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Page 27 of 30

  1. Targeted mutagenesis of the herpesvirus genomes has been facilitated by the use of bacterial artificial chromosome (BAC) technology. Such modified genomes have potential uses in understanding viral pathogenesi...

    Authors: Falko Schmeisser and Jerry P Weir

    Citation: BMC Biotechnology 2007 7:22

    Content type: Methodology article

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  2. The ability to accurately measure patterns of gene expression is essential in studying gene function. The reverse transcription polymerase chain reaction (RT-PCR) has become the method of choice for the detect...

    Authors: Fadia Haddad, Anqi X Qin, Julie M Giger, Hongyan Guo and Kenneth M Baldwin

    Citation: BMC Biotechnology 2007 7:21

    Content type: Methodology article

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  3. Angiogenesis assays are important tools for the identification of regulatory molecules and the potential development of therapeutic strategies to modulate neovascularization. Although numerous in vitro angioge...

    Authors: Bastien Hermant, Agnès Desroches-Castan, Marie-Laure Dubessay, Marie-Hélène Prandini, Philippe Huber and Daniel Vittet

    Citation: BMC Biotechnology 2007 7:20

    Content type: Methodology article

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  4. In protein drug development, in vitro molecular optimization or protein maturation can be used to modify protein properties. One basic approach to protein maturation is the introduction of random DNA mutations in...

    Authors: George Kopsidas, Rachael K Carman, Emma L Stutt, Anna Raicevic, Anthony S Roberts, Mary-Anne V Siomos, Nada Dobric, Luisa Pontes-Braz and Greg Coia

    Citation: BMC Biotechnology 2007 7:18

    Content type: Methodology article

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  5. Embryonated chicken eggs have been used since the mid-20th century to grow a wide range of animal viruses to high titers. However, eggs have found so far only limited use in the production of recombinant prote...

    Authors: Teresa Corral, Lorena S Ver, Geneviève Mottet, Olga Cano, Blanca García-Barreno, Lesley J Calder, John J Skehel, Laurent Roux and José A Melero

    Citation: BMC Biotechnology 2007 7:17

    Content type: Methodology article

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  6. Altering a protein's function by changing its sequence allows natural proteins to be converted into useful molecular tools. Current protein engineering methods are limited by a lack of high throughput physical...

    Authors: Jun Liao, Manfred K Warmuth, Sridhar Govindarajan, Jon E Ness, Rebecca P Wang, Claes Gustafsson and Jeremy Minshull

    Citation: BMC Biotechnology 2007 7:16

    Content type: Research article

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  7. A goal for the food industry has always been to improve strains of Lactococcus lactis and stabilize beneficial traits. Genetic engineering is used extensively for manipulating this lactic acid bacterium, while el...

    Authors: Maria Papagianni, Nicholaos Avramidis and George Filioussis

    Citation: BMC Biotechnology 2007 7:15

    Content type: Methodology article

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  8. The connection of the variable part of the heavy chain (VH) and and the variable part of the light chain (VL) by a peptide linker to form a consecutive polypeptide chain (single chain antibody, scFv) was a bre...

    Authors: Michael Hust, Thomas Jostock, Christian Menzel, Bernd Voedisch, Anja Mohr, Mariam Brenneis, Martina I Kirsch, Doris Meier and Stefan Dübel

    Citation: BMC Biotechnology 2007 7:14

    Content type: Research article

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  9. Bacteriophage infections of bacterial cultures cause serious problems in genetic engineering and biotechnology. They are dangerous not only because of direct effects on the currently infected cultures, i.e. th...

    Authors: Marcin Łoś, Piotr Golec, Joanna M Łoś, Anna Węglewska-Jurkiewicz, Agata Czyż, Alicja Węgrzyn, Grzegorz Węgrzyn and Peter Neubauer

    Citation: BMC Biotechnology 2007 7:13

    Content type: Correspondence

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  10. Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increa...

