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  1. Content type: Research article

    Foot-and-mouth disease (FMD) is an acute, highly contagious disease that infects cloven-hoofed animals. Vaccination is an effective means of preventing and controlling FMD. Compared to conventional inactivated...

    Authors: Yan Xiao, Hong-Ying Chen, Yuzhou Wang, Bo Yin, Chaochao Lv, Xiaobing Mo, He Yan, Yajie Xuan, Yuxin Huang, Wenqiang Pang, Xiangdong Li, Y. Adam Yuan and Kegong Tian

    Citation: BMC Biotechnology 2016 16:56

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  2. Content type: Research article

    L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. In previous reports, AIs from thermophilic bacterial strains had been wildly researched, but the brownin...

    Authors: Wending Mei, Lu Wang, Ying Zang, Zhaojuan Zheng and Jia Ouyang

    Citation: BMC Biotechnology 2016 16:55

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  3. Content type: Research article

    Succinate is a kind of industrially important C4 platform chemical for synthesis of high value added products. Due to the economical and environmental advantages, considerable efforts on metabolic engineering ...

    Authors: Ying Zhao, Chang-Song Wang, Fei-Fei Li, Zhen-Ning Liu and Guang-Rong Zhao

    Citation: BMC Biotechnology 2016 16:52

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  4. Content type: Methodology article

    Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid trans...

    Authors: Kalpana Nanjareddy, Manoj-Kumar Arthikala, Lourdes Blanco, Elizabeth S. Arellano and Miguel Lara

    Citation: BMC Biotechnology 2016 16:53

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  5. Content type: Methodology article

    Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appro...

    Authors: P. Scott Pine, Sarah A. Munro, Jerod R. Parsons, Jennifer McDaniel, Anne Bergstrom Lucas, Jean Lozach, Timothy G. Myers, Qin Su, Sarah M. Jacobs-Helber and Marc Salit

    Citation: BMC Biotechnology 2016 16:54

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  6. Content type: Research article

    Dioxins are one of the most toxic groups of persistent organic pollutants. Their bioaccumulation through the food chain constitutes a potential risk for human health. Upon cell entry, dioxins bind specifically...

    Authors: Walaa Faiad, Abdulsamie Hanano, Mohamed Maher Kabakibi and Abdul Qader Abbady

    Citation: BMC Biotechnology 2016 16:51

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  7. Content type: Research article

    Four antigenically distinct serotypes (1–4) of dengue viruses (DENVs) cause dengue disease. Antibodies to any one DENV serotype have the potential to predispose an individual to more severe disease upon infect...

    Authors: Ankur Poddar, Viswanathan Ramasamy, Rahul Shukla, Ravi Kant Rajpoot, Upasana Arora, Swatantra K. Jain, Sathyamangalam Swaminathan and Navin Khanna

    Citation: BMC Biotechnology 2016 16:50

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  8. Content type: Research article

    Microalgae have been recognized as a good food source of natural biologically active ingredients. Among them, the green microalga Euglena is a very promising food and nutritional supplements, providing high value...

    Authors: Min Zeng, Wenlong Hao, Yongdong Zou, Mengliang Shi, Yongguang Jiang, Peng Xiao, Anping Lei, Zhangli Hu, Weiwen Zhang, Liqing Zhao and Jiangxin Wang

    Citation: BMC Biotechnology 2016 16:49

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  9. Content type: Research article

    As the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production. Cassava residues (CR) are byproducts of the cassava starch industry ...

    Authors: Li Ji, Tianran Zheng, Pengxiang Zhao, Weiming Zhang and Jianxin Jiang

    Citation: BMC Biotechnology 2016 16:48

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  10. Content type: Research article

    The ATP-binding cassette (ABC) transporter gene superfamily is ubiquitous among extant organisms and prominently represented in plants. ABC transporters act to transport compounds across cellular membranes and...

    Authors: Thomas S. Lane, Caroline S. Rempe, Jack Davitt, Margaret E. Staton, Yanhui Peng, Douglas Edward Soltis, Michael Melkonian, Michael Deyholos, James H. Leebens-Mack, Mark Chase, Carl J. Rothfels, Dennis Stevenson, Sean W. Graham, Jun Yu, Tao Liu, J. Chris Pires…

    Citation: BMC Biotechnology 2016 16:47

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  11. Content type: Research article

    Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention a...

    Authors: Yanli Sun, Yanhua Sun, Ronglan Zhao and Kunshan Gao

    Citation: BMC Biotechnology 2016 16:46

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  12. Content type: Research article

    Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass...

