Skip to main content


RNAi mechanisms and applications

Section edited by John Rossi

This section will consider studies that focus on RNAi research in animal cells and their applications, including gene therapy.

  1. Content type: Research article

    Nonsense mutations promote premature translational termination, introducing stop codons within the coding region of mRNAs and causing inherited diseases, including thalassemia. For instance, in β039 thalassemia t...

    Authors: Francesca Salvatori, Mariangela Pappadà, Giulia Breveglieri, Elisabetta D’Aversa, Alessia Finotti, Ilaria Lampronti, Roberto Gambari and Monica Borgatti

    Citation: BMC Biotechnology 2018 18:28

    Published on:

  2. Content type: Methodology article

    Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian...

    Authors: Sander B. Frank, Veronique V. Schulz and Cindy K. Miranti

    Citation: BMC Biotechnology 2017 17:24

    Published on:

  3. Content type: Research article

    RNAi (RNA interference) is a technology for silencing of target genes via sequence-specific manner. RNAi technology has been used for development of anti-pathogenic crops. In 2007, development of transgenic plant...

    Authors: Oyunchuluun Ganbaatar, Budao Cao, Yanan Zhang, Duran Bao, Wenhua Bao and Hada Wuriyanghan

    Citation: BMC Biotechnology 2017 17:9

    Published on:

  4. Content type: Research article

    RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five treh...

    Authors: Bin Tang, Ping Wei, Lina Zhao, Zuokun Shi, Qida Shen, Mengmeng Yang, Guoqiang Xie and Shigui Wang

    Citation: BMC Biotechnology 2016 16:67

    Published on:

  5. Content type: Methodology article

    RNA interference (RNAi) is an important molecular tool for analysis of gene function in vivo. Daphnia, a freshwater microcrustacean, is an emerging model organism for studying cellular and molecular processes inv...

    Authors: Charles A. Schumpert, Jeffry L. Dudycha and Rekha C. Patel

    Citation: BMC Biotechnology 2015 15:91

    Published on:

  6. Content type: Methodology article

    Human pancreatic islet structure poses challenges to investigations that require specific modulation of gene expression. Yet dissociation of islets into individual cells destroys cellular interactions importan...

    Authors: Stanley Tamaki, Christopher Nye, Euan Slorach, David Scharp, Helen M Blau, Phyllis E Whiteley and Jason H Pomerantz

    Citation: BMC Biotechnology 2014 14:86

    Published on:

  7. Content type: Methodology article

    The dose-response relationship is a fundamental pharmacological parameter necessary to determine therapeutic thresholds. Epi-allelic hypomorphic analysis using RNA interference (RNAi) can similarly correlate t...

    Authors: David R Micklem, Magnus Blø, Petra Bergström, Erlend Hodneland, Crina Tiron, Torill Høiby, Christine Gjerdrum, Ola Hammarsten and James B Lorens

    Citation: BMC Biotechnology 2014 14:57

    Published on:

  8. Content type: Research article

    Human adenoviruses are a frequent threat to immunocompromised patients, and disseminated disease is associated with severe morbidity and mortality. Current drugs are not capable of preventing all fatalities, t...

    Authors: Mirza Ibrišimović, Thomas Lion and Reinhard Klein

    Citation: BMC Biotechnology 2013 13:54

    Published on:

  9. Content type: Research article

    Controlling and limiting the expression of short hairpin RNA (shRNA) by using constitutive or tissue-specific polymerase II (pol II) expression can be a promising strategy to avoid RNAi toxicity. However, to d...

    Authors: Piotr Maczuga, Annemart Koornneef, Florie Borel, Harald Petry, Sander van Deventer, Tita Ritsema and Pavlina Konstantinova

    Citation: BMC Biotechnology 2012 12:42

    Published on: