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  1. Several different cDNA labeling methods have been developed for microarray based gene expression analysis. We have examined the accuracy and reproducibility of such five commercially available methods in detec...

    Authors: Azadeh Badiee, Hans Geir Eiken, Vidar M Steen and Roger Løvlie

    Citation: BMC Biotechnology 2003 3:23

    Content type: Methodology article

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  2. The accuracy and precision of estimates of DNA concentration are critical factors for efficient use of DNA samples in high-throughput genotype and sequence analyses. We evaluated the performance of spectrophot...

    Authors: Kashif A Haque, Ruth M Pfeiffer, Michael B Beerman, Jeff P Struewing, Stephen J Chanock and Andrew W Bergen

    Citation: BMC Biotechnology 2003 3:20

    Content type: Methodology article

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  3. C. elegans has been established as a powerful genetic system. Use of a chemically defined medium (C. elegans Maintenance Medium (CeMM)) now allows standardization and systematic manipulation of the nutrients that...

    Authors: Nathaniel J Szewczyk, Elena Kozak and Catharine A Conley

    Citation: BMC Biotechnology 2003 3:19

    Content type: Methodology article

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  4. Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR produc...

    Authors: Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs and Alex F Markham

    Citation: BMC Biotechnology 2003 3:18

    Content type: Methodology article

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  5. Expression of human Interleukin-5 receptor alpha (hIL-5Rα) is controlled by alternative splicing, which generates two different transcripts encoding a membrane-anchored and a soluble form of the receptor, resp...

    Authors: Claudina Pérez, Jo Vandesompele, Ina Vandenbroucke, Gabriele Holtappels, Frank Speleman, Philippe Gevaert, Paul Van cauwenberge and Claus Bachert

    Citation: BMC Biotechnology 2003 3:17

    Content type: Methodology article

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  6. Gene transfer into the amniotic fluid using recombinant adenovirus vectors was shown previously to result in high efficiency transfer of transgenes into the lungs and intestines. Adenovirus mediated in utero gene...

    Authors: Deiadra J Garrett, Janet E Larson, Daisy Dunn, Luis Marrero and J Craig Cohen

    Citation: BMC Biotechnology 2003 3:16

    Content type: Methodology article

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  7. Interleukin-1 (IL-1) is a cytokine involved in the initiation and amplification of the defence response in infectious and inflammatory diseases. IL-1 receptor antagonist (IL-1ra) is an inactive member of the I...

    Authors: Susanna Ricci, Giovanni Macchia, Paolo Ruggiero, Tiziana Maggi, Paola Bossù, Li Xu, Donata Medaglini, Aldo Tagliabue, Lennart Hammarström, Gianni Pozzi and Diana Boraschi

    Citation: BMC Biotechnology 2003 3:15

    Content type: Research article

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  8. Non-viral vectors for gene transfer are less immunogenic than viral vectors but also less efficient. Significant effort has focused on enhancing non-viral gene transfer efficiency by increasing nuclear import ...

    Authors: Marie Carrière, Virginie Escriou, Aline Savarin and Daniel Scherman

    Citation: BMC Biotechnology 2003 3:14

    Content type: Research article

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  9. cDNA microarrays have the potential to identify the genes involved in invasion and metastasis. However, when used with whole tumor tissue, the results average the expression patterns of different cell types. W...

    Authors: Weigang Wang, Jeffrey B Wyckoff, Yarong Wang, Erwin P Bottinger, Jeffrey E Segall and John S Condeelis

    Citation: BMC Biotechnology 2003 3:13

    Content type: Methodology article

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  10. Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these pro...

    Authors: Christoph Scheich, Volker Sievert and Konrad Büssow

    Citation: BMC Biotechnology 2003 3:12

    Content type: Methodology article

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  11. The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as po...

    Authors: James Butz, Eric Wickstrom and Jeremy Edwards

    Citation: BMC Biotechnology 2003 3:11

    Content type: Methodology article

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  12. The interest in microfluidics and surface patterning is increasing as the use of these technologies in diverse biomedical applications is substantiated. Controlled molecular and cellular surface patterning is ...

    Authors: Thomas Brevig, Ulrich Krühne, Rachel A Kahn, Thomas Ahl, Michael Beyer and Lars H Pedersen

    Citation: BMC Biotechnology 2003 3:10

    Content type: Methodology article

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  13. Homology-dependent selective degradation of RNA, or post-transcriptional gene silencing (PTGS), is involved in several biological phenomena, including adaptative defense mechanisms against plant viruses. Small...

    Authors: Tiziana Pandolfini, Barbara Molesini, Linda Avesani, Angelo Spena and Annalisa Polverari

    Citation: BMC Biotechnology 2003 3:7

    Content type: Research article

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  14. DNA microarrays are now routinely used to monitor the transcript levels of thousands of genes simultaneously. However, the array fabrication method, hybridization conditions, and oligodeoxyribonucleotide probe...

