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Protein and enzyme technology

Section edited by Ayelet Fishman

This section will consider studies that focus on recombinant proteins and enzymes, cell-free protein expression systems, and biocatalysis using immobilized enzymes.

Page 1 of 7
  1. Content type: Research article

    Dihydro-β-ionone is a principal aroma compound and has received considerable attention by flavor and fragrance industry. The traditional method of preparing dihydro-β-ionone has many drawbacks, which has restr...

    Authors: Xuesong Zhang, Shiyong Liao, Fuliang Cao, Linguo Zhao, Jianjun Pei and Feng Tang

    Citation: BMC Biotechnology 2018 18:26

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  2. Content type: Research article

    Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive ...

    Authors: Danping Zhang, Chengjie Xie, Rongzhi Wang, Qinghai Yang, Huiling Chen, Sumei Ling, Shihua Wang and Kunzhi Jia

    Citation: BMC Biotechnology 2018 18:25

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  3. Content type: Research article

    Hirudin is a potent thrombin inhibitor but its antithrombotic properties are offset by bleeding side-effects. Because hirudin’s N-terminus must engage thrombin’s active site for effective inhibition, fusing a ...

    Authors: William P. Sheffield, Louise J. Eltringham-Smith and Varsha Bhakta

    Citation: BMC Biotechnology 2018 18:21

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  4. Content type: Methodology article

    Recombinant production of amebic cysteine proteases using Escherichia coli cells as the bacterial system has become a challenging effort, with protein insolubility being the most common issue. Since many of these...

    Authors: Ekaterina Jalomo-Khayrova, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Ignacio A. Rivero and Marco A. Ramos

    Citation: BMC Biotechnology 2018 18:20

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  5. Content type: Research article

    Safe and effective hemostatic materials are important for reducing mortality resulting from excessive hemorrhage. In this work, new biomaterials with hemostatic effects were created by fusing the gene coding f...

    Authors: Shasha Yang, Sili Wei, Yun Mao, Hanxue Zheng, Juantao Feng, Jihong Cui, Xin Xie, Fulin Chen and Honmgmin Li

    Citation: BMC Biotechnology 2018 18:12

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  6. Content type: Research article

    The recently developed genetically encoded calcium indicator (GECI), called NTnC, has a novel design with reduced size due to utilization of the troponin C (TnC) as a Ca2+-binding moiety inserted into the mNeonGr...

    Authors: D. A. Doronin, N. V. Barykina, O. M. Subach, V. P. Sotskov, V. V. Plusnin, O. A. Ivleva, E. A. Isaakova, A. M. Varizhuk, G. E. Pozmogova, A. Y. Malyshev, I. V. Smirnov, K. D. Piatkevich, K. V. Anokhin, G. N. Enikolopov and F. V. Subach

    Citation: BMC Biotechnology 2018 18:10

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  7. Content type: Research article

    The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and...

    Authors: Anil S. Prajapati, Vishakha A. Pawar, Ketankumar J. Panchal, Ankit P. Sudhir, Bhaumik R. Dave, Darshan H. Patel and R. B. Subramanian

    Citation: BMC Biotechnology 2018 18:9

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  8. Content type: Research article

    Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products depending on the regioselectivity of the immobilized lipase biocatalyst

    Authors: Erick Abreu Silveira, Sonia Moreno-Perez, Alessandra Basso, Simona Serban, Rita Pestana Mamede, Paulo W. Tardioli, Cristiane Sanchez Farinas, Javier Rocha-Martin, Gloria Fernandez-Lorente and Jose M. Guisan

    Citation: BMC Biotechnology 2017 17:88

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  9. Content type: Research article

    Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO4], [Cl], [MeCO2]) were...

    Authors: Zhao Wang, John Gräsvik, Leif J. Jönsson and Sandra Winestrand

    Citation: BMC Biotechnology 2017 17:82

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  10. Content type: Research article

    The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus ...

    Authors: Andris Kazaks, I-Na Lu, Sophie Farinelle, Alex Ramirez, Vincenzo Crescente, Benjamin Blaha, Olotu Ogonah, Tarit Mukhopadhyay, Mapi Perez de Obanos, Alejandro Krimer, Inara Akopjana, Janis Bogans, Velta Ose, Anna Kirsteina, Tatjana Kazaka, Nicola J. Stonehouse…

    Citation: BMC Biotechnology 2017 17:79

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  11. Content type: Research article

    Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and antiba...

    Authors: Natalia Inés Almasia, María Paula Molinari, Guillermo Andrés Maroniche, Vanesa Nahirñak, María Pilar Barrios Barón, Oscar Alberto Taboga and Cecilia Vazquez Rovere

    Citation: BMC Biotechnology 2017 17:75

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  12. Content type: Research article

    Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed...

    Authors: Aldricho Alpha Pollardo, Hong-shik Lee, Dohoon Lee, Sangyong Kim and Jaehoon Kim

    Citation: BMC Biotechnology 2017 17:70

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  13. Content type: Research article

    Fibroblast growth factor 21 (FGF21) is an endocrine-acting hormone that has the potential to treat diabetic nephropathy. However, development of FGF21 into a therapeutic has been hindered due to its low intrin...

    Authors: Longwei Zhao, Huiyan Wang, Junjun Xie, Zilu Chen, Xiaokun Li and Jianlou Niu

    Citation: BMC Biotechnology 2017 17:58

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  14. Content type: Research article

    Fas ligand plays a key role in the human immune system as a major cell death inducing protein. The extracellular domain of human Fas ligand (hFasLECD) triggers apoptosis of malignant cells, and therefore is ex...

    Authors: Michiro Muraki and Kiyonori Hirota

    Citation: BMC Biotechnology 2017 17:56

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  15. Content type: Research article

    Effective and simple methods that lead to higher enzymatic efficiencies are highly sough. Here we proposed a foldon-triggered trimerization of the target enzymes with significantly improved catalytic performan...

    Authors: Xinzhe Wang, Huihua Ge, Dandan Zhang, Shuyu Wu and Guangya Zhang

    Citation: BMC Biotechnology 2017 17:57

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  16. Content type: Research Article

    Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are su...

    Authors: Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu and Rijun Zhang

    Citation: BMC Biotechnology 2017 17:55

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  17. Content type: Research article

    Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Authors: Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    Citation: BMC Biotechnology 2017 17:37

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  18. Content type: Research article

    Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k ...

    Authors: Qing Wang, Luyao Bao, Chenjun Jia, Mei Li, Jian-Jun Li and Xuefeng Lu

    Citation: BMC Biotechnology 2017 17:31

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Page 1 of 7

2016 Journal Metrics

  • Citation Impact
    2.415 - 2-year Impact Factor
    2.865 - 5-year Impact Factor
    0.882 - Source Normalized Impact per Paper (SNIP)
    0.883 - SCImago Journal Rank (SJR)

    Usage 
    694,556 Downloads
    1786.5 Usage Factor

    Social Media Impact
    99 Mentions

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