Plant biotechnology

Section edited by Neal C Stewart

This section will consider studies that focus on transgenic plants and their applications, including agriculture, bioproduction, detection, biosafety, phytoremediation and biofuels.

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  1. Methodology article

    A bioinformatics approach for identifying transgene insertion sites using whole genome sequencing data

    Genetically modified crops (GM crops) have been developed to improve the agricultural traits of modern crop cultivars. Safety assessments of GM crops are of paramount importance in research at developmental st...

    Doori Park, Su-Hyun Park, Yong Wook Ban, Youn Shic Kim, Kyoung-Cheul Park, Nam-Soo Kim, Ju-Kon Kim and Ik-Young Choi

    BMC Biotechnology 2017 17:67

    Published on: 15 August 2017

  2. Research article

    Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in crops

    Development of chimeric Cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes. Expression of novel C...

    Maniraj Rathinam, Shweta Singh, Debasis Pattanayak and Rohini Sreevathsa

    BMC Biotechnology 2017 17:64

    Published on: 2 August 2017

  3. Research article

    Somatic cell selection for chlorsulfuron-resistant mutants in potato: identification of point mutations in the acetohydroxyacid synthase gene

    Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in ...

    Philippa J. Barrell, Julie M. Latimer, Samantha J. Baldwin, Michelle L. Thompson, Jeanne M.E. Jacobs and Anthony J. Conner

    BMC Biotechnology 2017 17:49

    Published on: 6 June 2017

  4. Research article

    Pollen-mediated gene flow from transgenic to non-transgenic switchgrass (Panicum virgatum L.) in the field

    Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter...

    Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R. Johnson, Micaha Hanson, Jason N. Burris, Charles Kwit and C. Neal Stewart Jr

    BMC Biotechnology 2017 17:40

    Published on: 2 May 2017

  5. Methodology article

    Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

    Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have...

    Jun-Zheng Wu, Qin Liu, Xiao-Shan Geng, Kai-Mian Li, Li-Juan Luo and Jin-Ping Liu

    BMC Biotechnology 2017 17:29

    Published on: 14 March 2017

  6. Research article

    The role of community engagement in the adoption of new agricultural biotechnologies by farmers: the case of the Africa harvest tissue-culture banana in Kenya

    The tissue culture banana (TCB) is a biotechnological agricultural innovation that has been adopted widely in commercial banana production. In 2003, Africa Harvest Biotech Foundation International (AH) initiat...

    Sunita V. S. Bandewar, Florence Wambugu, Emma Richardson and James V. Lavery

    BMC Biotechnology 2017 17:28

    Published on: 13 March 2017

  7. Research article

    Transcriptomic response of wolf spider, Pardosa pseudoannulata, to transgenic rice expressing Bacillus thuringiensis Cry1Ab protein

    Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthet...

    Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song and Xuguo Zhou

    BMC Biotechnology 2017 17:7

    Published on: 18 January 2017

  8. Methodology article

    Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

    Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid trans...

    Kalpana Nanjareddy, Manoj-Kumar Arthikala, Lourdes Blanco, Elizabeth S. Arellano and Miguel Lara

    BMC Biotechnology 2016 16:53

    Published on: 24 June 2016

  9. Research article

    Diversity of ABC transporter genes across the plant kingdom and their potential utility in biotechnology

    The ATP-binding cassette (ABC) transporter gene superfamily is ubiquitous among extant organisms and prominently represented in plants. ABC transporters act to transport compounds across cellular membranes and...

    Thomas S. Lane, Caroline S. Rempe, Jack Davitt, Margaret E. Staton, Yanhui Peng, Douglas Edward Soltis, Michael Melkonian, Michael Deyholos, James H. Leebens-Mack, Mark Chase, Carl J. Rothfels, Dennis Stevenson, Sean W. Graham, Jun Yu, Tao Liu, J. Chris Pires…

    BMC Biotechnology 2016 16:47

    Published on: 31 May 2016

  10. Research article

    Novel strong promoter of antimicrobial peptides gene pro-SmAMP2 from chickweed (Stellaria media)

    In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the β-actin gene. This high level of the gene expression has attracted our atten...

    Roman A. Komakhin, Denis A. Vysotskii, Rahim R. Shukurov, Vera D. Voblikova, Vera V. Komakhina, Svetlana R. Strelnikova, Ekaterina M. Vetchinkina and Alexey V. Babakov

    BMC Biotechnology 2016 16:43

    Published on: 18 May 2016

  11. Research article

    Genomic variation and DNA repair associated with soybean transgenesis: a comparison to cultivars and mutagenized plants

    The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will p...

    Justin E. Anderson, Jean-Michel Michno, Thomas J. Y. Kono, Adrian O. Stec, Benjamin W. Campbell, Shaun J. Curtin and Robert M. Stupar

    BMC Biotechnology 2016 16:41

    Published on: 12 May 2016

  12. Research article

    Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50

    Despite the limited success after decades of intensive research and development efforts, vaccination still represents the most promising strategy to significantly reduce the disease burden in malaria endemic r...

    Veronique Beiss, Holger Spiegel, Alexander Boes, Matthias Scheuermayer, Andreas Reimann, Stefan Schillberg and Rainer Fischer

    BMC Biotechnology 2015 15:108

    Published on: 1 December 2015

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