Skip to main content

Advertisement

Articles

Page 24 of 31

  1. Employing genomic DNA clones to characterise gene attributes has several advantages over the use of cDNA clones, including the presence of native transcription and translation regulatory sequences as well as a...

    Authors: Kevin Rozwadowski, Wen Yang and Sateesh Kagale

    Citation: BMC Biotechnology 2008 8:88

    Content type: Methodology article

    Published on:

  2. Human glutamic acid decarboxylase 65 (hGAD65) is a key autoantigen in type 1 diabetes, having much potential as an important marker for the prediction and diagnosis of type 1 diabetes, and for the development ...

    Authors: Xiaofeng Wang, Martin Brandsma, Reynald Tremblay, Denis Maxwell, Anthony M Jevnikar, Norm Huner and Shengwu Ma

    Citation: BMC Biotechnology 2008 8:87

    Content type: Research article

    Published on:

  3. Array-based comparative genomic hybridization (CGH) and gene expression profiling have become vital techniques for identifying molecular defects underlying genetic diseases. Regardless of the microarray platfo...

    Authors: Mubasher Dar, Theresa Giesler, Rob Richardson, Christine Cai, Mike Cooper, Shahin Lavasani, Peter Kille, Thierry Voet and Joris Vermeesch

    Citation: BMC Biotechnology 2008 8:86

    Content type: Methodology article

    Published on:

  4. Human monoclonal antibodies (mAbs) generated as a result of the immune response are likely to be the most effective therapeutic antibodies, particularly in the case of infectious diseases against which the imm...

    Authors: Ada Funaro, Giorgio Gribaudo, Anna Luganini, Erika Ortolan, Nicola Lo Buono, Elisa Vicenzi, Luca Cassetta, Santo Landolfo, Richard Buick, Luca Falciola, Marianne Murphy, Gianni Garotta and Fabio Malavasi

    Citation: BMC Biotechnology 2008 8:85

    Content type: Research article

    Published on:

  5. Due to the global occurrence of multi-drug-resistant malarial parasites (Plasmodium falciparum), the anti-malarial drug most effective against malaria is artemisinin, a natural product (sesquiterpene lactone endo...

    Authors: Dae-Kyun Ro, Mario Ouellet, Eric M Paradise, Helcio Burd, Diana Eng, Chris J Paddon, Jack D Newman and Jay D Keasling

    Citation: BMC Biotechnology 2008 8:83

    Content type: Research article

    Published on:

  6. Oligomeric and fibrillar aggregates of the amyloid β-peptide (Aβ) have been implicated in the pathogenesis of Alzheimer's disease (AD). The characterization of Aβ assemblies is essential for the elucidation of...

    Authors: Bertil Macao, Wolfgang Hoyer, Anders Sandberg, Ann-Christin Brorsson, Christopher M Dobson and Torleif Härd

    Citation: BMC Biotechnology 2008 8:82

    Content type: Methodology article

    Published on:

  7. The dual-luciferase assay has been widely used in cell lines to determine rapidly but accurately the activity of a given promoter. Although this strategy has proved very useful, it does not allow the promoter ...

    Authors: Francisca Alcaraz-Pérez, Victoriano Mulero and María L Cayuela

    Citation: BMC Biotechnology 2008 8:81

    Content type: Methodology article

    Published on:

  8. Pseudomonas fluorescens is an important food spoilage organism, usually found in the form of biofilms. Bacterial biofilms are inherently resistant to a variety of antimicrobial agents, therefore alternative metho...

    Authors: Sanna Sillankorva, Peter Neubauer and Joana Azeredo

    Citation: BMC Biotechnology 2008 8:79

    Content type: Research article

    Published on:

  9. Several approaches can be used to functionalize biomaterials, such as hydrogels, for biomedical applications. One of the molecules often used to improve cells adhesion is the peptide Arg-Gly-Asp (RGD). The RGD...

    Authors: Susana M Moreira, Fábia K Andrade, Lucíla Domingues and Miguel Gama

    Citation: BMC Biotechnology 2008 8:78

    Content type: Research article

    Published on:

  10. Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation rea...

