions of lithium bistrifluoromethanesulfonimide in either triglyme or tetraglyme, referred to as G3LiTFSA and G4LiTFSA, resp...

    Authors: Prusothman Yoganantharajah, Alexander P. Ray, Daniel J. Eyckens, Luke C. Henderson and Yann Gibert

    Citation: BMC Biotechnology 2018 18:32

    Content type: Research article

    Published on:

  11. According to recent studies, the phospholipase A2 receptor 1 (PLA2R1) may be used as a biomarker to diagnose idiopathic membranous nephropathy (iMN). Moreover, the immune-dominant regions of PLA2R1 have been iden...

    Authors: Hua Li, Yonghua Xu, Weiming Yang and Xiaohong Huang

    Citation: BMC Biotechnology 2018 18:31

    Content type: Methodology article

    Published on:

  12. Celiac disease (CD) is one of the most common food-related chronic disorders. It is mediated by the dietary consumption of prolamins, which are storage proteins of different grains. So far, no therapy exists a...

    Authors: Britta Eggenreich, Elke Scholz, David Johannes Wurm, Florian Forster and Oliver Spadiut

    Citation: BMC Biotechnology 2018 18:30

    Content type: Research article

    Published on:

  13. β-D-xylosidase is a vital exoglycosidase with the ability to hydrolyze xylooligosaccharides to xylose and to biotransform some saponins by cleaving outer β-xylose. β-D-xylosidase is widely used as one of the x...

    Authors: Qi Li, Tao Wu, Zhipeng Qi, Linguo Zhao, Jianjun Pei and Feng Tang

    Citation: BMC Biotechnology 2018 18:29

    Content type: Research article

    Published on:

  14. Nonsense mutations promote premature translational termination, introducing stop codons within the coding region of mRNAs and causing inherited diseases, including thalassemia. For instance, in β039 thalassemia t...

    Authors: Francesca Salvatori, Mariangela Pappadà, Giulia Breveglieri, Elisabetta D’Aversa, Alessia Finotti, Ilaria Lampronti, Roberto Gambari and Monica Borgatti

    Citation: BMC Biotechnology 2018 18:28

    Content type: Research article

    Published on:

  15. As a very important coenzyme in the cell metabolism, Vitamin B12 (cobalamin, VB12) has been widely used in food and medicine fields. The complete biosynthesis of VB12 requires approximately 30 genes, but overexpr...

    Authors: Yingying Cai, Miaomiao Xia, Huina Dong, Yuan Qian, Tongcun Zhang, Beiwei Zhu, Jinchuan Wu and Dawei Zhang

    Citation: BMC Biotechnology 2018 18:27

    Content type: Research article

    Published on:

  16. Dihydro-β-ionone is a principal aroma compound and has received considerable attention by flavor and fragrance industry. The traditional method of preparing dihydro-β-ionone has many drawbacks, which has restr...

    Authors: Xuesong Zhang, Shiyong Liao, Fuliang Cao, Linguo Zhao, Jianjun Pei and Feng Tang

    Citation: BMC Biotechnology 2018 18:26

    Content type: Research article

    Published on:

  17. Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive ...

    Authors: Danping Zhang, Chengjie Xie, Rongzhi Wang, Qinghai Yang, Huiling Chen, Sumei Ling, Shihua Wang and Kunzhi Jia

    Citation: BMC Biotechnology 2018 18:25

    Content type: Research article

    Published on:

  18. The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and in...

    Authors: Xiaoyue Zhang, Keyan Xu, Yanmei Ou, Xiaodong Xu and Hongying Chen

    Citation: BMC Biotechnology 2018 18:24

    Content type: Research article

    Published on:

  19. Arachidonic acid (ARA), which is a ω-6 polyunsaturated fatty acid, has a wide range of biological activities and is an essential component of cellular membranes in some human tissues. Mortierella alpina is the be...

    Authors: Huidan Zhang, Dong Lu, Xin Li, Yingang Feng, Qiu Cui and Xiaojin Song

    Citation: BMC Biotechnology 2018 18:23

    Content type: Research article

    Published on:

  20. Violacein is a deep violet compound that is produced by a number of bacterial species. It is synthesized from tryptophan by a pathway that involves the sequential action of 5 different enzymes (encoded by genes v...

