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  1. Cell-free protein synthesis is not only a rapid and high throughput technology to obtain proteins from their genes, but also provides an in vitro platform to study protein translation and folding. A detailed comp...

    Authors: Jason R Hillebrecht and Shaorong Chong

    Citation: BMC Biotechnology 2008 8:58

    Content type: Research article

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  2. Human papilloma virus (HPV) load and physical status are considered useful parameters for clinical evaluation of cervical squamous cell neoplasia. However, the errors implicit in HPV gene quantification by PCR...

    Authors: Ian Roberts, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff and Nicholas Coleman

    Citation: BMC Biotechnology 2008 8:57

    Content type: Research article

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  3. Although extensive research has been performed to control differentiation of neural stem cells – still, the response of those cells to diverse cell culture conditions often appears to be random and difficult t...

    Authors: Monika Witusik, Sylwester Piaskowski, Krystyna Hulas-Bigoszewska, Magdalena Zakrzewska, Sylwia M Gresner, S Ausim Azizi, Barbara Krynska, Pawel P Liberski and Piotr Rieske

    Citation: BMC Biotechnology 2008 8:56

    Content type: Research article

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  4. Regulated protein-protein interactions (PPIs) are pivotal molecular switches that are important for the regulation of signaling processes within eukaryotic cells. Cellular signaling is altered in various disea...

    Authors: Michael C Wehr, Lisa Reinecke, Anna Botvinnik and Moritz J Rossner

    Citation: BMC Biotechnology 2008 8:55

    Content type: Methodology article

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  5. The meniscus is a complex tissue whose cell biology has only recently begun to be explored. Published models rely upon initial culture in the presence of added growth factors. The aim of this study was to test...

    Authors: Helen E Gruber, David Mauerhan, Yin Chow, Jane A Ingram, H James Norton, Edward N Hanley Jr and Yubo Sun

    Citation: BMC Biotechnology 2008 8:54

    Content type: Research article

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  6. Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the developm...

    Authors: Ann Meyers, Ereck Chakauya, Enid Shephard, Fiona L Tanzer, James Maclean, Alisson Lynch, Anna-Lise Williamson and Edward P Rybicki

    Citation: BMC Biotechnology 2008 8:53

    Content type: Research article

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  7. Establishing a suitable level of exogenous gene expression in mammalian cells in general, and embryonic stem (ES) cells in particular, is an important aspect of understanding pathways of cell differentiation, ...

    Authors: Leonardo D'Aiuto, Clinton S Robison, Margherita Gigante, Edward Nwanegbo, Benjamin Shaffer, Meena Sukhwani, Carlos A Castro and J Richard Chaillet

    Citation: BMC Biotechnology 2008 8:52

    Content type: Methodology article

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  8. One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for ...

    Authors: Huajun Qin, Jian Hu, Yuanzhi Hua, Shridhar V Challa, Timothy A Cross and Fei P Gao

    Citation: BMC Biotechnology 2008 8:51

    Content type: Methodology article

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  9. Human butyrylcholinesterase (huBChE) has been shown to be an effective antidote against multiple LD50 of organophosphorus compounds. A prerequisite for such use of huBChE is a prolonged circulatory half-life. Thi...

    Authors: Yue-Jin Huang, Paul M Lundy, Anthoula Lazaris, Yue Huang, Hernan Baldassarre, Bin Wang, Carl Turcotte, Mélanie Côté, Annie Bellemare, Annie S Bilodeau, Sandra Brouillard, Madjid Touati, Peter Herskovits, Isabelle Bégin, Nathalie Neveu, Eric Brochu…

    Citation: BMC Biotechnology 2008 8:50

    Content type: Research article

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  10. Using cell-type-specific promoters to restrict gene expression to particular cells is an attractive approach for gene therapy, but often hampered by insufficient transcriptional activity of these promoters. Pr...

    Authors: Beihui Liu, Julian F Paton and Sergey Kasparov

    Citation: BMC Biotechnology 2008 8:49

    Content type: Research article

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  11. The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications. R...