    Authors: Maartje MC Bastings, Ingrid van Baal, EW Meijer and Maarten Merkx

    Citation: BMC Biotechnology 2008 8:76

    Content type: Methodology article

    Published on:

  11. Apolipoprotein E (ApoE) is a molecular scavenger in the blood and brain. Aberrant function of the molecule causes formation of protein and lipid deposits or "plaques" that characterize Alzheimer's disease (AD)...

    Authors: Suzanne Zeitouni, Brian S Ford, Sean M Harris, Mandolin J Whitney, Carl A Gregory and Darwin J Prockop

    Citation: BMC Biotechnology 2008 8:75

    Content type: Research article

    Published on:

  12. Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth in...

    Authors: Xiu-Mei Cai, Hai-Long Xie, Ming-Zhu Liu and Xi-Liang Zha

    Citation: BMC Biotechnology 2008 8:74

    Content type: Methodology article

    Published on:

  13. The eukaryotic green alga, Chlamydomonas reinhardtii, produces H2 under anaerobic conditions, in a reaction catalysed by a [Fe-Fe] hydrogenase HydA1. For further biochemical and biophysical studies a suitable exp...

    Authors: Kateryna Sybirna, Tatiana Antoine, Pia Lindberg, Vincent Fourmond, Marc Rousset, Vincent Méjean and Hervé Bottin

    Citation: BMC Biotechnology 2008 8:73

    Content type: Research article

    Published on:

  14. Articular cartilage is exposed to high mechanical loads under normal physiological conditions and articular chondrocytes regulate the composition of cartilaginous matrix, in response to mechanical signals. How...

    Authors: Carole Bougault, Anne Paumier, Elisabeth Aubert-Foucher and Frédéric Mallein-Gerin

    Citation: BMC Biotechnology 2008 8:71

    Content type: Methodology article

    Published on:

  15. The aim of this experimental study on New Zealand's white rabbits was to find differences in the results of treating the distal physeal femoral defect by the transplantation of autologous or allogeneic mesench...

    Authors: Ladislav Planka, Petr Gal, Helga Kecova, Jiri Klima, Jana Hlucilova, Eva Filova, Evzen Amler, Petr Krupa, Leos Kren, Robert Srnec, Lucie Urbanova, Jana Lorenzova and Alois Necas

    Citation: BMC Biotechnology 2008 8:70

    Content type: Research article

    Published on:

  16. Determining the expression levels of microRNAs (miRNAs) is of great interest to researchers in many areas of biology, given the significant roles these molecules play in cellular regulation. Two common methods...

    Authors: Robert A Ach, Hui Wang and Bo Curry

    Citation: BMC Biotechnology 2008 8:69

    Content type: Research article

    Published on:

  17. The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable...

    Authors: Alessandra Mallano, Silvia Zamboni, Giulia Carpinelli, Filippo Santoro, Michela Flego, Alessandro Ascione, Mara Gellini, Marina Tombesi, Franca Podo and Maurizio Cianfriglia

    Citation: BMC Biotechnology 2008 8:68

    Content type: Research article

    Published on:

  18. Phosphoribosyl pyrophosphate (PRPP) is a central compound for cellular metabolism and may be considered as a link between carbon and nitrogen metabolism. PRPP is directly involved in the de novo and salvage biosy...

    Authors: Alberto Jiménez, María A Santos and José L Revuelta

    Citation: BMC Biotechnology 2008 8:67

    Content type: Research article

    Published on:

  19. Venezuelan equine encephalitis virus (VEEV) belongs to the Alphavirus group. Several species of this family are also pathogenic to humans and are recognized as potential agents of biological warfare and terror...

    Authors: Martina Inga Kirsch, Birgit Hülseweh, Christoph Nacke, Torsten Rülker, Thomas Schirrmann, Hans-Jürgen Marschall, Michael Hust and Stefan Dübel

    Citation: BMC Biotechnology 2008 8:66

    Content type: Research article

    Published on:

  20. Mammalian cells are becoming the prevailing expression system for the production of recombinant proteins because of their capacity for proper protein folding, assembly, and post-translational modifications. Th...

    Authors: Martin Loignon, Sylvie Perret, John Kelly, Denise Boulais, Brian Cass, Louis Bisson, Fatemeh Afkhamizarreh and Yves Durocher

    Citation: BMC Biotechnology 2008 8:65

    Content type: Research article

    Published on:

  21. Maize is a good model system for cereal crop genetics and development because of its rich genetic heritage and well-characterized morphology. The sequencing of its genome is well advanced, and new technologies...

    Authors: Helene Y Meller Harel, Veronique Fontaine, Hongying Chen, Ian M Jones and Paul A Millner

    Citation: BMC Biotechnology 2008 8:64

    Content type: Methodology article

    Published on:

  22. The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under...

    Authors: Natalia I Minaeva, Evgeny R Gak, Danila V Zimenkov, Aleksandra Yu Skorokhodova, Irina V Biryukova and Sergey V Mashko

    Citation: BMC Biotechnology 2008 8:63

    Content type: Methodology article

    Published on:

  23. Beta-thalassemia is a common autosomal recessive hereditary disease in the Meditertanean, Asia and African areas. Over 600 mutations have been described in the beta-globin (HBB), of which more than 200 are associ...

    Authors: Chia-Cheng Hung, Yi-Ning Su, Chia-Yun Lin, Yin-Fei Chang, Chien-Hui Chang, Wen-Fang Cheng, Chi-An Chen, Chien-Nan Lee and Win-Li Lin

    Citation: BMC Biotechnology 2008 8:62

    Content type: Methodology article

    Published on:

  24. Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending supp...

    Authors: Brian Moldt, Nicklas H Staunstrup, Maria Jakobsen, Rafael J Yáñez-Muñoz and Jacob G Mikkelsen

    Citation: BMC Biotechnology 2008 8:60

    Content type: Research article

    Published on:

  25. Bacteria use N-acyl homoserine lactone (AHL) molecules to regulate the expression of genes in a density-dependent manner. Several biosensors have been developed and engineered to detect the presence of all types ...

    Authors: Steve P Bernier, Anne L Beeston and Pamela A Sokol

    Citation: BMC Biotechnology 2008 8:59

    Content type: Methodology article

    Published on:

  26. Cell-free protein synthesis is not only a rapid and high throughput technology to obtain proteins from their genes, but also provides an in vitro platform to study protein translation and folding. A detailed comp...

    Authors: Jason R Hillebrecht and Shaorong Chong

    Citation: BMC Biotechnology 2008 8:58

    Content type: Research article

    Published on:

  27. Human papilloma virus (HPV) load and physical status are considered useful parameters for clinical evaluation of cervical squamous cell neoplasia. However, the errors implicit in HPV gene quantification by PCR...

    Authors: Ian Roberts, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff and Nicholas Coleman

    Citation: BMC Biotechnology 2008 8:57

    Content type: Research article

    Published on:

  28. Although extensive research has been performed to control differentiation of neural stem cells – still, the response of those cells to diverse cell culture conditions often appears to be random and difficult t...

    Authors: Monika Witusik, Sylwester Piaskowski, Krystyna Hulas-Bigoszewska, Magdalena Zakrzewska, Sylwia M Gresner, S Ausim Azizi, Barbara Krynska, Pawel P Liberski and Piotr Rieske

    Citation: BMC Biotechnology 2008 8:56

    Content type: Research article

    Published on:

  29. Regulated protein-protein interactions (PPIs) are pivotal molecular switches that are important for the regulation of signaling processes within eukaryotic cells. Cellular signaling is altered in various disea...

    Authors: Michael C Wehr, Lisa Reinecke, Anna Botvinnik and Moritz J Rossner

    Citation: BMC Biotechnology 2008 8:55

    Content type: Methodology article

    Published on:

  30. The meniscus is a complex tissue whose cell biology has only recently begun to be explored. Published models rely upon initial culture in the presence of added growth factors. The aim of this study was to test...

    Authors: Helen E Gruber, David Mauerhan, Yin Chow, Jane A Ingram, H James Norton, Edward N Hanley Jr and Yubo Sun

    Citation: BMC Biotechnology 2008 8:54

    Content type: Research article

    Published on:

  31. Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the developm...