    Authors: Elizabeth Bilsland, Tatyana A. Tavella, Renata Krogh, Jamie E. Stokes, Annabelle Roberts, James Ajioka, David R. Spring, Adriano D. Andricopulo, Fabio T. M. Costa and Stephen G. Oliver

    Citation: BMC Biotechnology 2018 18:22

    Content type: Research article

    Published on:

  21. Hirudin is a potent thrombin inhibitor but its antithrombotic properties are offset by bleeding side-effects. Because hirudin’s N-terminus must engage thrombin’s active site for effective inhibition, fusing a ...

    Authors: William P. Sheffield, Louise J. Eltringham-Smith and Varsha Bhakta

    Citation: BMC Biotechnology 2018 18:21

    Content type: Research article

    Published on:

  22. Recombinant production of amebic cysteine proteases using Escherichia coli cells as the bacterial system has become a challenging effort, with protein insolubility being the most common issue. Since many of these...

    Authors: Ekaterina Jalomo-Khayrova, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Ignacio A. Rivero and Marco A. Ramos

    Citation: BMC Biotechnology 2018 18:20

    Content type: Methodology article

    Published on:

  23. Recent progress in development of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal...

    Authors: Tomoe Kobayashi, Masumi Namba, Takayuki Koyano, Masaki Fukushima, Masahiro Sato, Masato Ohtsuka and Makoto Matsuyama

    Citation: BMC Biotechnology 2018 18:19

    Content type: Research article

    Published on:

  24. Thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and could be potentially employed as bioremediation tools and bioscavengers. But nowadays their manufacturing in high...

    Authors: Odile Francesca Restaino, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Luigi Fedele, Alberto Alfano, Elena Porzio, Giuseppe Manco, Mario De Rosa and Chiara Schiraldi

    Citation: BMC Biotechnology 2018 18:18

    Content type: Research article

    Published on:

  25. We demonstrate the feasibility of creating a pair of reference samples to be used as surrogates for clinical samples measured in either a research or clinical laboratory setting. The reference sample paradigm ...

    Authors: P. Scott Pine, Steven P. Lund, Sanford A. Stass, Debra Kukuruga, Feng Jiang, Lynn Sorbara, Sudhir Srivastava and Marc Salit

    Citation: BMC Biotechnology 2018 18:17

    Content type: Methodology article

    Published on:

  26. microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, ...

    Authors: Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson and Peter J. Leedman

    Citation: BMC Biotechnology 2018 18:16

    Content type: Research article

    Published on:

  27. Preclinical studies have evaluated haptoglobin (Hp) polymers from pooled human plasma as a therapeutic protein to attenuate toxic effects of cell-free hemoglobin (Hb). Proof of concept studies have demonstrate...

    Authors: Christian A. Schaer, Catherine Owczarek, Jeremy W. Deuel, Stefan Schauer, Jin Hyen Baek, Ayla Yalamanoglu, Matthew P. Hardy, Pierre D. Scotney, Peter M. Schmidt, Matthias Pelzing, Peter Soupourmas, Paul W. Buehler and Dominik J. Schaer

    Citation: BMC Biotechnology 2018 18:15

    Content type: Research article

    Published on:

  28. Dedifferentiation of chondrocytes during cell expansion is one of the barriers in tissue construction for cartilage repair. To understand chondrocyte behavior and improve cell expansion in monolayer culture, t...

    Authors: Sopita Wongin, Saranatra Waikakul, Pojchong Chotiyarnwong, Wanwipa Siriwatwechakul, Masahiro Kino-oka, Mee-Hae Kim and Kwanchanok Viravaidya-Pasuwat

    Citation: BMC Biotechnology 2018 18:14

    Content type: Research article

    Published on:

  29. Filamentous bacteria of the genus Streptomyces produce a large arsenal of industrially relevant antibiotics and enzymes. The industrial production of these molecules occurs in large fermenters, where many strepto...

