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  1. Optical imaging is an attractive non-invasive way to evaluate the expression of a transferred DNA, mainly thanks to its lower cost and ease of realization. In this study optical imaging was evaluated for monit...

    Authors: C Bloquel, C Trollet, E Pradines, J Seguin, D Scherman and MF Bureau

    Citation: BMC Biotechnology 2006 6:16

    Content type: Research article

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  2. The identification of protein trafficking signals, and their interacting mechanisms, is a fundamental objective of modern biology. Unfortunately, the analysis of trafficking signals is complicated by their top...

    Authors: Lynn McKeown, Philip Robinson, Sam M Greenwood, Weiwen Hu and Owen T Jones

    Citation: BMC Biotechnology 2006 6:15

    Content type: Methodology article

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  3. The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of ...

    Authors: Kurt J Langenbach, John T Elliott, Alex Tona, Dennis McDaniel and Anne L Plant

    Citation: BMC Biotechnology 2006 6:14

    Content type: Methodology article

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  4. Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In th...

    Authors: Karen Colwill, Clark D Wells, Kelly Elder, Marilyn Goudreault, Kadija Hersi, Sarang Kulkarni, W Rod Hardy, Tony Pawson and Gregg B Morin

    Citation: BMC Biotechnology 2006 6:13

    Content type: Methodology article

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  5. In the past few years, both automated and manual high-throughput protein expression and purification has become an accessible means to rapidly screen and produce soluble proteins for structural and functional ...

    Authors: Lisa D Cabrita, Weiwen Dai and Stephen P Bottomley

    Citation: BMC Biotechnology 2006 6:12

    Content type: Methodology article

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  6. Adenovirus (Ad) mediated gene transfer is a well-established tool to transiently express constructs in livers of mice in vivo. In the present study, we determined the specificity and efficiency of Ad vectors expr...

    Authors: Yvonne D Krom, Frits J Fallaux, Ivo Que, Clemens Lowik and Ko Willems van Dijk

    Citation: BMC Biotechnology 2006 6:11

    Content type: Research article

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  7. The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (β2GPI) is thought to co...

    Authors: Yiannis Ioannou, Ian Giles, Anastasia Lambrianides, Chris Richardson, Laurence H Pearl, David S Latchman, David A Isenberg and Anisur Rahman

    Citation: BMC Biotechnology 2006 6:8

    Content type: Methodology article

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  8. RNA interference (RNAi) technology is a powerful methodology recently developed for the specific knockdown of targeted genes. RNAi is most commonly achieved either transiently by transfection of small interfer...

    Authors: Debra J Taxman, Laura R Livingstone, Jinghua Zhang, Brian J Conti, Heather A Iocca, Kristi L Williams, John D Lich, Jenny P-Y Ting and William Reed

    Citation: BMC Biotechnology 2006 6:7

    Content type: Methodology article

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  9. Haplosclerid marine sponges produce pore forming polyalkylpyridinium salts (poly-APS), which can be used to deliver macromolecules into cells. The aim of this study was to investigate the delivery of DNA, siRN...

    Authors: Debra McLaggan, Noppadon Adjimatera, Kristina Sepčić, Marcel Jaspars, David J MacEwan, Ian S Blagbrough and Roderick H Scott

    Citation: BMC Biotechnology 2006 6:6

    Content type: Research article

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  10. We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing bec...

    Authors: Daniel Gendron, Sandra Carriero, Daniel Garneau, Jonathan Villemaire, Roscoe Klinck, Sherif Abou Elela, Masad J Damha and Benoit Chabot

    Citation: BMC Biotechnology 2006 6:5

    Content type: Methodology article

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  11. Internal Ribosome Entry Site (IRES)-based bicistronic vectors are important tools in today's cell biology. Among applications, the expression of two proteins under the control of a unique promoter permits the ...