    Authors: Dennis Lamers, Nick van Biezen, Dirk Martens, Linda Peters, Eric van de Zilver, Nicole Jacobs-van Dreumel, René H. Wijffels and Christien Lokman

    Citation: BMC Biotechnology 2016 16:45

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  13. Content type: Research article

    Tissue engineering and bone substitutes are subjects of intensive ongoing research. If the healing of bone fractures is delayed, osteoinductive materials that induce mesenchymal stem cells (MSCs) to form bone ...

    Authors: Steffen Kissling, Michael Seidenstuecker, Ingo H. Pilz, Norbert P. Suedkamp, Hermann O. Mayr and Anke Bernstein

    Citation: BMC Biotechnology 2016 16:44

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  14. Content type: Research article

    In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the β-actin gene. This high level of the gene expression has attracted our atten...

    Authors: Roman A. Komakhin, Denis A. Vysotskii, Rahim R. Shukurov, Vera D. Voblikova, Vera V. Komakhina, Svetlana R. Strelnikova, Ekaterina M. Vetchinkina and Alexey V. Babakov

    Citation: BMC Biotechnology 2016 16:43

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  15. Content type: Research article

    L-(+)-tartaric acid (L-TA) is an important organic acid, which is produced from the cream of tartar or stereospecific hydrolysis of the cis-epoxysuccinate. The former method is limited by the availability of raw ...

    Authors: Jianfeng Yuan, Mianbin Wu, Jianping Lin and Lirong Yang

    Citation: BMC Biotechnology 2016 16:42

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  16. Content type: Research article

    The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will p...

    Authors: Justin E. Anderson, Jean-Michel Michno, Thomas J. Y. Kono, Adrian O. Stec, Benjamin W. Campbell, Shaun J. Curtin and Robert M. Stupar

    Citation: BMC Biotechnology 2016 16:41

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  17. Content type: Research

    NAC proteins (NAM (No apical meristem), ATAF (Arabidopsis transcription activation factor) and CUC (cup-shaped cotyledon)) are plant-specific transcription factors reported to be involved in regulating growth,...

    Authors: Hifzur Rahman, Valarmathi Ramanathan, Jagedeeshselvam Nallathambi, Sudhakar Duraialagaraja and Raveendran Muthurajan

    Citation: BMC Biotechnology 2016 16(Suppl 1):35

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    This article is part of a Supplement: Volume 16 Supplement 1

  18. Content type: Research

    The twin problem of shortage in fossil fuel and increase in environmental pollution can be partly addressed by blending of ethanol with transport fuel. Increasing the ethanol production for this purpose withou...

    Authors: Saranyah Kannuchamy, Nisha Mukund and Lilly M. Saleena

    Citation: BMC Biotechnology 2016 16(Suppl 1):34

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    This article is part of a Supplement: Volume 16 Supplement 1

  19. Content type: Research article

    The valuable role of immunotherapy in treating autoimmune diseases is increasingly recognized by those involved in the research and clinical application of new biopharmaceuticals products. However, many aspect...

    Authors: Gecilmara Salviato Pileggi, Aline Dayana Clemencio, Thiago Malardo, Sonir R Antonini, Vania Luiza Deperon Bonato, Wendy Martin Rios and Celio L Silva

    Citation: BMC Biotechnology 2016 16:40

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  20. Content type: Research article

    Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric pathogen of swine. The spike glycoprotein (S) of PEDV is the major immunogenic determinant that plays a pivotal role in the induction of ne...

    Authors: Da-Chuan Piao, Do-Woon Shin, In-Seon Kim, Hui-Shan Li, Seo-Ho Oh, Bijay Singh, S. Maharjan, Yoon-Seok Lee, Jin-Duck Bok, Chong-Su Cho, Zhong-Shan Hong, Sang-Kee Kang and Yun-Jaie Choi

    Citation: BMC Biotechnology 2016 16:39

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  21. Content type: Research article

    The whole-cell lipase from Burkholderia cepacia has been used as a biocatalyst in organic synthesis. However, there is no report in the literature on the component or the gene sequence of the cell-bound lipase fr...

    Authors: Zhengyu Shu, Hong Lin, Shaolei Shi, Xiangduo Mu, Yanru Liu and Jianzhong Huang

    Citation: BMC Biotechnology 2016 16:38

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  22. Content type: Methodology article

    Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a...

    Authors: Asuka Mukai, Aya Ichiraku and Kazuki Horikawa

    Citation: BMC Biotechnology 2016 16:37

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  23. Content type: Methodology article

    G protein-coupled receptors (GPCRs) are ubiquitous surface proteins mediating various biological responses and thus, important targets for therapeutic drugs. GPCRs individually produce their own signaling as w...

