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  1. Cryopreservation of preantral follicles or ovarian tissues would enable the storage of large numbers of primordial follicles or preantral follicles and preserves the structural integrity of somatic and reprodu...

    Authors: Ta-Chin Lin, Jui-Mei Yen, Tsung-Cheng Kuo, Kun-Bing Gong, Kung-Hao Hsu and Teng-Tsao Hsu

    Citation: BMC Biotechnology 2008 8:38

    Content type: Methodology article

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  2. Patatins encoded by a multi-gene family are one of the major storage glycoproteins in potato tubers. Potato tubers have recently emerged as bioreactors for the production of human therapeutic glycoproteins (va...

    Authors: Yoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung and Jae-Heung Jeon

    Citation: BMC Biotechnology 2008 8:36

    Content type: Research article

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  3. Exon skipping oligonucleotides (ESOs) of 2'O-Methyl (2'OMe) and morpholino chemistry have been shown to restore dystrophin expression in muscle fibers from the mdx mouse, and are currently being tested in phase I...

    Authors: Jason H Williams, Rebecca C Schray, Shashank R Sirsi and Gordon J Lutz

    Citation: BMC Biotechnology 2008 8:35

    Content type: Research article

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  4. The Rho GTPases A, B and C proteins, members of the Rho family whose activity is regulated by GDP/GTP cycling, function in many cellular pathways controlling proliferation and have recently been implicated in ...

    Authors: Marine Goffinet, Patrick Chinestra, Isabelle Lajoie-Mazenc, Claire Medale-Giamarchi, Gilles Favre and Jean-Charles Faye

    Citation: BMC Biotechnology 2008 8:34

    Content type: Research article

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  5. Here we describe a new technical solution for optimization of Pichia pastoris shake flask cultures with the example of production of stable human type II collagen. Production of recombinant proteins in P. pastori...

    Authors: Maria Ruottinen, Monika Bollok, Martin Kögler, Antje Neubauer, Mirja Krause, Eija-Riitta Hämäläinen, Johanna Myllyharju, Antti Vasala and Peter Neubauer

    Citation: BMC Biotechnology 2008 8:33

    Content type: Methodology article

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  6. The last decade has brought the renaissance of protein studies and accelerated the development of high-throughput methods in all aspects of proteomics. Presently, most protein synthesis systems exploit the cap...

    Authors: Viola Bardóczy, Viktória Géczi, Tatsuya Sawasaki, Yaeta Endo and Tamás Mészáros

    Citation: BMC Biotechnology 2008 8:32

    Content type: Methodology article

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  7. Gene targeting in nonhuman primates has the potential to produce critical animal models for translational studies related to human diseases. Successful gene targeting in fibroblasts followed by somatic cell nu...

    Authors: Daniel T Meehan, Mary Ann Zink, Melissa Mahlen, Marilu Nelson, Warren G Sanger, Shoukhrat M Mitalipov, Don P Wolf, Michel M Ouellette and Robert B Norgren Jr

    Citation: BMC Biotechnology 2008 8:31

    Content type: Methodology article

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  8. β-Lactams like penicillin and cephalosporin are among the oldest known antibiotics used against bacterial infections. Industrially, penicillin is produced by the filamentous fungus Penicillium chrysogenum. Our go...

    Authors: Loknath Gidijala, Roel AL Bovenberg, Paul Klaassen, Ida J van der Klei, Marten Veenhuis and Jan AKW Kiel

    Citation: BMC Biotechnology 2008 8:29

    Content type: Research article

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  9. Soybeans play a prominent role in allergologic research due to the high incidence of allergic reactions. For detailed studies on specific proteins it is necessary to have access to a large amount of pure subst...

    Authors: Eva Sewekow, Lars Christian Kessler, Andreas Seidel-Morgenstern and Hermann-Josef Rothkötter

    Citation: BMC Biotechnology 2008 8:27

    Content type: Methodology article

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  10. The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying ...

    Authors: Meti Buh Gašparič, Katarina Cankar, Jana Žel and Kristina Gruden

    Citation: BMC Biotechnology 2008 8:26

    Content type: Methodology article

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  11. In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduce...