    Authors: Patrick Martin, Olivier Albagli, Marie Christine Poggi, Kim E Boulukos and Philippe Pognonec

    Citation: BMC Biotechnology 2006 6:4

    Content type: Methodology article

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  12. The use of lactic acid bacteria as vehicles to delivery antigens to immunize animals is a promising issue. When genetically modified, these bacteria can induce a specific local and systemic immune response aga...

    Authors: Rodrigo M Mota, João Luiz S Moreira, Marcelo R Souza, M Fátima Horta, Santuza MR Teixeira, Elisabeth Neumann, Jacques R Nicoli and Álvaro C Nunes

    Citation: BMC Biotechnology 2006 6:2

    Content type: Research article

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  13. The isozymes of alkaline phosphatase, the tissue non-specific, intestinal and placental, have similar properties and a high degree of identity. The placental isozyme (PLAP) is an oncofetal antigen expressed in...

    Authors: Deepti Saini, Mrinalini Kala, Vishal Jain and Subrata Sinha

    Citation: BMC Biotechnology 2005 5:33

    Content type: Research article

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  14. Parthenocarpic tomato lines transgenic for the DefH9-RI-iaaM gene have been cultivated under open field conditions to address some aspects of the equivalence of genetically modified (GM) fruit in comparison to co...

    Authors: Giuseppe Leonardo Rotino, Nazareno Acciarri, Emidio Sabatini, Giuseppe Mennella, Roberto Lo Scalzo, Andrea Maestrelli, Barbara Molesini, Tiziana Pandolfini, Jessica Scalzo, Bruno Mezzetti and Angelo Spena

    Citation: BMC Biotechnology 2005 5:32

    Content type: Research article

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  15. As real-time quantitative PCR (RT-QPCR) is increasingly being relied upon for the enforcement of legislation and regulations dependent upon the trace detection of DNA, focus has increased on the quality issues...

    Authors: Malcolm J Burns, Gavin J Nixon, Carole A Foy and Neil Harris

    Citation: BMC Biotechnology 2005 5:31

    Content type: Research article

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  16. Efficient targeting to appropriate cell organelles is one of the bottlenecks for the production of recombinant proteins in plant systems. A common practice is to use the native secretory signal peptide of the ...

    Authors: Andreas Schaaf, Stefanie Tintelnot, Armin Baur, Ralf Reski, Gilbert Gorr and Eva L Decker

    Citation: BMC Biotechnology 2005 5:30

    Content type: Research article

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  17. The chicken avidin gene family consists of avidin and several avidin related genes (AVRs). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a pro...

    Authors: Vesa P Hytönen, Juha AE Määttä, Heidi Kidron, Katrin K Halling, Jarno Hörhä, Tuomas Kulomaa, Thomas KM Nyholm, Mark S Johnson, Tiina A Salminen, Markku S Kulomaa and Tomi T Airenne

    Citation: BMC Biotechnology 2005 5:28

    Content type: Research article

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  18. Precise analysis of expression-regulating elements, such as enhancers and insulators, requires that they be tested under reproducible, isogenic conditions. The commonly used methods of transfecting DNA into ce...

    Authors: Emily Y Cheng, Cathy Collins, Maribel Berru and Marc J Shulman

    Citation: BMC Biotechnology 2005 5:27

    Content type: Methodology article

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  19. The definite diagnosis of prion diseases such as Creutzfeldt-Jakob disease (CJD) in humans or bovine spongiform encephalopathy (BSE) in cattle currently relies on the post mortem detection of the pathological for...

    Authors: Lothar Trieschmann, Alexander Navarrete Santos, Katja Kaschig, Sandra Torkler, Elke Maas, Hermann Schätzl and Gerald Böhm

    Citation: BMC Biotechnology 2005 5:26

    Content type: Research article

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  20. Whole genome amplification (WGA) promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA (gDNA) quantity. We evaluated the performance of multiple displacement amplif...

