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  1. The most efficient method for enhancing solubility of recombinant proteins appears to use the fusion expression partners. Although commercial fusion partners including maltose binding protein and glutathione-S-tr...

    Authors: Jin-Seung Park, Kyung-Yeon Han, Jong-Ho Lee, Jong-Am Song, Keum-Young Ahn, Hyuk-Seong Seo, Sang-Jun Sim, Seung-Wook Kim and Jeewon Lee
    Citation: BMC Biotechnology 2008 8:15
  2. Rhipicephalus (Boophilus) spp. ticks economically impact on cattle production in Africa and other tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally fr...

    Authors: Mario Canales, José M Pérez de la Lastra, Victoria Naranjo, Ard M Nijhof, Michelle Hope, Frans Jongejan and José de la Fuente
    Citation: BMC Biotechnology 2008 8:14
  3. Human cell lines are the most innovative choice of host cell for production of biopharmaceuticals since they allow for authentic posttranslational modification of therapeutic proteins. We present a new method ...

    Authors: Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Waschütza and Christoph Volpers
    Citation: BMC Biotechnology 2008 8:13
  4. Combination of CHD (chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-inten...

    Authors: Hsueh-Wei Chang, Chun-An Cheng, De-Leung Gu, Chia-Che Chang, San-Hua Su, Cheng-Hao Wen, Yii-Cheng Chou, Ta-Ching Chou, Cheng-Te Yao, Chi-Li Tsai and Chien-Chung Cheng
    Citation: BMC Biotechnology 2008 8:12
  5. Elevations of serum prolactin (PRL) are associated with an increased risk for breast cancer. PRL signaling through its prolactin receptor (PRLr) involves the Jak2/Stat5 pathway. Luciferase-based reporter assay...

    Authors: Feng Fang, Giovanni Antico, Jiamao Zheng and Charles V Clevenger
    Citation: BMC Biotechnology 2008 8:11
  6. Archival formalin-fixed paraffin-embedded (FFPE) tissues represent an abundant source of clinical specimens; however their use is limited in applications involving analysis of gene expression due to RNA degrad...

    Authors: Jinghuan Li, Paul Smyth, Susanne Cahill, Karen Denning, Richard Flavin, Sinead Aherne, Marco Pirotta, Simone M Guenther, John J O'Leary and Orla Sheils
    Citation: BMC Biotechnology 2008 8:10
  7. Previous studies indicated that recombinant gas vesicles (r-GV) from a mutant strain of Halobacterium sp. NRC-1 could express a cassette containing test sequences of SIVmac gag derived DNA, and function as an ant...

    Authors: Marinko Sremac and Elizabeth S Stuart
    Citation: BMC Biotechnology 2008 8:9
  8. Enzymes have found extensive and growing application in the field of chemical organic synthesis and resolution of chiral intermediates. In order to stabilise the enzymes and to facilitate their recovery and re...

    Authors: Dean Brady, Justin Jordaan, Clinton Simpson, Avashnee Chetty, Cherise Arumugam and Francis S Moolman
    Citation: BMC Biotechnology 2008 8:8
  9. Femtosecond (fs) laser pulses have recently received wide interest as an alternative tool for manipulating living biological systems. In various model organisms the excision of cellular components and the intr...

    Authors: Vikram Kohli and Abdulhakem Y Elezzabi
    Citation: BMC Biotechnology 2008 8:7
  10. High purity of tumour samples is a necessity for accurate genetic and expression analysis and is usually achieved by positive selection in chronic lymphocytic leukaemia (CLL).

    Authors: Salim Essakali, Dennis Carney, David Westerman, Peter Gambell, John F Seymour and Alexander Dobrovic
    Citation: BMC Biotechnology 2008 8:6
  11. Expression of intracellular antibodies (intrabodies) has become a broadly applicable technology for generation of phenotypic knockouts in vivo. The method uses surface depletion of cellular membrane proteins to e...

    Authors: Khajornsak Tragoolpua, Nutjeera Intasai, Watchara Kasinrerk, Sabine Mai, Yuan Yuan and Chatchai Tayapiwatana
    Citation: BMC Biotechnology 2008 8:5
  12. For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneou...

    Authors: Zhengbing Jiang, Bei Gao, Ren Ren, Xingyi Tao, Yushu Ma and Dongzhi Wei
    Citation: BMC Biotechnology 2008 8:4
  13. The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such,...