    Authors: Anna Aksamit-Stachurska, Alina Korobczak-Sosna, Anna Kulma and Jan Szopa

    Citation: BMC Biotechnology 2008 8:25

    Content type: Research article

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  12. Over the last decade, kinases have emerged as attractive therapeutic targets for a number of different diseases, and numerous high throughput screening efforts in the pharmaceutical community are directed towa...

    Authors: Lihao Meng, Gregory A Michaud, Janie S Merkel, Fang Zhou, Jing Huang, Dawn R Mattoon and Barry Schweitzer

    Citation: BMC Biotechnology 2008 8:22

    Content type: Methodology article

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  13. Zebrafish are increasingly used to study the influences of gene mutation and manipulation on cardiac development, structure and function. In this study, a video edge detection system was used to characterise, ...

    Authors: Martin A Denvir, Carl S Tucker and John J Mullins

    Citation: BMC Biotechnology 2008 8:21

    Content type: Research article

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  14. Serum and high ionic strength solutions constitute important barriers to cationic lipid-mediated intravenous gene transfer. Preparation or incubation of lipoplexes in these media results in alteration of their...

    Authors: Catarina Madeira, Luís MS Loura, Manuel Prieto, Aleksander Fedorov and M Raquel Aires-Barros

    Citation: BMC Biotechnology 2008 8:20

    Content type: Research article

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  15. Retroviruses are widely used to transfer genes to mammalian cells efficiently and stably. However, genetic elements required for high-level gene expression are incompatible with standard systems. The retrovira...

    Authors: Magnus Blø, David R Micklem and James B Lorens

    Citation: BMC Biotechnology 2008 8:19

    Content type: Methodology article

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  16. Inducible conditional knockout animals are widely used to get insight in the function of genes and the pathogenesis of human diseases. These models frequently rely on Cre-mediated recombination of sequences fl...

    Authors: Wouter N Leonhard, Jeroen H Roelfsema, Irma S Lantinga-van Leeuwen, Martijn H Breuning and Dorien JM Peters

    Citation: BMC Biotechnology 2008 8:18

    Content type: Methodology article

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  17. Whole genome amplification (WGA) and laser assisted micro-dissection represent two recently developed technologies that can greatly advance biological and medical research. WGA allows the analysis of multiple ...

    Authors: Dan Frumkin, Adam Wasserstrom, Shalev Itzkovitz, Alon Harmelin, Gideon Rechavi and Ehud Shapiro

    Citation: BMC Biotechnology 2008 8:17

    Content type: Methodology article

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  18. The recognition that human tumors stimulate the production of autoantibodies has initiated the use of this immune response as serological markers for the early diagnosis and management of cancer. The enzyme-li...

    Authors: Ratchada Cressey, Saranya Pimpa, Busyamas Chewaskulyong, Nirush Lertprasertsuke, Somchareon Saeteng, Chatchai Tayapiwatana and Watchara Kasinrerk

    Citation: BMC Biotechnology 2008 8:16

    Content type: Research article

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  19. The most efficient method for enhancing solubility of recombinant proteins appears to use the fusion expression partners. Although commercial fusion partners including maltose binding protein and glutathione-S-tr...

    Authors: Jin-Seung Park, Kyung-Yeon Han, Jong-Ho Lee, Jong-Am Song, Keum-Young Ahn, Hyuk-Seong Seo, Sang-Jun Sim, Seung-Wook Kim and Jeewon Lee

    Citation: BMC Biotechnology 2008 8:15

    Content type: Research article

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  20. Rhipicephalus (Boophilus) spp. ticks economically impact on cattle production in Africa and other tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally fr...

    Authors: Mario Canales, José M Pérez de la Lastra, Victoria Naranjo, Ard M Nijhof, Michelle Hope, Frans Jongejan and José de la Fuente

    Citation: BMC Biotechnology 2008 8:14

    Content type: Research article

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  21. Human cell lines are the most innovative choice of host cell for production of biopharmaceuticals since they allow for authentic posttranslational modification of therapeutic proteins. We present a new method ...

    Authors: Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Waschütza and Christoph Volpers

    Citation: BMC Biotechnology 2008 8:13

    Content type: Research article

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  22. Combination of CHD (chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-inten...

