Skip to content



Page 28 of 29

  1. Content type: Research article

    Members of the GATA transcription factor family have been used in many transfection studies to investigate their roles in the regulation of gene expression. To correct for variations in transfection efficiency...

    Authors: Clement KM Ho and Jerome F Strauss III

    Citation: BMC Biotechnology 2004 4:10

    Published on:

  2. Content type: Methodology article

    Whether for cell culture studies of protein function, construction of mouse models to enable in vivo analysis of disease epidemiology, or ultimately gene therapy of human diseases, a critical enabling step is the...

    Authors: Marina Sudomoina, Ekaterina Latypova, Olga O Favorova, Erica A Golemis and Ilya G Serebriiskii

    Citation: BMC Biotechnology 2004 4:9

    Published on:

  3. Content type: Methodology article

    Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either g...

    Authors: Susanna Harju, Halyna Fedosyuk and Kenneth R Peterson

    Citation: BMC Biotechnology 2004 4:8

    Published on:

  4. Content type: Research article

    Antibody fragments selected from large combinatorial libraries have numerous applications in diagnosis and therapy. Most existing antibody repertoires are derived from human immunoglobulin genes. Genes from ot...

    Authors: Wouter van Wyngaardt, Teresiah Malatji, Cordelia Mashau, Jeanni Fehrsen, Frances Jordaan, Dubravka Miltiadou and Dion H du Plessis

    Citation: BMC Biotechnology 2004 4:6

    Published on:

  5. Content type: Methodology article

    Primary hepatocytes, one of the most widely used cell types for toxicological studies, have a very limited life span and must be freshly derived from mice or even humans. Attempts to use stable cell lines main...

    Authors: Maria Grazia Sacco, Laura Amicone, Enrica Mira Catò, Daniela Filippini, Paolo Vezzoni and Marco Tripodi

    Citation: BMC Biotechnology 2004 4:5

    Published on:

  6. Content type: Research article

    The DefH9-iaaM gene fusion which is expressed specifically in placenta/ovules and promotes auxin-synthesis confers parthenocarpic fruit development to eggplant, tomato and tobacco. Transgenic DefH9-iaaM eggplants...

    Authors: Bruno Mezzetti, Lucia Landi, Tiziana Pandolfini and Angelo Spena

    Citation: BMC Biotechnology 2004 4:4

    Published on:

  7. Content type: Methodology article

    DNA microarray assays typically compare two biological samples and present the results of those comparisons gene-by-gene as the logarithm base two of the ratio of the measured expression levels for the two sam...

    Authors: Vasily Sharov, Ka Yin Kwong, Bryan Frank, Emily Chen, Jeremy Hasseman, Renee Gaspard, Yan Yu, Ivana Yang and John Quackenbush

    Citation: BMC Biotechnology 2004 4:3

    Published on:

  8. Content type: Methodology article

    Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by...

    Authors: Rajiv Tyagi, Richard Lai and Ronald G Duggleby

    Citation: BMC Biotechnology 2004 4:2

    Published on:

  9. Content type: Methodology article

    BAC clones containing entire mammalian genes including all the transcribed region and long range controlling elements are very useful for functional analysis. Sequenced BACs are available for most of the human...

    Authors: George Kotzamanis and Clare Huxley

    Citation: BMC Biotechnology 2004 4:1

    Published on:

  10. Content type: Methodology article

    Several different cDNA labeling methods have been developed for microarray based gene expression analysis. We have examined the accuracy and reproducibility of such five commercially available methods in detec...

    Authors: Azadeh Badiee, Hans Geir Eiken, Vidar M Steen and Roger Løvlie

    Citation: BMC Biotechnology 2003 3:23

    Published on:

  11. Content type: Methodology article

    The accuracy and precision of estimates of DNA concentration are critical factors for efficient use of DNA samples in high-throughput genotype and sequence analyses. We evaluated the performance of spectrophot...

