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  1. Content type: Methodology article

    There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

    Authors: Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

    Citation: BMC Biotechnology 2017 17:3

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  2. Content type: Research article

    Human infection with avian influenza A virus (H7N9) was first reported in China in March 2013. Since then, hundreds of cases have been confirmed showing severe symptoms with a high mortality rate. The virus wa...

    Authors: Che-Ming Jack Hu, Chu-Yang Chien, Ming-Tsan Liu, Zih-Syun Fang, Sui-Yuan Chang, Rong-Huay Juang, Shih-Chung Chang and Hui-Wen Chen

    Citation: BMC Biotechnology 2017 17:2

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  3. Content type: Methodology article

    Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affi...

    Authors: Marco Biancucci, Jazel S. Dolores, Jennifer Wong, Sarah Grimshaw, Wayne F. Anderson, Karla J. F. Satchell and Keehwan Kwon

    Citation: BMC Biotechnology 2017 17:1

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  4. Content type: Research article

    The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Authors: Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    Citation: BMC Biotechnology 2016 16:89

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  5. Content type: Research article

    Manganese peroxidase (MnP) from Irpex lacteus F17 has been shown to have a strong ability to degrade recalcitrant aromatic pollutants. In this study, a recombinant MnP from I. lacteus F17 was expressed in Escheri...

    Authors: Nan Wang, Kai Ren, Rong Jia, Wenting Chen and Ruirui Sun

    Citation: BMC Biotechnology 2016 16:87

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  6. Content type: Methodology article

    Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is quickly replacing the classical cloning and mutagenes...

    Authors: Ana Filipa Sequeira, Joana L. A. Brás, Catarina I. P. D. Guerreiro, Renaud Vincentelli and Carlos M. G. A. Fontes

    Citation: BMC Biotechnology 2016 16:86

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  7. Content type: Research article

    Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is ...

    Authors: Yahaira Naaldijk, Adiv A. Johnson, Annett Friedrich-Stöckigt and Alexandra Stolzing

    Citation: BMC Biotechnology 2016 16:85

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  8. Content type: Research article

    The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

    Authors: Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

    Citation: BMC Biotechnology 2016 16:84

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  9. Content type: Methodology article

    Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

    Authors: Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

    Citation: BMC Biotechnology 2016 16:83

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  10. Content type: Research article

    Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their compo...

    Authors: Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana and Verawat Champreda

    Citation: BMC Biotechnology 2016 16:82

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  11. Content type: Research article

    Tobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of...

    Authors: Yulong Su, He Xian, Sujuan Shi, Chengsheng Zhang, S. M. Nuruzzaman Manik, Jingjing Mao, Ge Zhang, Weihong Liao, Qian Wang and Haobao Liu

    Citation: BMC Biotechnology 2016 16:81

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  12. Content type: Research article

    Hexuronic acids such as D-galacturonic acid and D-glucuronic acid can be utilized via different pathways within the metabolism of microorganisms. One representative, the oxidative pathway, generates α-keto-glu...

    Authors: André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    Citation: BMC Biotechnology 2016 16:80

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  13. Content type: Research article

    Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involve...

    Authors: Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang and Tingyi Wen

    Citation: BMC Biotechnology 2016 16:79

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  14. Content type: Research article

    The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA)...

    Authors: Yanxia Zhu, Xiaomin Wu, Yuhong Liang, Hongsheng Gu, Kedong Song, Xuenong Zou and Guangqian Zhou

    Citation: BMC Biotechnology 2016 16:78

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  15. Content type: Research article

    Family 11 alkaline xylanases have great potential economic applications in the pulp and paper industry. In this study, we would improve the alkalophilicity of family 11 alkaline xylanase Xyn11A-LC from Bacillus s...

    Authors: Wenqin Bai, Yufan Cao, Jun Liu, Qinhong Wang and Zhenhu Jia

    Citation: BMC Biotechnology 2016 16:77

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  16. Content type: Methodology article

    Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of i...

    Authors: Hanna Amelina, Vera Moiseeva, Laura Catharine Collopy, Siân Rosanna Pearson, Christine Anne Armstrong and Kazunori Tomita

    Citation: BMC Biotechnology 2016 16:76

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  17. Content type: Research article

    Transglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptom...

    Authors: Song Liu, Miao Wang, Guocheng Du and Jian Chen

    Citation: BMC Biotechnology 2016 16:75

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  18. Content type: Research article

    The isolation of unknown DNA sequences flanked by known sequences is an important task in the event-specific detection of GMOs. None of event-specific detection method was developed based on the junction seque...

    Authors: Hongwei Gao, Xiaofan Yu, Tingting Deng, Min Sun, Xizhi Xiao, Xin Huang, Ying Chen and Ronggui Li

    Citation: BMC Biotechnology 2016 16:74

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  19. Content type: Research article

    Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan. Appended ...

    Authors: Shiping Liu and Shaojun Ding

    Citation: BMC Biotechnology 2016 16:73

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  20. Content type: Research article

    A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of ...