    Authors: Natalie Gavrilov-Yusim, Ekaterina Hahiashvili, Marina Tashker, Victoria Yavelsky, Ohad Karnieli and Leslie Lobel
    Citation: BMC Biotechnology 2008 8:3
  14. A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules.

    Authors: Angelika Wegerer, Tianqi Sun and Josef Altenbuchner
    Citation: BMC Biotechnology 2008 8:2
  15. Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis.

    Authors: Ricardo Pinto, António L Amaral, Eugénio C Ferreira, Manuel Mota, Manuel Vilanova, Katia Ruel and Miguel Gama
    Citation: BMC Biotechnology 2008 8:1
  16. HERG potassium channel blockade is the major cause for drug-induced long QT syndrome, which sometimes cause cardiac disrhythmias and sudden death. There is a strong interest in the pharmaceutical industry to d...

    Authors: Mao Xiang Chen, Shaun L Sandow, Virginie Doceul, Yu Hua Chen, Heather Harper, Bruce Hamilton, Helen J Meadows, Derek J Trezise and Jeff J Clare
    Citation: BMC Biotechnology 2007 7:93
  17. GFP-fusion proteins and immunostaining are methods broadly applied to investigate the three-dimensional organization of cells and cell nuclei, the latter often studied in addition by fluorescence in situ hybri...

    Authors: Il-Han Kim, Jens Nagel, Simone Otten, Boris Knerr, Roland Eils, Karl Rohr and Steffen Dietzel
    Citation: BMC Biotechnology 2007 7:92
  18. Due to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed...

    Authors: Torstein Tengs, Anja B Kristoffersen, Knut G Berdal, Tage Thorstensen, Melinka A Butenko, HÃ¥vard Nesvold and Arne Holst-Jensen
    Citation: BMC Biotechnology 2007 7:91
  19. Bone marrow-derived stromal cells (MSC) are attractive targets for ex vivo cell and gene therapy. In this context, we investigated the feasibility of a plasmid-based strategy for genetic modification of human (h)...

    Authors: Mark W Ronsyn, Jasmijn Daans, Gie Spaepen, Shyama Chatterjee, Katrien Vermeulen, Patrick D'Haese, Viggo FI Van Tendeloo, Eric Van Marck, Dirk Ysebaert, Zwi N Berneman, Philippe G Jorens and Peter Ponsaerts
    Citation: BMC Biotechnology 2007 7:90
  20. The acute sensitivity of some hybridoma cell lines to culture-related stresses severely limits their productivity. Recent developments in the characterization of the stress signals modulating the cellular phen...

    Authors: Matthew Mallory, Kevin Chartrand and Eric R Gauthier
    Citation: BMC Biotechnology 2007 7:89
  21. Conventional cell culture studies have been performed on 2D surfaces, resulting in flat, extended cell growth. More relevant studies are desired to better mimic 3D in vivo tissue growth. Such realistic environmen...

    Authors: Thomas Liebmann, Susanna Rydholm, Victor Akpe and Hjalmar Brismar
    Citation: BMC Biotechnology 2007 7:88
  22. The formation of two distinctive cell lineages in preimplantation mouse embryos is characterized by differential gene expression. The cells of the inner cell mass are pluripotent and express high levels of Oct4 m...

    Authors: Cristina Hartshorn, Judith J Eckert, Odelya Hartung and Lawrence J Wangh
    Citation: BMC Biotechnology 2007 7:87
  23. Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. Often, it is used in immobilised form. With conventional immobilisation t...

    Authors: Barry J Ryan and Ciarán Ó'Fágáin
    Citation: BMC Biotechnology 2007 7:86
  24. Retroviral vectors are valuable tools for gene transfer. Particularly convenient are IRES-containing retroviral vectors expressing both the protein of interest and a marker protein from a single bicistronic mR...

    Authors: Olivier Albagli-Curiel, Yann Lécluse, Philippe Pognonec, Kim E Boulukos and Patrick Martin
    Citation: BMC Biotechnology 2007 7:85
  25. Antibody-dependent cellular cytotoxicity (ADCC) is greatly enhanced by the absence of the core fucose of oligosaccharides attached to the Fc, and is closely related to the clinical efficacy of anticancer activ...

