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  1. Content type: Research article

    Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter...

    Authors: Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R. Johnson, Micaha Hanson, Jason N. Burris, Charles Kwit and C. Neal Stewart Jr

    Citation: BMC Biotechnology 2017 17:40

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  2. Content type: Research article

    Propionate is widely used as an important preservative and important chemical intermediate for synthesis of cellulose fibers, herbicides, perfumes and pharmaceuticals. Biosynthetic propionate has mainly been p...

    Authors: Jing Li, Xinna Zhu, Jing Chen, Dongdong Zhao, Xueli Zhang and Changhao Bi

    Citation: BMC Biotechnology 2017 17:38

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  3. Content type: Research article

    Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Authors: Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    Citation: BMC Biotechnology 2017 17:37

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  4. Content type: Research article

    N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Al...

    Authors: Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge and Chieh-Chen Huang

    Citation: BMC Biotechnology 2017 17:36

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  5. Content type: Research article

    Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which t...

    Authors: Nadezhda A. Orlova, Sergey V. Kovnir, Alexandre G. Gabibov and Ivan I. Vorobiev

    Citation: BMC Biotechnology 2017 17:33

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  6. Content type: Methodology article

    Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...

    Authors: Fanli Zeng, Zhimin Hao, Pan Li, Yanan Meng, Jingao Dong and Yibin Lin

    Citation: BMC Biotechnology 2017 17:32

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  7. Content type: Research article

    Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k ...

    Authors: Qing Wang, Luyao Bao, Chenjun Jia, Mei Li, Jian-Jun Li and Xuefeng Lu

    Citation: BMC Biotechnology 2017 17:31

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  8. Content type: Methodology article

    Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have...

    Authors: Jun-Zheng Wu, Qin Liu, Xiao-Shan Geng, Kai-Mian Li, Li-Juan Luo and Jin-Ping Liu

    Citation: BMC Biotechnology 2017 17:29

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  9. Content type: Research article

    The tissue culture banana (TCB) is a biotechnological agricultural innovation that has been adopted widely in commercial banana production. In 2003, Africa Harvest Biotech Foundation International (AH) initiat...

    Authors: Sunita V. S. Bandewar, Florence Wambugu, Emma Richardson and James V. Lavery

    Citation: BMC Biotechnology 2017 17:28

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  10. Content type: Research article

    Oleaginous organisms are a promising, renewable source of single cell oil. Lipid accumulation is mainly induced by limitation of nutrients such as nitrogen, phosphorus or sulfur. The oleaginous yeast Trichosporon...

    Authors: Felix Bracharz, Veronika Redai, Kathrin Bach, Farah Qoura and Thomas Brück

    Citation: BMC Biotechnology 2017 17:27

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  11. Content type: Research article

    Xylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown.

    Authors: Yihua Zhan, Xiangyu Sun, Guozeng Rong, Chunxiao Hou, Yingying Huang, Dean Jiang and Xiaoyan Weng

    Citation: BMC Biotechnology 2017 17:26

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  12. Content type: Research article

    Recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. Escherichia coli heat-labile...

    Authors: Nan Sun, Rongguang Zhang, Guangcai Duan, Xiaoyan Peng, Chen Wang, Qingtang Fan, Shuaiyin Chen and Yuanlin Xi

    Citation: BMC Biotechnology 2017 17:25

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  13. Content type: Methodology article

    Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian...

    Authors: Sander B. Frank, Veronique V. Schulz and Cindy K. Miranti

    Citation: BMC Biotechnology 2017 17:24

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  14. Content type: Research article

    Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and b...

    Authors: Gabe Javitt, Zohar Ben-Barak-Zelas, Moran Jerabek-Willemsen and Ayelet Fishman

    Citation: BMC Biotechnology 2017 17:23

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  15. Content type: Research article

    α-Amylase plays a pivotal role in a broad range of industrial processes. To meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. Howe...

    Authors: Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, Siti Aisyah Alias, Bei Gao and Dongzhi Wei

    Citation: BMC Biotechnology 2017 17:22

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  16. Content type: Research article

    Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability...

    Authors: Lin Ge, Anna Chen, Jianjun Pei, Linguo Zhao, Xianying Fang, Gang Ding, Zhenzhong Wang, Wei Xiao and Feng Tang

    Citation: BMC Biotechnology 2017 17:21

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  17. Content type: Research article

    poly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-sca...

    Authors: Jun Xia, Jiaxing Xu, Xiaoyan Liu, Jiming Xu, Xingfeng Wang and Xiangqian Li

    Citation: BMC Biotechnology 2017 17:20

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  18. Content type: Research article

    Manganese peroxidase (MnP) of white rot basidiomycetes, an extracellular heme enzyme, is part of a peroxidase superfamily that is capable of degrading the different phenolic compounds. Ganoderma, a white rot basi...

    Authors: Hui Xu, Meng-Yuan Guo, Yan-Hua Gao, Xiao-Hui Bai and Xuan-Wei Zhou

    Citation: BMC Biotechnology 2017 17:19

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  19. Content type: Research article

    As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cel...

    Authors: Fatemeh Nematpour, Fereidoun Mahboudi, Behrouz Vaziri, Vahid Khalaj, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat and Fatemeh Davami

    Citation: BMC Biotechnology 2017 17:18

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  20. Content type: Research article

    Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Authors: Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    Citation: BMC Biotechnology 2017 17:17

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  21. Content type: Research article

    Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many pr...

