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Microbial biotechnology

This section will consider studies that focus on the genetic manipulation and synthetic/systems biology of bacteria, fungi, yeasts and algae in the context of bioproduction, fermentation, metabolic engineering, biocatalysis, bioremediation and biodegradation.

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  1. Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involve...

    Authors: Xin Chai, Xiuling Shang, Yu Zhang, Shuwen Liu, Yong Liang, Yun Zhang and Tingyi Wen

    Citation: BMC Biotechnology 2016 16:79

    Content type: Research article

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  2. Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of i...

    Authors: Hanna Amelina, Vera Moiseeva, Laura Catharine Collopy, Siân Rosanna Pearson, Christine Anne Armstrong and Kazunori Tomita

    Citation: BMC Biotechnology 2016 16:76

    Content type: Methodology article

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  3. Infectious clones are fundamental tools for the study of many viruses, allowing for efficient mutagenesis and reproducible production of genetically-defined strains. For the large dsDNA genomes of the herpesvi...

    Authors: Alexsia L. Richards, Patricia J. Sollars and Gregory A. Smith

    Citation: BMC Biotechnology 2016 16:64

    Content type: Methodology article

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  4. Diols are important monomers for the production of plastics and polyurethanes, which are widely used in our daily life. The medium-chain diols with one hydroxyl group at its subterminal end are able to confer ...

    Authors: Yanning Zheng, Lingling Li, Qiang Liu, Haibo Zhang, Yujin Cao, Mo Xian and Huizhou Liu

    Citation: BMC Biotechnology 2016 16:61

    Content type: Research article

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  5. Large efforts have focused on screening for genes involved in the virulence and pathogenicity of Verticillium dahliae, a destructive fungal pathogen of numerous plant species that is difficult to control once the...

    Authors: Latifur Rehman, Xiaofeng Su, Huiming Guo, Xiliang Qi and Hongmei Cheng

    Citation: BMC Biotechnology 2016 16:57

    Content type: Methodology article

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  6. Foot-and-mouth disease (FMD) is an acute, highly contagious disease that infects cloven-hoofed animals. Vaccination is an effective means of preventing and controlling FMD. Compared to conventional inactivated...

    Authors: Yan Xiao, Hong-Ying Chen, Yuzhou Wang, Bo Yin, Chaochao Lv, Xiaobing Mo, He Yan, Yajie Xuan, Yuxin Huang, Wenqiang Pang, Xiangdong Li, Y. Adam Yuan and Kegong Tian

    Citation: BMC Biotechnology 2016 16:56

    Content type: Research article

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  7. Succinate is a kind of industrially important C4 platform chemical for synthesis of high value added products. Due to the economical and environmental advantages, considerable efforts on metabolic engineering ...

    Authors: Ying Zhao, Chang-Song Wang, Fei-Fei Li, Zhen-Ning Liu and Guang-Rong Zhao

    Citation: BMC Biotechnology 2016 16:52

    Content type: Research article

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  8. Four antigenically distinct serotypes (1–4) of dengue viruses (DENVs) cause dengue disease. Antibodies to any one DENV serotype have the potential to predispose an individual to more severe disease upon infect...

    Authors: Ankur Poddar, Viswanathan Ramasamy, Rahul Shukla, Ravi Kant Rajpoot, Upasana Arora, Swatantra K. Jain, Sathyamangalam Swaminathan and Navin Khanna

    Citation: BMC Biotechnology 2016 16:50

    Content type: Research article

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  9. Microalgae have been recognized as a good food source of natural biologically active ingredients. Among them, the green microalga Euglena is a very promising food and nutritional supplements, providing high value...

    Authors: Min Zeng, Wenlong Hao, Yongdong Zou, Mengliang Shi, Yongguang Jiang, Peng Xiao, Anping Lei, Zhangli Hu, Weiwen Zhang, Liqing Zhao and Jiangxin Wang

    Citation: BMC Biotechnology 2016 16:49

    Content type: Research article

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  10. As the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production. Cassava residues (CR) are byproducts of the cassava starch industry ...

    Authors: Li Ji, Tianran Zheng, Pengxiang Zhao, Weiming Zhang and Jianxin Jiang

    Citation: BMC Biotechnology 2016 16:48

    Content type: Research article

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  11. Cell-penetrating peptides (CPPs) have been widely used as carriers to transport different molecules into living cells, whereas messenger RNAs (mRNAs) have been utilized as target molecules for the prevention a...

