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Plant biotechnology

This section will consider studies that focus on transgenic plants and their applications, including agriculture, bioproduction, detection, biosafety, phytoremediation and biofuels.

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  1. In order to improve the taste acceptability of certain nutritional oils, it has been decided in this study to introduce them in an emulsion whose surfactant is casein, then to carry out a lacto-fermentation, l...

    Authors: Soumaya El Bouchikhi, Philippe Pagès, Azeddine Ibrahimi and Yahya Bensouda

    Citation: BMC Biotechnology 2021 21:53

    Content type: Research article

    Published on:

  2. Phakopsora pachyrhizi is a biotrophic fungal pathogen responsible for the Asian soybean rust disease causing important yield losses in tropical and subtropical soybean-producing countries. P. pachyrhizi triggers ...

    Authors: L. Cabre, S. Peyrard, C. Sirven, L. Gilles, B. Pelissier, S. Ducerf and N. Poussereau

    Citation: BMC Biotechnology 2021 21:27

    Content type: Research article

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  3. Lyso-phosphatidylethanolamine (LPE) is a natural phospholipid that functions in the early stages of plant senescence. Plant innate immunity and early leaf senescence share molecular components. To reveal conserve...

    Authors: Ronny Völz, Ju-Young Park, William Harris, Sungkee Hwang and Yong-Hwan Lee

    Citation: BMC Biotechnology 2021 21:12

    Content type: Research article

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  4. Moringa stenopetala belongs to the flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The e...

    Authors: Alelegne Yeshamebel Adugna, Tileye Feyissa and Fikresilasie Samuel Tasew

    Citation: BMC Biotechnology 2020 20:60

    Content type: Research article

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  5. Development of precise genome editing strategies is a prerequisite for producing edited plants that can aid in the study of gene function and help understand the genetic traits in a cultivar. Citrus embryogeni...

    Authors: Manjul Dutt, Zhonglin Mou, Xudong Zhang, Sameena E. Tanwir and Jude W. Grosser

    Citation: BMC Biotechnology 2020 20:58

    Content type: Research article

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  6. Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosa...

    Authors: Amitchihoué Franck Sessou, Jane W. Kahia, Jerome Anani Houngue, Elijah Miinda Ateka, Colombe Dadjo and Corneille Ahanhanzo

    Citation: BMC Biotechnology 2020 20:51

    Content type: Research article

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  7. Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit devel...

    Authors: Kasturi Dasgupta, Sara Hotton, William Belknap, Yasra Syed, Christopher Dardick, Roger Thilmony and James G. Thomson

    Citation: BMC Biotechnology 2020 20:43

    Content type: Research article

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  8. Gene editing using the CRISPR/Cas9 system has become a routinely applied method in several plant species. The most convenient gene delivery system is Agrobacterium-mediated gene transfer with antibiotic selection...

    Authors: Zsófia Bánfalvi, Edina Csákvári, Vanda Villányi and Mihály Kondrák

    Citation: BMC Biotechnology 2020 20:25

    Content type: Research article

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  9. The coexistence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals has deleterious effects on environmental quality. Few reports have studied the mechanisms of plant inoculation with Piriformospora indic...

    Authors: Liang Li, Pengyue Zhu, Xiaoyang Wang and Zhenhua Zhang

    Citation: BMC Biotechnology 2020 20:20

    Content type: Research article

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  10. Infections caused by fungi are often refractory to conventional therapies and urgently require the development of novel options, such as immunotherapy. To produce therapeutic antibodies, a plant-based expressi...

    Authors: Marcello Catellani, Chiara Lico, Mauro Cerasi, Silvia Massa, Carla Bromuro, Antonella Torosantucci, Eugenio Benvenuto and Cristina Capodicasa

    Citation: BMC Biotechnology 2020 20:15

    Content type: Research article

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  11. As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in ...

    Authors: Jean-Michel Michno, Kamaldeep Virdi, Adrian O. Stec, Junqi Liu, Xiaobo Wang, Yer Xiong and Robert M. Stupar

    Citation: BMC Biotechnology 2020 20:10

    Content type: Research article

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  12. Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate...

    Authors: Yanqin Xu, Junjiang Zhou, Qingqing Liu, Kunpeng Li and Yin Zhou

    Citation: BMC Biotechnology 2020 20:4

    Content type: Research article

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  13. Clostridium perfringens, a gram-positive, anaerobic, rod-shaped bacterium, is the third leading cause of human foodborne bacterial disease and a cause of necrotic enteritis in poultry. It is controlled using anti...