    Authors: Andrew W Bergen, Ying Qi, Kashif A Haque, Robert A Welch and Stephen J Chanock

    Citation: BMC Biotechnology 2005 5:24

    Content type: Methodology article

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  21. Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restricti...

    Authors: Menachem Ailenberg, Neil M Goldenberg and Mel Silverman

    Citation: BMC Biotechnology 2005 5:23

    Content type: Methodology article

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  22. Assays detecting human antigen-specific antibodies are medically useful. However, the usefulness of existing simple immunoassay formats is limited by technical considerations such as sera antibodies to contami...

    Authors: Peter D Burbelo, Radoslav Goldman and Thomas L Mattson

    Citation: BMC Biotechnology 2005 5:22

    Content type: Methodology article

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  23. Human Artificial Chromosomes (HACs) are potentially useful vectors for gene transfer studies and for functional annotation of the genome because of their suitability for cloning, manipulating and transferring ...

    Authors: Joydeep Basu, George Compitello, Gregory Stromberg, Huntington F Willard and Gil Van Bokkelen

    Citation: BMC Biotechnology 2005 5:21

    Content type: Research article

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  24. DsRed the red fluorescent protein (RFP) isolated from Discosoma sp. coral holds much promise as a genetically and spectrally distinct alternative to green fluorescent protein (GFP) for application in mice. Widesp...

    Authors: Jonathan Z Long, Chantal S Lackan and Anna-Katerina Hadjantonakis

    Citation: BMC Biotechnology 2005 5:20

    Content type: Methodology article

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  25. The expression of recombinant proteins in eukaryotic cells requires the fusion of the coding region to a promoter functional in the eukaryotic cell line. Viral promoters are very often used for this purpose. T...

    Authors: Astrid Lewin, Martin Mayer, Janet Chusainow, Daniela Jacob and Bernd Appel

    Citation: BMC Biotechnology 2005 5:19

    Content type: Research article

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  26. Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contras...

    Authors: Victoria Yamazaki, Oksana Sirenko, Robert J Schafer, Luat Nguyen, Thomas Gutsmann, Lore Brade and Jay T Groves

    Citation: BMC Biotechnology 2005 5:18

    Content type: Methodology article

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  27. The hCMV promoter is very commonly used for high level expression of transgenes in mammalian cells, but its utility is hindered by transcriptional silencing. Large genomic fragments incorporating the CpG island r...

    Authors: Steven Williams, Tracey Mustoe, Tony Mulcahy, Mark Griffiths, David Simpson, Michael Antoniou, Alistair Irvine, Andrew Mountain and Robert Crombie

    Citation: BMC Biotechnology 2005 5:17

    Content type: Research article

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  28. High-throughput screening is used by the pharmaceutical industry for identifying lead compounds that interact with targets of pharmacological interest. Because of the key role that aberrant regulation of prote...

    Authors: Frauke Rininsland, Casey Stankewicz, Wendy Weatherford and Duncan McBranch

    Citation: BMC Biotechnology 2005 5:16

    Content type: Methodology article

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  29. The wide variety of real-time amplification platforms currently available has determined that standardisation of DNA measurements is a fundamental aspect involved in the comparability of results.

    Authors: Carol E Donald, Fizza Qureshi, Malcolm J Burns, Marcia J Holden, Joseph R Blasic Jr and Alison J Woolford

    Citation: BMC Biotechnology 2005 5:15

    Content type: Research article

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  30. The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types ...

    Authors: Erik Sahai, Jeffrey Wyckoff, Ulrike Philippar, Jeffrey E Segall, Frank Gertler and John Condeelis

    Citation: BMC Biotechnology 2005 5:14

    Content type: Methodology article

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  31. The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-b...

    Authors: Luke S Lambeth, Robert J Moore, Morley Muralitharan, Brian P Dalrymple, Sean McWilliam and Timothy J Doran

    Citation: BMC Biotechnology 2005 5:13

    Content type: Research article

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  32. Analyzing apoptosis has been an integral component of many biological studies. However, currently available methods for quantifying apoptosis have various limitations including multiple, sometimes cell-damagin...

    Authors: Deborah Ribble, Nathaniel B Goldstein, David A Norris and Yiqun G Shellman

    Citation: BMC Biotechnology 2005 5:12

    Content type: Methodology article

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  33. Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a signi...

    Authors: Tristan Ruysschaert, Audrey Marque, Jean-Luc Duteyrat, Sylviane Lesieur, Mathias Winterhalter and Didier Fournier

    Citation: BMC Biotechnology 2005 5:11

    Content type: Methodology article

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  34. Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.

    Authors: Frédéric R Raymond, Hoang-Anh Ho, Régis Peytavi, Luc Bissonnette, Maurice Boissinot, François J Picard, Mario Leclerc and Michel G Bergeron

    Citation: BMC Biotechnology 2005 5:10

    Content type: Methodology article

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  35. Uromodulin is the most abundant protein found in the urine of mammals. In an effort to utilize the uromodulin promoter in order to target recombinant proteins in the urine of transgenic animals we have cloned ...

    Authors: Yue-Jin Huang, Nathalie Chretien, Annie S Bilodeau, Jiang Feng Zhou, Anthoula Lazaris and Costas N Karatzas

    Citation: BMC Biotechnology 2005 5:9

    Content type: Research article

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  36. We have developed an oligonucleotide microarray (genosensor) utilizing a double tandem hybridization technique to search for 9 point mutations located in the most frequently altered codons of the TP53 gene. Isola...

    Authors: Angélica Rangel-López, Rogelio Maldonado-Rodríguez, Mauricio Salcedo-Vargas, Juana Mercedes Espinosa-Lara, Alfonso Méndez-Tenorio and Kenneth L Beattie

    Citation: BMC Biotechnology 2005 5:8

    Content type: Methodology article

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  37. The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic s...

    Authors: Sreelatha Meleth, Jessy Deshane and Helen Kim

    Citation: BMC Biotechnology 2005 5:7

    Content type: Research article

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  38. Transcriptional profiling using microarrays has developed into a key molecular tool for the elucidation of gene function and gene regulation. Microarray platforms based on either oligonucleotides or purified a...

    Authors: Valtteri Wirta, Anders Holmberg, Morten Lukacs, Peter Nilsson, Pierre Hilson, Mathias Uhlén, Rishikesh P Bhalerao and Joakim Lundeberg

    Citation: BMC Biotechnology 2005 5:5

    Content type: Methodology article

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  39. Common oral diseases and dental caries can be prevented effectively by passive immunization. In humans, passive immunotherapy may require the use of humanized or human antibodies to prevent adverse immune resp...

    Authors: Michael B Kupper, Michael Huhn, Holger Spiegel, Julian KC Ma, Stefan Barth, Rainer Fischer and Ricarda Finnern

    Citation: BMC Biotechnology 2005 5:4

    Content type: Research article

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  40. In the past ten years there has been a growing interest in engineering Gram-positive bacteria for biotechnological applications, including vaccine delivery and production of recombinant proteins. Usually, bact...

    Authors: Roberta Provvedi, Tiziana Maggi, Marco R Oggioni, Riccardo Manganelli and Gianni Pozzi

    Citation: BMC Biotechnology 2005 5:3

    Content type: Methodology article

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  41. Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is part...

    Authors: Cristina Hartshorn, Aleksandra Anshelevich and Lawrence J Wangh

    Citation: BMC Biotechnology 2005 5:2

    Content type: Methodology article

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  42. Recombinant adenoviral vectors are highly efficient for in vitro and in vivo gene delivery. They can easily be produced in large numbers, transduce a wide variety of cell types and generate high levels of tran...

    Authors: Dalibor Antolovic, Moritz Koch, Inga Bohlmann, Peter Kienle, Markus Büchler and Jürgen Weitz

    Citation: BMC Biotechnology 2005 5:1

    Content type: Methodology article

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