    Authors: Yuki Nonobe, Tomoki Yokoyama, Yuji Kamikubo, Sho Yoshida, Nozomi Hisajima, Hiroaki Shinohara, Yuki Shiraishi, Takashi Sakurai and Toshihide Tabata

    Citation: BMC Biotechnology 2016 16:36

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  24. Content type: Research article

    Angiotensinogen (ANG) is a macromolecular precursor of angiotensin, which regulates blood pressure and electrolyte balance. ANG is specifically cleaved by renin, an aspartic protease, to initiate the angiotens...

    Authors: Shinji Yamashita, Naoya Shibata, Akiyoshi Boku-Ikeda, Erika Abe, Ayumi Inayama, Takashi Yamaguchi, Ayano Higuma, Kaoru Inagaki, Tomoyo Tsuyuzaki, Satoshi Iwamoto, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Kazal Boron Biswas, A. H. M. Nurun Nabi, Tsutomu Nakagawa…

    Citation: BMC Biotechnology 2016 16:33

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  25. Content type: Research article

    Fetal bovine serum (FBS) contains a wide range of growth factors, hormones, vitamins, amino acids, fatty acids and trace elements required for cell growth. It was shown that animal sera contain also extracellu...

    Authors: Hala Aswad, Audrey Jalabert and Sophie Rome

    Citation: BMC Biotechnology 2016 16:32

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  26. Content type: Research article

    Vanillin, a type of phenolic released during the pre-treatment of lignocellulosic materials, is toxic to microorganisms and therefore its presence inhibits the fermentation. The vanillin can be reduced to vani...

    Authors: Xinning Wang, Zhenzhen Liang, Jin Hou, Xiaoming Bao and Yu Shen

    Citation: BMC Biotechnology 2016 16:31

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  27. Content type: Research article

    Diabetes and its concurrent complications impact a significant proportion of the population of the US and create a large financial burden on the American health care system. FDA-approved maggot debridement the...

    Authors: Rebecca J. Linger, Esther J. Belikoff, Ying Yan, Fang Li, Holly A. Wantuch, Helen L. Fitzsimons and Maxwell J. Scott

    Citation: BMC Biotechnology 2016 16:30

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  30. Content type: Methodology article

    Research into gene expression enables scientists to decipher the complex regulatory networks that control fundamental biological processes. Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method...

    Authors: Nicky Boulter, Francia Garces Suarez, Stephen Schibeci, Trevor Sunderland, Ornella Tolhurst, Tegan Hunter, George Hodge, David Handelsman, Ulla Simanainen, Edward Hendriks and Karen Duggan

    Citation: BMC Biotechnology 2016 16:27

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  31. Content type: Research article

    Hydroxy fatty acids (HFAs) are valuable chemicals for a broad variety of applications. However, commercial production of HFAs has not been established so far due to the lack of low cost routes for their synthe...

    Authors: Yujin Cao, Tao Cheng, Guang Zhao, Wei Niu, Jiantao Guo, Mo Xian and Huizhou Liu

    Citation: BMC Biotechnology 2016 16:26

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  32. Content type: Research article

    Rice sheath blight, caused by Rhizoctonia solani is one of the most devastating diseases of rice. It is associated with significant reduction in rice productivity worldwide. A mutant variant of mannose binding Al...

    Authors: Prithwi Ghosh, Senjuti Sen, Joydeep Chakraborty and Sampa Das

    Citation: BMC Biotechnology 2016 16:24

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  33. Content type: Research

    Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. However, the low natural abundance of IGF-I limits it...

    Authors: Shujie Liu, Yongming Li, Bo Deng and Ziwei Xu

    Citation: BMC Biotechnology 2016 16:25

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  34. Content type: Research article

    The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other protein) has found widespread application in basic re...

    Authors: Dona York, Jeanne Baker, Patrick G. Holder, Lesley C. Jones, Penelope M. Drake, Robyn M. Barfield, Gregory T. Bleck and David Rabuka

    Citation: BMC Biotechnology 2016 16:23

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  35. Content type: Research article

    We previously developed in vitro immunization based on a fusion protein containing the transcriptional transactivator (Tat) of human immunodeficiency virus and a double domain, called ZZ, derived from protein A o...

    Authors: A. Wijkhuisen, A. Savatier, N. Cordeiro and M. Léonetti

    Citation: BMC Biotechnology 2016 16:22

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  36. Content type: Research article

    Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here...