    Authors: Thomas Naiser, Oliver Ehler, Jona Kayser, Timo Mai, Wolfgang Michel and Albrecht Ott

    Citation: BMC Biotechnology 2008 8:48

    Content type: Research article

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  12. Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification profile position. As such, absolute quantification requires construct...

    Authors: Robert G Rutledge and Don Stewart

    Citation: BMC Biotechnology 2008 8:47

    Content type: Methodology article

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  13. To ensure maximal productivity of recombinant proteins (rP) during production culture it is typical to encourage an initial phase of rapid cell proliferation to achieve high biomass followed by a stationary ph...

    Authors: Niraj Kumar, Patrick Gammell, Paula Meleady, Michael Henry and Martin Clynes

    Citation: BMC Biotechnology 2008 8:42

    Content type: Research article

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  14. Due to its extremely high strength, the interaction between biotin and (strept)avidin has been exploited for a large number of biotechnological applications. Site-specific biotinylation of proteins in vivo can be...

    Authors: Andrea Predonzani, Francesca Arnoldi, Alejandro López-Requena and Oscar R Burrone

    Citation: BMC Biotechnology 2008 8:41

    Content type: Research article

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  15. Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simu...

    Authors: Takako Noguchi, Masaaki Ikeda, Yoshihiro Ohmiya and Yoshihiro Nakajima

    Citation: BMC Biotechnology 2008 8:40

    Content type: Methodology article

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  16. Although many nerve prostheses have been proposed in recent years, in the case of consistent loss of nervous tissue peripheral nerve injury is still a traumatic pathology that may impair patient's movements by...

    Authors: Silvia Panseri, Carla Cunha, Joseph Lowery, Ubaldo Del Carro, Francesca Taraballi, Stefano Amadio, Angelo Vescovi and Fabrizio Gelain

    Citation: BMC Biotechnology 2008 8:39

    Content type: Research article

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  17. Cryopreservation of preantral follicles or ovarian tissues would enable the storage of large numbers of primordial follicles or preantral follicles and preserves the structural integrity of somatic and reprodu...

    Authors: Ta-Chin Lin, Jui-Mei Yen, Tsung-Cheng Kuo, Kun-Bing Gong, Kung-Hao Hsu and Teng-Tsao Hsu

    Citation: BMC Biotechnology 2008 8:38

    Content type: Methodology article

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  18. Patatins encoded by a multi-gene family are one of the major storage glycoproteins in potato tubers. Potato tubers have recently emerged as bioreactors for the production of human therapeutic glycoproteins (va...

    Authors: Yoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung and Jae-Heung Jeon

    Citation: BMC Biotechnology 2008 8:36

    Content type: Research article

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  19. Exon skipping oligonucleotides (ESOs) of 2'O-Methyl (2'OMe) and morpholino chemistry have been shown to restore dystrophin expression in muscle fibers from the mdx mouse, and are currently being tested in phase I...

    Authors: Jason H Williams, Rebecca C Schray, Shashank R Sirsi and Gordon J Lutz

    Citation: BMC Biotechnology 2008 8:35

    Content type: Research article

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  20. The Rho GTPases A, B and C proteins, members of the Rho family whose activity is regulated by GDP/GTP cycling, function in many cellular pathways controlling proliferation and have recently been implicated in ...

    Authors: Marine Goffinet, Patrick Chinestra, Isabelle Lajoie-Mazenc, Claire Medale-Giamarchi, Gilles Favre and Jean-Charles Faye

    Citation: BMC Biotechnology 2008 8:34

    Content type: Research article

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  21. Here we describe a new technical solution for optimization of Pichia pastoris shake flask cultures with the example of production of stable human type II collagen. Production of recombinant proteins in P. pastori...

    Authors: Maria Ruottinen, Monika Bollok, Martin Kögler, Antje Neubauer, Mirja Krause, Eija-Riitta Hämäläinen, Johanna Myllyharju, Antti Vasala and Peter Neubauer

    Citation: BMC Biotechnology 2008 8:33

    Content type: Methodology article

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  22. The last decade has brought the renaissance of protein studies and accelerated the development of high-throughput methods in all aspects of proteomics. Presently, most protein synthesis systems exploit the cap...