    Authors: Hsueh-Wei Chang, Chun-An Cheng, De-Leung Gu, Chia-Che Chang, San-Hua Su, Cheng-Hao Wen, Yii-Cheng Chou, Ta-Ching Chou, Cheng-Te Yao, Chi-Li Tsai and Chien-Chung Cheng

    Citation: BMC Biotechnology 2008 8:12

    Content type: Methodology article

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  23. Elevations of serum prolactin (PRL) are associated with an increased risk for breast cancer. PRL signaling through its prolactin receptor (PRLr) involves the Jak2/Stat5 pathway. Luciferase-based reporter assay...

    Authors: Feng Fang, Giovanni Antico, Jiamao Zheng and Charles V Clevenger

    Citation: BMC Biotechnology 2008 8:11

    Content type: Methodology article

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  24. Archival formalin-fixed paraffin-embedded (FFPE) tissues represent an abundant source of clinical specimens; however their use is limited in applications involving analysis of gene expression due to RNA degrad...

    Authors: Jinghuan Li, Paul Smyth, Susanne Cahill, Karen Denning, Richard Flavin, Sinead Aherne, Marco Pirotta, Simone M Guenther, John J O'Leary and Orla Sheils

    Citation: BMC Biotechnology 2008 8:10

    Content type: Research article

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  25. Previous studies indicated that recombinant gas vesicles (r-GV) from a mutant strain of Halobacterium sp. NRC-1 could express a cassette containing test sequences of SIVmac gag derived DNA, and function as an ant...

    Authors: Marinko Sremac and Elizabeth S Stuart

    Citation: BMC Biotechnology 2008 8:9

    Content type: Research article

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  26. Enzymes have found extensive and growing application in the field of chemical organic synthesis and resolution of chiral intermediates. In order to stabilise the enzymes and to facilitate their recovery and re...

    Authors: Dean Brady, Justin Jordaan, Clinton Simpson, Avashnee Chetty, Cherise Arumugam and Francis S Moolman

    Citation: BMC Biotechnology 2008 8:8

    Content type: Research article

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  27. Femtosecond (fs) laser pulses have recently received wide interest as an alternative tool for manipulating living biological systems. In various model organisms the excision of cellular components and the intr...

    Authors: Vikram Kohli and Abdulhakem Y Elezzabi

    Citation: BMC Biotechnology 2008 8:7

    Content type: Research article

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  28. High purity of tumour samples is a necessity for accurate genetic and expression analysis and is usually achieved by positive selection in chronic lymphocytic leukaemia (CLL).

    Authors: Salim Essakali, Dennis Carney, David Westerman, Peter Gambell, John F Seymour and Alexander Dobrovic

    Citation: BMC Biotechnology 2008 8:6

    Content type: Methodology article

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  29. Expression of intracellular antibodies (intrabodies) has become a broadly applicable technology for generation of phenotypic knockouts in vivo. The method uses surface depletion of cellular membrane proteins to e...

    Authors: Khajornsak Tragoolpua, Nutjeera Intasai, Watchara Kasinrerk, Sabine Mai, Yuan Yuan and Chatchai Tayapiwatana

    Citation: BMC Biotechnology 2008 8:5

    Content type: Research article

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  30. For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneou...

    Authors: Zhengbing Jiang, Bei Gao, Ren Ren, Xingyi Tao, Yushu Ma and Dongzhi Wei

    Citation: BMC Biotechnology 2008 8:4

    Content type: Research article

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  31. The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such,...

    Authors: Natalie Gavrilov-Yusim, Ekaterina Hahiashvili, Marina Tashker, Victoria Yavelsky, Ohad Karnieli and Leslie Lobel

    Citation: BMC Biotechnology 2008 8:3

    Content type: Research article

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  32. A capable expression vector is mainly characterized by its production efficiency, stability and induction response. These features can be influenced by a variation of modifications and versatile genetic modules.

    Authors: Angelika Wegerer, Tianqi Sun and Josef Altenbuchner

    Citation: BMC Biotechnology 2008 8:2

    Content type: Research article

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  33. Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis.

    Authors: Ricardo Pinto, António L Amaral, Eugénio C Ferreira, Manuel Mota, Manuel Vilanova, Katia Ruel and Miguel Gama

    Citation: BMC Biotechnology 2008 8:1

    Content type: Research article

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  34. HERG potassium channel blockade is the major cause for drug-induced long QT syndrome, which sometimes cause cardiac disrhythmias and sudden death. There is a strong interest in the pharmaceutical industry to d...