    Authors: Yanli Sun, Yanhua Sun, Ronglan Zhao and Kunshan Gao

    Citation: BMC Biotechnology 2016 16:46

    Content type: Research article

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  12. Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass...

    Authors: Dennis Lamers, Nick van Biezen, Dirk Martens, Linda Peters, Eric van de Zilver, Nicole Jacobs-van Dreumel, René H. Wijffels and Christien Lokman

    Citation: BMC Biotechnology 2016 16:45

    Content type: Research article

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  13. L-(+)-tartaric acid (L-TA) is an important organic acid, which is produced from the cream of tartar or stereospecific hydrolysis of the cis-epoxysuccinate. The former method is limited by the availability of raw ...

    Authors: Jianfeng Yuan, Mianbin Wu, Jianping Lin and Lirong Yang

    Citation: BMC Biotechnology 2016 16:42

    Content type: Research article

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  14. Social amoeba, Dictyostelium discoideum, is a well-established model organism for studying cellular physiology and developmental pattern formation. Its haploid genome facilitates functional analysis of genes by a...

    Authors: Asuka Mukai, Aya Ichiraku and Kazuki Horikawa

    Citation: BMC Biotechnology 2016 16:37

    Content type: Methodology article

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  15. Vanillin, a type of phenolic released during the pre-treatment of lignocellulosic materials, is toxic to microorganisms and therefore its presence inhibits the fermentation. The vanillin can be reduced to vani...

    Authors: Xinning Wang, Zhenzhen Liang, Jin Hou, Xiaoming Bao and Yu Shen

    Citation: BMC Biotechnology 2016 16:31

    Content type: Research article

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  16. Hydroxy fatty acids (HFAs) are valuable chemicals for a broad variety of applications. However, commercial production of HFAs has not been established so far due to the lack of low cost routes for their synthe...

    Authors: Yujin Cao, Tao Cheng, Guang Zhao, Wei Niu, Jiantao Guo, Mo Xian and Huizhou Liu

    Citation: BMC Biotechnology 2016 16:26

    Content type: Research article

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  17. Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here...

    Authors: Palanisamy Athiyaman Balakumaran, Jan Förster, Martin Zimmermann, Jayachandran Charumathi, Andreas Schmitz, Eik Czarnotta, Mathias Lehnen, Suresh Sudarsan, Birgitta E. Ebert, Lars Mathias Blank and Sankaranarayanan Meenakshisundaram

    Citation: BMC Biotechnology 2016 16:20

    Content type: Research article

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  18. A thermal tolerant stereo-complex poly-lactic acid (SC-PLA) can be made by mixing Poly-D-lactic acid (PDLA) and poly-L-lactic acid (PLLA) at a defined ratio. This environmentally friendly biodegradable polymer...

    Authors: Hongying Lu, Xiao Zhao, Yongze Wang, Xiaoren Ding, Jinhua Wang, Erin Garza, Ryan Manow, Andrew Iverson and Shengde Zhou

    Citation: BMC Biotechnology 2016 16:19

    Content type: Research article

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  19. Nitrilases, which hydrolyze nitriles in a one-step reaction into carboxylic acids and ammonia, gained increasing attention because of the abundance of nitrile compounds in nature and their use in fine chemical...

    Authors: Sarah A. Sonbol, Ari J. S. Ferreira and Rania Siam

    Citation: BMC Biotechnology 2016 16:14

    Content type: Research article

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  20. Phage-encoded serine integrases, such as ϕC31 integrase, are widely used for genome engineering but have not been optimized for use in Saccharomyces cerevisiae although this organism is a widely used organism in ...

    Authors: Zhengyao Xu and William R. A. Brown

    Citation: BMC Biotechnology 2016 16:13

    Content type: Research article

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  21. Dengue is a viral disease spread to humans by mosquitoes. Notably, there are four serotypes of dengue viruses (DENV) that places ~40 % of the global population at risk of infection. However, lack of a suitable...

    Authors: Neha Kaushik, Deepak Rohila, Upasana Arora, Rajendra Raut, Urpo Lamminmäki, Navin Khanna and Gaurav Batra

    Citation: BMC Biotechnology 2016 16:12

    Content type: Research article

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  22. Marine fungi are a diverse group of opportunistic and obligate organisms isolated from marine environments. These fungi are now often included in screens for novel metabolites, while less attention has been gi...

    Authors: Yanming Wang, Dorothee Barth, Anu Tamminen and Marilyn G. Wiebe

    Citation: BMC Biotechnology 2016 16:3

    Content type: Research article

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  23. The exploitation of the surface display system of food and commensal lactic acid bacteria (LAB) for bacterial, viral, or protozoan antigen delivery has received strong interest recently. The Generally Regarded...

    Authors: Jeevanathan Kalyanasundram, Suet Lin Chia, Adelene Ai-Lian Song, Abdul Rahim Raha, Howard A. Young and Khatijah Yusoff

    Citation: BMC Biotechnology 2015 15:113

    Content type: Research article

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  24. RNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA targeting the viral gene. In this study, we employed a combinatorial approac...

    Authors: Thitiporn Thammasorn, Pakkakul Sangsuriya, Watcharachai Meemetta, Saengchan Senapin, Sarocha Jitrakorn, Triwit Rattanarojpong and Vanvimon Saksmerprome

    Citation: BMC Biotechnology 2015 15:110

    Content type: Research article

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  25. Actinobacillus pleuropneumoniae, a Gram-negative bacterium, is the causative agent of porcine pleuropneumonia, a highly contagious and often fatal disease. Because current vaccines con...

    Authors: Xuehe Hu, Hao Yan, Ke Liu, Jiansheng Hu, Chao Qi, Jihong Yang, Yanli Liu, Jin Zhao and Jinlin Liu

    Citation: BMC Biotechnology 2015 15:106

    Content type: Research article

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  26. In this paper we describe a novel method to achieve high yield bacterial expression of a small protein domain with considerable therapeutic potential; Domain I of Beta-2-glycoprotein I (β2GPI). β2GPI is intrin...

    Authors: Thomas McDonnell, Charis Pericleous, Emmanuelle Laurine, Rita Tommasi, Acely Garza-Garcia, Ian Giles, Yiannis Ioannou and Anisur Rahman

    Citation: BMC Biotechnology 2015 15:104

    Content type: Methodology article

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  27. Recently, the presence of an unauthorized genetically modified (GM) Bacillus subtilis bacterium overproducing vitamin B2 in a feed additive was notified by the Rapid Alert System for Food and Feed (RASFF). This h...

    Authors: Elodie Barbau-piednoir, Sigrid C. J. De Keersmaecker, Maud Delvoye, Céline Gau, Patrick Philipp and Nancy H. Roosens

    Citation: BMC Biotechnology 2015 15:103

    Content type: Research article

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  28. Lipases are regularly used in biotechnology to catalyse the hydrolysis of triglycerides and the synthesis of esters. Microbial lipases in particular have been widely used in a variety of industrial application...

    Authors: Wenjuan Yang, Hai Cao, Li Xu, Houjin Zhang and Yunjun Yan

    Citation: BMC Biotechnology 2015 15:94

    Content type: Research article

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  29. Phytase is used as an animal feed additive that degrades phytic acid and reduces feeding costs and pollution caused by fecal excretion of phosphorus. Some phytases have been expressed in Pichia pastoris, among wh...

    Authors: Cheng Li, Ying Lin, Xueyun Zheng, Nuo Pang, Xihao Liao, Xiaoxiao Liu, Yuanyuan Huang and Shuli Liang

    Citation: BMC Biotechnology 2015 15:88

    Content type: Research article

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  30. Bacterial transglutaminases are increasingly required as industrial reagents for in vitro modification of proteins in different fields such as in food processing as well as for enzymatic site-specific covalent co...

    Authors: Barbara Salis, Gaia Spinetti, Silvia Scaramuzza, Michele Bossi, Gloria Saccani Jotti, Giancarlo Tonon, Davide Crobu and Rodolfo Schrepfer

    Citation: BMC Biotechnology 2015 15:84

    Content type: Research article

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  31. The ∆6-desaturase gene, encoding a key enzyme in the biosynthesis of polyunsaturated fatty acids, has potential in pharmaceutical and nutraceutical applications.

    Authors: Sukanya Jeennor, Pattsarun Cheawchanlertfa, Sarinya Suttiwattanakul, Sarocha Panchanawaporn, Chanikul Chutrakul and Kobkul Laoteng

    Citation: BMC Biotechnology 2015 15:82

    Content type: Research article

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  32. Heterologous expression of psychrophilic enzymes in E. coli is particularly challenging due to their intrinsic instability. The low stability is regarded as a consequence of adaptation that allow them to function...

    Authors: Gro Elin Kjæreng Bjerga and Adele Kim Williamson

    Citation: BMC Biotechnology 2015 15:78

    Content type: Research article

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  33. Fungal laccases are multicopper oxidases (MCOs) with high biotechnological potential due to their capability to oxidize a wide range of aromatic contaminants using oxygen from the air. Albeit the numerous lacc...

    Authors: Ledys S. Copete, Xiomara Chanagá, Jorge Barriuso, María F. López-Lucendo, María J. Martínez and Susana Camarero

    Citation: BMC Biotechnology 2015 15:74

    Content type: Research article

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  34. Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pat...

    Authors: Milda Norkiene, Jomante Stonyte, Danguole Ziogiene, Egle Mazeike, Kestutis Sasnauskas and Alma Gedvilaite

    Citation: BMC Biotechnology 2015 15:68

    Content type: Research article

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  35. The myxomycete Physarum polycephalum appears to have remarkable potential as a lipid source for biodiesel production. The present study evaluated the use of rice bran as a carbon source and determined the medium ...

    Authors: Hanh Tran, Steven Stephenson and Erik Pollock

    Citation: BMC Biotechnology 2015 15:67

    Content type: Research article

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  36. Achieving a sustainable society requires, among other things, the use of renewable feedstocks to replace chemicals obtained from petroleum-derived compounds. Crude glycerol synthesized inexpensively as a bypro...

    Authors: Ignacio Poblete-Castro, Danielle Binger, Rene Oehlert and Manfred Rohde

    Citation: BMC Biotechnology 2014 14:962

    Content type: Research article

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  37. A laboratory-scale two-chamber microbial fuel cell employing an aerated cathode with no catalyst was inoculated with mixed inoculum and acetate as the carbon source.

    Authors: Diana Sanchez-Herrera, Daniella Pacheco-Catalan, Ruby Valdez-Ojeda, Blondy Canto-Canche, Xochitl Dominguez-Benetton, Jorge Domínguez-Maldonado and Liliana Alzate-Gaviria

    Citation: BMC Biotechnology 2014 14:102

    Content type: Research article

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  38. Trichoderma reesei is known as a good producer of industrial proteins but has hitherto been less successful in the production of therapeutic proteins. In order to elucidate the bottlenecks of heterologous protein...

    Authors: Wesley Smith, Jussi Jäntti, Merja Oja and Markku Saloheimo

    Citation: BMC Biotechnology 2014 14:91

    Content type: Research article

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  39. The study on the second generation bio-fuel is a hot area of current research of renewable energy. Among series of key points in this area, the role of β-glucosidase in the degradation of intermediate gluco-ol...

    Authors: Yadan Li, Ning Liu, Hui Yang, Fei Zhao, Ye Yu, Yun Tian and Xiangyang Lu

    Citation: BMC Biotechnology 2014 14:85

    Content type: Research article

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  40. Recently developed methods for genome editing in bacteria take advantage of the introduction of double-strand breaks by I-SceI in a mutation cassette to select for cells in which homologous recombination has h...

    Authors: Juhan Kim, Anthony M Webb, Jamie P Kershner, Stephen Blaskowski and Shelley D Copley

    Citation: BMC Biotechnology 2014 14:84

    Content type: Methodology article

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  41. Methylated resveratrol analogs show similar biological activities that are comparable with those of the resveratrol. However, the methylated resveratrol analogs exhibit better bioavailability as they are more ...

    Authors: Sun-Young Kang, Jae Kyoung Lee, Oksik Choi, Cha Young Kim, Jae-Hyuk Jang, Bang Yeon Hwang and Young-Soo Hong

    Citation: BMC Biotechnology 2014 14:67

    Content type: Research article

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  42. Temperature tolerance is an important aspect for commercial scale outdoor cultivation of microalgae and cyanobacteria. While various genes are known to be related to Synechocystis sp. PCC6803's heat shock respons...

    Authors: Ulrich M Tillich, Nick Wolter, Philipp Franke, Ulf Dühring and Marcus Frohme

    Citation: BMC Biotechnology 2014 14:66

    Content type: Research article

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