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  1. Content type: Research article

    Efficient transformation and regeneration methods are a priority for successful application of genetic engineering to vegetative propagated plants such as grape. The current methods for the production of trans...

    Authors: Bruno Mezzetti, Tiziana Pandolfini, Oriano Navacchi and Lucia Landi

    Citation: BMC Biotechnology 2002 2:18

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  2. Content type: Methodology article

    The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. It has also been suggested for screening pharmacological activities in plant extracts. However, we think tha...

    Authors: José Luis Carballo, Zaira L Hernández-Inda, Pilar Pérez and María D García-Grávalos

    Citation: BMC Biotechnology 2002 2:17

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  3. Content type: Research article

    Hep27 monoclonal (Hep27 Mab) is an antibody against hepatocellular carcinoma. Hep27 Mab itself can inhibit the growth of a hepatocellular carcinoma cell line (HCC-S102). We attempted to produce a single-chain ...

    Authors: Duanpen Sandee, Sumalee Tungpradabkul, Manae Tsukio, Tadayuki Imanaka and Masahiro Takagi

    Citation: BMC Biotechnology 2002 2:16

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  4. Content type: Methodology article

    The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high produc...

    Authors: Eric Devroe and Pamela A Silver

    Citation: BMC Biotechnology 2002 2:15

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  5. Content type: Research article

    The power of DNA microarrays derives from their ability to monitor the expression levels of many genes in parallel. One of the limitations of such powerful analytical tools is the inability to detect certain t...

    Authors: Allen Nguyen, Connie Zhao, David Dorris and Abhijit Mazumder

    Citation: BMC Biotechnology 2002 2:14

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  6. Content type: Research article

    In a variant of the standard PCR reaction termed bridging, or jumping, PCR the primer-bound sequences are originally on separate template molecules. Bridging can occur if, and only if, the templates contain a ...

    Authors: Shumo Liu, David S Thaler and Albert Libchaber

    Citation: BMC Biotechnology 2002 2:13

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  7. Content type: Research article

    Cationic lipid DNA complexes based on DOTAP (1,2-dioleoyl-3-(trimethyammonium) propane) and mixtures of DOTAP and cholesterol (DC) have been previously optimized for transfection efficiency in the absence of s...

    Authors: Godwin Nchinda, Klaus Überla and Olaf Zschörnig

    Citation: BMC Biotechnology 2002 2:12

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  8. Content type: Methodology article

    Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria ...

    Authors: Anna-Katerina Hadjantonakis, Suzanne Macmaster and Andras Nagy

    Citation: BMC Biotechnology 2002 2:11

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  9. Content type: Methodology article

    This study tested a low-volume (20–30 μl/20–30 μg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation o...

    Authors: Shuxun Ren, Minglin Li, Joanne M Smith, Louis J DeTolla and Priscilla A Furth

    Citation: BMC Biotechnology 2002 2:10

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  10. Content type: Methodology article

    We investigated the encapsulation mechanism of enzymes into liposomes. The existing protocols to achieve high encapsulation efficiencies are basically optimized for chemically stable molecules. Enzymes, howeve...

    Authors: Jacques-Philippe Colletier, Barnabé Chaize, Mathias Winterhalter and Didier Fournier

    Citation: BMC Biotechnology 2002 2:9

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  11. Content type: Methodology article

    Determination of clot lysis times on whole blood, diluted whole blood, plasma or plasma fraction has been used for many years to assess the overall activity of the fibrinolytic system. We designed a completely...

    Authors: K Zouaoui Boudjeltia, Ph Cauchie, Cl Remacle, M Guillaume, D Brohée, JL Hubert and M Vanhaeverbeek

    Citation: BMC Biotechnology 2002 2:8

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  12. Content type: Research article

    Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limi...

    Authors: Keejong Chang, Jin Qian, MeiSheng Jiang, Yi-Hsin Liu, Ming-Che Wu, Chi-Dar Chen, Chao-Kuen Lai, Hsin-Lung Lo, Chin-Ton Hsiao, Lucy Brown, James Bolen Jr, Hsiao-I Huang, Pei-Yu Ho, Ping Yao Shih, Chen-Wen Yao, Wey-Jinq Lin…

    Citation: BMC Biotechnology 2002 2:5

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  13. Content type: Research article

    Parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the DefH9-iaaM gene. The iaaM gene codes for tryptophan...

    Authors: Nazzareno Acciarri, Federico Restaino, Gabriele Vitelli, Domenico Perrone, Michela Zottini, Tiziana Pandolfini, Angelo Spena and Giuseppe Leonardo Rotino

    Citation: BMC Biotechnology 2002 2:4

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  14. Content type: Research article

    The suitability of the strain Rhodococcus erythropolis ATCC 25544 grown in a two-liter fermentor as a source of cholesterol oxidase has been investigated. The strain produces both cell-linked and extracellular ch...

    Authors: Mar M Sojo, Roque R Bru and Francisco F García-Carmona

    Citation: BMC Biotechnology 2002 2:3

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  15. Content type: Research article

    Agrobacterium vitis is a causal agent of crown-gall disease. Trifolitoxin (TFX) is a peptide antibiotic active only against members of a specific group of α-proteobacteria that includes Agrobacterium and its clos...

    Authors: Thomas C Herlache and Eric W Triplett

    Citation: BMC Biotechnology 2002 2:2

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  16. Content type: Methodology article

    Genetic engineering of parthenocarpy confers to horticultural plants the ability to produce fruits under environmental conditions that curtail fruit productivity and quality. The DefH9-iaaM transgene, whose predi...

    Authors: Tiziana Pandolfini, Giuseppe L Rotino, Serena Camerini, Roberto Defez and Angelo Spena

    Citation: BMC Biotechnology 2002 2:1

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  17. Content type: Research article

    The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its promoter. A combined in vitro / in vivo sele...

    Authors: Jijumon Chelliserrykattil, George Cai and Andrew D Ellington

    Citation: BMC Biotechnology 2001 1:13

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  18. Content type: Research article

    Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and ...

    Authors: Larry R Karns PhD, Anne Kisielewski BSc, Kathryn M Gulding BSc and Dan Theodorescu MD PhD

    Citation: BMC Biotechnology 2001 1:11

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  19. Content type: Methodology article

    Transgenic mice have been used extensively to analyze gene function. Unfortunately, traditional transgenic procedures have only limited use in analyzing alleles that cause lethality because lines of founder mi...

    Authors: Ravi P Misra, Sarah K Bronson, Qi Xiao, Wendy Garrison, Jixuan Li, Roong Zhao and Stephen A Duncan

    Citation: BMC Biotechnology 2001 1:12

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  20. Content type: Methodology article

    To maintain populations of microbial cells under controlled conditions of growth and environment for an indefinite duration is a prerequisite for experimentally evolving natural isolates of wild-type species o...

    Authors: Valérie A de Crécy-Lagard, Jacques Bellalou, Rupert Mutzel and Philippe Marlière

    Citation: BMC Biotechnology 2001 1:10

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  21. Content type: Research article

    Modern drug discovery is concerned with identification and validation of novel protein targets from among the 30,000 genes or more postulated to be present in the human genome. While protein-protein interactio...

    Authors: Renuka C Pillutla, Ku-chuan Hsiao, Renee Brissette, Paul S Eder, Tony Giordano, Paul W Fletcher, Michael Lennick, Arthur J Blume and Neil I Goldstein

    Citation: BMC Biotechnology 2001 1:6

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  22. Content type: Research article

    There have been many attempts to develop new materials with stability and high affinity towards immunoglobulins. Some of glycolipids such as gangliosides exhibit a high affinity toward immunoglobulins. However...

    Authors: Jae Hong Im, Takashi Nakane, Hiroshi Yanagishita, Toru Ikegami and Dai Kitamoto

    Citation: BMC Biotechnology 2001 1:5

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  23. Content type: Research article

    Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromol...

    Authors: Vadim V Shmanai, Tamara A Nikolayeva, Ludmila G Vinokurova and Anatoli A Litoshka

    Citation: BMC Biotechnology 2001 1:4

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  24. Content type: Research article

    The Gavac™ vaccine against the cattle tick Boophilus microplus has proven its efficacy in a large number of controlled and field experiments. However, this vaccine could be further improved by searching for new a...

    Authors: Manuel Rodríguez Valle, Carlos Montero, Héctor Machado and Marisdania Joglar

    Citation: BMC Biotechnology 2001 1:2

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  25. Content type: Methodology article

    Infections of bacterial cultures by bacteriophages are serious problems in biotechnological laboratories. Apart from such infections, prophage induction in the host cells may also be dangerous. Escherichia coli i...

    Authors: Agata Czyz, Marcin Los, Borys Wrobel and Grzegorz Wegrzyn

    Citation: BMC Biotechnology 2001 1:1

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