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Page 29 of 30

  1. Uromodulin is the most abundant protein found in the urine of mammals. In an effort to utilize the uromodulin promoter in order to target recombinant proteins in the urine of transgenic animals we have cloned ...

    Authors: Yue-Jin Huang, Nathalie Chretien, Annie S Bilodeau, Jiang Feng Zhou, Anthoula Lazaris and Costas N Karatzas

    Citation: BMC Biotechnology 2005 5:9

    Content type: Research article

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  2. We have developed an oligonucleotide microarray (genosensor) utilizing a double tandem hybridization technique to search for 9 point mutations located in the most frequently altered codons of the TP53 gene. Isola...

    Authors: Angélica Rangel-López, Rogelio Maldonado-Rodríguez, Mauricio Salcedo-Vargas, Juana Mercedes Espinosa-Lara, Alfonso Méndez-Tenorio and Kenneth L Beattie

    Citation: BMC Biotechnology 2005 5:8

    Content type: Methodology article

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  3. The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic s...

    Authors: Sreelatha Meleth, Jessy Deshane and Helen Kim

    Citation: BMC Biotechnology 2005 5:7

    Content type: Research article

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  5. Transcriptional profiling using microarrays has developed into a key molecular tool for the elucidation of gene function and gene regulation. Microarray platforms based on either oligonucleotides or purified a...

    Authors: Valtteri Wirta, Anders Holmberg, Morten Lukacs, Peter Nilsson, Pierre Hilson, Mathias Uhlén, Rishikesh P Bhalerao and Joakim Lundeberg

    Citation: BMC Biotechnology 2005 5:5

    Content type: Methodology article

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  6. Common oral diseases and dental caries can be prevented effectively by passive immunization. In humans, passive immunotherapy may require the use of humanized or human antibodies to prevent adverse immune resp...

    Authors: Michael B Kupper, Michael Huhn, Holger Spiegel, Julian KC Ma, Stefan Barth, Rainer Fischer and Ricarda Finnern

    Citation: BMC Biotechnology 2005 5:4

    Content type: Research article

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  7. In the past ten years there has been a growing interest in engineering Gram-positive bacteria for biotechnological applications, including vaccine delivery and production of recombinant proteins. Usually, bact...

    Authors: Roberta Provvedi, Tiziana Maggi, Marco R Oggioni, Riccardo Manganelli and Gianni Pozzi

    Citation: BMC Biotechnology 2005 5:3

    Content type: Methodology article

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  8. Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is part...

    Authors: Cristina Hartshorn, Aleksandra Anshelevich and Lawrence J Wangh

    Citation: BMC Biotechnology 2005 5:2

    Content type: Methodology article

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  9. Recombinant adenoviral vectors are highly efficient for in vitro and in vivo gene delivery. They can easily be produced in large numbers, transduce a wide variety of cell types and generate high levels of tran...

    Authors: Dalibor Antolovic, Moritz Koch, Inga Bohlmann, Peter Kienle, Markus Büchler and Jürgen Weitz

    Citation: BMC Biotechnology 2005 5:1

    Content type: Methodology article

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  11. In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied i...

    Authors: Michael R Dyson, S Paul Shadbolt, Karen J Vincent, Rajika L Perera and John McCafferty

    Citation: BMC Biotechnology 2004 4:32

    Content type: Research article

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  12. Modification of leaf traits in sugar beet requires a strong leaf specific promoter. With such a promoter, expression in taproots can be avoided which may otherwise take away available energy resources for suga...

    Authors: Dietmar J Stahl, Dorothee U Kloos and Reinhard Hehl

    Citation: BMC Biotechnology 2004 4:31

    Content type: Research article

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  13. Laser microdissection allows precise isolation of specific cell types and compartments from complex tissues. To analyse proteins from small cell numbers, we combine laser-microdissection and manipulation (LMM)...

    Authors: Grazyna Kwapiszewska, Markus Meyer, Ralf Bogumil, Rainer M Bohle, Werner Seeger, Norbert Weissmann and Ludger Fink

    Citation: BMC Biotechnology 2004 4:30

    Content type: Methodology article

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  14. The blasticidin S resistance gene (bsr) is a selectable marker used for gene transfer experiments. The bsr gene encodes for blasticidin S (BS) deaminase, which has a specific activity upon BS. Therefore, its expr...

    Authors: Fernanda Mara Bento, Daniela Takeshita, Chester Bittencourt Sacramento, Tamara Rocha Machado, Monica Beatriz Mathor, Adriana Karaoglanovic Carmona and Sang Won Han

    Citation: BMC Biotechnology 2004 4:29

    Content type: Research article

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  15. Fast, cheap and reliable methods are needed to identify large populations, which may be at risk in relation to environmental exposure. Polymorphisms in NAT1 (N-acetyl transferase) may be suitable markers to id...

    Authors: Pavel Soucek, Camilla Furu Skjelbred, Marit Svendsen, Tom Kristensen, Elin H Kure and Vessela N Kristensen

    Citation: BMC Biotechnology 2004 4:28

    Content type: Methodology article

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  16. Mucosal delivery of therapeutic protein drugs or vaccines is actively investigated, in order to improve bioavailability and avoid side effects associated with systemic administration. Orally administered bacte...

    Authors: Stefano Porzio, Paola Bossù, Paolo Ruggiero, Diana Boraschi and Aldo Tagliabue

    Citation: BMC Biotechnology 2004 4:27

    Content type: Research article

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  19. Each of the human genes or transcriptional units is likely to contain single nucleotide polymorphisms that may give rise to sequence variation between individuals and tissues on the level of RNA. Based on rece...

    Authors: Ulrika Liljedahl, Mona Fredriksson, Andreas Dahlgren and Ann-Christine Syvänen

    Citation: BMC Biotechnology 2004 4:24

    Content type: Methodology article

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  20. Several cell lines and primary cultures benefit from the use of positively charged extracellular matrix proteins or polymers that enhance their ability to attach to culture plates. Polyethyleneimine is a posit...

    Authors: Ajith R Vancha, Suman Govindaraju, Kishore VL Parsa, Madhuri Jasti, Maribel González-García and Rafael P Ballestero

    Citation: BMC Biotechnology 2004 4:23

    Content type: Methodology article

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  21. Bioleaching is a process that has been used in the past in mineral pretreatment of refractory sulfides, mainly in the gold, copper and uranium benefit. This technology has been proved to be cheaper, more effic...

    Authors: Mario Makita, Margarita Esperón, Benito Pereyra, Alejandro López and Erasmo Orrantia

    Citation: BMC Biotechnology 2004 4:22

    Content type: Research article

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  22. Cell migration is a complex phenomenon that requires the coordination of numerous cellular processes. Investigation of cell migration and its underlying biology is of interest to basic scientists and those in ...

    Authors: Justin C Yarrow, Zachary E Perlman, Nicholas J Westwood and Timothy J Mitchison

    Citation: BMC Biotechnology 2004 4:21

    Content type: Methodology article

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  25. RNA interference (RNAi) in animals and post-transcriptional gene silencing (PTGS) in plants are related phenomena whose functions include the developmental regulation of gene expression and protection from tra...

    Authors: Brian Reavy, Sheila Dawson, Tomas Canto and Stuart A MacFarlane

    Citation: BMC Biotechnology 2004 4:18

    Content type: Research article

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  26. There are now several ways to generate fluorescent fusion proteins by randomly inserting DNA encoding the Green Fluorescent Protein (GFP) into another protein's coding sequence. These approaches can be used to...

    Authors: Douglas L Sheridan and Thomas E Hughes

    Citation: BMC Biotechnology 2004 4:17

    Content type: Methodology article

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  27. Polycistronic retroviral vectors that contain several therapeutic genes linked via internal ribosome entry sites (IRES), provide new and effective tools for the co-expression of exogenous cDNAs in clinical gen...

    Authors: Victorine Douin, Stephanie Bornes, Laurent Creancier, Philippe Rochaix, Gilles Favre, Anne-Catherine Prats and Bettina Couderc

    Citation: BMC Biotechnology 2004 4:16

    Content type: Research article

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  28. Sustainable DNA resources and reliable high-throughput genotyping methods are required for large-scale, long-term genetic association studies. In the genetic dissection of common disease it is now recognised t...

    Authors: Rebecca Pask, Helen E Rance, Bryan J Barratt, Sarah Nutland, Deborah J Smyth, Meera Sebastian, Rebecca CJ Twells, Anne Smith, Alex C Lam, Luc J Smink, Neil M Walker and John A Todd

    Citation: BMC Biotechnology 2004 4:15

    Content type: Methodology article

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  30. In addition to studies of plant gene function and developmental analyses, plant biotechnological use is largely dependent upon transgenic technologies. The moss Physcomitrella patens has become an exciting model ...

    Authors: Verena Horstmann, Claudia M Huether, Wolfgang Jost, Ralf Reski and Eva L Decker

    Citation: BMC Biotechnology 2004 4:13

    Content type: Research article

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  31. RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited ...

    Authors: Fekadu Yadetie, Arne K Sandvik, Hallgeir Bergum, Kristin Norsett and Astrid Laegreid

    Citation: BMC Biotechnology 2004 4:12

    Content type: Methodology article

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  32. Electroporation (EP) is a widely used non-viral gene transfer method. We have attempted to develop an exact protocol to maximize DNA expression while minimizing tissue damage following EP of skeletal muscle in vi...

    Authors: Jordan Taylor, Charlie F Babbs, Mohammed Borhan Alzghoul, Aaron Olsen, Mickey Latour, Amber L Pond and Kevin Hannon

    Citation: BMC Biotechnology 2004 4:11

    Content type: Methodology article

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  33. Members of the GATA transcription factor family have been used in many transfection studies to investigate their roles in the regulation of gene expression. To correct for variations in transfection efficiency...

    Authors: Clement KM Ho and Jerome F Strauss III

    Citation: BMC Biotechnology 2004 4:10

    Content type: Research article

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  34. Whether for cell culture studies of protein function, construction of mouse models to enable in vivo analysis of disease epidemiology, or ultimately gene therapy of human diseases, a critical enabling step is the...

    Authors: Marina Sudomoina, Ekaterina Latypova, Olga O Favorova, Erica A Golemis and Ilya G Serebriiskii

    Citation: BMC Biotechnology 2004 4:9

    Content type: Methodology article

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  35. Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either g...

    Authors: Susanna Harju, Halyna Fedosyuk and Kenneth R Peterson

    Citation: BMC Biotechnology 2004 4:8

    Content type: Methodology article

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  37. Antibody fragments selected from large combinatorial libraries have numerous applications in diagnosis and therapy. Most existing antibody repertoires are derived from human immunoglobulin genes. Genes from ot...

    Authors: Wouter van Wyngaardt, Teresiah Malatji, Cordelia Mashau, Jeanni Fehrsen, Frances Jordaan, Dubravka Miltiadou and Dion H du Plessis

    Citation: BMC Biotechnology 2004 4:6

    Content type: Research article

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  38. Primary hepatocytes, one of the most widely used cell types for toxicological studies, have a very limited life span and must be freshly derived from mice or even humans. Attempts to use stable cell lines main...

    Authors: Maria Grazia Sacco, Laura Amicone, Enrica Mira Catò, Daniela Filippini, Paolo Vezzoni and Marco Tripodi

    Citation: BMC Biotechnology 2004 4:5

    Content type: Methodology article

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  39. The DefH9-iaaM gene fusion which is expressed specifically in placenta/ovules and promotes auxin-synthesis confers parthenocarpic fruit development to eggplant, tomato and tobacco. Transgenic DefH9-iaaM eggplants...

    Authors: Bruno Mezzetti, Lucia Landi, Tiziana Pandolfini and Angelo Spena

    Citation: BMC Biotechnology 2004 4:4

    Content type: Research article

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  40. DNA microarray assays typically compare two biological samples and present the results of those comparisons gene-by-gene as the logarithm base two of the ratio of the measured expression levels for the two sam...

    Authors: Vasily Sharov, Ka Yin Kwong, Bryan Frank, Emily Chen, Jeremy Hasseman, Renee Gaspard, Yan Yu, Ivana Yang and John Quackenbush

    Citation: BMC Biotechnology 2004 4:3

    Content type: Methodology article

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  41. Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by...

    Authors: Rajiv Tyagi, Richard Lai and Ronald G Duggleby

    Citation: BMC Biotechnology 2004 4:2

    Content type: Methodology article

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  42. BAC clones containing entire mammalian genes including all the transcribed region and long range controlling elements are very useful for functional analysis. Sequenced BACs are available for most of the human...

    Authors: George Kotzamanis and Clare Huxley

    Citation: BMC Biotechnology 2004 4:1

    Content type: Methodology article

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  43. Several different cDNA labeling methods have been developed for microarray based gene expression analysis. We have examined the accuracy and reproducibility of such five commercially available methods in detec...

    Authors: Azadeh Badiee, Hans Geir Eiken, Vidar M Steen and Roger Løvlie

    Citation: BMC Biotechnology 2003 3:23

    Content type: Methodology article

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  46. The accuracy and precision of estimates of DNA concentration are critical factors for efficient use of DNA samples in high-throughput genotype and sequence analyses. We evaluated the performance of spectrophot...

    Authors: Kashif A Haque, Ruth M Pfeiffer, Michael B Beerman, Jeff P Struewing, Stephen J Chanock and Andrew W Bergen

    Citation: BMC Biotechnology 2003 3:20

    Content type: Methodology article

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  47. C. elegans has been established as a powerful genetic system. Use of a chemically defined medium (C. elegans Maintenance Medium (CeMM)) now allows standardization and systematic manipulation of the nutrients that...

    Authors: Nathaniel J Szewczyk, Elena Kozak and Catharine A Conley

    Citation: BMC Biotechnology 2003 3:19

    Content type: Methodology article

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  48. Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR produc...

    Authors: Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs and Alex F Markham

    Citation: BMC Biotechnology 2003 3:18

    Content type: Methodology article

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  49. Expression of human Interleukin-5 receptor alpha (hIL-5Rα) is controlled by alternative splicing, which generates two different transcripts encoding a membrane-anchored and a soluble form of the receptor, resp...

    Authors: Claudina Pérez, Jo Vandesompele, Ina Vandenbroucke, Gabriele Holtappels, Frank Speleman, Philippe Gevaert, Paul Van cauwenberge and Claus Bachert

    Citation: BMC Biotechnology 2003 3:17

    Content type: Methodology article

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  50. Gene transfer into the amniotic fluid using recombinant adenovirus vectors was shown previously to result in high efficiency transfer of transgenes into the lungs and intestines. Adenovirus mediated in utero gene...

    Authors: Deiadra J Garrett, Janet E Larson, Daisy Dunn, Luis Marrero and J Craig Cohen

    Citation: BMC Biotechnology 2003 3:16

    Content type: Methodology article

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