    Authors: Rosemarie W. Hammond, Steven M. Swift, Juli A. Foster-Frey, Natalia Y. Kovalskaya and David M. Donovan

    Citation: BMC Biotechnology 2019 19:101

    Content type: Research article

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  14. The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensiv...

    Authors: Jie Dong, Xiangzhen Ding and Sheng Wang

    Citation: BMC Biotechnology 2019 19:86

    Content type: Research article

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  15. Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When p...

    Authors: Elinea O. Freitas, Bruno P. Melo, Isabela T. Lourenço-Tessutti, Fabrício B. M. Arraes, Regina M. Amorim, Maria E. Lisei-de-Sá, Julia A. Costa, Ana G. B. Leite, Muhammad Faheem, Márcio A. Ferreira, Carolina V. Morgante, Elizabeth P. B. Fontes and Maria F. Grossi-de-Sa

    Citation: BMC Biotechnology 2019 19:79

    Content type: Research article

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  16. Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assa...

    Authors: Aurélie Bak and Joanne B. Emerson

    Citation: BMC Biotechnology 2019 19:73

    Content type: Methodology article

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  17. The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity...

    Authors: Taj Arndell, Niharika Sharma, Peter Langridge, Ute Baumann, Nathan S. Watson-Haigh and Ryan Whitford

    Citation: BMC Biotechnology 2019 19:71

    Content type: Research article

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  18. Conventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the resear...

    Authors: Prassan Choudhary, Sudipta Das, Hillol Chakdar, Arjun Singh, Sanjay Kumar Goswami and Anil Kumar Saxena

    Citation: BMC Biotechnology 2019 19:69

    Content type: Methodology article

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  19. Cry8-like from Bacillus thuringiensis (Bt) encodes an insecticidal crystal (Cry) protein. Holotrichia parallela (Coleoptera: Scarabaeoidae), commonly known as the dark black chafer, is a troublesome pest of soybe...

    Authors: Di Qin, Xiao-Yi Liu, Cristina Miceli, Qi Zhang and Pi-wu Wang

    Citation: BMC Biotechnology 2019 19:66

    Content type: Research article

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  21. CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly cont...

    Authors: Bent Larsen Petersen, Svenning Rune Möller, Jozef Mravec, Bodil Jørgensen, Mikkel Christensen, Ying Liu, Hans H. Wandall, Eric Paul Bennett and Zhang Yang

    Citation: BMC Biotechnology 2019 19:36

    Content type: Research article

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  22. Increasing the content of oleic acid in peanut seeds is one of the major goals in peanut breeding due to consumer and industry benefits, such as anti-oxidation and long shelf-life. Homeologous ahFAD2A and ahFAD2B

    Authors: Mei Yuan, Jun Zhu, Limin Gong, Liangqiong He, Crystal Lee, Suoyi Han, Charles Chen and Guohao He

    Citation: BMC Biotechnology 2019 19:24

    Content type: Research article

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  23. Insect resistance in crops represents a main challenge for agriculture. Transgenic approaches based on proteins displaying insect resistance properties are widely used as efficient breeding strategies. To exte...

    Authors: Guilherme Souza Prado, Pingdwende Kader Aziz Bamogo, Joel Antônio Cordeiro de Abreu, François-Xavier Gillet, Vanessa Olinto dos Santos, Maria Cristina Mattar Silva, Jean-Paul Brizard, Marcelo Porto Bemquerer, Martine Bangratz, Christophe Brugidou, Drissa Sérémé, Maria Fatima Grossi-de-Sa and Séverine Lacombe

    Citation: BMC Biotechnology 2019 19:15

    Content type: Research article

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  24. Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system...

    Authors: Noor al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif and Piwu Wang

    Citation: BMC Biotechnology 2019 19:9

    Content type: Research article

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    The Correction to this article has been published in BMC Biotechnology 2020 20:42

  25. Genetic transformation of microalgae has been hampered by inefficient transgene expression, limiting the progress of microalgal biotechnology. Many vector tools and strategies have been developed in recent yea...