    Authors: Sandra Ziemons, Katerina Koutsantas, Kordula Becker, Tim Dahlmann and Ulrich Kück

    Citation: BMC Biotechnology 2017 17:16

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  22. Content type: Research article

    Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-pol...

    Authors: Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua and Duriya Chantasingh

    Citation: BMC Biotechnology 2017 17:15

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  23. Content type: Research article

    Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Authors: Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    Citation: BMC Biotechnology 2017 17:14

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  24. Content type: Methodology article

    Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Authors: Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    Citation: BMC Biotechnology 2017 17:13

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  25. Content type: Research article

    Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As...

    Authors: Aiswarya Chenthamarakshan, Nayana Parambayil, Nafeesathul Miziriya, P. S. Soumya, M. S. Kiran Lakshmi, Anala Ramgopal, Anuja Dileep and Padma Nambisan

    Citation: BMC Biotechnology 2017 17:12

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  26. Content type: Research article

    Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Authors: Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    Citation: BMC Biotechnology 2017 17:11

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  27. Content type: Research article

    RNAi (RNA interference) is a technology for silencing of target genes via sequence-specific manner. RNAi technology has been used for development of anti-pathogenic crops. In 2007, development of transgenic plant...

    Authors: Oyunchuluun Ganbaatar, Budao Cao, Yanan Zhang, Duran Bao, Wenhua Bao and Hada Wuriyanghan

    Citation: BMC Biotechnology 2017 17:9

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  28. Content type: Research article

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Authors: Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    Citation: BMC Biotechnology 2017 17:8

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  29. Content type: Research article

    Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthet...

    Authors: Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song and Xuguo Zhou

    Citation: BMC Biotechnology 2017 17:7

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  30. Content type: Research article

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Authors: Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    Citation: BMC Biotechnology 2017 17:6

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  31. Content type: Research article

    Initially known as the reproductive hormone, relaxin was shown to possess other therapeutically useful properties that include extracellular matrix remodeling, anti-inflammatory, anti-ischemic and angiogenic e...

    Authors: D. Cimini, K. Della Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. V. A. Pirozzi, F. Ferrara, R. Formisano, M. De Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone and C. Schiraldi

    Citation: BMC Biotechnology 2017 17:4

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  32. Content type: Methodology article

    There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

    Authors: Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

    Citation: BMC Biotechnology 2017 17:3

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  33. Content type: Research article

    Human infection with avian influenza A virus (H7N9) was first reported in China in March 2013. Since then, hundreds of cases have been confirmed showing severe symptoms with a high mortality rate. The virus wa...

    Authors: Che-Ming Jack Hu, Chu-Yang Chien, Ming-Tsan Liu, Zih-Syun Fang, Sui-Yuan Chang, Rong-Huay Juang, Shih-Chung Chang and Hui-Wen Chen

    Citation: BMC Biotechnology 2017 17:2

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  34. Content type: Methodology article

    Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affi...

    Authors: Marco Biancucci, Jazel S. Dolores, Jennifer Wong, Sarah Grimshaw, Wayne F. Anderson, Karla J. F. Satchell and Keehwan Kwon

    Citation: BMC Biotechnology 2017 17:1

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  35. Content type: Research article

    The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Authors: Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    Citation: BMC Biotechnology 2016 16:89

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  36. Content type: Research article

    Manganese peroxidase (MnP) from Irpex lacteus F17 has been shown to have a strong ability to degrade recalcitrant aromatic pollutants. In this study, a recombinant MnP from I. lacteus F17 was expressed in Escheri...

    Authors: Nan Wang, Kai Ren, Rong Jia, Wenting Chen and Ruirui Sun

    Citation: BMC Biotechnology 2016 16:87

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  37. Content type: Methodology article

    Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenes...

    Authors: Ana Filipa Sequeira, Joana L. A. Brás, Catarina I. P. D. Guerreiro, Renaud Vincentelli and Carlos M. G. A. Fontes

    Citation: BMC Biotechnology 2016 16:86

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  38. Content type: Research article

    Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is ...

    Authors: Yahaira Naaldijk, Adiv A. Johnson, Annett Friedrich-Stöckigt and Alexandra Stolzing

    Citation: BMC Biotechnology 2016 16:85

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  39. Content type: Research article

    The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

    Authors: Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

    Citation: BMC Biotechnology 2016 16:84

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  40. Content type: Methodology article

    Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

    Authors: Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

    Citation: BMC Biotechnology 2016 16:83

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  41. Content type: Research article

    Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their compo...

    Authors: Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana and Verawat Champreda

    Citation: BMC Biotechnology 2016 16:82

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  42. Content type: Research article

    Tobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of...

    Authors: Yulong Su, He Xian, Sujuan Shi, Chengsheng Zhang, S. M. Nuruzzaman Manik, Jingjing Mao, Ge Zhang, Weihong Liao, Qian Wang and Haobao Liu

    Citation: BMC Biotechnology 2016 16:81

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  43. Content type: Research article

    Hexuronic acids such as D-galacturonic acid and D-glucuronic acid can be utilized via different pathways within the metabolism of microorganisms. One representative, the oxidative pathway, generates α-keto-glu...

    Authors: André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    Citation: BMC Biotechnology 2016 16:80

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