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Genome technology and molecular diagnostics

This section will consider studies that focus on high-throughput approaches, such as sequencing, amplification and microarrays, as well as technologies for molecular diagnostics.This section will consider studies that focus on high-throughput approaches, such as sequencing, amplification and microarrays, as well as technologies for molecular diagnostics.

Page 1 of 2
  1. Content type: Methodology article

    With the rapid development of sequencing technologies, increasing amount of genomic information has been accumulated. To clone genes for further functional studies in large scale, a cheap, fast and efficient c...

    Authors: Qin Liu, Hui-Jie Dang, Yuan-Hang Wu, Min Li, Yin-Hua Chen, Xiao-Lei Niu, Kai-Mian Li and Li-Juan Luo

    Citation: BMC Biotechnology 2018 18:44

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  2. Content type: Research article

    Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences of interest, including their transgene flanking regions and the unnatural assoc...

    Authors: Marie-Alice Fraiture, Julie Vandamme, Philippe Herman and Nancy H. C. Roosens

    Citation: BMC Biotechnology 2018 18:40

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  3. Content type: Methodology article

    Custom synthesized DNA is in high demand for synthetic biology applications. However, current technologies to produce these sequences using assembly from DNA oligonucleotides are costly and labor-intensive. Th...

    Authors: Yuliya Khilko, Philip D. Weyman, John I. Glass, Mark D. Adams, Melanie A. McNeil and Peter B. Griffin

    Citation: BMC Biotechnology 2018 18:37

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  4. Content type: Methodology article

    We demonstrate the feasibility of creating a pair of reference samples to be used as surrogates for clinical samples measured in either a research or clinical laboratory setting. The reference sample paradigm ...

    Authors: P. Scott Pine, Steven P. Lund, Sanford A. Stass, Debra Kukuruga, Feng Jiang, Lynn Sorbara, Sudhir Srivastava and Marc Salit

    Citation: BMC Biotechnology 2018 18:17

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  5. Content type: Research article

    microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, ...

    Authors: Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson and Peter J. Leedman

    Citation: BMC Biotechnology 2018 18:16

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  6. Content type: Methodology article

    Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioi...

    Authors: Christoph Geisler

    Citation: BMC Biotechnology 2018 18:8

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  7. Content type: Methodology article

    Detection of DNA methylome at single-base resolution is a significant challenge but promises to shed considerable light on human disease etiology. Current technologies could not detect DNA methylation genome-w...

    Authors: Zhen Jia, Yueyi Shi, Lei Zhang, Yipeng Ren, Tong Wang, Lejun Xing, Baorong Zhang, Guolan Gao and Rongfa Bu

    Citation: BMC Biotechnology 2018 18:7

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  8. Content type: Methodology article

    Circulating microRNAs are undergoing exploratory use as safety biomarkers in drug development. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is one common approach used to quantitate l...

    Authors: Russell D. Wolfinger, Sudheer Beedanagari, Eric Boitier, Tao Chen, Philippe Couttet, Heidrun Ellinger-Ziegelbauer, Gregory Guillemain, Claire Mariet, Peter Mouritzen, Raegan O’Lone, P. Scott Pine, Tatiana Sharapova, Jian Yan, Peter S. Yuen and Karol L. Thompson

    Citation: BMC Biotechnology 2018 18:6

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  9. Content type: Research article

    Massively parallel genetic sequencing allows rapid testing of known intellectual disability (ID) genes. However, the discovery of novel syndromic ID genes requires molecular confirmation in at least a second o...

    Authors: Tracy Dudding-Byth, Anne Baxter, Elizabeth G. Holliday, Anna Hackett, Sheridan O’Donnell, Susan M. White, John Attia, Han Brunner, Bert de Vries, David Koolen, Tjitske Kleefstra, Seshika Ratwatte, Carlos Riveros, Steve Brain and Brian C. Lovell

    Citation: BMC Biotechnology 2017 17:90

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  10. Content type: Research article

    Next-generation sequencing (NGS) has transformed genomic research by reducing turnaround time and cost. However, no major breakthrough has been made in the upstream library preparation methods until the transp...

    Authors: Amirali Kia, Christian Gloeckner, Trina Osothprarop, Niall Gormley, Erin Bomati, Michelle Stephenson, Igor Goryshin and Molly Min He

    Citation: BMC Biotechnology 2017 17:6

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  11. Content type: Research article

    The isolation of unknown DNA sequences flanked by known sequences is an important task in the event-specific detection of GMOs. None of event-specific detection method was developed based on the junction seque...

