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Protein and enzyme technology

This section will consider studies that focus on recombinant proteins and enzymes, cell-free protein expression systems, and biocatalysis using immobilized enzymes.

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  1. Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are su...

    Authors: Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu and Rijun Zhang

    Citation: BMC Biotechnology 2017 17:55

    Content type: Research Article

    Published on:

  2. Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Authors: Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    Citation: BMC Biotechnology 2017 17:37

    Content type: Research article

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  3. Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k ...

    Authors: Qing Wang, Luyao Bao, Chenjun Jia, Mei Li, Jian-Jun Li and Xuefeng Lu

    Citation: BMC Biotechnology 2017 17:31

    Content type: Research article

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  4. Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and b...

    Authors: Gabe Javitt, Zohar Ben-Barak-Zelas, Moran Jerabek-Willemsen and Ayelet Fishman

    Citation: BMC Biotechnology 2017 17:23

    Content type: Research article

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  5. α-Amylase plays a pivotal role in a broad range of industrial processes. To meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. Howe...

    Authors: Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, Siti Aisyah Alias, Bei Gao and Dongzhi Wei

    Citation: BMC Biotechnology 2017 17:22

    Content type: Research article

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  6. Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability...

    Authors: Lin Ge, Anna Chen, Jianjun Pei, Linguo Zhao, Xianying Fang, Gang Ding, Zhenzhong Wang, Wei Xiao and Feng Tang

    Citation: BMC Biotechnology 2017 17:21

    Content type: Research article

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  7. Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-pol...

    Authors: Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua and Duriya Chantasingh

    Citation: BMC Biotechnology 2017 17:15

    Content type: Research article

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  8. Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As...

    Authors: Aiswarya Chenthamarakshan, Nayana Parambayil, Nafeesathul Miziriya, P. S. Soumya, M. S. Kiran Lakshmi, Anala Ramgopal, Anuja Dileep and Padma Nambisan

    Citation: BMC Biotechnology 2017 17:12

    Content type: Research article

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  9. Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affi...

    Authors: Marco Biancucci, Jazel S. Dolores, Jennifer Wong, Sarah Grimshaw, Wayne F. Anderson, Karla J. F. Satchell and Keehwan Kwon

    Citation: BMC Biotechnology 2017 17:1

    Content type: Methodology article

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  10. Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their compo...

    Authors: Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana and Verawat Champreda

    Citation: BMC Biotechnology 2016 16:82

    Content type: Research article

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  11. Hexuronic acids such as D-galacturonic acid and D-glucuronic acid can be utilized via different pathways within the metabolism of microorganisms. One representative, the oxidative pathway, generates α-keto-glu...

    Authors: André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    Citation: BMC Biotechnology 2016 16:80

    Content type: Research article

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  12. Family 11 alkaline xylanases have great potential economic applications in the pulp and paper industry. In this study, we would improve the alkalophilicity of family 11 alkaline xylanase Xyn11A-LC from Bacillus s...

    Authors: Wenqin Bai, Yufan Cao, Jun Liu, Qinhong Wang and Zhenhu Jia

    Citation: BMC Biotechnology 2016 16:77

    Content type: Research article

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  13. Transglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptom...

    Authors: Song Liu, Miao Wang, Guocheng Du and Jian Chen

    Citation: BMC Biotechnology 2016 16:75

    Content type: Research article

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  14. Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan. Appended ...

    Authors: Shiping Liu and Shaojun Ding

    Citation: BMC Biotechnology 2016 16:73

    Content type: Research article

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  15. A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of ...

    Authors: Guojun Wang, Ting Zhang, Haifeng Huang, Shurong Hou, Xiabin Chen, Fang Zheng and Chang-Guo Zhan

    Citation: BMC Biotechnology 2016 16:72

    Content type: Research article

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  16. Ethyl (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE) is a versatile chiral precursor for many pharmaceuticals. Although several biosynthesis strategies have been documented to convert ethyl 4-chloro-3-oxobutanoate (CO...

    Authors: Xiang Chen, Zhi-Qiang Liu, Chao-Ping Lin and Yu-Guo Zheng

    Citation: BMC Biotechnology 2016 16:70

    Content type: Research article

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  17. Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is ...