    Authors: Boris Zacchetti, Agathoklis Andrianos, Dino van Dissel, Evelien de Ruiter, Gilles P. van Wezel and Dennis Claessen

    Citation: BMC Biotechnology 2018 18:13

    Content type: Research article

    Published on:

  30. Safe and effective hemostatic materials are important for reducing mortality resulting from excessive hemorrhage. In this work, new biomaterials with hemostatic effects were created by fusing the gene coding f...

    Authors: Shasha Yang, Sili Wei, Yun Mao, Hanxue Zheng, Juantao Feng, Jihong Cui, Xin Xie, Fulin Chen and Honmgmin Li

    Citation: BMC Biotechnology 2018 18:12

    Content type: Research article

    Published on:

  31. Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these studies is the low level and instability of expression o...

    Authors: Paola Mini, Olivia Costantina Demurtas, Silvia Valentini, Patrizia Pallara, Giuseppe Aprea, Paola Ferrante and Giovanni Giuliano

    Citation: BMC Biotechnology 2018 18:11

    Content type: Research article

    Published on:

  32. The recently developed genetically encoded calcium indicator (GECI), called NTnC, has a novel design with reduced size due to utilization of the troponin C (TnC) as a Ca2+-binding moiety inserted into the mNeonGr...

    Authors: D. A. Doronin, N. V. Barykina, O. M. Subach, V. P. Sotskov, V. V. Plusnin, O. A. Ivleva, E. A. Isaakova, A. M. Varizhuk, G. E. Pozmogova, A. Y. Malyshev, I. V. Smirnov, K. D. Piatkevich, K. V. Anokhin, G. N. Enikolopov and F. V. Subach

    Citation: BMC Biotechnology 2018 18:10

    Content type: Research article

    Published on:

  33. The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and...

    Authors: Anil S. Prajapati, Vishakha A. Pawar, Ketankumar J. Panchal, Ankit P. Sudhir, Bhaumik R. Dave, Darshan H. Patel and R. B. Subramanian

    Citation: BMC Biotechnology 2018 18:9

    Content type: Research article

    Published on:

  34. Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioi...

    Authors: Christoph Geisler

    Citation: BMC Biotechnology 2018 18:8

    Content type: Methodology article

    Published on:

  35. Detection of DNA methylome at single-base resolution is a significant challenge but promises to shed considerable light on human disease etiology. Current technologies could not detect DNA methylation genome-w...

    Authors: Zhen Jia, Yueyi Shi, Lei Zhang, Yipeng Ren, Tong Wang, Lejun Xing, Baorong Zhang, Guolan Gao and Rongfa Bu

    Citation: BMC Biotechnology 2018 18:7

    Content type: Methodology article

    Published on:

    The Retraction Note to this article has been published in BMC Biotechnology 2020 20:23

  36. Circulating microRNAs are undergoing exploratory use as safety biomarkers in drug development. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is one common approach used to quantitate l...

    Authors: Russell D. Wolfinger, Sudheer Beedanagari, Eric Boitier, Tao Chen, Philippe Couttet, Heidrun Ellinger-Ziegelbauer, Gregory Guillemain, Claire Mariet, Peter Mouritzen, Raegan O’Lone, P. Scott Pine, Tatiana Sharapova, Jian Yan, Peter S. Yuen and Karol L. Thompson

    Citation: BMC Biotechnology 2018 18:6

    Content type: Methodology article

    Published on:

  37. L-phenylalanine (L-Phe) is an essential amino acid for mammals and applications expand into human health and nutritional products. In this study, a system level engineering was conducted to enhance L-Phe biosy...

    Authors: Yongfei Liu, Yiran Xu, Dongqin Ding, Jianping Wen, Beiwei Zhu and Dawei Zhang

    Citation: BMC Biotechnology 2018 18:5

    Content type: Research article

    Published on:

  38. Effective gene-delivery systems for primary human T cell engineering are useful tools for both basic research and clinical immunotherapy applications. Pseudovirus-based systems and electro-transfection are the...

    Authors: Zhang Zhang, Shunfang Qiu, Xiaopeng Zhang and Wei Chen

    Citation: BMC Biotechnology 2018 18:4

    Content type: Methodology article

    Published on:

  39. RNA interference (RNAi) technology has been widely used to knockdown target genes via post-transcriptional silencing. In plants, RNAi is used as an effective tool with diverse applications being developed such...