    Authors: Kashif A Haque, Ruth M Pfeiffer, Michael B Beerman, Jeff P Struewing, Stephen J Chanock and Andrew W Bergen

    Citation: BMC Biotechnology 2003 3:20

    Published on:

  12. Content type: Methodology article

    C. elegans has been established as a powerful genetic system. Use of a chemically defined medium (C. elegans Maintenance Medium (CeMM)) now allows standardization and systematic manipulation of the nutrients that...

    Authors: Nathaniel J Szewczyk, Elena Kozak and Catharine A Conley

    Citation: BMC Biotechnology 2003 3:19

    Published on:

  13. Content type: Methodology article

    Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR produc...

    Authors: Frederique Ponchel, Carmel Toomes, Kieran Bransfield, Fong T Leong, Susan H Douglas, Sarah L Field, Sandra M Bell, Valerie Combaret, Alain Puisieux, Alan J Mighell, Philip A Robinson, Chris F Inglehearn, John D Isaacs and Alex F Markham

    Citation: BMC Biotechnology 2003 3:18

    Published on:

  14. Content type: Methodology article

    Expression of human Interleukin-5 receptor alpha (hIL-5Rα) is controlled by alternative splicing, which generates two different transcripts encoding a membrane-anchored and a soluble form of the receptor, resp...

    Authors: Claudina Pérez, Jo Vandesompele, Ina Vandenbroucke, Gabriele Holtappels, Frank Speleman, Philippe Gevaert, Paul Van cauwenberge and Claus Bachert

    Citation: BMC Biotechnology 2003 3:17

    Published on:

  15. Content type: Methodology article

    Gene transfer into the amniotic fluid using recombinant adenovirus vectors was shown previously to result in high efficiency transfer of transgenes into the lungs and intestines. Adenovirus mediated in utero gene...

    Authors: Deiadra J Garrett, Janet E Larson, Daisy Dunn, Luis Marrero and J Craig Cohen

    Citation: BMC Biotechnology 2003 3:16

    Published on:

  16. Content type: Research article

    Interleukin-1 (IL-1) is a cytokine involved in the initiation and amplification of the defence response in infectious and inflammatory diseases. IL-1 receptor antagonist (IL-1ra) is an inactive member of the I...

    Authors: Susanna Ricci, Giovanni Macchia, Paolo Ruggiero, Tiziana Maggi, Paola Bossù, Li Xu, Donata Medaglini, Aldo Tagliabue, Lennart Hammarström, Gianni Pozzi and Diana Boraschi

    Citation: BMC Biotechnology 2003 3:15

    Published on:

  17. Content type: Research article

    Non-viral vectors for gene transfer are less immunogenic than viral vectors but also less efficient. Significant effort has focused on enhancing non-viral gene transfer efficiency by increasing nuclear import ...

    Authors: Marie Carrière, Virginie Escriou, Aline Savarin and Daniel Scherman

    Citation: BMC Biotechnology 2003 3:14

    Published on:

  18. Content type: Methodology article

    cDNA microarrays have the potential to identify the genes involved in invasion and metastasis. However, when used with whole tumor tissue, the results average the expression patterns of different cell types. W...

    Authors: Weigang Wang, Jeffrey B Wyckoff, Yarong Wang, Erwin P Bottinger, Jeffrey E Segall and John S Condeelis

    Citation: BMC Biotechnology 2003 3:13

    Published on:

  19. Content type: Methodology article

    Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these pro...

    Authors: Christoph Scheich, Volker Sievert and Konrad Büssow

    Citation: BMC Biotechnology 2003 3:12

    Published on:

  20. Content type: Methodology article

    The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as po...

    Authors: James Butz, Eric Wickstrom and Jeremy Edwards

    Citation: BMC Biotechnology 2003 3:11

    Published on:

  21. Content type: Methodology article

    The interest in microfluidics and surface patterning is increasing as the use of these technologies in diverse biomedical applications is substantiated. Controlled molecular and cellular surface patterning is ...