    Authors: Hongwei Gao, Xiaofan Yu, Tingting Deng, Min Sun, Xizhi Xiao, Xin Huang, Ying Chen and Ronggui Li

    Citation: BMC Biotechnology 2016 16:74

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  12. Content type: Research article

    Transcription factors (TFs) are effectors of cell signaling pathways that regulate gene expression. TF networks are highly interconnected; one signal can lead to changes in many TF levels, and one TF level can...

    Authors: Betul Bilgin, Aritro Nath, Christina Chan and S. Patrick Walton

    Citation: BMC Biotechnology 2016 16:62

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  13. Content type: Methodology article

    Selected microRNAs (miRNAs) that are abnormally expressed in the serum of patients with lung cancer have recently been proposed as biomarkers of this disease. The measurement of circulating miRNAs, however, re...

    Authors: Paola Campomenosi, Elisabetta Gini, Douglas M. Noonan, Albino Poli, Paola D’Antona, Nicola Rotolo, Lorenzo Dominioni and Andrea Imperatori

    Citation: BMC Biotechnology 2016 16:60

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  14. Content type: Methodology article

    Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appro...

    Authors: P. Scott Pine, Sarah A. Munro, Jerod R. Parsons, Jennifer McDaniel, Anne Bergstrom Lucas, Jean Lozach, Timothy G. Myers, Qin Su, Sarah M. Jacobs-Helber and Marc Salit

    Citation: BMC Biotechnology 2016 16:54

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  15. Content type: Methodology article

    Research into gene expression enables scientists to decipher the complex regulatory networks that control fundamental biological processes. Quantitative real-time PCR (qPCR) is a powerful and ubiquitous method...

    Authors: Nicky Boulter, Francia Garces Suarez, Stephen Schibeci, Trevor Sunderland, Ornella Tolhurst, Tegan Hunter, George Hodge, David Handelsman, Ulla Simanainen, Edward Hendriks and Karen Duggan

    Citation: BMC Biotechnology 2016 16:27

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  16. Content type: Research article

    The p53 tumor suppressor protein is mainly regulated by alterations in the half-life of the protein, resulting in significant differences in p53 protein levels in cells. The major regulator of this process is ...

    Authors: Ping Zhang, Anne Sophie Kratz, Mohammed Salama, Seham Elabd, Thorsten Heinrich, Joachim Wittbrodt, Christine Blattner and Gary Davidson

    Citation: BMC Biotechnology 2015 15:92

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  17. Content type: Research article

    Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses.

    Authors: Shunli Yang, Shuanghui Yin, Youjun Shang, Di Wang, Weimin Ma, Jijun He, Jianhong Guo, Jianping Cai and Xiangtao Liu

    Citation: BMC Biotechnology 2015 15:83

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  18. Content type: Methodology article

    With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel...

    Authors: Harshita Sharma, Yasunori Kinoshita, Seiichi Fujiu, Shota Nomura, Mizuho Sawada, Shamim Ahmed, Masaki Shibuya, Kosaku Shirai, Syota Takamatsu, Tsuyoshi Watanabe, Hitoshi Yamazaki, Ryohei Kamiyama, Tetsuya Kobayashi, Hidenao Arai, Miho Suzuki, Naoto Nemoto…

    Citation: BMC Biotechnology 2014 14:78

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  19. Content type: Methodology article

    Neonatal dried blood spots (DBS) represent an inexpensive method for long-term biobanking worldwide and are considered gold mines for research for several human diseases, including those of metabolic, infectio...

    Authors: Akram Ghantous, Richard Saffery, Marie-Pierre Cros, Anne-Louise Ponsonby, Steven Hirschfeld, Carol Kasten, Terence Dwyer, Zdenko Herceg and Hector Hernandez-Vargas

    Citation: BMC Biotechnology 2014 14:60

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2016 Journal Metrics

  • Citation Impact
    2.415 - 2-year Impact Factor
    2.865 - 5-year Impact Factor
    0.882 - Source Normalized Impact per Paper (SNIP)
    0.883 - SCImago Journal Rank (SJR)

    Usage 
    694,556 Downloads
    1786.5 Usage Factor

    Social Media Impact
    99 Mentions

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