    Authors: Alexander Falkenhagen, Sabah Asad, Stanley E. Read and Sadhna Joshi

    Citation: BMC Biotechnology 2016 16:66

    Content type: Methodology article

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  18. Targeted therapies for cancer, especially the malignant cancer, are always restricted by the deficiency of tumor-specific drug delivery methods. Subtilase cytotoxic is a virulent cytotoxin, and the subunit A (...

    Authors: Lichao Zhang, Zongwei Li, Tonglin Shi, Xiaoqin La, Hanqing Li and Zhuoyu Li

    Citation: BMC Biotechnology 2016 16:65

    Content type: Research article

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  19. L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. In previous reports, AIs from thermophilic bacterial strains had been wildly researched, but the brownin...

    Authors: Wending Mei, Lu Wang, Ying Zang, Zhaojuan Zheng and Jia Ouyang

    Citation: BMC Biotechnology 2016 16:55

    Content type: Research article

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  20. Dioxins are one of the most toxic groups of persistent organic pollutants. Their bioaccumulation through the food chain constitutes a potential risk for human health. Upon cell entry, dioxins bind specifically...

    Authors: Walaa Faiad, Abdulsamie Hanano, Mohamed Maher Kabakibi and Abdul Qader Abbady

    Citation: BMC Biotechnology 2016 16:51

    Content type: Research article

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  21. Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric pathogen of swine. The spike glycoprotein (S) of PEDV is the major immunogenic determinant that plays a pivotal role in the induction of ne...

    Authors: Da-Chuan Piao, Do-Woon Shin, In-Seon Kim, Hui-Shan Li, Seo-Ho Oh, Bijay Singh, S. Maharjan, Yoon-Seok Lee, Jin-Duck Bok, Chong-Su Cho, Zhong-Shan Hong, Sang-Kee Kang and Yun-Jaie Choi

    Citation: BMC Biotechnology 2016 16:39

    Content type: Research article

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  22. The whole-cell lipase from Burkholderia cepacia has been used as a biocatalyst in organic synthesis. However, there is no report in the literature on the component or the gene sequence of the cell-bound lipase fr...

    Authors: Zhengyu Shu, Hong Lin, Shaolei Shi, Xiangduo Mu, Yanru Liu and Jianzhong Huang

    Citation: BMC Biotechnology 2016 16:38

    Content type: Research article

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  23. Angiotensinogen (ANG) is a macromolecular precursor of angiotensin, which regulates blood pressure and electrolyte balance. ANG is specifically cleaved by renin, an aspartic protease, to initiate the angiotens...

    Authors: Shinji Yamashita, Naoya Shibata, Akiyoshi Boku-Ikeda, Erika Abe, Ayumi Inayama, Takashi Yamaguchi, Ayano Higuma, Kaoru Inagaki, Tomoyo Tsuyuzaki, Satoshi Iwamoto, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Kazal Boron Biswas, A. H. M. Nurun Nabi, Tsutomu Nakagawa…

    Citation: BMC Biotechnology 2016 16:33

    Content type: Research article

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  24. The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other protein) has found widespread application in basic re...

    Authors: Dona York, Jeanne Baker, Patrick G. Holder, Lesley C. Jones, Penelope M. Drake, Robyn M. Barfield, Gregory T. Bleck and David Rabuka

    Citation: BMC Biotechnology 2016 16:23

    Content type: Research article

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  25. The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from

    Authors: Weifeng Sun, Longbao Zhu, Xianggui Chen, Ping Chen, Lingling Yang, Wenwu Ding, Zhemin Zhou and Yi Liu

    Citation: BMC Biotechnology 2016 16:21

    Content type: Research article

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  26. Aspergillus fumigatus R1 produced xylanase under submerged fermentation which degrades the complex hemicelluloses contained in agricultural substrates. Xylanases have gained considerab...

    Authors: Rehan Ahmed Deshmukh, Sharmili Jagtap, Madan Kumar Mandal and Suraj Kumar Mandal

    Citation: BMC Biotechnology 2016 16:11

    Content type: Research article

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  27. CD40, also called Bp50, is a novel member of the TNF receptor superfamily. Based on its important role in multiple physiological and pathological processes, the CD40 signaling pathway has become a vital target...

    Authors: Yu Zhan, Yilei Wei, Pengfei Chen, Haohao Zhang, Dandan Liu, Jie Zhang, Rongzeng Liu, Ran Chen, Jun Zhang, Wei Mo and Xiaoren Zhang

    Citation: BMC Biotechnology 2016 16:8

    Content type: Research article

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  28. Resistin (Retn) is a cytokine which has a controversial physiological role regarding its involvement with obesity and type II diabetes mellitus. Recently, murine Retn was found to be a possibly potential regul...

    Authors: Fangyuan Wang, Jin Gao, Alyssa Malisani, Xiaowei Xi, Wei Han and Xiaoping Wan

    Citation: BMC Biotechnology 2015 15:105

    Content type: Methodology article

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  29. Thermal stable α-glucosidases with transglycosylation activity could be applied to the industrial production of oligosaccharides as well as conjugation of sugars to biologically useful materials. Therefore, α-...

    Authors: Cheng Zhou, Yanfen Xue and Yanhe Ma

    Citation: BMC Biotechnology 2015 15:97

    Content type: Research article

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  30. Pullulanase is an important debranching enzyme and has been widely utilized to hydrolyse the α-1,6 glucosidic linkages in starch/sugar industry. Selecting new bacterial strains or improving bacterial strains i...

    Authors: Wei Wei, Jing Ma, Si-Qi Chen, Xiang-Hai Cai and Dong-Zhi Wei

    Citation: BMC Biotechnology 2015 15:96

    Content type: Research article

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  31. Amidation of the carboxyl terminal of many peptides is essential for full biological potency, often increasing receptor binding and stability. The single enzyme responsible for this reaction is peptidylglycine...

    Authors: Kristina Carlson, Steven C. Pomerantz, Omid Vafa, Michael Naso, William Strohl, Richard E. Mains and Betty A. Eipper

    Citation: BMC Biotechnology 2015 15:95

    Content type: Research article

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  32. Recombinant factor VII (rFVII), the precursor molecule for recombinant activated FVII (rFVIIa), is, due to its need for complex post translational modifications, produced in mammalian cells. To evaluate the su...

    Authors: Ernst Böhm, Birgit K. Seyfried, Michael Dockal, Michael Graninger, Meinhard Hasslacher, Marianne Neurath, Christian Konetschny, Peter Matthiessen, Artur Mitterer and Friedrich Scheiflinger

    Citation: BMC Biotechnology 2015 15:87

    Content type: Research article

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  33. Immobilization of enzymes onto different carriers increases enzyme’s stability and reusability within biotechnological and pharmaceutical applications. However, some immobilization techniques are associated wi...

    Authors: Julia Stolarow, Manuel Heinzelmann, Wladimir Yeremchuk, Christoph Syldatk and Rudolf Hausmann

    Citation: BMC Biotechnology 2015 15:77

    Content type: Research article

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  34. Transglutaminases (TGase), synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in food industry. Due to the important applications of TGase in food industry, obtaining robust and food-...

    Authors: Song Liu, Dan Wan, Miao Wang, Catherine Madzak, Guocheng Du and Jian Chen

    Citation: BMC Biotechnology 2015 15:75

    Content type: Research article

    Published on:

  35. Antimicrobial peptides have become important candidates as new antibiotics against resistant bacterial strains. However, the major industrial manufacture of antimicrobial peptides is chemical synthesis with hi...

    Authors: Tonghui Yi, Shiyu Sun, Yibing Huang and Yuxin Chen

    Citation: BMC Biotechnology 2015 15:69

    Content type: Research article

    Published on:

  36. L-ornithine (L-Orn), is an intermediate metabolite in the urea cycle that plays a significant role in humans. L-Orn can be obtained from the catalysis of L-arginine (L-Arg) by arginase. The Pichia pastoris expres...

    Authors: Xue Zhang, Jin Liu, Xianhong Yu, Fei Wang, Li Yi, Zhezhe Li, Yunyun Liu and Lixin Ma

    Citation: BMC Biotechnology 2015 15:66

    Content type: Research article

    Published on:

  37. Trypsinogen is the inactive precursor of trypsin, a serine protease that cleaves proteins and peptides after arginine and lysine residues. In this study, human trypsinogen was used as a model protein to study ...

    Authors: Karin Buettner, Thomas Kreisig, Norbert Sträter and Thole Zuchner

    Citation: BMC Biotechnology 2014 14:109

    Content type: Research article

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  38. Fungal amylase, mainly constitute of fungal α-amylase and glucoamylase, are utilized in a broad range of industries, such as starch hydrolysis, food and brewing. Although various amylases have been found in fu...

    Authors: Zhenggui He, Lujia Zhang, Youzhi Mao, Jingchao Gu, Qi Pan, Sixing Zhou, Bei Gao and Dongzhi Wei

    Citation: BMC Biotechnology 2014 14:114

    Content type: Research article

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  39. Derivatized celluloses, such as methylcellulose (MC) and hydroxypropyl methylcellulose (HPMC), are of pharmaceutical importance and extensively employed in tablet matrices. Each batch of derivatized cellulose ...

    Authors: Laura von Schantz, Herje Schagerlöf, Eva Nordberg Karlsson and Mats Ohlin

    Citation: BMC Biotechnology 2014 14:113

    Content type: Research article

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  40. Recombinant hemagglutinin (rHA) is the active component in Flublok®; a trivalent influenza vaccine produced using the baculovirus expression vector system (BEVS). HA is a membrane bound homotrimer in the influ...

    Authors: Kathleen M Holtz, Pamela S Robinson, Erin E Matthews, Yoshifumi Hashimoto, Clifton E McPherson, Nikolai Khramtsov, Michael J Reifler, Jamal Meghrous, David G Rhodes, Manon M Cox and Indresh K Srivastava

    Citation: BMC Biotechnology 2014 14:111

    Content type: Research article

    Published on:

  41. Xylanase can replace chemical additives to improve the volume and sensory properties of bread in the baking. Suitable baking xylanase with improved yield will promote the application of xylanase in baking indu...

    Authors: Fei Xiang Zhan, Qin Hong Wang, Si Jing Jiang, Yu Ling Zhou, Gui Min Zhang and Yan He Ma

    Citation: BMC Biotechnology 2014 14:107

    Content type: Research article

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  42. The enzyme cellobiose dehydrogenase (CDH) can be used to oxidize lactose to lactobionic acid. As Sclerotium rolfsii is known to be a good producer of CDH, the aim of this paper was to simplify its production and ...

    Authors: Christin Fischer, Annett Krause and Thomas Kleinschmidt

    Citation: BMC Biotechnology 2014 14:97

    Content type: Research article

    Published on:

  43. This study compared the performance of five commercially available kits in extracting total RNA from small eukaryotic tissue samples (<15 mg). Total RNA was isolated from fathead minnow (Pimephales promelas) tiss...

    Authors: Marlo K Sellin Jeffries, Andor J Kiss, Austin W Smith and James T Oris

    Citation: BMC Biotechnology 2014 14:94

    Content type: Methodology article

    Published on:

  44. Neurotrophic factors influence survival, differentiation, proliferation and death of neuronal cells within the central nervous system. Human ciliary neurotrophic factor (hCNTF) has neuroprotective properties a...

    Authors: Jaakko M Itkonen, Arto Urtti, Louise E Bird and Sanjay Sarkhel

    Citation: BMC Biotechnology 2014 14:92

    Content type: Research article

    Published on:

  45. Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1, 4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and has a number of applications in differe...

    Authors: Hanpeng Liao, Shuixian Li, Haiping Zheng, Zhong Wei, Dongyang Liu, Waseem Raza, Qirong Shen and Yangchun Xu

    Citation: BMC Biotechnology 2014 14:90

    Content type: Research article

    Published on:

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