    Authors: Harue Imai-Nishiya, Katsuhiro Mori, Miho Inoue, Masako Wakitani, Shigeru Iida, Kenya Shitara and Mitsuo Satoh
    Citation: BMC Biotechnology 2007 7:84
  26. Virus-mediated delivery of therapeutic transgenes to the inflamed colon holds a great potential to serve as an effective therapeutic strategy for inflammatory bowel disease, since local, long-term expression o...

    Authors: Samuel J Farlow, Alan Jerusalmi and Takeshi Sano
    Citation: BMC Biotechnology 2007 7:83
  27. DNA microarrays are among the most widely used technical platforms for DNA and RNA studies, and issues related to microarrays sensitivity and specificity are therefore of general importance in life sciences. C...

    Authors: Nicoletta Mascellani, Xiuping Liu, Simona Rossi, Jlenia Marchesini, Davide Valentini, Diego Arcelli, Cristian Taccioli, Mauro Helmer Citterich, Chang-Gong Liu, Rita Evangelisti, Giandomenico Russo, Jorge M Santos, Carlo M Croce and Stefano Volinia
    Citation: BMC Biotechnology 2007 7:82
  28. Intrabodies are defined as antibody molecules which are ectopically expressed inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in living cells. However, most antibody ...

    Authors: Pascal Philibert, Audrey Stoessel, Wei Wang, Annie-Paule Sibler, Nicole Bec, Christian Larroque, Jeffery G Saven, Jérôme Courtête, Etienne Weiss and Pierre Martineau
    Citation: BMC Biotechnology 2007 7:81
  29. RSF1010 is a well-studied broad-host-range plasmid able to be mobilized to different bacteria and plants. RSF1010-derived plasmid vectors are widely used in both basic research and industrial applications. In ...

    Authors: Joanna I Katashkina, Tatiana M Kuvaeva, Irina G Andreeva, Alexandra Yu Skorokhodova, Irina V Biryukova, Irina L Tokmakova, Lubov I Golubeva and Sergey V Mashko
    Citation: BMC Biotechnology 2007 7:80
  30. RNA polymerase III (pol III) type 3 promoters such as U6 or 7SK are commonly used to express short-hairpin RNA (shRNA) effectors for RNA interference (RNAi). To extend the use of RNAi for studies of developmen...

    Authors: Stephanie C Bannister, Terry G Wise, David M Cahill and Timothy J Doran
    Citation: BMC Biotechnology 2007 7:79
  31. Shark heavy chain antibody, also called new antigen receptor (NAR), consists of one single Variable domain (VH), containing only two complementarity-determining regions (CDRs). The antigen binding affinity and sp...

    Authors: Jinny L Liu, George P Anderson and Ellen R Goldman
    Citation: BMC Biotechnology 2007 7:78
  32. The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, b...

    Authors: Javier F Chaparro-Riggers, Bernard LW Loo, Karen M Polizzi, Phillip R Gibbs, Xiao-Song Tang, Mark J Nelson and Andreas S Bommarius
    Citation: BMC Biotechnology 2007 7:77
  33. Adjustable gene expression is crucial in a number of applications such as de- or transdifferentiation of cell phenotypes, tissue engineering, various production processes as well as gene-therapy initiatives. V...

    Authors: David A Fluri, Marie Daoud-El Baba and Martin Fussenegger
    Citation: BMC Biotechnology 2007 7:75
  34. Electrotransfer of plasmid DNA into skeletal muscle is a promising strategy for the delivery of therapeutic molecules targeting various muscular diseases, cancer and lower-limb ischemia. Internal Ribosome Entr...

    Authors: Camille Allera-Moreau, Aurélie Delluc-Clavières, Caroline Castano, Loïc Van den Berghe, Muriel Golzio, Marc Moreau, Justin Teissié, Jean-François Arnal and Anne-Catherine Prats
    Citation: BMC Biotechnology 2007 7:74
  35. The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One way to increase AP-3 production would be to consti...

    Authors: Shan Goh, Andrea Camattari, Daniel Ng, Ruth Song, Kevin Madden, Janet Westpheling and Victor VT Wong
    Citation: BMC Biotechnology 2007 7:72
  36. Genomics tools, particularly DNA microarrays, have found application in a number of areas including gene discovery and disease characterization. Despite the vast utility of these tools, little work has been do...

    Authors: Pratik Jaluria, Michael Betenbaugh, Konstantinos Konstantopoulos and Joseph Shiloach
    Citation: BMC Biotechnology 2007 7:71
  37. There is much evidence that tumor cells elicit a humoral immune response in patients. In most cases, the presence of antibodies in peripheral blood is detected only in small proportion of patients with tumors ...

    Authors: Emiliano Pavoni, Giorgia Monteriù, Daniela Santapaola, Fiorella Petronzelli, Anna Maria Anastasi, Angela Pelliccia, Valeria D'Alessio, Rita De Santis and Olga Minenkova
    Citation: BMC Biotechnology 2007 7:70
  38. In situ detection is traditionally performed with long labeled probes often followed by a signal amplification step to enhance the labeling. Whilst short probes have several advantages over long probes (e.g. h...

    Authors: Magnus Stougaard, Jakob S Lohmann, Magdalena Zajac, Stephen Hamilton-Dutoit and Jørn Koch
    Citation: BMC Biotechnology 2007 7:69
  39. The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capabi...

    Authors: Gaetano Donofrio, Chiara Sartori, Lara Ravanetti, Sandro Cavirani, Laurent Gillet, Alain Vanderplasschen, Simone Taddei and Cesidio Filippo Flammini
    Citation: BMC Biotechnology 2007 7:68
  40. Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to evaluate in vivo multiplex imaging of mouse embryonic stem (ES) cells labeled...

    Authors: Shuan Lin, Xiaoyan Xie, Manishkumar R Patel, Yao-Hung Yang, Zongjin Li, Feng Cao, Oliver Gheysens, Yan Zhang, Sanjiv S Gambhir, Jiang Hong Rao and Joseph C Wu
    Citation: BMC Biotechnology 2007 7:67
  41. High content screening (HCS)-based image analysis is becoming an important and widely used research tool. Capitalizing this technology, ample cellular information can be extracted from the high content cellula...

    Authors: Fuhai Li, Xiaobo Zhou, Jinmin Zhu, Jinwen Ma, Xudong Huang and Stephen TC Wong
    Citation: BMC Biotechnology 2007 7:66
  42. Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic pha...

    Authors: Lauren RH Krumpe, Kathryn M Schumacher, James B McMahon, Lee Makowski and Toshiyuki Mori
    Citation: BMC Biotechnology 2007 7:65
  43. The growing field of proteomics and systems biology is resulting in an ever increasing demand for purified recombinant proteins for structural and functional studies. Here, we show a systematic approach to suc...

    Authors: Claudia Langlais, Birgit Guilleaume, Nadja Wermke, Tina Scheuermann, Lars Ebert, Joshua LaBaer and Bernhard Korn
    Citation: BMC Biotechnology 2007 7:64
  44. RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner.

    Authors: Stéphanie Jaubert-Possamai, Gaël Le Trionnaire, Joël Bonhomme, Georges K Christophides, Claude Rispe and Denis Tagu
    Citation: BMC Biotechnology 2007 7:63
  45. Plant viruses can be employed as versatile vectors for the production of vaccines by expressing immunogenic epitopes on the surface of chimeric viral particles. Although several viruses, including tobacco mosa...

    Authors: Chung-Da Yang, Jia-Teh Liao, Chen-Yen Lai, Ming-Hwa Jong, Chi-Ming Liang, Yeou-Liang Lin, Na-Sheng Lin, Yau-Heiu Hsu and Shu-Mei Liang
    Citation: BMC Biotechnology 2007 7:62
  46. Conditional expression vectors have become a valuable research tool to avoid artefacts that may result from traditional gene expression studies. However, most systems require multiple plasmids that must be ind...

    Authors: Daniel C Gray, Klaus P Hoeflich, Li Peng, Zhenyu Gu, Alvin Gogineni, Lesley J Murray, Mike Eby, Noelyn Kljavin, Somasekar Seshagiri, Mary J Cole and David P Davis
    Citation: BMC Biotechnology 2007 7:61
  47. Microfluidics is an enabling technology with a number of advantages over traditional tissue culture methods when precise control of cellular microenvironment is required. However, there are a number of practic...

    Authors: Bong Geun Chung, Jeong Won Park, Jia Sheng Hu, Carlos Huang, Edwin S Monuki and Noo Li Jeon
    Citation: BMC Biotechnology 2007 7:60

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