    Authors: Thomas Brevig, Ulrich Krühne, Rachel A Kahn, Thomas Ahl, Michael Beyer and Lars H Pedersen

    Citation: BMC Biotechnology 2003 3:10

    Published on:

  22. Content type: Research article

    Homology-dependent selective degradation of RNA, or post-transcriptional gene silencing (PTGS), is involved in several biological phenomena, including adaptative defense mechanisms against plant viruses. Small...

    Authors: Tiziana Pandolfini, Barbara Molesini, Linda Avesani, Angelo Spena and Annalisa Polverari

    Citation: BMC Biotechnology 2003 3:7

    Published on:

  23. Content type: Research article

    DNA microarrays are now routinely used to monitor the transcript levels of thousands of genes simultaneously. However, the array fabrication method, hybridization conditions, and oligodeoxyribonucleotide probe...

    Authors: David R Dorris, Allen Nguyen, Linn Gieser, Randall Lockner, Anna Lublinsky, Marcus Patterson, Edward Touma, Timothy J Sendera, Robert Elghanian and Abhijit Mazumder

    Citation: BMC Biotechnology 2003 3:6

    Published on:

  24. Content type: Research article

    Double-stranded RNA (dsRNA) is a potent initiator of gene silencing in a diverse group of organisms that includes plants, Caenorhabditis elegans, Drosophila and mammals. We have previously shown and patented that...

    Authors: Francisco Tenllado, Belén Martínez-García, Marisol Vargas and José Ramón Díaz-Ruíz

    Citation: BMC Biotechnology 2003 3:3

    Published on:

  25. Content type: Research article

    Bacterial artificial chromosomes (BACs) have been used extensively for sequencing the human and mouse genomes and are thus readily available for most genes. The large size of BACs means that they can generally...

    Authors: Christine Magin-Lachmann, George Kotzamanis, Leonardo D'Aiuto, Ernst Wagner and Clare Huxley

    Citation: BMC Biotechnology 2003 3:2

    Published on:

  26. Content type: Methodology article

    The ability to manipulate the genetic networks underlying the physiological and behavioural repertoires of the adult honeybee worker (Apis mellifera) is likely to deepen our understanding of issues such as learni...

    Authors: Gro V Amdam, Zilá LP Simões, Karina R Guidugli, Kari Norberg and Stig W Omholt

    Citation: BMC Biotechnology 2003 3:1

    Published on:

  27. Content type: Research article

    Allosteric ribozymes (aptazymes) that have extraordinary activation parameters have been generated in vitro by design and selection. For example, hammerhead and ligase ribozymes that are activated by small organi...

    Authors: Kristin M Thompson, Heather A Syrett, Scott M Knudsen and Andrew D Ellington

    Citation: BMC Biotechnology 2002 2:21

    Published on:

  28. Content type: Methodology article

    When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this...

    Authors: Giovanna Mason, Paolo Provero, Anna Maria Vaira and Gian Paolo Accotto

    Citation: BMC Biotechnology 2002 2:20

    Published on:

  29. Content type: Methodology article

    Ostrich (Struthio camelus) breeds have been gaining increasing significance around the world. The large-scale sex determination of chicks is an important task in the development of these breeds. To date, two PCR-...

    Authors: Wilson Malagó Jr, Heitor M Franco, Euclides Matheucci Jr, Adriana Medaglia and Flavio Henrique-Silva

    Citation: BMC Biotechnology 2002 2:19

    Published on:

  30. Content type: Research article

    Efficient transformation and regeneration methods are a priority for successful application of genetic engineering to vegetative propagated plants such as grape. The current methods for the production of trans...

    Authors: Bruno Mezzetti, Tiziana Pandolfini, Oriano Navacchi and Lucia Landi

    Citation: BMC Biotechnology 2002 2:18

    Published on:

  31. Content type: Methodology article

    The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. It has also been suggested for screening pharmacological activities in plant extracts. However, we think tha...

    Authors: José Luis Carballo, Zaira L Hernández-Inda, Pilar Pérez and María D García-Grávalos

    Citation: BMC Biotechnology 2002 2:17

    Published on:

  32. Content type: Research article

    Hep27 monoclonal (Hep27 Mab) is an antibody against hepatocellular carcinoma. Hep27 Mab itself can inhibit the growth of a hepatocellular carcinoma cell line (HCC-S102). We attempted to produce a single-chain ...

    Authors: Duanpen Sandee, Sumalee Tungpradabkul, Manae Tsukio, Tadayuki Imanaka and Masahiro Takagi

    Citation: BMC Biotechnology 2002 2:16

    Published on:

  33. Content type: Methodology article

    The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high produc...

    Authors: Eric Devroe and Pamela A Silver

    Citation: BMC Biotechnology 2002 2:15

    Published on:

  34. Content type: Research article

    The power of DNA microarrays derives from their ability to monitor the expression levels of many genes in parallel. One of the limitations of such powerful analytical tools is the inability to detect certain t...

    Authors: Allen Nguyen, Connie Zhao, David Dorris and Abhijit Mazumder

    Citation: BMC Biotechnology 2002 2:14

    Published on:

  35. Content type: Research article

    In a variant of the standard PCR reaction termed bridging, or jumping, PCR the primer-bound sequences are originally on separate template molecules. Bridging can occur if, and only if, the templates contain a ...

    Authors: Shumo Liu, David S Thaler and Albert Libchaber

    Citation: BMC Biotechnology 2002 2:13

    Published on:

  36. Content type: Research article

    Cationic lipid DNA complexes based on DOTAP (1,2-dioleoyl-3-(trimethyammonium) propane) and mixtures of DOTAP and cholesterol (DC) have been previously optimized for transfection efficiency in the absence of s...

    Authors: Godwin Nchinda, Klaus Überla and Olaf Zschörnig

    Citation: BMC Biotechnology 2002 2:12

    Published on:

  37. Content type: Methodology article

    Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria ...

    Authors: Anna-Katerina Hadjantonakis, Suzanne Macmaster and Andras Nagy

    Citation: BMC Biotechnology 2002 2:11

    Published on:

  38. Content type: Methodology article

    This study tested a low-volume (20–30 μl/20–30 μg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation o...

    Authors: Shuxun Ren, Minglin Li, Joanne M Smith, Louis J DeTolla and Priscilla A Furth

    Citation: BMC Biotechnology 2002 2:10

    Published on:

  39. Content type: Methodology article

    We investigated the encapsulation mechanism of enzymes into liposomes. The existing protocols to achieve high encapsulation efficiencies are basically optimized for chemically stable molecules. Enzymes, howeve...

    Authors: Jacques-Philippe Colletier, Barnabé Chaize, Mathias Winterhalter and Didier Fournier

    Citation: BMC Biotechnology 2002 2:9

    Published on:

  40. Content type: Methodology article

    Determination of clot lysis times on whole blood, diluted whole blood, plasma or plasma fraction has been used for many years to assess the overall activity of the fibrinolytic system. We designed a completely...

    Authors: K Zouaoui Boudjeltia, Ph Cauchie, Cl Remacle, M Guillaume, D Brohée, JL Hubert and M Vanhaeverbeek

    Citation: BMC Biotechnology 2002 2:8

    Published on:

  41. Content type: Research article

    Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limi...

    Authors: Keejong Chang, Jin Qian, MeiSheng Jiang, Yi-Hsin Liu, Ming-Che Wu, Chi-Dar Chen, Chao-Kuen Lai, Hsin-Lung Lo, Chin-Ton Hsiao, Lucy Brown, James Bolen Jr, Hsiao-I Huang, Pei-Yu Ho, Ping Yao Shih, Chen-Wen Yao, Wey-Jinq Lin…

    Citation: BMC Biotechnology 2002 2:5

    Published on: