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  1. Enzymes have been extensively used in organic solvents to catalyze a variety of transformations of biological and industrial significance. It has been generally accepted that in dry aprotic organic solvents, e...

    Authors: Betzaida Castillo, Vibha Bansal, Ashok Ganesan, Peter Halling, Francesco Secundo, Amaris Ferrer, Kai Griebenow and Gabriel Barletta

    Citation: BMC Biotechnology 2006 6:51

    Content type: Research article

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  2. RNA interference (RNAi) was originally identified as a biological process in which short double-stranded RNA (dsRNA) suppress the expression of genes complimentary to the dsRNA. This cellular intrinsic gene si...

    Authors: Shunqing Wang, Zhenqi Shi, Wei Liu, Joel Jules and Xu Feng

    Citation: BMC Biotechnology 2006 6:50

    Content type: Methodology article

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  3. A variety of approaches to understanding protein structure and function require production of recombinant protein. Mammalian based expression systems have advantages over bacterial systems for certain classes ...

    Authors: Susan DJ Chapple, Anna M Crofts, S Paul Shadbolt, John McCafferty and Michael R Dyson

    Citation: BMC Biotechnology 2006 6:49

    Content type: Research article

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  4. The signal transducer and activator of transcription (STAT) family of transcription factors mediates a variety of cytokine dependent gene regulations. STAT1 has been mainly characterized by its role in interfe...

    Authors: Nicole R Leitner, Birgit Strobl, Marion Bokor, Ronald Painz, Thomas Kolbe, Thomas Rülicke, Mathias Müller and Marina Karaghiosoff

    Citation: BMC Biotechnology 2006 6:48

    Content type: Research article

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  5. Isolation of recombinant antibody fragments from antibody libraries is well established using technologies such as phage display. Phage display vectors are ideal for efficient display of antibody fragments on ...

    Authors: Cecile D Martin, Gertrudis Rojas, Joanne N Mitchell, Karen J Vincent, Jiahua Wu, John McCafferty and Darren J Schofield

    Citation: BMC Biotechnology 2006 6:46

    Content type: Research article

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  6. Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant t...

    Authors: Federica Bestoso, Laura Ottaggio, Andrea Armirotti, Alessandro Balbi, Gianluca Damonte, Paolo Degan, Mauro Mazzei, Francesca Cavalli, Bernardetta Ledda and Mariangela Miele

    Citation: BMC Biotechnology 2006 6:45

    Content type: Research article

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  7. In conventional PCR, total amplicon yield becomes independent of starting template number as amplification reaches plateau and varies significantly among replicate reactions. This paper describes a strategy fo...

    Authors: J Aquiles Sanchez, Jessica D Abramowitz, Jesse J Salk, Arthur H Reis Jr, John E Rice, Kenneth E Pierce and Lawrence J Wangh

    Citation: BMC Biotechnology 2006 6:44

    Content type: Methodology article

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  8. A number of expression systems have been developed where transgene expression can be regulated. They all have specific characteristics making them more suitable for certain applications than for others. Since ...

    Authors: Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie

    Citation: BMC Biotechnology 2006 6:43

    Content type: Methodology article

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  9. Tick-borne pathogens cause emerging zoonoses, and include fastidious organisms such as Anaplasma phagocytophilum. Because of their obligate intracellular nature, methods for mutagenesis and transformation have no...

    Authors: Roderick F Felsheim, Michael J Herron, Curtis M Nelson, Nicole Y Burkhardt, Anthony F Barbet, Timothy J Kurtti and Ulrike G Munderloh

    Citation: BMC Biotechnology 2006 6:42

    Content type: Methodology article

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  10. An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can be drawn. This can be done by using one or multiple, validated reference genes, depending...

    Authors: Tim Erkens, Mario Van Poucke, Jo Vandesompele, Karen Goossens, Alex Van Zeveren and Luc J Peelman

    Citation: BMC Biotechnology 2006 6:41

    Content type: Research article

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  11. Oncolytic herpes simplex virus (HSV) vectors that specifically replicate in and kill tumor cells sparing normal cells are a promising cancer therapy. Traditionally, recombinant HSV vectors have been generated ...

    Authors: Toshihiko Kuroda, Robert L Martuza, Tomoki Todo and Samuel D Rabkin

    Citation: BMC Biotechnology 2006 6:40

    Content type: Methodology article

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  12. The detection of Premature Stop Codons (PSCs) in human genes is very useful for the genetic diagnosis of different hereditary cancers, e.g. Familial Breast Cancer and Hereditary Non-Polyposis Colorectal Cancer...

    Authors: Sebastián M Real, Diego M Marzese, Laura C Gomez, Luis S Mayorga and María Roqué

    Citation: BMC Biotechnology 2006 6:38

    Content type: Methodology article

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  13. Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legisla...

    Authors: Katarina Cankar, Dejan Štebih, Tanja Dreo, Jana Žel and Kristina Gruden

    Citation: BMC Biotechnology 2006 6:37

    Content type: Methodology article

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  14. Oncolytic adenoviruses are promising agents for the multimodal treatment of cancer. However, tumor-selectivity is crucial for their applicability in patients. Recent studies by several groups demonstrated that...