    Authors: David R Dorris, Allen Nguyen, Linn Gieser, Randall Lockner, Anna Lublinsky, Marcus Patterson, Edward Touma, Timothy J Sendera, Robert Elghanian and Abhijit Mazumder

    Citation: BMC Biotechnology 2003 3:6

    Content type: Research article

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  15. Double-stranded RNA (dsRNA) is a potent initiator of gene silencing in a diverse group of organisms that includes plants, Caenorhabditis elegans, Drosophila and mammals. We have previously shown and patented that...

    Authors: Francisco Tenllado, Belén Martínez-García, Marisol Vargas and José Ramón Díaz-Ruíz

    Citation: BMC Biotechnology 2003 3:3

    Content type: Research article

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  16. Bacterial artificial chromosomes (BACs) have been used extensively for sequencing the human and mouse genomes and are thus readily available for most genes. The large size of BACs means that they can generally...

    Authors: Christine Magin-Lachmann, George Kotzamanis, Leonardo D'Aiuto, Ernst Wagner and Clare Huxley

    Citation: BMC Biotechnology 2003 3:2

    Content type: Research article

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  17. The ability to manipulate the genetic networks underlying the physiological and behavioural repertoires of the adult honeybee worker (Apis mellifera) is likely to deepen our understanding of issues such as learni...

    Authors: Gro V Amdam, Zilá LP Simões, Karina R Guidugli, Kari Norberg and Stig W Omholt

    Citation: BMC Biotechnology 2003 3:1

    Content type: Methodology article

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  18. Allosteric ribozymes (aptazymes) that have extraordinary activation parameters have been generated in vitro by design and selection. For example, hammerhead and ligase ribozymes that are activated by small organi...

    Authors: Kristin M Thompson, Heather A Syrett, Scott M Knudsen and Andrew D Ellington

    Citation: BMC Biotechnology 2002 2:21

    Content type: Research article

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  19. When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this...

    Authors: Giovanna Mason, Paolo Provero, Anna Maria Vaira and Gian Paolo Accotto

    Citation: BMC Biotechnology 2002 2:20

    Content type: Methodology article

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  20. Ostrich (Struthio camelus) breeds have been gaining increasing significance around the world. The large-scale sex determination of chicks is an important task in the development of these breeds. To date, two PCR-...

    Authors: Wilson Malagó Jr, Heitor M Franco, Euclides Matheucci Jr, Adriana Medaglia and Flavio Henrique-Silva

    Citation: BMC Biotechnology 2002 2:19

    Content type: Methodology article

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  21. Efficient transformation and regeneration methods are a priority for successful application of genetic engineering to vegetative propagated plants such as grape. The current methods for the production of trans...

    Authors: Bruno Mezzetti, Tiziana Pandolfini, Oriano Navacchi and Lucia Landi

    Citation: BMC Biotechnology 2002 2:18

    Content type: Research article

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  22. The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. It has also been suggested for screening pharmacological activities in plant extracts. However, we think tha...

    Authors: José Luis Carballo, Zaira L Hernández-Inda, Pilar Pérez and María D García-Grávalos

    Citation: BMC Biotechnology 2002 2:17

    Content type: Methodology article

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  23. Hep27 monoclonal (Hep27 Mab) is an antibody against hepatocellular carcinoma. Hep27 Mab itself can inhibit the growth of a hepatocellular carcinoma cell line (HCC-S102). We attempted to produce a single-chain ...

    Authors: Duanpen Sandee, Sumalee Tungpradabkul, Manae Tsukio, Tadayuki Imanaka and Masahiro Takagi

    Citation: BMC Biotechnology 2002 2:16

    Content type: Research article

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  24. The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high produc...

    Authors: Eric Devroe and Pamela A Silver

    Citation: BMC Biotechnology 2002 2:15

    Content type: Methodology article

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  25. The power of DNA microarrays derives from their ability to monitor the expression levels of many genes in parallel. One of the limitations of such powerful analytical tools is the inability to detect certain t...

    Authors: Allen Nguyen, Connie Zhao, David Dorris and Abhijit Mazumder

    Citation: BMC Biotechnology 2002 2:14

    Content type: Research article

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  26. In a variant of the standard PCR reaction termed bridging, or jumping, PCR the primer-bound sequences are originally on separate template molecules. Bridging can occur if, and only if, the templates contain a ...

    Authors: Shumo Liu, David S Thaler and Albert Libchaber

    Citation: BMC Biotechnology 2002 2:13

    Content type: Research article

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  27. Cationic lipid DNA complexes based on DOTAP (1,2-dioleoyl-3-(trimethyammonium) propane) and mixtures of DOTAP and cholesterol (DC) have been previously optimized for transfection efficiency in the absence of s...