    Authors: Guojun Wang, Ting Zhang, Haifeng Huang, Shurong Hou, Xiabin Chen, Fang Zheng and Chang-Guo Zhan

    Citation: BMC Biotechnology 2016 16:72

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  21. Content type: Research article

    Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter d...

    Authors: Steven C. L. Ho, Esther Y. C. Koh, Benjamin P. C. Soo, Mariati, Sheng-Hao Chao and Yuansheng Yang

    Citation: BMC Biotechnology 2016 16:71

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  22. Content type: Research article

    Ethyl (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE) is a versatile chiral precursor for many pharmaceuticals. Although several biosynthesis strategies have been documented to convert ethyl 4-chloro-3-oxobutanoate (CO...

    Authors: Xiang Chen, Zhi-Qiang Liu, Chao-Ping Lin and Yu-Guo Zheng

    Citation: BMC Biotechnology 2016 16:70

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  23. Content type: Research article

    Abiotic stresses are serious threats to plant growth, productivity and result in crop loss worldwide, reducing average yields of most major crops. Although abiotic stresses might elicit different plant respons...

    Authors: Yuanyuan Bu, Bo Sun, Aimin Zhou, Xinxin Zhang, Testuo Takano and Shenkui Liu

    Citation: BMC Biotechnology 2016 16:69

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  24. Content type: Research article

    RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five treh...

    Authors: Bin Tang, Ping Wei, Lina Zhao, Zuokun Shi, Qida Shen, Mengmeng Yang, Guoqiang Xie and Shigui Wang

    Citation: BMC Biotechnology 2016 16:67

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  25. Content type: Methodology article

    Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is ...

    Authors: Alexander Falkenhagen, Sabah Asad, Stanley E. Read and Sadhna Joshi

    Citation: BMC Biotechnology 2016 16:66

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  26. Content type: Research article

    Targeted therapies for cancer, especially the malignant cancer, are always restricted by the deficiency of tumor-specific drug delivery methods. Subtilase cytotoxic is a virulent cytotoxin, and the subunit A (...

    Authors: Lichao Zhang, Zongwei Li, Tonglin Shi, Xiaoqin La, Hanqing Li and Zhuoyu Li

    Citation: BMC Biotechnology 2016 16:65

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  27. Content type: Methodology article

    Infectious clones are fundamental tools for the study of many viruses, allowing for efficient mutagenesis and reproducible production of genetically-defined strains. For the large dsDNA genomes of the herpesvi...

    Authors: Alexsia L. Richards, Patricia J. Sollars and Gregory A. Smith

    Citation: BMC Biotechnology 2016 16:64

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  28. Content type: Research article

    Transcription factors (TFs) are effectors of cell signaling pathways that regulate gene expression. TF networks are highly interconnected; one signal can lead to changes in many TF levels, and one TF level can...

    Authors: Betul Bilgin, Aritro Nath, Christina Chan and S. Patrick Walton

    Citation: BMC Biotechnology 2016 16:62

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  29. Content type: Research article

    Diols are important monomers for the production of plastics and polyurethanes, which are widely used in our daily life. The medium-chain diols with one hydroxyl group at its subterminal end are able to confer ...

    Authors: Yanning Zheng, Lingling Li, Qiang Liu, Haibo Zhang, Yujin Cao, Mo Xian and Huizhou Liu

    Citation: BMC Biotechnology 2016 16:61

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  30. Content type: Methodology article

    Selected microRNAs (miRNAs) that are abnormally expressed in the serum of patients with lung cancer have recently been proposed as biomarkers of this disease. The measurement of circulating miRNAs, however, re...

    Authors: Paola Campomenosi, Elisabetta Gini, Douglas M. Noonan, Albino Poli, Paola D’Antona, Nicola Rotolo, Lorenzo Dominioni and Andrea Imperatori

    Citation: BMC Biotechnology 2016 16:60

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  31. Content type: Methodology article

    CRISPR/Cas9 genome editing strategy has been applied to a variety of species and the tRNA-processing system has been used to compact multiple gRNAs into one synthetic gene for manipulating multiple genes in rice.

    Authors: Weiwei Qi, Tong Zhu, Zhongrui Tian, Chaobin Li, Wei Zhang and Rentao Song

    Citation: BMC Biotechnology 2016 16:58

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  32. Content type: Methodology article

    Large efforts have focused on screening for genes involved in the virulence and pathogenicity of Verticillium dahliae, a destructive fungal pathogen of numerous plant species that is difficult to control once the...

    Authors: Latifur Rehman, Xiaofeng Su, Huiming Guo, Xiliang Qi and Hongmei Cheng

    Citation: BMC Biotechnology 2016 16:57

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  33. Content type: Research article

    Foot-and-mouth disease (FMD) is an acute, highly contagious disease that infects cloven-hoofed animals. Vaccination is an effective means of preventing and controlling FMD. Compared to conventional inactivated...

    Authors: Yan Xiao, Hong-Ying Chen, Yuzhou Wang, Bo Yin, Chaochao Lv, Xiaobing Mo, He Yan, Yajie Xuan, Yuxin Huang, Wenqiang Pang, Xiangdong Li, Y. Adam Yuan and Kegong Tian

    Citation: BMC Biotechnology 2016 16:56

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  34. Content type: Research article

    L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. In previous reports, AIs from thermophilic bacterial strains had been wildly researched, but the brownin...