    Authors: Carla Marusic, James Nuttall, Giampaolo Buriani, Chiara Lico, Raffaele Lombardi, Selene Baschieri, Eugenio Benvenuto and Lorenzo Frigerio

    Citation: BMC Biotechnology 2007 7:12

    Content type: Research article

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  11. Strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome which complicates the breeding of new cultivars. For certain traits, genetic engineering offers a potential alte...

    Authors: Kati J Hanhineva and Sirpa O Kärenlampi

    Citation: BMC Biotechnology 2007 7:11

    Content type: Methodology article

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  12. Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses...

    Authors: Saima Khanam, Pilankatta Rajendra, Navin Khanna and Sathyamangalam Swaminathan

    Citation: BMC Biotechnology 2007 7:10

    Content type: Research article

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  13. Despite several effective treatment options available for prostate cancer, it remains the second leading cause of cancer death in American men. Thus, there is a great need for new treatments to improve outcome...

    Authors: Jan Woraratanadharm, Semyon Rubinchik, Hong Yu and John Y Dong

    Citation: BMC Biotechnology 2007 7:9

    Content type: Research article

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  14. Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies. Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy...

    Authors: William S Branham, Cathy D Melvin, Tao Han, Varsha G Desai, Carrie L Moland, Adam T Scully and James C Fuscoe

    Citation: BMC Biotechnology 2007 7:8

    Content type: Methodology article

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  15. Recombinant antibodies from Camelidae (VHHs) are potentially useful tools for both basic research and biotechnological applications because of their small size, robustness, easy handling and possibility to ref...

    Authors: Aurelien Olichon, Daniel Schweizer, Serge Muyldermans and Ario de Marco

    Citation: BMC Biotechnology 2007 7:7

    Content type: Methodology article

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  16. The Tet-Off (tTA) and Tet-On (rtTA) regulatory systems are widely applied to control gene expression in eukaryotes. Both systems are based on the Tet repressor (TetR) from transposon Tn10, a dimeric DNA-binding p...

    Authors: Xue Zhou, Jori Symons, Rieuwert Hoppes, Christel Krueger, Christian Berens, Wolfgang Hillen, Ben Berkhout and Atze T Das

    Citation: BMC Biotechnology 2007 7:6

    Content type: Research article

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  17. We constructed and characterized several new piggyBac vectors to provide transposition of constitutively- or inducibly-expressible heterologous gene pairs. The dual constitutive control element consists of back-t...

    Authors: Xianzong Shi, Robert L Harrison, Jason R Hollister, Ahmed Mohammed, Malcolm J Fraser Jr and Donald L Jarvis

    Citation: BMC Biotechnology 2007 7:5

    Content type: Research article

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  18. Fluorescence activated cell sorting (FACS) is a powerful technique for the qualitative and quantitative detection of biomolecules used widely in both basic research and clinical diagnostic applications. Beads ...

    Authors: B Thomas Bäckström, Jane A Brockelbank and Bernd HA Rehm

    Citation: BMC Biotechnology 2007 7:3

    Content type: Research article

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  19. Advances in fluorescence microscopy and mouse transgenesis have made it possible to image molecular events in living animals. However, the generation of transgenic mice is a lengthy process and intravital imag...

    Authors: Adriaan D Bins, Jacco van Rheenen, Kees Jalink, Jonathan R Halstead, Nullin Divecha, David M Spencer, John BAG Haanen and Ton NM Schumacher

    Citation: BMC Biotechnology 2007 7:2

    Content type: Methodology article

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  20. The cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses the capacity to promote the effective uptake of various cargo molecules across the plasma membr...

    Authors: Johanna Rinne, Brian Albarran, Juulia Jylhävä, Teemu O Ihalainen, Pasi Kankaanpää, Vesa P Hytönen, Patrick S Stayton, Markku S Kulomaa and Maija Vihinen-Ranta

    Citation: BMC Biotechnology 2007 7:1

    Content type: Research article

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  21. The availability of cell lines releasing fluorescent viral particles can significantly support a variety of investigations, including the study of virus-cell interaction and the screening of antiviral compound...

    Authors: Claudia Muratori, Paola D'Aloja, Fabiana Superti, Antonella Tinari, Nathalie Sol-Foulon, Sandra Sparacio, Valerie Bosch, Olivier Schwartz and Maurizio Federico

    Citation: BMC Biotechnology 2006 6:52

    Content type: Research article

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  22. Enzymes have been extensively used in organic solvents to catalyze a variety of transformations of biological and industrial significance. It has been generally accepted that in dry aprotic organic solvents, e...

    Authors: Betzaida Castillo, Vibha Bansal, Ashok Ganesan, Peter Halling, Francesco Secundo, Amaris Ferrer, Kai Griebenow and Gabriel Barletta

    Citation: BMC Biotechnology 2006 6:51

    Content type: Research article

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  23. RNA interference (RNAi) was originally identified as a biological process in which short double-stranded RNA (dsRNA) suppress the expression of genes complimentary to the dsRNA. This cellular intrinsic gene si...

    Authors: Shunqing Wang, Zhenqi Shi, Wei Liu, Joel Jules and Xu Feng

    Citation: BMC Biotechnology 2006 6:50

    Content type: Methodology article

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  24. A variety of approaches to understanding protein structure and function require production of recombinant protein. Mammalian based expression systems have advantages over bacterial systems for certain classes ...

    Authors: Susan DJ Chapple, Anna M Crofts, S Paul Shadbolt, John McCafferty and Michael R Dyson

    Citation: BMC Biotechnology 2006 6:49

    Content type: Research article

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  25. The signal transducer and activator of transcription (STAT) family of transcription factors mediates a variety of cytokine dependent gene regulations. STAT1 has been mainly characterized by its role in interfe...

    Authors: Nicole R Leitner, Birgit Strobl, Marion Bokor, Ronald Painz, Thomas Kolbe, Thomas Rülicke, Mathias Müller and Marina Karaghiosoff

    Citation: BMC Biotechnology 2006 6:48

    Content type: Research article

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  26. Isolation of recombinant antibody fragments from antibody libraries is well established using technologies such as phage display. Phage display vectors are ideal for efficient display of antibody fragments on ...

    Authors: Cecile D Martin, Gertrudis Rojas, Joanne N Mitchell, Karen J Vincent, Jiahua Wu, John McCafferty and Darren J Schofield

    Citation: BMC Biotechnology 2006 6:46

    Content type: Research article

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  27. Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant t...

    Authors: Federica Bestoso, Laura Ottaggio, Andrea Armirotti, Alessandro Balbi, Gianluca Damonte, Paolo Degan, Mauro Mazzei, Francesca Cavalli, Bernardetta Ledda and Mariangela Miele

    Citation: BMC Biotechnology 2006 6:45

    Content type: Research article

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  28. In conventional PCR, total amplicon yield becomes independent of starting template number as amplification reaches plateau and varies significantly among replicate reactions. This paper describes a strategy fo...

    Authors: J Aquiles Sanchez, Jessica D Abramowitz, Jesse J Salk, Arthur H Reis Jr, John E Rice, Kenneth E Pierce and Lawrence J Wangh

    Citation: BMC Biotechnology 2006 6:44

    Content type: Methodology article

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  29. A number of expression systems have been developed where transgene expression can be regulated. They all have specific characteristics making them more suitable for certain applications than for others. Since ...

    Authors: Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie

    Citation: BMC Biotechnology 2006 6:43

    Content type: Methodology article

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  30. Tick-borne pathogens cause emerging zoonoses, and include fastidious organisms such as Anaplasma phagocytophilum. Because of their obligate intracellular nature, methods for mutagenesis and transformation have no...

    Authors: Roderick F Felsheim, Michael J Herron, Curtis M Nelson, Nicole Y Burkhardt, Anthony F Barbet, Timothy J Kurtti and Ulrike G Munderloh

    Citation: BMC Biotechnology 2006 6:42

    Content type: Methodology article

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  31. An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can be drawn. This can be done by using one or multiple, validated reference genes, depending...

    Authors: Tim Erkens, Mario Van Poucke, Jo Vandesompele, Karen Goossens, Alex Van Zeveren and Luc J Peelman

    Citation: BMC Biotechnology 2006 6:41

    Content type: Research article

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  32. Oncolytic herpes simplex virus (HSV) vectors that specifically replicate in and kill tumor cells sparing normal cells are a promising cancer therapy. Traditionally, recombinant HSV vectors have been generated ...

    Authors: Toshihiko Kuroda, Robert L Martuza, Tomoki Todo and Samuel D Rabkin

    Citation: BMC Biotechnology 2006 6:40

    Content type: Methodology article

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  33. The detection of Premature Stop Codons (PSCs) in human genes is very useful for the genetic diagnosis of different hereditary cancers, e.g. Familial Breast Cancer and Hereditary Non-Polyposis Colorectal Cancer...

    Authors: Sebastián M Real, Diego M Marzese, Laura C Gomez, Luis S Mayorga and María Roqué

    Citation: BMC Biotechnology 2006 6:38

    Content type: Methodology article

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  34. Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legisla...

    Authors: Katarina Cankar, Dejan Štebih, Tanja Dreo, Jana Žel and Kristina Gruden

    Citation: BMC Biotechnology 2006 6:37

    Content type: Methodology article

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  35. Oncolytic adenoviruses are promising agents for the multimodal treatment of cancer. However, tumor-selectivity is crucial for their applicability in patients. Recent studies by several groups demonstrated that...

    Authors: Dennis Hoffmann and Oliver Wildner

    Citation: BMC Biotechnology 2006 6:36

    Content type: Methodology article

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  36. Since a milestone work on Neisseria meningitidis B, Reverse Vaccinology has strongly enhanced the identification of vaccine candidates by replacing several experimental tasks using in silico prediction steps. The...

    Authors: Sandro Vivona, Filippo Bernante and Francesco Filippini

    Citation: BMC Biotechnology 2006 6:35

    Content type: Software

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  37. Lentiviral vectors are efficient vehicles for stable gene transfer in dividing and non-dividing cells. Several improvements in vector design to increase biosafety and transgene expression, have led to the appr...

    Authors: Martine Geraerts, Sofie Willems, Veerle Baekelandt, Zeger Debyser and Rik Gijsbers

    Citation: BMC Biotechnology 2006 6:34

    Content type: Research article

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  38. Insertional mutagenesis techniques with transposable elements have been popular among geneticists studying model organisms from E. coli to Drosophila and, more recently, the mouse. One such element is the Sleepin...

    Authors: Aron M Geurts, Andrew Wilber, Corey M Carlson, Paul D Lobitz, Karl J Clark, Perry B Hackett, R Scott McIvor and David A Largaespada

    Citation: BMC Biotechnology 2006 6:30

    Content type: Research article

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  39. Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus be...

    Authors: Gianpiero Marconi, Emidio Albertini, Pierluigi Barone, Francesca De Marchis, Chiara Lico, Carla Marusic, Domenico Rutili, Fabio Veronesi and Andrea Porceddu

    Citation: BMC Biotechnology 2006 6:29

    Content type: Research article

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  40. The use of small interfering RNAs (siRNAs) to silence target gene expression has greatly facilitated mammalian genetic analysis by generating loss-of-function mutants. In recent years, high-throughput, genome-...

    Authors: Cheng Du, Baosheng Ge, Zhongfeng Liu, Kai Fu, Wing C Chan and Timothy W McKeithan

    Citation: BMC Biotechnology 2006 6:28

    Content type: Methodology article

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  41. Germ-line transformation of insects is now a widely used method for analyzing gene function and for the development of genetically modified strains suitable for pest control programs. The most widely used tran...

    Authors: Abhimanyu Sarkar, Asela Atapattu, Esther J Belikoff, Jörg C Heinrich, Xuelei Li, Carsten Horn, Ernst A Wimmer and Maxwell J Scott

    Citation: BMC Biotechnology 2006 6:27

    Content type: Methodology article

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