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  1. An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can be drawn. This can be done by using one or multiple, validated reference genes, depending...

    Authors: Tim Erkens, Mario Van Poucke, Jo Vandesompele, Karen Goossens, Alex Van Zeveren and Luc J Peelman

    Citation: BMC Biotechnology 2006 6:41

    Content type: Research article

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  2. Oncolytic herpes simplex virus (HSV) vectors that specifically replicate in and kill tumor cells sparing normal cells are a promising cancer therapy. Traditionally, recombinant HSV vectors have been generated ...

    Authors: Toshihiko Kuroda, Robert L Martuza, Tomoki Todo and Samuel D Rabkin

    Citation: BMC Biotechnology 2006 6:40

    Content type: Methodology article

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  3. The detection of Premature Stop Codons (PSCs) in human genes is very useful for the genetic diagnosis of different hereditary cancers, e.g. Familial Breast Cancer and Hereditary Non-Polyposis Colorectal Cancer...

    Authors: Sebastián M Real, Diego M Marzese, Laura C Gomez, Luis S Mayorga and María Roqué

    Citation: BMC Biotechnology 2006 6:38

    Content type: Methodology article

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  4. Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legisla...

    Authors: Katarina Cankar, Dejan Štebih, Tanja Dreo, Jana Žel and Kristina Gruden

    Citation: BMC Biotechnology 2006 6:37

    Content type: Methodology article

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  5. Oncolytic adenoviruses are promising agents for the multimodal treatment of cancer. However, tumor-selectivity is crucial for their applicability in patients. Recent studies by several groups demonstrated that...

    Authors: Dennis Hoffmann and Oliver Wildner

    Citation: BMC Biotechnology 2006 6:36

    Content type: Methodology article

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  6. Since a milestone work on Neisseria meningitidis B, Reverse Vaccinology has strongly enhanced the identification of vaccine candidates by replacing several experimental tasks using in silico prediction steps. The...

    Authors: Sandro Vivona, Filippo Bernante and Francesco Filippini

    Citation: BMC Biotechnology 2006 6:35

    Content type: Software

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  7. Lentiviral vectors are efficient vehicles for stable gene transfer in dividing and non-dividing cells. Several improvements in vector design to increase biosafety and transgene expression, have led to the appr...

    Authors: Martine Geraerts, Sofie Willems, Veerle Baekelandt, Zeger Debyser and Rik Gijsbers

    Citation: BMC Biotechnology 2006 6:34

    Content type: Research article

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  8. Insertional mutagenesis techniques with transposable elements have been popular among geneticists studying model organisms from E. coli to Drosophila and, more recently, the mouse. One such element is the Sleepin...

    Authors: Aron M Geurts, Andrew Wilber, Corey M Carlson, Paul D Lobitz, Karl J Clark, Perry B Hackett, R Scott McIvor and David A Largaespada

    Citation: BMC Biotechnology 2006 6:30

    Content type: Research article

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  9. Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus be...

    Authors: Gianpiero Marconi, Emidio Albertini, Pierluigi Barone, Francesca De Marchis, Chiara Lico, Carla Marusic, Domenico Rutili, Fabio Veronesi and Andrea Porceddu

    Citation: BMC Biotechnology 2006 6:29

    Content type: Research article

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  10. The use of small interfering RNAs (siRNAs) to silence target gene expression has greatly facilitated mammalian genetic analysis by generating loss-of-function mutants. In recent years, high-throughput, genome-...

    Authors: Cheng Du, Baosheng Ge, Zhongfeng Liu, Kai Fu, Wing C Chan and Timothy W McKeithan

    Citation: BMC Biotechnology 2006 6:28

    Content type: Methodology article

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  11. Germ-line transformation of insects is now a widely used method for analyzing gene function and for the development of genetically modified strains suitable for pest control programs. The most widely used tran...

    Authors: Abhimanyu Sarkar, Asela Atapattu, Esther J Belikoff, Jörg C Heinrich, Xuelei Li, Carsten Horn, Ernst A Wimmer and Maxwell J Scott

    Citation: BMC Biotechnology 2006 6:27

    Content type: Methodology article

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  12. The use of integrating viral vectors in Gene therapy clinical trials has pointed out the problem of the deleterous effect of the integration of the ectopic gene to the cellular genome and the safety of this st...

    Authors: Bettina Couderc, Marie Penary, Mustapha Tohfe, Anne Pradines, Antoine Casteignau, Danièle Berg and Gilles Favre

    Citation: BMC Biotechnology 2006 6:26

    Content type: Research article

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  13. TH1 immune response antagonism is a desirable approach to mitigate some autoimmune and inflammatory reactions during the course of several diseases where IL-2 and IFN-γ are two central players. Therefore, the ...

    Authors: Iraldo Bello-Rivero, Yeny Torrez-Ruiz, Elizabeth Blanco-Garcés, Giselle Pentón-Rol, Osmani Fernández-Batista, Luís Javier-González, Haydee Gerónimo-Perez and Pedro López-Saura

    Citation: BMC Biotechnology 2006 6:25

    Content type: Research article

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  14. Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps in comparing transcription profiles...

    Authors: Lies Bogaert, Mario Van Poucke, Cindy De Baere, Luc Peelman, Frank Gasthuys and Ann Martens

    Citation: BMC Biotechnology 2006 6:24

    Content type: Methodology article

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  15. Whole-cell biocatalysis in organic solvents has been widely applied to industrial bioprocesses. In two-phase water-solvent processes, substrate conversion yields and volumetric productivities can be limited by...

    Authors: Heung-Chae Jung, Seok-Joon Kwon and Jae-Gu Pan

    Citation: BMC Biotechnology 2006 6:23

    Content type: Research article

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  16. Dihydrofolate reductase (DHFR) and thymidylate synthase (TS) are crucial enzymes in DNA synthesis. In alveolata both enzymes are expressed as one bifunctional enzyme.

    Authors: Lutz Herrmann, Ulrike Bockau, Arno Tiedtke, Marcus WW Hartmann and Thomas Weide

    Citation: BMC Biotechnology 2006 6:21

    Content type: Research article

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  17. A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons...

    Authors: Robert Hasterok, Joanna Dulawa, Glyn Jenkins, Mike Leggett and Tim Langdon

    Citation: BMC Biotechnology 2006 6:20

    Content type: Methodology article

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  18. The non-pathogenic ciliate Tetrahymena thermophila is one of the best-characterized unicellular eucaryotes used in various research fields. Previous work has shown that this unicellular organism provides many bio...

    Authors: Thomas Weide, Lutz Herrmann, Ulrike Bockau, Nadine Niebur, Ingo Aldag, Wouter Laroy, Roland Contreras, Arno Tiedtke and Marcus WW Hartmann

    Citation: BMC Biotechnology 2006 6:19

    Content type: Research article

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  19. Despite evidence suggesting a role in plant defence, the use of plant lectins in crop protection has been hindered by their low and species-specific insecticidal activity. Snowdrop lectin (Galanthus nivalis agglu...

    Authors: Nghia Pham Trung, Elaine Fitches and John A Gatehouse

    Citation: BMC Biotechnology 2006 6:18

    Content type: Research article

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  20. Minicircle DNA is the non-replicating product of intramolecular site-specific recombination within a bacterial minicircle producer plasmid. Minicircle DNA can be engineered to contain predominantly human seque...

    Authors: Oleg Tolmachov, Iwona Palaszewski, Brian Bigger and Charles Coutelle

    Citation: BMC Biotechnology 2006 6:17

    Content type: Research article

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  21. Optical imaging is an attractive non-invasive way to evaluate the expression of a transferred DNA, mainly thanks to its lower cost and ease of realization. In this study optical imaging was evaluated for monit...

    Authors: C Bloquel, C Trollet, E Pradines, J Seguin, D Scherman and MF Bureau

    Citation: BMC Biotechnology 2006 6:16

    Content type: Research article

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  22. The identification of protein trafficking signals, and their interacting mechanisms, is a fundamental objective of modern biology. Unfortunately, the analysis of trafficking signals is complicated by their top...

    Authors: Lynn McKeown, Philip Robinson, Sam M Greenwood, Weiwen Hu and Owen T Jones

    Citation: BMC Biotechnology 2006 6:15

    Content type: Methodology article

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  23. The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of ...

    Authors: Kurt J Langenbach, John T Elliott, Alex Tona, Dennis McDaniel and Anne L Plant

    Citation: BMC Biotechnology 2006 6:14

    Content type: Methodology article

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  24. Recombinational systems have been developed to rapidly shuttle Open Reading Frames (ORFs) into multiple expression vectors in order to analyze the large number of cDNAs available in the post-genomic era. In th...

    Authors: Karen Colwill, Clark D Wells, Kelly Elder, Marilyn Goudreault, Kadija Hersi, Sarang Kulkarni, W Rod Hardy, Tony Pawson and Gregg B Morin

    Citation: BMC Biotechnology 2006 6:13

    Content type: Methodology article

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  25. In the past few years, both automated and manual high-throughput protein expression and purification has become an accessible means to rapidly screen and produce soluble proteins for structural and functional ...

    Authors: Lisa D Cabrita, Weiwen Dai and Stephen P Bottomley

    Citation: BMC Biotechnology 2006 6:12

    Content type: Methodology article

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  26. Adenovirus (Ad) mediated gene transfer is a well-established tool to transiently express constructs in livers of mice in vivo. In the present study, we determined the specificity and efficiency of Ad vectors expr...

    Authors: Yvonne D Krom, Frits J Fallaux, Ivo Que, Clemens Lowik and Ko Willems van Dijk

    Citation: BMC Biotechnology 2006 6:11

    Content type: Research article

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  27. The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (β2GPI) is thought to co...

    Authors: Yiannis Ioannou, Ian Giles, Anastasia Lambrianides, Chris Richardson, Laurence H Pearl, David S Latchman, David A Isenberg and Anisur Rahman

    Citation: BMC Biotechnology 2006 6:8

    Content type: Methodology article

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  28. RNA interference (RNAi) technology is a powerful methodology recently developed for the specific knockdown of targeted genes. RNAi is most commonly achieved either transiently by transfection of small interfer...

    Authors: Debra J Taxman, Laura R Livingstone, Jinghua Zhang, Brian J Conti, Heather A Iocca, Kristi L Williams, John D Lich, Jenny P-Y Ting and William Reed

    Citation: BMC Biotechnology 2006 6:7

    Content type: Methodology article

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  29. Haplosclerid marine sponges produce pore forming polyalkylpyridinium salts (poly-APS), which can be used to deliver macromolecules into cells. The aim of this study was to investigate the delivery of DNA, siRN...

    Authors: Debra McLaggan, Noppadon Adjimatera, Kristina Sepčić, Marcel Jaspars, David J MacEwan, Ian S Blagbrough and Roderick H Scott

    Citation: BMC Biotechnology 2006 6:6

    Content type: Research article

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  30. We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing bec...

    Authors: Daniel Gendron, Sandra Carriero, Daniel Garneau, Jonathan Villemaire, Roscoe Klinck, Sherif Abou Elela, Masad J Damha and Benoit Chabot

    Citation: BMC Biotechnology 2006 6:5

    Content type: Methodology article

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  31. Internal Ribosome Entry Site (IRES)-based bicistronic vectors are important tools in today's cell biology. Among applications, the expression of two proteins under the control of a unique promoter permits the ...

    Authors: Patrick Martin, Olivier Albagli, Marie Christine Poggi, Kim E Boulukos and Philippe Pognonec

    Citation: BMC Biotechnology 2006 6:4

    Content type: Methodology article

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  32. The use of lactic acid bacteria as vehicles to delivery antigens to immunize animals is a promising issue. When genetically modified, these bacteria can induce a specific local and systemic immune response aga...

    Authors: Rodrigo M Mota, João Luiz S Moreira, Marcelo R Souza, M Fátima Horta, Santuza MR Teixeira, Elisabeth Neumann, Jacques R Nicoli and Álvaro C Nunes

    Citation: BMC Biotechnology 2006 6:2

    Content type: Research article

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  33. The isozymes of alkaline phosphatase, the tissue non-specific, intestinal and placental, have similar properties and a high degree of identity. The placental isozyme (PLAP) is an oncofetal antigen expressed in...

    Authors: Deepti Saini, Mrinalini Kala, Vishal Jain and Subrata Sinha

    Citation: BMC Biotechnology 2005 5:33

    Content type: Research article

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  34. Parthenocarpic tomato lines transgenic for the DefH9-RI-iaaM gene have been cultivated under open field conditions to address some aspects of the equivalence of genetically modified (GM) fruit in comparison to co...

    Authors: Giuseppe Leonardo Rotino, Nazareno Acciarri, Emidio Sabatini, Giuseppe Mennella, Roberto Lo Scalzo, Andrea Maestrelli, Barbara Molesini, Tiziana Pandolfini, Jessica Scalzo, Bruno Mezzetti and Angelo Spena

    Citation: BMC Biotechnology 2005 5:32

    Content type: Research article

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  35. As real-time quantitative PCR (RT-QPCR) is increasingly being relied upon for the enforcement of legislation and regulations dependent upon the trace detection of DNA, focus has increased on the quality issues...

    Authors: Malcolm J Burns, Gavin J Nixon, Carole A Foy and Neil Harris

    Citation: BMC Biotechnology 2005 5:31

    Content type: Research article

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  36. Efficient targeting to appropriate cell organelles is one of the bottlenecks for the production of recombinant proteins in plant systems. A common practice is to use the native secretory signal peptide of the ...

    Authors: Andreas Schaaf, Stefanie Tintelnot, Armin Baur, Ralf Reski, Gilbert Gorr and Eva L Decker

    Citation: BMC Biotechnology 2005 5:30

    Content type: Research article

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  37. The chicken avidin gene family consists of avidin and several avidin related genes (AVRs). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a pro...

    Authors: Vesa P Hytönen, Juha AE Määttä, Heidi Kidron, Katrin K Halling, Jarno Hörhä, Tuomas Kulomaa, Thomas KM Nyholm, Mark S Johnson, Tiina A Salminen, Markku S Kulomaa and Tomi T Airenne

    Citation: BMC Biotechnology 2005 5:28

    Content type: Research article

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  38. Precise analysis of expression-regulating elements, such as enhancers and insulators, requires that they be tested under reproducible, isogenic conditions. The commonly used methods of transfecting DNA into ce...

    Authors: Emily Y Cheng, Cathy Collins, Maribel Berru and Marc J Shulman

    Citation: BMC Biotechnology 2005 5:27

    Content type: Methodology article

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  39. The definite diagnosis of prion diseases such as Creutzfeldt-Jakob disease (CJD) in humans or bovine spongiform encephalopathy (BSE) in cattle currently relies on the post mortem detection of the pathological for...

    Authors: Lothar Trieschmann, Alexander Navarrete Santos, Katja Kaschig, Sandra Torkler, Elke Maas, Hermann Schätzl and Gerald Böhm

    Citation: BMC Biotechnology 2005 5:26

    Content type: Research article

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