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Genetic engineering in animals and animal cell systems

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  1. Candidate genes for color pattern formation in butterfly wings have been known based on gene expression patterns since the 1990s, but their functions remain elusive due to a lack of a functional assay. Several...

    Authors: Bidur Dhungel, Yoshikazu Ohno, Rie Matayoshi and Joji M Otaki

    Citation: BMC Biotechnology 2013 13:27

    Content type: Research article

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  2. Currently, the step-wise integration of tet-dependent transactivator and tet-responsive expression unit is considered to be the most promising tool to achieve stable tet-controlled gene expression in cell popu...

    Authors: Niels Heinz, Katharina Hennig and Rainer Loew

    Citation: BMC Biotechnology 2013 13:5

    Content type: Methodology article

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  3. Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based ge...

    Authors: Nisha Hirani, Marcel Westenberg, Minaxi S Gami, Paul Davis, Ian A Hope and Colin T Dolphin

    Citation: BMC Biotechnology 2013 13:1

    Content type: Methodology article

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  4. Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for biomedical research. However, the efficiency of this approach is cruciall...

    Authors: Anne Richter, Mayuko Kurome, Barbara Kessler, Valeri Zakhartchenko, Nikolai Klymiuk, Hiroshi Nagashima, Eckhard Wolf and Annegret Wuensch

    Citation: BMC Biotechnology 2012 12:84

    Content type: Research article

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  5. There are few studies that have examined the potential of RNA inference (RNAi) to increase protein production in the baculovirus expression vector system (BEVS). Spodoptera frugiperda (fall armyworm) (Sf)-caspase...

    Authors: Yiu-Kay Lai, John T-A Hsu, Chih-Chieh Chu, Teng-Yuan Chang, Kao-Lu Pan and Chih-Chien Lin

    Citation: BMC Biotechnology 2012 12:83

    Content type: Research article

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  6. Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction met...

    Authors: Isabelle Houbracken, Luc Baeyens, Philippe Ravassard, Harry Heimberg and Luc Bouwens

    Citation: BMC Biotechnology 2012 12:74

    Content type: Research article

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  7. Gene targeting is a powerful method that can be used for examining the functions of genes. Traditionally, the construction of knockout (KO) vectors requires an amplification step to obtain two homologous, larg...

    Authors: Yi Liu, Shangze Li, Huihui Zhang, Zurong Wan, Xiaodong Zhang and Runlei Du

    Citation: BMC Biotechnology 2012 12:71

    Content type: Methodology article

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  8. Virus rescue from transfected cells is an extremely useful technique that allows defined viral clones to be engineered for the purpose of rational vaccine design or fundamental reverse genetics studies. Howeve...

    Authors: Vincent Bourret, Jon Lyall, Mariette F Ducatez, Jean-Luc Guérin and Laurence Tiley

    Citation: BMC Biotechnology 2012 12:69

    Content type: Methodology article

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  9. Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their...

    Authors: Asmita Patel, Anisleidys Muñoz, Katherine Halvorsen and Priyamvada Rai

    Citation: BMC Biotechnology 2012 12:3

    Content type: Methodology article

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  10. Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silence...

    Authors: Eriko Kage-Nakadai, Hiroyuki Kobuna, Osamu Funatsu, Muneyoshi Otori, Keiko Gengyo-Ando, Sawako Yoshina, Sayaka Hori and Shohei Mitani

    Citation: BMC Biotechnology 2012 12:1

    Content type: Methodology article

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  11. Physical mapping of transgenic insertions by Fluorescence in situ Hybridization (FISH) is a reliable and cost-effective technique. Chromosomal assignment is commonly achieved either by concurrent G-banding or ...

    Authors: Mohammed Yusuf, David LV Bauer, Daniel M Lipinski, Robert E MacLaren, Richard Wade-Martins, Kalim U Mir and Emanuela V Volpi

    Citation: BMC Biotechnology 2011 11:121

    Content type: Methodology article

    Published on:

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