    Authors: Ann Meyers, Ereck Chakauya, Enid Shephard, Fiona L Tanzer, James Maclean, Alisson Lynch, Anna-Lise Williamson and Edward P Rybicki

    Citation: BMC Biotechnology 2008 8:53

    Content type: Research article

    Published on:

  32. Establishing a suitable level of exogenous gene expression in mammalian cells in general, and embryonic stem (ES) cells in particular, is an important aspect of understanding pathways of cell differentiation, ...

    Authors: Leonardo D'Aiuto, Clinton S Robison, Margherita Gigante, Edward Nwanegbo, Benjamin Shaffer, Meena Sukhwani, Carlos A Castro and J Richard Chaillet

    Citation: BMC Biotechnology 2008 8:52

    Content type: Methodology article

    Published on:

  33. One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for ...

    Authors: Huajun Qin, Jian Hu, Yuanzhi Hua, Shridhar V Challa, Timothy A Cross and Fei P Gao

    Citation: BMC Biotechnology 2008 8:51

    Content type: Methodology article

    Published on:

  34. Human butyrylcholinesterase (huBChE) has been shown to be an effective antidote against multiple LD50 of organophosphorus compounds. A prerequisite for such use of huBChE is a prolonged circulatory half-life. Thi...

    Authors: Yue-Jin Huang, Paul M Lundy, Anthoula Lazaris, Yue Huang, Hernan Baldassarre, Bin Wang, Carl Turcotte, Mélanie Côté, Annie Bellemare, Annie S Bilodeau, Sandra Brouillard, Madjid Touati, Peter Herskovits, Isabelle Bégin, Nathalie Neveu, Eric Brochu…

    Citation: BMC Biotechnology 2008 8:50

    Content type: Research article

    Published on:

  35. Using cell-type-specific promoters to restrict gene expression to particular cells is an attractive approach for gene therapy, but often hampered by insufficient transcriptional activity of these promoters. Pr...

    Authors: Beihui Liu, Julian F Paton and Sergey Kasparov

    Citation: BMC Biotechnology 2008 8:49

    Content type: Research article

    Published on:

  36. The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications. R...

    Authors: Thomas Naiser, Oliver Ehler, Jona Kayser, Timo Mai, Wolfgang Michel and Albrecht Ott

    Citation: BMC Biotechnology 2008 8:48

    Content type: Research article

    Published on:

  37. Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification profile position. As such, absolute quantification requires construct...

    Authors: Robert G Rutledge and Don Stewart

    Citation: BMC Biotechnology 2008 8:47

    Content type: Methodology article

    Published on:

  38. To ensure maximal productivity of recombinant proteins (rP) during production culture it is typical to encourage an initial phase of rapid cell proliferation to achieve high biomass followed by a stationary ph...

    Authors: Niraj Kumar, Patrick Gammell, Paula Meleady, Michael Henry and Martin Clynes

    Citation: BMC Biotechnology 2008 8:42

    Content type: Research article

    Published on:

  39. Due to its extremely high strength, the interaction between biotin and (strept)avidin has been exploited for a large number of biotechnological applications. Site-specific biotinylation of proteins in vivo can be...

    Authors: Andrea Predonzani, Francesca Arnoldi, Alejandro López-Requena and Oscar R Burrone

    Citation: BMC Biotechnology 2008 8:41

    Content type: Research article

    Published on:

  40. Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simu...

    Authors: Takako Noguchi, Masaaki Ikeda, Yoshihiro Ohmiya and Yoshihiro Nakajima

    Citation: BMC Biotechnology 2008 8:40

    Content type: Methodology article

    Published on:

  41. Although many nerve prostheses have been proposed in recent years, in the case of consistent loss of nervous tissue peripheral nerve injury is still a traumatic pathology that may impair patient's movements by...

    Authors: Silvia Panseri, Carla Cunha, Joseph Lowery, Ubaldo Del Carro, Francesca Taraballi, Stefano Amadio, Angelo Vescovi and Fabrizio Gelain

    Citation: BMC Biotechnology 2008 8:39

    Content type: Research article

    Published on: