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Protein and enzyme technology

This section will consider studies that focus on recombinant proteins and enzymes, cell-free protein expression systems, and biocatalysis using immobilized enzymes.

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  1. BioH is one of the key enzymes to produce the precursor pimeloyl-ACP to initiate biotin biosynthesis de novo in bacteria. To date, very few bioH genes have been characterized. In this study, we cloned and identif...

    Authors: Yuping Shi, Yingjie Pan, Bailin Li, Wei He, Qunxin She and Lanming Chen

    Citation: BMC Biotechnology 2013 13:13

    Content type: Research article

    Published on:

  2. Expression and purification of correctly folded proteins typically require screening of different parameters such as protein variants, solubility enhancing tags or expression hosts. Parallel vector series that...

    Authors: Judith Scholz, Hüseyin Besir, Claudia Strasser and Sabine Suppmann

    Citation: BMC Biotechnology 2013 13:12

    Content type: Methodology article

    Published on:

  3. From a human health viewpoint, contaminated milk and its products could be a source of long-term exposure to toxic metals. Simple, inexpensive, and on-site assays would enable constant monitoring of their cont...

    Authors: Mohammad Shohel Rana Siddiki, Shunsaku Ueda and Isamu Maeda

    Citation: BMC Biotechnology 2012 12:76

    Content type: Methodology article

    Published on:

  4. There are a growing number of reports on the sub-physiological temperature culturing of mammalian cells for increased recombinant protein yields. However, the effect varies and the reasons for the enhancement ...

    Authors: Yasuhiko Sumitomo, Hiroaki Higashitsuji, Hisako Higashitsuji, Yu Liu, Takanori Fujita, Toshiharu Sakurai, Marco M Candeias, Katsuhiko Itoh, Tsutomu Chiba and Jun Fujita

    Citation: BMC Biotechnology 2012 12:72

    Content type: Research article

    Published on:

  5. Protein glycosylation is of fundamental importance in many biological systems. The discovery of N-glycosylation in bacteria and the functional expression of the N-oligosaccharyltransferase PglB of Campylobacter j...

    Authors: Julian Ihssen, Michael Kowarik, Luzia Wiesli, Renate Reiss, Michael Wacker and Linda Thöny-Meyer

    Citation: BMC Biotechnology 2012 12:67

    Content type: Research article

    Published on:

  6. Conventional fluorescent proteins, such as GFP, its derivatives and flavin mononucleotide based fluorescent proteins (FbFPs) are often used as fusion tags for detecting recombinant proteins during cultivation....

    Authors: Eva-Maria Siepert, Esther Gartz, Mehmet Kemal Tur, Heinrich Delbrück, Stefan Barth and Jochen Büchs

    Citation: BMC Biotechnology 2012 12:65

    Content type: Research article

    Published on:

  7. Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and pu...

    Authors: Jason S Buhrman, Jamie E Rayahin, Melanie Köllmer and Richard A Gemeinhart

    Citation: BMC Biotechnology 2012 12:63

    Content type: Methodology article

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  8. Microbial lipases particularly Pseudomonas lipases are widely used for biotechnological applications. It is a meaningful work to design experiments to obtain high-level active lipase. There is a limiting factor f...

    Authors: Xiangping Wu, Pengyong You, Erzheng Su, Jingjing Xu, Bei Gao and Dongzhi Wei

    Citation: BMC Biotechnology 2012 12:58

    Content type: Research article

    Published on:

  9. Covalent linkage of the ubiquitin-like protein ISG15 interferes with viral infection and USP18 is the major protease which specifically removes ISG15 from target proteins. Thus, boosting ISG15 modification by ...

    Authors: Anja Basters, Lars Ketscher, Elke Deuerling, Christoph Arkona, Jörg Rademann, Klaus-Peter Knobeloch and Günter Fritz

    Citation: BMC Biotechnology 2012 12:56

    Content type: Methodology article

    Published on:

  10. Cost-effective production of industrially important enzymes is a key for their successful exploitation on industrial scale. Keeping in view the extensive industrial applications of lignin peroxidase (LiP), thi...

    Authors: Muhammad Asgher, Hafiz Muhammad Nasir Iqbal and Muhammad Irshad

    Citation: BMC Biotechnology 2012 12:46

    Content type: Research article

    Published on:

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