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Protein and enzyme technology

This section will consider studies that focus on recombinant proteins and enzymes, cell-free protein expression systems, and biocatalysis using immobilized enzymes.

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  1. Protein cross-coupling reactions demand high yields, especially if the products are intended for bioanalytics, like enzyme-linked immunosorbent assays. Amongst other factors, the coupling yield depends on the ...

    Authors: Agneta A Prasse, Thomas Zauner, Karin Büttner, Ralf Hoffmann and Thole Zuchner

    Citation: BMC Biotechnology 2014 14:88

    Content type: Research article

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  2. Superparamagnetic iron oxide nanoparticles (MNP) offer several advantages for applications in biomedical and biotechnological research. In particular, MNP-based immobilization of enzymes allows high surface-to...

    Authors: Silvia Sommaruga, Elisabetta Galbiati, Jesus Peñaranda-Avila, Chiara Brambilla, Paolo Tortora, Miriam Colombo and Davide Prosperi

    Citation: BMC Biotechnology 2014 14:82

    Content type: Research article

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  3. Listeriolysin O (LLO) is the main virulence factor of Listeria monocytogenes and facilitates the intracellular survival of the pathogen. Some of its characteristics endorse the growing popularity of LLO for use i...

    Authors: Radosław Stachowiak, Marcin Łyżniak, Maja Grabowska, Katarzyna Roeske, Tomasz Jagielski, Jacek Bielecki, Bożena K Budziszewska, Grażyna Hoser and Jerzy Kawiak

    Citation: BMC Biotechnology 2014 14:77

    Content type: Research article

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  4. Aminoacyl-tRNA synthetases (AARSs) catalyze the first step of protein synthesis. Emerging evidence indicates that AARSs may have additional functions, playing a role in signal transduction pathways regulating ...

    Authors: Yongjiang Lang, Yanling Zhang, Ling Zhan, Zhe Feng, Xiushi Zhou, Min Yu and Wei Mo

    Citation: BMC Biotechnology 2014 14:64

    Content type: Research article

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  5. Inflammatory mediators can serve as biomarkers for the monitoring of the disease progression or prognosis in many conditions. In the present study we introduce an adaptation of a membrane-based technique in wh...

    Authors: Raffaele Altara, Marco Manca, Marleen HM Hessel, Ben J Janssen, Harry H A Struijker-Boudier, Rob JJ Hermans and W Matthijs Blankesteijn

    Citation: BMC Biotechnology 2014 14:63

    Content type: Methodology article

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  6. Pectinases play an important role in plant cell wall deconstruction and have potential in diverse industries such as food, wine, animal feed, textile, paper, fuel, and others. The demand for such enzymes is in...

    Authors: Eridan Orlando Pereira, Igor Kolotilin, Andrew Jonathan Conley and Rima Menassa

    Citation: BMC Biotechnology 2014 14:59

    Content type: Research article

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  7. Cassava starch is considered as a potential source for the commercial production of bioethanol because of its availability and low market price. It can be used as a basic source to support large-scale biologic...

    Authors: Sidra Pervez, Afsheen Aman, Samina Iqbal, Nadir Naveed Siddiqui and Shah Ali Ul Qader

    Citation: BMC Biotechnology 2014 14:49

    Content type: Research article

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  8. Cholesterol oxidases are important enzymes for applications such as the analysis of cholesterol in clinical samples, the synthesis of steroid derived drugs, and are considered as potential antibacterial drug t...

    Authors: Renate Reiss, Greta Faccio, Linda Thöny-Meyer and Michael Richter

    Citation: BMC Biotechnology 2014 14:46

    Content type: Research article

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  9. Lactobacillus species are used as bacterial vectors to deliver functional peptides to the intestine because they are delivered live to the intestine, colonize the mucosal surface, and continue to produce the desi...

    Authors: Yukihiro Hiramatsu, Masatatsu Yamamoto, Tomomitsu Satho, Keiichi Irie, Akiko Kai, Saori Uyeda, Yuki Fukumitsu, Akihisa Toda, Takeshi Miyata, Fumio Miake, Takeshi Arakawa and Nobuhiro Kashige

    Citation: BMC Biotechnology 2014 14:38

    Content type: Research article

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  10. Cell-free protein synthesis is a rapid and efficient method for the production of recombinant proteins. Usage of prokaryotic cell-free extracts often leads to non-functional proteins. Eukaryotic counterparts s...

    Authors: Matthias Buntru, Simon Vogel, Holger Spiegel and Stefan Schillberg

    Citation: BMC Biotechnology 2014 14:37

    Content type: Research article

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  11. Arabinan is an important plant polysaccharide degraded mainly by two hydrolytic enzymes, endo-arabinanase and α-L-arabinofuranosidase. In this study, the characterization and application in arabinan degradatio...

    Authors: Hao Shi, Huaihai Ding, Yingjuan Huang, Liangliang Wang, Yu Zhang, Xun Li and Fei Wang

    Citation: BMC Biotechnology 2014 14:35

    Content type: Research article

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  12. Annexin V, a 35.8 kDa intracellular protein, is a Ca+2- dependent phospholipid binding protein with high affinity to phosphatidylserine (PS), which is a well-known hallmark of apoptosis. Annexin V is a sensitive ...

    Authors: Laura S Marder, Juleane Lunardi, Gaby Renard, Diana C Rostirolla, Guilherme O Petersen, José E S Nunes, Ana Paula D de Souza, Ana Christina de O Dias, Jocelei M Chies, Luiz A Basso, Diógenes S Santos and Cristiano V Bizarro

    Citation: BMC Biotechnology 2014 14:33

    Content type: Research article

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  13. Researchers are looking for biomimetic mineralization of ena/mel to manage dental erosion. This study evaluated biomimetic mineralization of demineralized enamel induced by a synthetic and self-assembled oligo...

    Authors: Quan-Li Li, Tian-Yun Ning, Ying Cao, Wei-bo Zhang, May Lei Mei and Chun Hung Chu

    Citation: BMC Biotechnology 2014 14:32

    Content type: Research article

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  14. Efficient conversion of lignocellulosic biomass to fermentable sugars requires the synergistic action of multiple enzymes; consequently enzyme mixtures must be properly formulated for effective hydrolysis. The...

    Authors: Ajay Badhan, Yuxi Wang, Robert Gruninger, Donald Patton, Justin Powlowski, Adrian Tsang and Tim McAllister

    Citation: BMC Biotechnology 2014 14:31

    Content type: Research article

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  15. There is an increasing interest to seek new enzyme preparations for the development of new products derived from bioprocesses to obtain alternative bio-based materials. In this context, four non-commercial lip...

    Authors: Silvia Cesarini, Belén Infanzón, F I Javier Pastor and Pilar Diaz

    Citation: BMC Biotechnology 2014 14:27

    Content type: Research article

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  16. Forestry residues consisting of softwood are a major lignocellulosic resource for production of liquid biofuels. Scots pine, a commercially important forest tree, was fractionated into seven fractions of chips...

    Authors: Monica Normark, Sandra Winestrand, Torbjörn A Lestander and Leif J Jönsson

    Citation: BMC Biotechnology 2014 14:20

    Content type: Research article

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  17. A useful heterologous production system is required to obtain sufficient amounts of recombinant therapeutic proteins, which are often necessary for chemical characterization and engineering studies on the deve...

    Authors: Michiro Muraki

    Citation: BMC Biotechnology 2014 14:19

    Content type: Research article

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  18. Biotechnological applications of microbial pectate lyases (Pels) in plant fiber processing are considered as environmentally friendly. As such, they become promising substitutes for conventional chemical degum...

    Authors: Xiaoman Li, Huilin Wang, Cheng Zhou, Yanhe Ma, Jian Li and Jiangning Song

    Citation: BMC Biotechnology 2014 14:18

    Content type: Research article

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  19. Metal borides are a class of inorganic solids that is much less known and investigated than for example metal oxides or intermetallics. At the same time it is a highly versatile and interesting class of compou...

    Authors: Martin Ploss, Sandra J Facey, Carina Bruhn, Limor Zemel, Kathrin Hofmann, Robert W Stark, Barbara Albert and Bernhard Hauer

    Citation: BMC Biotechnology 2014 14:12

    Content type: Research article

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  20. The Rho-associated coiled-coil kinase-2 (ROCK2) is an important signaling transducer in the transmission of extracellular signals effecting organization of the actin cytoskeleton. ROCK2 has been implicated in ...

    Authors: Amber L Couzens, R Montgomery Gill and Michael P Scheid

    Citation: BMC Biotechnology 2014 14:2

    Content type: Research article

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  21. Halotolerant bacteria are excellent sources for selecting novel enzymes. Being intrinsically stable and active under high salinities, enzymes from these prokaryotes have evolved to function optimally under ext...

    Authors: Li Xin and Yu Hui-Ying

    Citation: BMC Biotechnology 2013 13:108

    Content type: Research article

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  22. HIV genome is packaged and organized in a conical capsid, which is made up of ~1,500 copies of the viral capsid protein p24 (CA). Being a primary structural component and due to its critical roles in both late...

    Authors: Sin Yeang Teow, Siti Aisyah Mualif, Tasyriq Che Omar, Chew Yik Wei, Narazah Mohd Yusoff and Syed A Ali

    Citation: BMC Biotechnology 2013 13:107

    Content type: Methodology article

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  23. It is well known that the C-terminal zinc-finger-3 in transcription factor Sp1 contributes more than the N-terminal zinc-finger-1 in determining Sp1’s DNA binding capacity. Sp1-like artificial poly-zinc-finger pr...

    Authors: Baozhen Zhang, Shengyan Xiang, Yanru Yin, Liankun Gu and Dajun Deng

    Citation: BMC Biotechnology 2013 13:106

    Content type: Research article

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  24. β-1,3-Glucanases catalyze the hydrolysis of glucan polymers containing β-1,3-linkages. These enzymes are of great biotechnological, agricultural and industrial interest. The applications of β-1,3-glucanases is...

    Authors: Rui Cheng, Jinping Chen, Xiaohong Yu, Yang Wang, Shiming Wang and Jianfa Zhang

    Citation: BMC Biotechnology 2013 13:105

    Content type: Research article

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  25. β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in the functional foods...

    Authors: Niël van Wyk, Kim M Trollope, Emma T Steenkamp, Brenda D Wingfield and Heinrich Volschenk

    Citation: BMC Biotechnology 2013 13:100

    Content type: Research article

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  26. Burkholderia cepacia lipase (BCL) has been proved to be capable of resolution reactions. However, its free form usually exhibits low stability, bad resistance and no reusability, which restrict its further indust...

    Authors: Xiang Li, Shuangshuang Huang, Li Xu and Yunjun Yan

    Citation: BMC Biotechnology 2013 13:92

    Content type: Research article

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  27. Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in direc...

    Authors: Isabel Pardo, Xiomara Chanagá, Ana Isabel Vicente, Miguel Alcalde and Susana Camarero

    Citation: BMC Biotechnology 2013 13:90

    Content type: Research article

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  28. The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus ...

    Authors: Hidenao Arai, Koichi Nishigaki, Naoto Nemoto, Miho Suzuki and Yuzuru Husimi

    Citation: BMC Biotechnology 2013 13:85

    Content type: Research article

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  29. Mannan is one of the primary polysaccharides in hemicellulose and is widely distributed in plants. β-Mannosidase is an important constituent of the mannan-degrading enzyme system and it plays an important role...

    Authors: Hao Shi, Yingjuan Huang, Yu Zhang, Wenqian Li, Xun Li and Fei Wang

    Citation: BMC Biotechnology 2013 13:83

    Content type: Research article

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  30. Phytic acid and phytates can interact with biomolecules, such as proteins and carbohydrates, and are anti-nutritional factors found in food and feed. Therefore, it is necessary to remove these compounds in foo...

    Authors: Ping Yu and Yirun Chen

    Citation: BMC Biotechnology 2013 13:78

    Content type: Research article

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  31. The glutamine synthetase-based protein expression system is widely used in industry and academia for producing recombinant proteins but relies on the cloning of transfected cells, necessitating substantial inv...

    Authors: Rachel Knox, Joanne E Nettleship, Veronica T Chang, Zhao Kun Hui, Ana Mafalda Santos, Nahid Rahman, Ling-Pei Ho, Raymond J Owens and Simon J Davis

    Citation: BMC Biotechnology 2013 13:74

    Content type: Methodology article

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  32. Lactose intolerance is a common health concern causing gastrointestinal symptoms and avoidance of dairy products by afflicted individuals. Since milk is a primary source of calcium and vitamin D, lactose intol...

    Authors: Bin Li, Zemin Wang, Shiwu Li, William Donelan, Xingli Wang, Taixing Cui and Dongqi Tang

    Citation: BMC Biotechnology 2013 13:73

    Content type: Research article

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  33. The detailed understanding of the functions and mechanisms of the actin and microtubuli cytoskeleton depended, besides innovative methods in live cell imaging, on the purification and labeling of its constitue...

    Authors: Christian Renz, Nils Johnsson and Thomas Gronemeyer

    Citation: BMC Biotechnology 2013 13:60

    Content type: Research article

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  34. An eco-friendly treatment of industrial effluents is a major environmental concern of the modern world in the face of stringent environmental legislations. By keeping in mind the extensive industrial applicati...

    Authors: Hafiz Muhammad Nasir Iqbal and Muhammad Asgher

    Citation: BMC Biotechnology 2013 13:56

    Content type: Research article

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  35. Chromobacterium violaceum is a free-living β-proteobacterium found in tropical and subtropical regions. The genomic sequencing of C. violaceum ATCC 12472 has revealed many genes that underpin its adaptability to ...

    Authors: Marina Duarte Pinto Lobo, Fredy Davi Albuquerque Silva, Patrícia Gadelha de Castro Landim, Paloma Ribeiro da Cruz, Thaís Lima de Brito, Suelen Carneiro de Medeiros, José Tadeu Abreu Oliveira, Ilka Maria Vasconcelos, Humberto D’Muniz Pereira and Thalles Barbosa Grangeiro

    Citation: BMC Biotechnology 2013 13:46

    Content type: Research article

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  36. In situ magnetic separation (ISMS) has emerged as a powerful tool to overcome process constraints such as product degradation or inhibition of target production. In the present work, an integrated ISMS process...

    Authors: Martin Cerff, Alexander Scholz, Matthias Franzreb, Iris L Batalha, Ana Cecilia A Roque and Clemens Posten

    Citation: BMC Biotechnology 2013 13:44

    Content type: Research article

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  37. The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconsti...

    Authors: Ashley R Long, Catherine C O’Brien, Ketan Malhotra, Christine T Schwall, Arlene D Albert, Anthony Watts and Nathan N Alder

    Citation: BMC Biotechnology 2013 13:41

    Content type: Research article

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  38. Basidiomycete high-redox potential laccases (HRPLs) working in human physiological fluids (pH 7.4, 150 mM NaCl) arise great interest in the engineering of 3D-nanobiodevices for biomedical uses. In two previous...

    Authors: Diana M Mate, David Gonzalez-Perez, Roman Kittl, Roland Ludwig and Miguel Alcalde

    Citation: BMC Biotechnology 2013 13:38

    Content type: Research article

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  39. Human α-synuclein is a small-sized, natively unfolded protein that in fibrillar form is the primary component of Lewy bodies, the pathological hallmark of Parkinson’s disease. Experimental evidence suggests th...

    Authors: Laura Caldinelli, Diego Albani and Loredano Pollegioni

    Citation: BMC Biotechnology 2013 13:32

    Content type: Research article

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  40. The conventional degumming process of ramie with alkaline treatment at high temperature causes severe environmental pollution. Pectate lyases can be used to remove pectin from ramie in a degumming process with...

    Authors: Chengjie Zhang, Jia Yao, Cheng Zhou, Liangwei Mao, Guimin Zhang and Yanhe Ma

    Citation: BMC Biotechnology 2013 13:26

    Content type: Research article

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  41. VanYn, encoded by the dbv7 gene (also known as vanY n ) of the biosynthetic cluster devoted to A40926 production, is a novel protein involved in the mechanism of self-resista...

    Authors: Elisa Binda, Giorgia Letizia Marcone, Francesca Berini, Loredano Pollegioni and Flavia Marinelli

    Citation: BMC Biotechnology 2013 13:24

    Content type: Research article

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  42. Cold-active enzymes, sourced from cold-adapted organisms, are characterized by high catalytic efficiencies at low temperatures compared with their mesophilic counterparts, which have poor activity. This proper...

    Authors: Anna Wierzbicka-Woś, Paulina Bartasun, Hubert Cieśliński and Józef Kur

    Citation: BMC Biotechnology 2013 13:22

    Content type: Research article

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  43. Tk-SP is a member of subtilisin-like serine proteases from a hyperthermophilic archaeon Thermococcus kodakarensis. It has been known that the hyper-stable protease, Tk-SP, could exhibit enzymatic activity even at...

    Authors: Azumi Hirata, Yuki Hori, Yuichi Koga, Jun Okada, Akikazu Sakudo, Kazuyoshi Ikuta, Shigenori Kanaya and Kazufumi Takano

    Citation: BMC Biotechnology 2013 13:19

    Content type: Research article

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  44. α–glucosidase (HBGase) plays a key role in hydrolyzing α-glucosidic linkages. In Apis mellifera, three isoforms of HBGase (I, II and III) have been reported, which differ in their nucleotide composition, encoding...

    Authors: Jirattikarn Kaewmuangmoon, Manlika Kilaso, Ubolsree Leartsakulpanich, Kiyoshi Kimura, Atsuo Kimura and Chanpen Chanchao

    Citation: BMC Biotechnology 2013 13:16

    Content type: Research article

    Published on:

  45. Hair is composed mainly of keratin protein and a small amount of lipid. Protein hydrolysates, in particular those with low molecular weight distribution have been known to protect hair against chemical and env...

    Authors: Ana Lúcia Vazquez Villa, Márcia Regina Senrra Aragão, Elisabete Pereira dos Santos, Ana Maria Mazotto, Russolina B Zingali, Edilma Paraguai de Souza and Alane Beatriz Vermelho

    Citation: BMC Biotechnology 2013 13:15

    Content type: Research article

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  46. A nitrilase-mediated pathway has significant advantages in the production of optically pure (R)-(−)-mandelic acid. However, unwanted byproduct, low enantioselectivity, and specific activity reduce its value in...

    Authors: Hualei Wang, Huihui Sun and Dongzhi Wei

    Citation: BMC Biotechnology 2013 13:14

    Content type: Research article

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