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Genome technology and molecular diagnostics

This section will consider studies that focus on high-throughput approaches, such as sequencing, amplification and microarrays, as well as technologies for molecular diagnostics.This section will consider studies that focus on high-throughput approaches, such as sequencing, amplification and microarrays, as well as technologies for molecular diagnostics.

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  1. Content type: Research article

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Authors: Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    Citation: BMC Biotechnology 2017 17:6

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  2. Content type: Research article

    The isolation of unknown DNA sequences flanked by known sequences is an important task in the event-specific detection of GMOs. None of event-specific detection method was developed based on the junction seque...

    Authors: Hongwei Gao, Xiaofan Yu, Tingting Deng, Min Sun, Xizhi Xiao, Xin Huang, Ying Chen and Ronggui Li

    Citation: BMC Biotechnology 2016 16:74

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  3. Content type: Research article

    Transcription factors (TFs) are effectors of cell signaling pathways that regulate gene expression. TF networks are highly interconnected; one signal can lead to changes in many TF levels, and one TF level can...

    Authors: Betul Bilgin, Aritro Nath, Christina Chan and S. Patrick Walton

    Citation: BMC Biotechnology 2016 16:62

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  4. Content type: Methodology article

    Selected microRNAs (miRNAs) that are abnormally expressed in the serum of patients with lung cancer have recently been proposed as biomarkers of this disease. The measurement of circulating miRNAs, however, re...

    Authors: Paola Campomenosi, Elisabetta Gini, Douglas M. Noonan, Albino Poli, Paola D’Antona, Nicola Rotolo, Lorenzo Dominioni and Andrea Imperatori

    Citation: BMC Biotechnology 2016 16:60

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  5. Content type: Methodology article

    Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appro...

    Authors: P. Scott Pine, Sarah A. Munro, Jerod R. Parsons, Jennifer McDaniel, Anne Bergstrom Lucas, Jean Lozach, Timothy G. Myers, Qin Su, Sarah M. Jacobs-Helber and Marc Salit

    Citation: BMC Biotechnology 2016 16:54

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  6. Content type: Methodology article

    Research into gene expression enables scientists to decipher the complex regulatory networks that control fundamental biological processes. Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method...

    Authors: Nicky Boulter, Francia Garces Suarez, Stephen Schibeci, Trevor Sunderland, Ornella Tolhurst, Tegan Hunter, George Hodge, David Handelsman, Ulla Simanainen, Edward Hendriks and Karen Duggan

    Citation: BMC Biotechnology 2016 16:27

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  7. Content type: Research article

    The p53 tumor suppressor protein is mainly regulated by alterations in the half-life of the protein, resulting in significant differences in p53 protein levels in cells. The major regulator of this process is ...

    Authors: Ping Zhang, Anne Sophie Kratz, Mohammed Salama, Seham Elabd, Thorsten Heinrich, Joachim Wittbrodt, Christine Blattner and Gary Davidson

    Citation: BMC Biotechnology 2015 15:92

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  8. Content type: Research article

    Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses.

    Authors: Shunli Yang, Shuanghui Yin, Youjun Shang, Di Wang, Weimin Ma, Jijun He, Jianhong Guo, Jianping Cai and Xiangtao Liu

    Citation: BMC Biotechnology 2015 15:83

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  9. Content type: Methodology article

    With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel...

    Authors: Harshita Sharma, Yasunori Kinoshita, Seiichi Fujiu, Shota Nomura, Mizuho Sawada, Shamim Ahmed, Masaki Shibuya, Kosaku Shirai, Syota Takamatsu, Tsuyoshi Watanabe, Hitoshi Yamazaki, Ryohei Kamiyama, Tetsuya Kobayashi, Hidenao Arai, Miho Suzuki, Naoto Nemoto…

    Citation: BMC Biotechnology 2014 14:78

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  10. Content type: Methodology article

    Neonatal dried blood spots (DBS) represent an inexpensive method for long-term biobanking worldwide and are considered gold mines for research for several human diseases, including those of metabolic, infectio...

    Authors: Akram Ghantous, Richard Saffery, Marie-Pierre Cros, Anne-Louise Ponsonby, Steven Hirschfeld, Carol Kasten, Terence Dwyer, Zdenko Herceg and Hector Hernandez-Vargas

    Citation: BMC Biotechnology 2014 14:60

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  11. Content type: Research article

    Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicat...

    Authors: Wenjing Pan, Miranda Byrne-Steele, Chunlin Wang, Stanley Lu, Scott Clemmons, Robert J Zahorchak and Jian Han

    Citation: BMC Biotechnology 2014 14:10

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  12. Content type: Methodology article

    Methicillin-resistant Staphylococcus aureus (MRSA) has become one of the most prevalent pathogens responsible for nosocomial infections throughout the world. As clinical MRSA diagnosis is concerned, current diagn...

    Authors: Jianyu Su, Xiaochen Liu, Hemiao Cui, Yanyan Li, Dingqiang Chen, Yanmei Li and Guangchao Yu

    Citation: BMC Biotechnology 2014 14:8

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  13. Content type: Methodology article

    The Nextera protocol, which utilises a transposome based approach to create libraries for Illumina sequencing, requires pure DNA template, an accurate assessment of input concentration and a column clean-up th...

    Authors: Sarah Lamble, Elizabeth Batty, Moustafa Attar, David Buck, Rory Bowden, Gerton Lunter, Derrick Crook, Bassam El-Fahmawi and Paolo Piazza

    Citation: BMC Biotechnology 2013 13:104

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  14. Content type: Research article

    The starting material for RNA sequencing (RNA-seq) studies is usually total RNA or polyA+ RNA. Both forms of RNA represent heterogeneous pools of RNA molecules at different levels of maturation and processing....

    Authors: Ammar Zaghlool, Adam Ameur, Linnea Nyberg, Jonatan Halvardson, Manfred Grabherr, Lucia Cavelier and Lars Feuk

    Citation: BMC Biotechnology 2013 13:99

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  15. Content type: Research article

    Gene transcripts specifically expressed in a particular cell type (cell-type specific gene markers) are useful for its detection and isolation from a tissue or other cell mixtures. However, finding informative...

    Authors: Miguel A Andrade-Navarro, Femina Kanji, Carmen G Palii, Marjorie Brand, Harold Atkins and Carol Perez-Iratxeta

    Citation: BMC Biotechnology 2013 13:80

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  16. Content type: Research article

    Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studi...

    Authors: JiHoon E Joo, Ee Ming Wong, Laura Baglietto, Chol-Hee Jung, Helen Tsimiklis, Daniel J Park, Nicholas C Wong, Dallas R English, John L Hopper, Gianluca Severi, Graham G Giles and Melissa C Southey

    Citation: BMC Biotechnology 2013 13:23

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