    Authors: Palanisamy Athiyaman Balakumaran, Jan Förster, Martin Zimmermann, Jayachandran Charumathi, Andreas Schmitz, Eik Czarnotta, Mathias Lehnen, Suresh Sudarsan, Birgitta E. Ebert, Lars Mathias Blank and Sankaranarayanan Meenakshisundaram

    Citation: BMC Biotechnology 2016 16:20

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  37. Content type: Research article

    The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from

    Authors: Weifeng Sun, Longbao Zhu, Xianggui Chen, Ping Chen, Lingling Yang, Wenwu Ding, Zhemin Zhou and Yi Liu

    Citation: BMC Biotechnology 2016 16:21

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  38. Content type: Research article

    A thermal tolerant stereo-complex poly-lactic acid (SC-PLA) can be made by mixing Poly-D-lactic acid (PDLA) and poly-L-lactic acid (PLLA) at a defined ratio. This environmentally friendly biodegradable polymer...

    Authors: Hongying Lu, Xiao Zhao, Yongze Wang, Xiaoren Ding, Jinhua Wang, Erin Garza, Ryan Manow, Andrew Iverson and Shengde Zhou

    Citation: BMC Biotechnology 2016 16:19

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  39. Content type: Research article

    In an earlier study we developed a unique strategy allowing us to specifically eliminate antigen-specific murine B cells via their distinct B cell receptors using a new class of fusion proteins. In the present...

    Authors: Diana Klose, Ute Saunders, Stefan Barth, Rainer Fischer, Annett Marita Jacobi and Thomas Nachreiner

    Citation: BMC Biotechnology 2016 16:18

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  40. Content type: Research article

    The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requi...

    Authors: C. Liesche, L. Venkatraman, S. Aschenbrenner, S. Grosse, D. Grimm, R. Eils and J. Beaudouin

    Citation: BMC Biotechnology 2016 16:17

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  41. Content type: Research article

    von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Intere...

    Authors: Hee Young Yoo, Young Hoon Song, Mathias Foo, Eunseok Seo, Dong Soo Hwang and Jeong Hyun Seo

    Citation: BMC Biotechnology 2016 16:16

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  43. Content type: Research article

    Nitrilases, which hydrolyze nitriles in a one-step reaction into carboxylic acids and ammonia, gained increasing attention because of the abundance of nitrile compounds in nature and their use in fine chemical...

    Authors: Sarah A. Sonbol, Ari J. S. Ferreira and Rania Siam

    Citation: BMC Biotechnology 2016 16:14

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  44. Content type: Research article

    Phage-encoded serine integrases, such as ϕC31 integrase, are widely used for genome engineering but have not been optimized for use in Saccharomyces cerevisiae although this organism is a widely used organism in ...

    Authors: Zhengyao Xu and William R. A. Brown

    Citation: BMC Biotechnology 2016 16:13

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  45. Content type: Research article

    Dengue is a viral disease spread to humans by mosquitoes. Notably, there are four serotypes of dengue viruses (DENV) that places ~40 % of the global population at risk of infection. However, lack of a suitable...

    Authors: Neha Kaushik, Deepak Rohila, Upasana Arora, Rajendra Raut, Urpo Lamminmäki, Navin Khanna and Gaurav Batra

    Citation: BMC Biotechnology 2016 16:12

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  46. Content type: Research article

    Aspergillus fumigatus R1 produced xylanase under submerged fermentation which degrades the complex hemicelluloses contained in agricultural substrates. Xylanases have gained considerab...

    Authors: Rehan Ahmed Deshmukh, Sharmili Jagtap, Madan Kumar Mandal and Suraj Kumar Mandal

    Citation: BMC Biotechnology 2016 16:11

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  47. Content type: Research article

    Mesenchymal stem cells (MSCs) are viewed as a having significant potential for tissue engineering and regenerative medicine therapies. Clinical implementation of MSCs, however, demands that their preparation b...

    Authors: Stephen M. Goldman and Gilda A. Barabino

    Citation: BMC Biotechnology 2016 16:10

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  49. Content type: Research article

    CD40, also called Bp50, is a novel member of the TNF receptor superfamily. Based on its important role in multiple physiological and pathological processes, the CD40 signaling pathway has become a vital target...

    Authors: Yu Zhan, Yilei Wei, Pengfei Chen, Haohao Zhang, Dandan Liu, Jie Zhang, Rongzeng Liu, Ran Chen, Jun Zhang, Wei Mo and Xiaoren Zhang

    Citation: BMC Biotechnology 2016 16:8

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