    Authors: Viola Bardóczy, Viktória Géczi, Tatsuya Sawasaki, Yaeta Endo and Tamás Mészáros

    Citation: BMC Biotechnology 2008 8:32

    Content type: Methodology article

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  23. Gene targeting in nonhuman primates has the potential to produce critical animal models for translational studies related to human diseases. Successful gene targeting in fibroblasts followed by somatic cell nu...

    Authors: Daniel T Meehan, Mary Ann Zink, Melissa Mahlen, Marilu Nelson, Warren G Sanger, Shoukhrat M Mitalipov, Don P Wolf, Michel M Ouellette and Robert B Norgren Jr

    Citation: BMC Biotechnology 2008 8:31

    Content type: Methodology article

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  24. β-Lactams like penicillin and cephalosporin are among the oldest known antibiotics used against bacterial infections. Industrially, penicillin is produced by the filamentous fungus Penicillium chrysogenum. Our go...

    Authors: Loknath Gidijala, Roel AL Bovenberg, Paul Klaassen, Ida J van der Klei, Marten Veenhuis and Jan AKW Kiel

    Citation: BMC Biotechnology 2008 8:29

    Content type: Research article

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  25. Soybeans play a prominent role in allergologic research due to the high incidence of allergic reactions. For detailed studies on specific proteins it is necessary to have access to a large amount of pure subst...

    Authors: Eva Sewekow, Lars Christian Kessler, Andreas Seidel-Morgenstern and Hermann-Josef Rothkötter

    Citation: BMC Biotechnology 2008 8:27

    Content type: Methodology article

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  26. The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying ...

    Authors: Meti Buh Gašparič, Katarina Cankar, Jana Žel and Kristina Gruden

    Citation: BMC Biotechnology 2008 8:26

    Content type: Methodology article

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  27. In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduce...

    Authors: Anna Aksamit-Stachurska, Alina Korobczak-Sosna, Anna Kulma and Jan Szopa

    Citation: BMC Biotechnology 2008 8:25

    Content type: Research article

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  28. Over the last decade, kinases have emerged as attractive therapeutic targets for a number of different diseases, and numerous high throughput screening efforts in the pharmaceutical community are directed towa...

    Authors: Lihao Meng, Gregory A Michaud, Janie S Merkel, Fang Zhou, Jing Huang, Dawn R Mattoon and Barry Schweitzer

    Citation: BMC Biotechnology 2008 8:22

    Content type: Methodology article

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  29. Zebrafish are increasingly used to study the influences of gene mutation and manipulation on cardiac development, structure and function. In this study, a video edge detection system was used to characterise, ...

    Authors: Martin A Denvir, Carl S Tucker and John J Mullins

    Citation: BMC Biotechnology 2008 8:21

    Content type: Research article

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  30. Serum and high ionic strength solutions constitute important barriers to cationic lipid-mediated intravenous gene transfer. Preparation or incubation of lipoplexes in these media results in alteration of their...

    Authors: Catarina Madeira, Luís MS Loura, Manuel Prieto, Aleksander Fedorov and M Raquel Aires-Barros

    Citation: BMC Biotechnology 2008 8:20

    Content type: Research article

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  31. Retroviruses are widely used to transfer genes to mammalian cells efficiently and stably. However, genetic elements required for high-level gene expression are incompatible with standard systems. The retrovira...

    Authors: Magnus Blø, David R Micklem and James B Lorens

    Citation: BMC Biotechnology 2008 8:19

    Content type: Methodology article

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  32. Inducible conditional knockout animals are widely used to get insight in the function of genes and the pathogenesis of human diseases. These models frequently rely on Cre-mediated recombination of sequences fl...

    Authors: Wouter N Leonhard, Jeroen H Roelfsema, Irma S Lantinga-van Leeuwen, Martijn H Breuning and Dorien JM Peters

    Citation: BMC Biotechnology 2008 8:18

    Content type: Methodology article

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  33. Whole genome amplification (WGA) and laser assisted micro-dissection represent two recently developed technologies that can greatly advance biological and medical research. WGA allows the analysis of multiple ...

    Authors: Dan Frumkin, Adam Wasserstrom, Shalev Itzkovitz, Alon Harmelin, Gideon Rechavi and Ehud Shapiro

    Citation: BMC Biotechnology 2008 8:17

    Content type: Methodology article

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  34. The recognition that human tumors stimulate the production of autoantibodies has initiated the use of this immune response as serological markers for the early diagnosis and management of cancer. The enzyme-li...

    Authors: Ratchada Cressey, Saranya Pimpa, Busyamas Chewaskulyong, Nirush Lertprasertsuke, Somchareon Saeteng, Chatchai Tayapiwatana and Watchara Kasinrerk

    Citation: BMC Biotechnology 2008 8:16

    Content type: Research article

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  35. The most efficient method for enhancing solubility of recombinant proteins appears to use the fusion expression partners. Although commercial fusion partners including maltose binding protein and glutathione-S-tr...

    Authors: Jin-Seung Park, Kyung-Yeon Han, Jong-Ho Lee, Jong-Am Song, Keum-Young Ahn, Hyuk-Seong Seo, Sang-Jun Sim, Seung-Wook Kim and Jeewon Lee

    Citation: BMC Biotechnology 2008 8:15

    Content type: Research article

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  36. Rhipicephalus (Boophilus) spp. ticks economically impact on cattle production in Africa and other tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally fr...

    Authors: Mario Canales, José M Pérez de la Lastra, Victoria Naranjo, Ard M Nijhof, Michelle Hope, Frans Jongejan and José de la Fuente

    Citation: BMC Biotechnology 2008 8:14

    Content type: Research article

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  37. Human cell lines are the most innovative choice of host cell for production of biopharmaceuticals since they allow for authentic posttranslational modification of therapeutic proteins. We present a new method ...

    Authors: Gudrun Schiedner, Sabine Hertel, Corinna Bialek, Helmut Kewes, Gero Waschütza and Christoph Volpers

    Citation: BMC Biotechnology 2008 8:13

    Content type: Research article

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  38. Combination of CHD (chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-inten...

    Authors: Hsueh-Wei Chang, Chun-An Cheng, De-Leung Gu, Chia-Che Chang, San-Hua Su, Cheng-Hao Wen, Yii-Cheng Chou, Ta-Ching Chou, Cheng-Te Yao, Chi-Li Tsai and Chien-Chung Cheng

    Citation: BMC Biotechnology 2008 8:12

    Content type: Methodology article

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  39. Elevations of serum prolactin (PRL) are associated with an increased risk for breast cancer. PRL signaling through its prolactin receptor (PRLr) involves the Jak2/Stat5 pathway. Luciferase-based reporter assay...

    Authors: Feng Fang, Giovanni Antico, Jiamao Zheng and Charles V Clevenger

    Citation: BMC Biotechnology 2008 8:11

    Content type: Methodology article

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  40. Archival formalin-fixed paraffin-embedded (FFPE) tissues represent an abundant source of clinical specimens; however their use is limited in applications involving analysis of gene expression due to RNA degrad...

    Authors: Jinghuan Li, Paul Smyth, Susanne Cahill, Karen Denning, Richard Flavin, Sinead Aherne, Marco Pirotta, Simone M Guenther, John J O'Leary and Orla Sheils

    Citation: BMC Biotechnology 2008 8:10

    Content type: Research article

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  41. Enzymes have found extensive and growing application in the field of chemical organic synthesis and resolution of chiral intermediates. In order to stabilise the enzymes and to facilitate their recovery and re...

    Authors: Dean Brady, Justin Jordaan, Clinton Simpson, Avashnee Chetty, Cherise Arumugam and Francis S Moolman

    Citation: BMC Biotechnology 2008 8:8

    Content type: Research article

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