    Authors: Wending Mei, Lu Wang, Ying Zang, Zhaojuan Zheng and Jia Ouyang

    Citation: BMC Biotechnology 2016 16:55

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  35. Content type: Methodology article

    Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appro...

    Authors: P. Scott Pine, Sarah A. Munro, Jerod R. Parsons, Jennifer McDaniel, Anne Bergstrom Lucas, Jean Lozach, Timothy G. Myers, Qin Su, Sarah M. Jacobs-Helber and Marc Salit

    Citation: BMC Biotechnology 2016 16:54

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  36. Content type: Methodology article

    Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid trans...

    Authors: Kalpana Nanjareddy, Manoj-Kumar Arthikala, Lourdes Blanco, Elizabeth S. Arellano and Miguel Lara

    Citation: BMC Biotechnology 2016 16:53

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  37. Content type: Research article

    Succinate is a kind of industrially important C4 platform chemical for synthesis of high value added products. Due to the economical and environmental advantages, considerable efforts on metabolic engineering ...

    Authors: Ying Zhao, Chang-Song Wang, Fei-Fei Li, Zhen-Ning Liu and Guang-Rong Zhao

    Citation: BMC Biotechnology 2016 16:52

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  38. Content type: Research article

    Dioxins are one of the most toxic groups of persistent organic pollutants. Their bioaccumulation through the food chain constitutes a potential risk for human health. Upon cell entry, dioxins bind specifically...

    Authors: Walaa Faiad, Abdulsamie Hanano, Mohamed Maher Kabakibi and Abdul Qader Abbady

    Citation: BMC Biotechnology 2016 16:51

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  39. Content type: Research article

    Four antigenically distinct serotypes (1–4) of dengue viruses (DENVs) cause dengue disease. Antibodies to any one DENV serotype have the potential to predispose an individual to more severe disease upon infect...

    Authors: Ankur Poddar, Viswanathan Ramasamy, Rahul Shukla, Ravi Kant Rajpoot, Upasana Arora, Swatantra K. Jain, Sathyamangalam Swaminathan and Navin Khanna

    Citation: BMC Biotechnology 2016 16:50

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  40. Content type: Research article

    Microalgae have been recognized as a good food source of natural biologically active ingredients. Among them, the green microalga Euglena is a very promising food and nutritional supplements, providing high value...

    Authors: Min Zeng, Wenlong Hao, Yongdong Zou, Mengliang Shi, Yongguang Jiang, Peng Xiao, Anping Lei, Zhangli Hu, Weiwen Zhang, Liqing Zhao and Jiangxin Wang

    Citation: BMC Biotechnology 2016 16:49

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  41. Content type: Research article

    As the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production. Cassava residues (CR) are byproducts of the cassava starch industry ...

    Authors: Li Ji, Tianran Zheng, Pengxiang Zhao, Weiming Zhang and Jianxin Jiang

    Citation: BMC Biotechnology 2016 16:48

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  42. Content type: Research article

    The ATP-binding cassette (ABC) transporter gene superfamily is ubiquitous among extant organisms and prominently represented in plants. ABC transporters act to transport compounds across cellular membranes and...

    Authors: Thomas S. Lane, Caroline S. Rempe, Jack Davitt, Margaret E. Staton, Yanhui Peng, Douglas Edward Soltis, Michael Melkonian, Michael Deyholos, James H. Leebens-Mack, Mark Chase, Carl J. Rothfels, Dennis Stevenson, Sean W. Graham, Jun Yu, Tao Liu, J. Chris Pires…

    Citation: BMC Biotechnology 2016 16:47

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  43. Content type: Research article

    Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention a...

    Authors: Yanli Sun, Yanhua Sun, Ronglan Zhao and Kunshan Gao

    Citation: BMC Biotechnology 2016 16:46

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  44. Content type: Research article

    Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass...

    Authors: Dennis Lamers, Nick van Biezen, Dirk Martens, Linda Peters, Eric van de Zilver, Nicole Jacobs-van Dreumel, René H. Wijffels and Christien Lokman

    Citation: BMC Biotechnology 2016 16:45

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  45. Content type: Research article

    Tissue engineering and bone substitutes are subjects of intensive ongoing research. If the healing of bone fractures is delayed, osteoinductive materials that induce mesenchymal stem cells (MSCs) to form bone ...

    Authors: Steffen Kissling, Michael Seidenstuecker, Ingo H. Pilz, Norbert P. Suedkamp, Hermann O. Mayr and Anke Bernstein

    Citation: BMC Biotechnology 2016 16:44

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  46. Content type: Research article

    In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the β-actin gene. This high level of the gene expression has attracted our atten...

    Authors: Roman A. Komakhin, Denis A. Vysotskii, Rahim R. Shukurov, Vera D. Voblikova, Vera V. Komakhina, Svetlana R. Strelnikova, Ekaterina M. Vetchinkina and Alexey V. Babakov

    Citation: BMC Biotechnology 2016 16:43

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