    Authors: Dennis Hoffmann and Oliver Wildner

    Citation: BMC Biotechnology 2006 6:36

    Content type: Methodology article

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  15. Since a milestone work on Neisseria meningitidis B, Reverse Vaccinology has strongly enhanced the identification of vaccine candidates by replacing several experimental tasks using in silico prediction steps. The...

    Authors: Sandro Vivona, Filippo Bernante and Francesco Filippini

    Citation: BMC Biotechnology 2006 6:35

    Content type: Software

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  16. Lentiviral vectors are efficient vehicles for stable gene transfer in dividing and non-dividing cells. Several improvements in vector design to increase biosafety and transgene expression, have led to the appr...

    Authors: Martine Geraerts, Sofie Willems, Veerle Baekelandt, Zeger Debyser and Rik Gijsbers

    Citation: BMC Biotechnology 2006 6:34

    Content type: Research article

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  17. Insertional mutagenesis techniques with transposable elements have been popular among geneticists studying model organisms from E. coli to Drosophila and, more recently, the mouse. One such element is the Sleepin...

    Authors: Aron M Geurts, Andrew Wilber, Corey M Carlson, Paul D Lobitz, Karl J Clark, Perry B Hackett, R Scott McIvor and David A Largaespada

    Citation: BMC Biotechnology 2006 6:30

    Content type: Research article

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  18. Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus be...

    Authors: Gianpiero Marconi, Emidio Albertini, Pierluigi Barone, Francesca De Marchis, Chiara Lico, Carla Marusic, Domenico Rutili, Fabio Veronesi and Andrea Porceddu

    Citation: BMC Biotechnology 2006 6:29

    Content type: Research article

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  19. The use of small interfering RNAs (siRNAs) to silence target gene expression has greatly facilitated mammalian genetic analysis by generating loss-of-function mutants. In recent years, high-throughput, genome-...

    Authors: Cheng Du, Baosheng Ge, Zhongfeng Liu, Kai Fu, Wing C Chan and Timothy W McKeithan

    Citation: BMC Biotechnology 2006 6:28

    Content type: Methodology article

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  20. Germ-line transformation of insects is now a widely used method for analyzing gene function and for the development of genetically modified strains suitable for pest control programs. The most widely used tran...

    Authors: Abhimanyu Sarkar, Asela Atapattu, Esther J Belikoff, Jörg C Heinrich, Xuelei Li, Carsten Horn, Ernst A Wimmer and Maxwell J Scott

    Citation: BMC Biotechnology 2006 6:27

    Content type: Methodology article

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  21. The use of integrating viral vectors in Gene therapy clinical trials has pointed out the problem of the deleterous effect of the integration of the ectopic gene to the cellular genome and the safety of this st...

    Authors: Bettina Couderc, Marie Penary, Mustapha Tohfe, Anne Pradines, Antoine Casteignau, Danièle Berg and Gilles Favre

    Citation: BMC Biotechnology 2006 6:26

    Content type: Research article

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  22. TH1 immune response antagonism is a desirable approach to mitigate some autoimmune and inflammatory reactions during the course of several diseases where IL-2 and IFN-γ are two central players. Therefore, the ...

    Authors: Iraldo Bello-Rivero, Yeny Torrez-Ruiz, Elizabeth Blanco-Garcés, Giselle Pentón-Rol, Osmani Fernández-Batista, Luís Javier-González, Haydee Gerónimo-Perez and Pedro López-Saura

    Citation: BMC Biotechnology 2006 6:25

    Content type: Research article

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  23. Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps in comparing transcription profiles...

    Authors: Lies Bogaert, Mario Van Poucke, Cindy De Baere, Luc Peelman, Frank Gasthuys and Ann Martens

    Citation: BMC Biotechnology 2006 6:24

    Content type: Methodology article

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  24. Whole-cell biocatalysis in organic solvents has been widely applied to industrial bioprocesses. In two-phase water-solvent processes, substrate conversion yields and volumetric productivities can be limited by...

    Authors: Heung-Chae Jung, Seok-Joon Kwon and Jae-Gu Pan

    Citation: BMC Biotechnology 2006 6:23

    Content type: Research article

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  25. Dihydrofolate reductase (DHFR) and thymidylate synthase (TS) are crucial enzymes in DNA synthesis. In alveolata both enzymes are expressed as one bifunctional enzyme.

    Authors: Lutz Herrmann, Ulrike Bockau, Arno Tiedtke, Marcus WW Hartmann and Thomas Weide

    Citation: BMC Biotechnology 2006 6:21

    Content type: Research article

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  26. A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons...

    Authors: Robert Hasterok, Joanna Dulawa, Glyn Jenkins, Mike Leggett and Tim Langdon

    Citation: BMC Biotechnology 2006 6:20

    Content type: Methodology article

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  27. The non-pathogenic ciliate Tetrahymena thermophila is one of the best-characterized unicellular eucaryotes used in various research fields. Previous work has shown that this unicellular organism provides many bio...