    Authors: Anongpat Suttangkakul, Anchalee Sirikhachornkit, Piyada Juntawong, Wilasinee Puangtame, Thitikorn Chomtong, Suchada Srifa, Sukhita Sathitnaitham, Wasawat Dumrongthawatchai, Kanidtha Jariyachawalid and Supachai Vuttipongchaikij

    Citation: BMC Biotechnology 2019 19:4

    Content type: Research article

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  26. Rift Valley fever virus (RVFV), the causative agent of Rift Valley fever, is an enveloped single-stranded negative-sense RNA virus in the genus Phlebovirus, family Bunyaviridae. The virus is spread by infected mo...

    Authors: Sandiswa Mbewana, Ann E. Meyers, Brandon Weber, Vuyokazi Mareledwane, Maryke L. Ferreira, Phelix A. O. Majiwa and Edward P. Rybicki

    Citation: BMC Biotechnology 2018 18:77

    Content type: Research article

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  27. Transgenic technology has become an important technique for crop genetic improvement. The application of well-characterized promoters is essential for developing a vector system for efficient genetic transform...

    Authors: Pingping Jiang, Ke Zhang, Zhaohua Ding, Qiuxia He, Wendi Li, Shuangfeng Zhu, Wen Cheng, Kewei Zhang and Kunpeng Li

    Citation: BMC Biotechnology 2018 18:59

    Content type: Research article

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  28. Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell...

    Authors: Guotian Li, Kyle C. Jones, Aymerick Eudes, Venkataramana R. Pidatala, Jian Sun, Feng Xu, Chengcheng Zhang, Tong Wei, Rashmi Jain, Devon Birdseye, Patrick E. Canlas, Edward E. K. Baidoo, Phat Q. Duong, Manoj K. Sharma, Seema Singh, Deling Ruan…

    Citation: BMC Biotechnology 2018 18:54

    Content type: Research article

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  29. Fibroblast growth factor 9 (FGF9) is a heparin-binding growth factor, secreted by both mesothelial and epithelial cells, which participates in hair follicle regeneration, wound healing, and bone development. A...

    Authors: Jingbo Cai, Ruicheng Wen, Wenqing Li, Xiuran Wang, Haishan Tian, Shanyong Yi, Linbo Zhang, Xiaokun Li, Chao Jiang and Haiyan Li

    Citation: BMC Biotechnology 2018 18:51

    Content type: Research Article

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  31. The advent of –omics technologies has enabled the resolution of fine molecular differences among individuals within a species. DNA sequence variations, such as single nucleotide polymorphisms or small deletion...

    Authors: Jean-Michel Michno and Robert M. Stupar

    Citation: BMC Biotechnology 2018 18:38

    Content type: Research article

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  32. RNA interference (RNAi) technology has been widely used to knockdown target genes via post-transcriptional silencing. In plants, RNAi is used as an effective tool with diverse applications being developed such...

    Authors: Po-Hao Wang, Greg Schulenberg, Shannon Whitlock, Andrew Worden, Ning Zhou, Stephen Novak and Wei Chen

    Citation: BMC Biotechnology 2018 18:3

    Content type: Methodology article

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  33. Genetically modified crops (GM crops) have been developed to improve the agricultural traits of modern crop cultivars. Safety assessments of GM crops are of paramount importance in research at developmental st...

    Authors: Doori Park, Su-Hyun Park, Yong Wook Ban, Youn Shic Kim, Kyoung-Cheul Park, Nam-Soo Kim, Ju-Kon Kim and Ik-Young Choi

    Citation: BMC Biotechnology 2017 17:67

    Content type: Methodology article

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  34. Development of chimeric Cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes. Expression of novel C...

    Authors: Maniraj Rathinam, Shweta Singh, Debasis Pattanayak and Rohini Sreevathsa

    Citation: BMC Biotechnology 2017 17:64

    Content type: Research article

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  35. Alpha amylase hydrolyzes α-bonds of polysaccharides such as starch and produces malto-oligosaccharides. Its starch saccharification applications make it an essential enzyme in the textile, food and brewing ind...

    Authors: Hong Zhu, L. Bruce Reynolds and Rima Menassa

    Citation: BMC Biotechnology 2017 17:53

    Content type: Research article

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  36. Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only...

    Authors: Yan-Jun Li, Shou-Hong Zhu, Xin-Yu Zhang, Yong-Chang Liu, Fei Xue, Lan-Jie Zhao and Jie Sun

    Citation: BMC Biotechnology 2017 17:50

    Content type: Research article

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  37. Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in ...