    Authors: Mao Xiang Chen, Shaun L Sandow, Virginie Doceul, Yu Hua Chen, Heather Harper, Bruce Hamilton, Helen J Meadows, Derek J Trezise and Jeff J Clare

    Citation: BMC Biotechnology 2007 7:93

    Content type: Research article

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  35. GFP-fusion proteins and immunostaining are methods broadly applied to investigate the three-dimensional organization of cells and cell nuclei, the latter often studied in addition by fluorescence in situ hybri...

    Authors: Il-Han Kim, Jens Nagel, Simone Otten, Boris Knerr, Roland Eils, Karl Rohr and Steffen Dietzel

    Citation: BMC Biotechnology 2007 7:92

    Content type: Research article

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  36. Due to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed...

    Authors: Torstein Tengs, Anja B Kristoffersen, Knut G Berdal, Tage Thorstensen, Melinka A Butenko, Håvard Nesvold and Arne Holst-Jensen

    Citation: BMC Biotechnology 2007 7:91

    Content type: Methodology article

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  37. Bone marrow-derived stromal cells (MSC) are attractive targets for ex vivo cell and gene therapy. In this context, we investigated the feasibility of a plasmid-based strategy for genetic modification of human (h)...

    Authors: Mark W Ronsyn, Jasmijn Daans, Gie Spaepen, Shyama Chatterjee, Katrien Vermeulen, Patrick D'Haese, Viggo FI Van Tendeloo, Eric Van Marck, Dirk Ysebaert, Zwi N Berneman, Philippe G Jorens and Peter Ponsaerts

    Citation: BMC Biotechnology 2007 7:90

    Content type: Research article

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  38. The acute sensitivity of some hybridoma cell lines to culture-related stresses severely limits their productivity. Recent developments in the characterization of the stress signals modulating the cellular phen...

    Authors: Matthew Mallory, Kevin Chartrand and Eric R Gauthier

    Citation: BMC Biotechnology 2007 7:89

    Content type: Research article

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  39. Conventional cell culture studies have been performed on 2D surfaces, resulting in flat, extended cell growth. More relevant studies are desired to better mimic 3D in vivo tissue growth. Such realistic environmen...

    Authors: Thomas Liebmann, Susanna Rydholm, Victor Akpe and Hjalmar Brismar

    Citation: BMC Biotechnology 2007 7:88

    Content type: Research article

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  40. The formation of two distinctive cell lineages in preimplantation mouse embryos is characterized by differential gene expression. The cells of the inner cell mass are pluripotent and express high levels of Oct4 m...

    Authors: Cristina Hartshorn, Judith J Eckert, Odelya Hartung and Lawrence J Wangh

    Citation: BMC Biotechnology 2007 7:87

    Content type: Methodology article

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  41. Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. Often, it is used in immobilised form. With conventional immobilisation t...

    Authors: Barry J Ryan and Ciarán Ó'Fágáin

    Citation: BMC Biotechnology 2007 7:86

    Content type: Research article

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  42. Retroviral vectors are valuable tools for gene transfer. Particularly convenient are IRES-containing retroviral vectors expressing both the protein of interest and a marker protein from a single bicistronic mR...

    Authors: Olivier Albagli-Curiel, Yann Lécluse, Philippe Pognonec, Kim E Boulukos and Patrick Martin

    Citation: BMC Biotechnology 2007 7:85

    Content type: Methodology article

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  43. Antibody-dependent cellular cytotoxicity (ADCC) is greatly enhanced by the absence of the core fucose of oligosaccharides attached to the Fc, and is closely related to the clinical efficacy of anticancer activ...

    Authors: Harue Imai-Nishiya, Katsuhiro Mori, Miho Inoue, Masako Wakitani, Shigeru Iida, Kenya Shitara and Mitsuo Satoh

    Citation: BMC Biotechnology 2007 7:84

    Content type: Research article

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  44. Virus-mediated delivery of therapeutic transgenes to the inflamed colon holds a great potential to serve as an effective therapeutic strategy for inflammatory bowel disease, since local, long-term expression o...

    Authors: Samuel J Farlow, Alan Jerusalmi and Takeshi Sano

    Citation: BMC Biotechnology 2007 7:83

    Content type: Research article

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