    Authors: Po-Hao Wang, Greg Schulenberg, Shannon Whitlock, Andrew Worden, Ning Zhou, Stephen Novak and Wei Chen

    Citation: BMC Biotechnology 2018 18:3

    Content type: Methodology article

    Published on:

  40. The concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce ...

    Authors: Agnieszka J. Turlo, Yalda Ashraf Kharaz, Peter D. Clegg, James Anderson and Mandy J. Peffers

    Citation: BMC Biotechnology 2018 18:2

    Content type: Research article

    Published on:

  41. The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) fo...

    Authors: Gerlinde Doenz, Sebastian Dorn, Narges Aghaallaei, Baubak Bajoghli, Elisabeth Riegel, Michaela Aigner, Holger Bock, Birgit Werner, Thomas Lindhorst and Thomas Czerny

    Citation: BMC Biotechnology 2018 18:1

    Content type: Research article

    Published on:

  42. Massively parallel genetic sequencing allows rapid testing of known intellectual disability (ID) genes. However, the discovery of novel syndromic ID genes requires molecular confirmation in at least a second o...

    Authors: Tracy Dudding-Byth, Anne Baxter, Elizabeth G. Holliday, Anna Hackett, Sheridan O’Donnell, Susan M. White, John Attia, Han Brunner, Bert de Vries, David Koolen, Tjitske Kleefstra, Seshika Ratwatte, Carlos Riveros, Steve Brain and Brian C. Lovell

    Citation: BMC Biotechnology 2017 17:90

    Content type: Research article

    Published on:

  43. Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning meth...

    Authors: Jacob J. Messner, Honor L. Glenn and Deirdre R. Meldrum

    Citation: BMC Biotechnology 2017 17:89

    Content type: Methodology article

    Published on:

  44. Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products depending on the regioselectivity of the immobilized lipase biocatalyst

    Authors: Erick Abreu Silveira, Sonia Moreno-Perez, Alessandra Basso, Simona Serban, Rita Pestana Mamede, Paulo W. Tardioli, Cristiane Sanchez Farinas, Javier Rocha-Martin, Gloria Fernandez-Lorente and Jose M. Guisan

    Citation: BMC Biotechnology 2017 17:88

    Content type: Research article

    Published on:

  45. Receptor activator of NF-κB ligand (RANKL)/RANK signaling essentially functions within the skeletal system, particularly participating in osteoclastogenesis and bone resorption. In addition, this signaling pat...

    Authors: Yilei Wei, Yu Zhan, Pengfei Chen, Zhi Liu, Haohao Zhang, Dandan Liu, Jie Zhang, Min Yu, Wei Mo, Jun Zhang and Xiaoren Zhang

    Citation: BMC Biotechnology 2017 17:87

    Content type: Research article

    Published on:

  46. Starch-binding domains from carbohydrate binding module family 20 have been used as a tool for starch engineering. Previous studies showed that

    Authors: Xing-Feng Huang, Farhad Nazarian, Jean-Paul Vincken, Richard G. F. Visser and Luisa M. Trindade

    Citation: BMC Biotechnology 2017 17:86

    Content type: Research article

    Published on:

  47. When the unavoidable waste generation is considered as damaging to our environment, it becomes crucial to develop a sustainable technology to remediate the pollutant source towards an environmental protection ...

    Authors: Arezoo Dadrasnia, Mohd Sofian Azirun and Salmah Binti Ismail

    Citation: BMC Biotechnology 2017 17:85

    Content type: Research article

    Published on:

  48. The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluat...

    Authors: Zhen Chen, Zhipeng Li, Peize Liu, Yu Liu, Yuanpeng Wang, Qingbiao Li and Ning He

    Citation: BMC Biotechnology 2017 17:84

    Content type: Research article

    Published on:

  50. Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO4]−, [Cl]−, [MeCO2]−) were...

    Authors: Zhao Wang, John Gräsvik, Leif J. Jönsson and Sandra Winestrand

    Citation: BMC Biotechnology 2017 17:82

    Content type: Research article

    Published on:

Submit manuscript

Follow

2019 Journal Metrics

BMC Biotechnology

ISSN: 1472-6750

Contact us