    Authors: Philippa J. Barrell, Julie M. Latimer, Samantha J. Baldwin, Michelle L. Thompson, Jeanne M.E. Jacobs and Anthony J. Conner

    Citation: BMC Biotechnology 2017 17:49

    Content type: Research article

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  39. Switchgrass is C4 perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter...

    Authors: Reginald Millwood, Madhugiri Nageswara-Rao, Rongjian Ye, Ellie Terry-Emert, Chelsea R. Johnson, Micaha Hanson, Jason N. Burris, Charles Kwit and C. Neal Stewart Jr

    Citation: BMC Biotechnology 2017 17:40

    Content type: Research article

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  41. Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have...

    Authors: Jun-Zheng Wu, Qin Liu, Xiao-Shan Geng, Kai-Mian Li, Li-Juan Luo and Jin-Ping Liu

    Citation: BMC Biotechnology 2017 17:29

    Content type: Methodology article

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  42. The tissue culture banana (TCB) is a biotechnological agricultural innovation that has been adopted widely in commercial banana production. In 2003, Africa Harvest Biotech Foundation International (AH) initiat...

    Authors: Sunita V. S. Bandewar, Florence Wambugu, Emma Richardson and James V. Lavery

    Citation: BMC Biotechnology 2017 17:28

    Content type: Research article

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  43. Xylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown.

    Authors: Yihua Zhan, Xiangyu Sun, Guozeng Rong, Chunxiao Hou, Yingying Huang, Dean Jiang and Xiaoyan Weng

    Citation: BMC Biotechnology 2017 17:26

    Content type: Research article

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  44. Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthet...

    Authors: Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song and Xuguo Zhou

    Citation: BMC Biotechnology 2017 17:7

    Content type: Research article

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  45. Abiotic stresses are serious threats to plant growth, productivity and result in crop loss worldwide, reducing average yields of most major crops. Although abiotic stresses might elicit different plant respons...

    Authors: Yuanyuan Bu, Bo Sun, Aimin Zhou, Xinxin Zhang, Testuo Takano and Shenkui Liu

    Citation: BMC Biotechnology 2016 16:69

    Content type: Research article

    Published on:

  46. CRISPR/Cas9 genome editing strategy has been applied to a variety of species and the tRNA-processing system has been used to compact multiple gRNAs into one synthetic gene for manipulating multiple genes in rice.

    Authors: Weiwei Qi, Tong Zhu, Zhongrui Tian, Chaobin Li, Wei Zhang and Rentao Song

    Citation: BMC Biotechnology 2016 16:58

    Content type: Methodology article

    Published on:

  47. Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid trans...

    Authors: Kalpana Nanjareddy, Manoj-Kumar Arthikala, Lourdes Blanco, Elizabeth S. Arellano and Miguel Lara

    Citation: BMC Biotechnology 2016 16:53

    Content type: Methodology article

    Published on:

  48. The ATP-binding cassette (ABC) transporter gene superfamily is ubiquitous among extant organisms and prominently represented in plants. ABC transporters act to transport compounds across cellular membranes and...

    Authors: Thomas S. Lane, Caroline S. Rempe, Jack Davitt, Margaret E. Staton, Yanhui Peng, Douglas Edward Soltis, Michael Melkonian, Michael Deyholos, James H. Leebens-Mack, Mark Chase, Carl J. Rothfels, Dennis Stevenson, Sean W. Graham, Jun Yu, Tao Liu, J. Chris Pires…

    Citation: BMC Biotechnology 2016 16:47

    Content type: Research article

    Published on:

  49. In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the β-actin gene. This high level of the gene expression has attracted our atten...

    Authors: Roman A. Komakhin, Denis A. Vysotskii, Rahim R. Shukurov, Vera D. Voblikova, Vera V. Komakhina, Svetlana R. Strelnikova, Ekaterina M. Vetchinkina and Alexey V. Babakov

    Citation: BMC Biotechnology 2016 16:43

    Content type: Research article

    Published on:

  50. The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will p...

    Authors: Justin E. Anderson, Jean-Michel Michno, Thomas J. Y. Kono, Adrian O. Stec, Benjamin W. Campbell, Shaun J. Curtin and Robert M. Stupar

    Citation: BMC Biotechnology 2016 16:41

    Content type: Research article

    Published on:

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