    Authors: Godwin Nchinda, Klaus Überla and Olaf Zschörnig

    Citation: BMC Biotechnology 2002 2:12

    Content type: Research article

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  28. Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria ...

    Authors: Anna-Katerina Hadjantonakis, Suzanne Macmaster and Andras Nagy

    Citation: BMC Biotechnology 2002 2:11

    Content type: Methodology article

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  29. This study tested a low-volume (20–30 μl/20–30 μg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation o...

    Authors: Shuxun Ren, Minglin Li, Joanne M Smith, Louis J DeTolla and Priscilla A Furth

    Citation: BMC Biotechnology 2002 2:10

    Content type: Methodology article

    Published on:

  30. We investigated the encapsulation mechanism of enzymes into liposomes. The existing protocols to achieve high encapsulation efficiencies are basically optimized for chemically stable molecules. Enzymes, howeve...

    Authors: Jacques-Philippe Colletier, Barnabé Chaize, Mathias Winterhalter and Didier Fournier

    Citation: BMC Biotechnology 2002 2:9

    Content type: Methodology article

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  31. Determination of clot lysis times on whole blood, diluted whole blood, plasma or plasma fraction has been used for many years to assess the overall activity of the fibrinolytic system. We designed a completely...

    Authors: K Zouaoui Boudjeltia, Ph Cauchie, Cl Remacle, M Guillaume, D Brohée, JL Hubert and M Vanhaeverbeek

    Citation: BMC Biotechnology 2002 2:8

    Content type: Methodology article

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  32. Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limi...

    Authors: Keejong Chang, Jin Qian, MeiSheng Jiang, Yi-Hsin Liu, Ming-Che Wu, Chi-Dar Chen, Chao-Kuen Lai, Hsin-Lung Lo, Chin-Ton Hsiao, Lucy Brown, James Bolen Jr, Hsiao-I Huang, Pei-Yu Ho, Ping Yao Shih, Chen-Wen Yao, Wey-Jinq Lin…

    Citation: BMC Biotechnology 2002 2:5

    Content type: Research article

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  33. Parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the DefH9-iaaM gene. The iaaM gene codes for tryptophan...

    Authors: Nazzareno Acciarri, Federico Restaino, Gabriele Vitelli, Domenico Perrone, Michela Zottini, Tiziana Pandolfini, Angelo Spena and Giuseppe Leonardo Rotino

    Citation: BMC Biotechnology 2002 2:4

    Content type: Research article

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  34. The suitability of the strain Rhodococcus erythropolis ATCC 25544 grown in a two-liter fermentor as a source of cholesterol oxidase has been investigated. The strain produces both cell-linked and extracellular ch...

    Authors: Mar M Sojo, Roque R Bru and Francisco F García-Carmona

    Citation: BMC Biotechnology 2002 2:3

    Content type: Research article

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  35. Agrobacterium vitis is a causal agent of crown-gall disease. Trifolitoxin (TFX) is a peptide antibiotic active only against members of a specific group of α-proteobacteria that includes Agrobacterium and its clos...

    Authors: Thomas C Herlache and Eric W Triplett

    Citation: BMC Biotechnology 2002 2:2

    Content type: Research article

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  36. Genetic engineering of parthenocarpy confers to horticultural plants the ability to produce fruits under environmental conditions that curtail fruit productivity and quality. The DefH9-iaaM transgene, whose predi...

    Authors: Tiziana Pandolfini, Giuseppe L Rotino, Serena Camerini, Roberto Defez and Angelo Spena

    Citation: BMC Biotechnology 2002 2:1

    Content type: Methodology article

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  37. The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its promoter. A combined in vitro / in vivo sele...

    Authors: Jijumon Chelliserrykattil, George Cai and Andrew D Ellington

    Citation: BMC Biotechnology 2001 1:13

    Content type: Research article

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  38. Transgenic mice have been used extensively to analyze gene function. Unfortunately, traditional transgenic procedures have only limited use in analyzing alleles that cause lethality because lines of founder mi...

    Authors: Ravi P Misra, Sarah K Bronson, Qi Xiao, Wendy Garrison, Jixuan Li, Roong Zhao and Stephen A Duncan

    Citation: BMC Biotechnology 2001 1:12

    Content type: Methodology article

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  39. Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and ...

    Authors: Larry R Karns PhD, Anne Kisielewski BSc, Kathryn M Gulding BSc and Dan Theodorescu MD PhD

    Citation: BMC Biotechnology 2001 1:11

    Content type: Research article

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  40. To maintain populations of microbial cells under controlled conditions of growth and environment for an indefinite duration is a prerequisite for experimentally evolving natural isolates of wild-type species o...

    Authors: Valérie A de Crécy-Lagard, Jacques Bellalou, Rupert Mutzel and Philippe Marlière

    Citation: BMC Biotechnology 2001 1:10

    Content type: Methodology article

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