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Protein and enzyme technology

Section edited by Ayelet Fishman

This section will consider studies that focus on recombinant proteins and enzymes, cell-free protein expression systems, and biocatalysis using immobilized enzymes.

Page 1 of 3

  1. Content type: Research article

    Ricin A chain (RTA) and Pokeweed antiviral proteins (PAPs) are plant-derived N-glycosidase ribosomal-inactivating proteins (RIPs) isolated from Ricinus communis and Phytolacca Americana respectively. This study w...

    Authors: Yasser Hassan, Sherry Ogg and Hui Ge

    Citation: BMC Biotechnology 2018 18:47

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  2. Content type: Research article

    The bacterial nicotine-degrading enzyme NicA2 isolated from P. putida was studied to assess its potential use in the treatment of tobacco dependence.

    Authors: Paul R. Pentel, Michael D. Raleigh, Mark G. LeSage, Thomas Thisted, Stephen Horrigan, Zuzana Biesova and Matthew W. Kalnik

    Citation: BMC Biotechnology 2018 18:46

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  3. Content type: Research article

    Cellulose is the most important component of lignocellulose, and its degradation requires three different types of enzymes to act synergistically. There have been reports of single gene duality, but no gene ha...

    Authors: Zi-Lu Liu, Hua-Nan Li, Hui-Ting Song, Wen-Jing Xiao, Wu-Cheng Xia, Xiao-Peng Liu and Zheng-Bing Jiang

    Citation: BMC Biotechnology 2018 18:43

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  4. Content type: Research article

    Tetanus neurotoxin (TeNT) is taken up at nerve terminals and undergoes retrograde migration. The toxic properties of TeNT reside in the toxin light chain (L), but like complete TeNT, the TeNT heavy chain (TTH)...

    Authors: Yasuhiro Watanabe, Takashi Matsuba, Mami Nakanishi, Mio Une, Ritsuko Hanajima and Kenji Nakashima

    Citation: BMC Biotechnology 2018 18:39

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  5. Content type: Research article

    Cellulases are of great significance for full utilization of lignocellulosic biomass. Termites have an efficient ability to degrade cellulose. Heterologous production of the termite-origin cellulases is the fi...

    Authors: Pengfei Zhang, Xianghua Yuan, Yuguang Du and Jian-Jun Li

    Citation: BMC Biotechnology 2018 18:35

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  6. Content type: Research article

    Phytase supplied in feeds for monogastric animals is important for improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability to...

    Authors: Nanyu Han, Huabiao Miao, Tingting Yu, Bo Xu, Yunjuan Yang, Qian Wu, Rui Zhang and Zunxi Huang

    Citation: BMC Biotechnology 2018 18:36

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  7. Content type: Research article

    Dihydro-β-ionone is a principal aroma compound and has received considerable attention by flavor and fragrance industry. The traditional method of preparing dihydro-β-ionone has many drawbacks, which has restr...

    Authors: Xuesong Zhang, Shiyong Liao, Fuliang Cao, Linguo Zhao, Jianjun Pei and Feng Tang

    Citation: BMC Biotechnology 2018 18:26

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  8. Content type: Research article

    Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive ...

    Authors: Danping Zhang, Chengjie Xie, Rongzhi Wang, Qinghai Yang, Huiling Chen, Sumei Ling, Shihua Wang and Kunzhi Jia

    Citation: BMC Biotechnology 2018 18:25

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  9. Content type: Research article

    Hirudin is a potent thrombin inhibitor but its antithrombotic properties are offset by bleeding side-effects. Because hirudin’s N-terminus must engage thrombin’s active site for effective inhibition, fusing a ...

    Authors: William P. Sheffield, Louise J. Eltringham-Smith and Varsha Bhakta

    Citation: BMC Biotechnology 2018 18:21

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  10. Content type: Methodology article

    Recombinant production of amebic cysteine proteases using Escherichia coli cells as the bacterial system has become a challenging effort, with protein insolubility being the most common issue. Since many of these...

    Authors: Ekaterina Jalomo-Khayrova, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Ignacio A. Rivero and Marco A. Ramos

    Citation: BMC Biotechnology 2018 18:20

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  11. Content type: Research article

    Safe and effective hemostatic materials are important for reducing mortality resulting from excessive hemorrhage. In this work, new biomaterials with hemostatic effects were created by fusing the gene coding f...

    Authors: Shasha Yang, Sili Wei, Yun Mao, Hanxue Zheng, Juantao Feng, Jihong Cui, Xin Xie, Fulin Chen and Honmgmin Li

    Citation: BMC Biotechnology 2018 18:12

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  12. Content type: Research article

    The recently developed genetically encoded calcium indicator (GECI), called NTnC, has a novel design with reduced size due to utilization of the troponin C (TnC) as a Ca2+-binding moiety inserted into the mNeonGr...

    Authors: D. A. Doronin, N. V. Barykina, O. M. Subach, V. P. Sotskov, V. V. Plusnin, O. A. Ivleva, E. A. Isaakova, A. M. Varizhuk, G. E. Pozmogova, A. Y. Malyshev, I. V. Smirnov, K. D. Piatkevich, K. V. Anokhin, G. N. Enikolopov and F. V. Subach

    Citation: BMC Biotechnology 2018 18:10

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  13. Content type: Research article

    The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and...

    Authors: Anil S. Prajapati, Vishakha A. Pawar, Ketankumar J. Panchal, Ankit P. Sudhir, Bhaumik R. Dave, Darshan H. Patel and R. B. Subramanian

    Citation: BMC Biotechnology 2018 18:9

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  14. Content type: Research article

    Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products depending on the regioselectivity of the immobilized lipase biocatalyst

    Authors: Erick Abreu Silveira, Sonia Moreno-Perez, Alessandra Basso, Simona Serban, Rita Pestana Mamede, Paulo W. Tardioli, Cristiane Sanchez Farinas, Javier Rocha-Martin, Gloria Fernandez-Lorente and Jose M. Guisan

    Citation: BMC Biotechnology 2017 17:88

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  15. Content type: Research article

    Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO4], [Cl], [MeCO2]) were...

    Authors: Zhao Wang, John Gräsvik, Leif J. Jönsson and Sandra Winestrand

    Citation: BMC Biotechnology 2017 17:82

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  16. Content type: Research article

    The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus ...

    Authors: Andris Kazaks, I-Na Lu, Sophie Farinelle, Alex Ramirez, Vincenzo Crescente, Benjamin Blaha, Olotu Ogonah, Tarit Mukhopadhyay, Mapi Perez de Obanos, Alejandro Krimer, Inara Akopjana, Janis Bogans, Velta Ose, Anna Kirsteina, Tatjana Kazaka, Nicola J. Stonehouse…

    Citation: BMC Biotechnology 2017 17:79

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  17. Content type: Research article

    Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and antiba...

    Authors: Natalia Inés Almasia, María Paula Molinari, Guillermo Andrés Maroniche, Vanesa Nahirñak, María Pilar Barrios Barón, Oscar Alberto Taboga and Cecilia Vazquez Rovere

    Citation: BMC Biotechnology 2017 17:75

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  18. Content type: Research article

    Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed...

    Authors: Aldricho Alpha Pollardo, Hong-shik Lee, Dohoon Lee, Sangyong Kim and Jaehoon Kim

    Citation: BMC Biotechnology 2017 17:70

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  19. Content type: Research article

    Fibroblast growth factor 21 (FGF21) is an endocrine-acting hormone that has the potential to treat diabetic nephropathy. However, development of FGF21 into a therapeutic has been hindered due to its low intrin...

    Authors: Longwei Zhao, Huiyan Wang, Junjun Xie, Zilu Chen, Xiaokun Li and Jianlou Niu

    Citation: BMC Biotechnology 2017 17:58

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  20. Content type: Research article

    Fas ligand plays a key role in the human immune system as a major cell death inducing protein. The extracellular domain of human Fas ligand (hFasLECD) triggers apoptosis of malignant cells, and therefore is ex...

    Authors: Michiro Muraki and Kiyonori Hirota

    Citation: BMC Biotechnology 2017 17:56

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  21. Content type: Research article

    Effective and simple methods that lead to higher enzymatic efficiencies are highly sough. Here we proposed a foldon-triggered trimerization of the target enzymes with significantly improved catalytic performan...