    Authors: Thomas Weide, Lutz Herrmann, Ulrike Bockau, Nadine Niebur, Ingo Aldag, Wouter Laroy, Roland Contreras, Arno Tiedtke and Marcus WW Hartmann

    Citation: BMC Biotechnology 2006 6:19

    Content type: Research article

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  28. Despite evidence suggesting a role in plant defence, the use of plant lectins in crop protection has been hindered by their low and species-specific insecticidal activity. Snowdrop lectin (Galanthus nivalis agglu...

    Authors: Nghia Pham Trung, Elaine Fitches and John A Gatehouse

    Citation: BMC Biotechnology 2006 6:18

    Content type: Research article

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  29. Minicircle DNA is the non-replicating product of intramolecular site-specific recombination within a bacterial minicircle producer plasmid. Minicircle DNA can be engineered to contain predominantly human seque...

    Authors: Oleg Tolmachov, Iwona Palaszewski, Brian Bigger and Charles Coutelle

    Citation: BMC Biotechnology 2006 6:17

    Content type: Research article

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  30. Optical imaging is an attractive non-invasive way to evaluate the expression of a transferred DNA, mainly thanks to its lower cost and ease of realization. In this study optical imaging was evaluated for monit...

    Authors: C Bloquel, C Trollet, E Pradines, J Seguin, D Scherman and MF Bureau

    Citation: BMC Biotechnology 2006 6:16

    Content type: Research article

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  31. The identification of protein trafficking signals, and their interacting mechanisms, is a fundamental objective of modern biology. Unfortunately, the analysis of trafficking signals is complicated by their top...

    Authors: Lynn McKeown, Philip Robinson, Sam M Greenwood, Weiwen Hu and Owen T Jones

    Citation: BMC Biotechnology 2006 6:15

    Content type: Methodology article

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  32. The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of ...

    Authors: Kurt J Langenbach, John T Elliott, Alex Tona, Dennis McDaniel and Anne L Plant

    Citation: BMC Biotechnology 2006 6:14

    Content type: Methodology article

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  33. Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In th...

    Authors: Karen Colwill, Clark D Wells, Kelly Elder, Marilyn Goudreault, Kadija Hersi, Sarang Kulkarni, W Rod Hardy, Tony Pawson and Gregg B Morin

    Citation: BMC Biotechnology 2006 6:13

    Content type: Methodology article

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  34. In the past few years, both automated and manual high-throughput protein expression and purification has become an accessible means to rapidly screen and produce soluble proteins for structural and functional ...

    Authors: Lisa D Cabrita, Weiwen Dai and Stephen P Bottomley

    Citation: BMC Biotechnology 2006 6:12

    Content type: Methodology article

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  35. Adenovirus (Ad) mediated gene transfer is a well-established tool to transiently express constructs in livers of mice in vivo. In the present study, we determined the specificity and efficiency of Ad vectors expr...

    Authors: Yvonne D Krom, Frits J Fallaux, Ivo Que, Clemens Lowik and Ko Willems van Dijk

    Citation: BMC Biotechnology 2006 6:11

    Content type: Research article

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  36. The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (β2GPI) is thought to co...

    Authors: Yiannis Ioannou, Ian Giles, Anastasia Lambrianides, Chris Richardson, Laurence H Pearl, David S Latchman, David A Isenberg and Anisur Rahman

    Citation: BMC Biotechnology 2006 6:8

    Content type: Methodology article

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  37. RNA interference (RNAi) technology is a powerful methodology recently developed for the specific knockdown of targeted genes. RNAi is most commonly achieved either transiently by transfection of small interfer...

    Authors: Debra J Taxman, Laura R Livingstone, Jinghua Zhang, Brian J Conti, Heather A Iocca, Kristi L Williams, John D Lich, Jenny P-Y Ting and William Reed

    Citation: BMC Biotechnology 2006 6:7

    Content type: Methodology article

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  38. Haplosclerid marine sponges produce pore forming polyalkylpyridinium salts (poly-APS), which can be used to deliver macromolecules into cells. The aim of this study was to investigate the delivery of DNA, siRN...

    Authors: Debra McLaggan, Noppadon Adjimatera, Kristina Sepčić, Marcel Jaspars, David J MacEwan, Ian S Blagbrough and Roderick H Scott

    Citation: BMC Biotechnology 2006 6:6

    Content type: Research article

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  39. We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing bec...

    Authors: Daniel Gendron, Sandra Carriero, Daniel Garneau, Jonathan Villemaire, Roscoe Klinck, Sherif Abou Elela, Masad J Damha and Benoit Chabot

    Citation: BMC Biotechnology 2006 6:5

    Content type: Methodology article

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  40. Internal Ribosome Entry Site (IRES)-based bicistronic vectors are important tools in today's cell biology. Among applications, the expression of two proteins under the control of a unique promoter permits the ...

    Authors: Patrick Martin, Olivier Albagli, Marie Christine Poggi, Kim E Boulukos and Philippe Pognonec

    Citation: BMC Biotechnology 2006 6:4

    Content type: Methodology article

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