    Authors: Xinzhe Wang, Huihua Ge, Dandan Zhang, Shuyu Wu and Guangya Zhang

    Citation: BMC Biotechnology 2017 17:57

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  22. Content type: Research Article

    Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are su...

    Authors: Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu and Rijun Zhang

    Citation: BMC Biotechnology 2017 17:55

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  23. Content type: Research article

    Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Authors: Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    Citation: BMC Biotechnology 2017 17:37

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  24. Content type: Research article

    Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k ...

    Authors: Qing Wang, Luyao Bao, Chenjun Jia, Mei Li, Jian-Jun Li and Xuefeng Lu

    Citation: BMC Biotechnology 2017 17:31

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  25. Content type: Research article

    Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and b...

    Authors: Gabe Javitt, Zohar Ben-Barak-Zelas, Moran Jerabek-Willemsen and Ayelet Fishman

    Citation: BMC Biotechnology 2017 17:23

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  26. Content type: Research article

    α-Amylase plays a pivotal role in a broad range of industrial processes. To meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. Howe...

    Authors: Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, Siti Aisyah Alias, Bei Gao and Dongzhi Wei

    Citation: BMC Biotechnology 2017 17:22

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  27. Content type: Research article

    Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability...

    Authors: Lin Ge, Anna Chen, Jianjun Pei, Linguo Zhao, Xianying Fang, Gang Ding, Zhenzhong Wang, Wei Xiao and Feng Tang

    Citation: BMC Biotechnology 2017 17:21

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  28. Content type: Research article

    Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-pol...

    Authors: Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua and Duriya Chantasingh

    Citation: BMC Biotechnology 2017 17:15

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  29. Content type: Research article

    Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As...

    Authors: Aiswarya Chenthamarakshan, Nayana Parambayil, Nafeesathul Miziriya, P. S. Soumya, M. S. Kiran Lakshmi, Anala Ramgopal, Anuja Dileep and Padma Nambisan

    Citation: BMC Biotechnology 2017 17:12

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  30. Content type: Methodology article

    Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affi...

    Authors: Marco Biancucci, Jazel S. Dolores, Jennifer Wong, Sarah Grimshaw, Wayne F. Anderson, Karla J. F. Satchell and Keehwan Kwon

    Citation: BMC Biotechnology 2017 17:1

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  31. Content type: Research article

    Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their compo...

    Authors: Wanwitoo Wanmolee, Warasirin Sornlake, Nakul Rattanaphan, Surisa Suwannarangsee, Navadol Laosiripojana and Verawat Champreda

    Citation: BMC Biotechnology 2016 16:82

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  32. Content type: Research article

    Hexuronic acids such as D-galacturonic acid and D-glucuronic acid can be utilized via different pathways within the metabolism of microorganisms. One representative, the oxidative pathway, generates α-keto-glu...

    Authors: André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    Citation: BMC Biotechnology 2016 16:80

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  33. Content type: Research article

    Family 11 alkaline xylanases have great potential economic applications in the pulp and paper industry. In this study, we would improve the alkalophilicity of family 11 alkaline xylanase Xyn11A-LC from Bacillus s...

    Authors: Wenqin Bai, Yufan Cao, Jun Liu, Qinhong Wang and Zhenhu Jia

    Citation: BMC Biotechnology 2016 16:77

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  34. Content type: Research article

    Transglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptom...

    Authors: Song Liu, Miao Wang, Guocheng Du and Jian Chen

    Citation: BMC Biotechnology 2016 16:75

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  35. Content type: Research article

    Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan. Appended ...

    Authors: Shiping Liu and Shaojun Ding

    Citation: BMC Biotechnology 2016 16:73

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  36. Content type: Research article

    A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of ...

    Authors: Guojun Wang, Ting Zhang, Haifeng Huang, Shurong Hou, Xiabin Chen, Fang Zheng and Chang-Guo Zhan

    Citation: BMC Biotechnology 2016 16:72

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  37. Content type: Research article

    Ethyl (R)-4-chloro-3-hydroxybutyrate ((R)-CHBE) is a versatile chiral precursor for many pharmaceuticals. Although several biosynthesis strategies have been documented to convert ethyl 4-chloro-3-oxobutanoate (CO...

    Authors: Xiang Chen, Zhi-Qiang Liu, Chao-Ping Lin and Yu-Guo Zheng

    Citation: BMC Biotechnology 2016 16:70

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  38. Content type: Methodology article

    Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is ...

    Authors: Alexander Falkenhagen, Sabah Asad, Stanley E. Read and Sadhna Joshi

    Citation: BMC Biotechnology 2016 16:66

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  39. Content type: Research article

    Targeted therapies for cancer, especially the malignant cancer, are always restricted by the deficiency of tumor-specific drug delivery methods. Subtilase cytotoxic is a virulent cytotoxin, and the subunit A (...

    Authors: Lichao Zhang, Zongwei Li, Tonglin Shi, Xiaoqin La, Hanqing Li and Zhuoyu Li

    Citation: BMC Biotechnology 2016 16:65

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  40. Content type: Research article

    L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. In previous reports, AIs from thermophilic bacterial strains had been wildly researched, but the brownin...

    Authors: Wending Mei, Lu Wang, Ying Zang, Zhaojuan Zheng and Jia Ouyang

    Citation: BMC Biotechnology 2016 16:55

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  41. Content type: Research article

    Dioxins are one of the most toxic groups of persistent organic pollutants. Their bioaccumulation through the food chain constitutes a potential risk for human health. Upon cell entry, dioxins bind specifically...

    Authors: Walaa Faiad, Abdulsamie Hanano, Mohamed Maher Kabakibi and Abdul Qader Abbady

    Citation: BMC Biotechnology 2016 16:51

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  42. Content type: Research article

    Porcine epidemic diarrhea virus (PEDV) is a highly contagious enteric pathogen of swine. The spike glycoprotein (S) of PEDV is the major immunogenic determinant that plays a pivotal role in the induction of ne...

    Authors: Da-Chuan Piao, Do-Woon Shin, In-Seon Kim, Hui-Shan Li, Seo-Ho Oh, Bijay Singh, S. Maharjan, Yoon-Seok Lee, Jin-Duck Bok, Chong-Su Cho, Zhong-Shan Hong, Sang-Kee Kang and Yun-Jaie Choi

    Citation: BMC Biotechnology 2016 16:39

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  43. Content type: Research article

    The whole-cell lipase from Burkholderia cepacia has been used as a biocatalyst in organic synthesis. However, there is no report in the literature on the component or the gene sequence of the cell-bound lipase fr...

    Authors: Zhengyu Shu, Hong Lin, Shaolei Shi, Xiangduo Mu, Yanru Liu and Jianzhong Huang

    Citation: BMC Biotechnology 2016 16:38

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  44. Content type: Research article

    Angiotensinogen (ANG) is a macromolecular precursor of angiotensin, which regulates blood pressure and electrolyte balance. ANG is specifically cleaved by renin, an aspartic protease, to initiate the angiotens...

    Authors: Shinji Yamashita, Naoya Shibata, Akiyoshi Boku-Ikeda, Erika Abe, Ayumi Inayama, Takashi Yamaguchi, Ayano Higuma, Kaoru Inagaki, Tomoyo Tsuyuzaki, Satoshi Iwamoto, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Kazal Boron Biswas, A. H. M. Nurun Nabi, Tsutomu Nakagawa…

    Citation: BMC Biotechnology 2016 16:33

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  45. Content type: Research article

    The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other protein) has found widespread application in basic re...

    Authors: Dona York, Jeanne Baker, Patrick G. Holder, Lesley C. Jones, Penelope M. Drake, Robyn M. Barfield, Gregory T. Bleck and David Rabuka

    Citation: BMC Biotechnology 2016 16:23

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  46. Content type: Research article

    The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from

    Authors: Weifeng Sun, Longbao Zhu, Xianggui Chen, Ping Chen, Lingling Yang, Wenwu Ding, Zhemin Zhou and Yi Liu

    Citation: BMC Biotechnology 2016 16:21

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