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Genetic engineering in animals and animal cell systems

  1. Content type: Methodology article

    The CRISPR/Cas9 system has been widely used to generate gene knockout/knockin models by inducing frameshift mutants in cell lines and organisms. Several recent studies have reported that such mutants can lead ...

    Authors: Dafeng Chen, Ji-Xin Tang, Beibei Li, Li Hou, Xianhui Wang and Le Kang

    Citation: BMC Biotechnology 2018 18:60

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  2. Content type: Review

    Genome editing technologies offers new opportunities for tackling diseases such as acute lymphoblastic leukemia (ALL) that have been beyond the reach of previous therapies.

    Authors: Adrián Montaño, Maribel Forero-Castro, Jesús-María Hernández-Rivas, Ignacio García-Tuñón and Rocío Benito

    Citation: BMC Biotechnology 2018 18:45

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  3. Content type: Research article

    Recent progress in development of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal...

    Authors: Tomoe Kobayashi, Masumi Namba, Takayuki Koyano, Masaki Fukushima, Masahiro Sato, Masato Ohtsuka and Makoto Matsuyama

    Citation: BMC Biotechnology 2018 18:19

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  4. Content type: Methodology article

    Effective gene-delivery systems for primary human T cell engineering are useful tools for both basic research and clinical immunotherapy applications. Pseudovirus-based systems and electro-transfection are the...

    Authors: Zhang Zhang, Shunfang Qiu, Xiaopeng Zhang and Wei Chen

    Citation: BMC Biotechnology 2018 18:4

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  5. Content type: Research article

    The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) fo...

    Authors: Gerlinde Doenz, Sebastian Dorn, Narges Aghaallaei, Baubak Bajoghli, Elisabeth Riegel, Michaela Aigner, Holger Bock, Birgit Werner, Thomas Lindhorst and Thomas Czerny

    Citation: BMC Biotechnology 2018 18:1

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  6. Content type: Research article

    Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits a highly restricted tropism for cells of the monocyte-macrophage lineage, utilizing porcine CD163 (pCD163) as an indispensable cellular rece...

    Authors: Liangliang Li, Chunyan Wu, Gaopeng Hou, Biyun Xue, Sha Xie, Qin Zhao, Yuchen Nan, Gaiping Zhang and En-Min Zhou

    Citation: BMC Biotechnology 2017 17:77

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  7. Content type: Research article

    Pardosa pseudoannulata is a prevailing spider species, and has been regarded as an important bio-control agent of insect pests in farmland of China. However, the available genomic and ...

    Authors: Rong Li, Zhenzhen Yan, Juan Wang, Qisheng Song and Zhi Wang

    Citation: BMC Biotechnology 2017 17:73

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  9. Content type: Research article

    As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cel...

    Authors: Fatemeh Nematpour, Fereidoun Mahboudi, Behrouz Vaziri, Vahid Khalaj, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat and Fatemeh Davami

    Citation: BMC Biotechnology 2017 17:18

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  10. Content type: Research article

    Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter d...

    Authors: Steven C. L. Ho, Esther Y. C. Koh, Benjamin P. C. Soo, Mariati, Sheng-Hao Chao and Yuansheng Yang

    Citation: BMC Biotechnology 2016 16:71

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  11. Content type: Research article

    Diabetes and its concurrent complications impact a significant proportion of the population of the US and create a large financial burden on the American health care system. FDA-approved maggot debridement the...

    Authors: Rebecca J. Linger, Esther J. Belikoff, Ying Yan, Fang Li, Holly A. Wantuch, Helen L. Fitzsimons and Maxwell J. Scott

    Citation: BMC Biotechnology 2016 16:30

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  12. Content type: Research

    Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. However, the low natural abundance of IGF-I limits it...

    Authors: Shujie Liu, Yongming Li, Bo Deng and Ziwei Xu

    Citation: BMC Biotechnology 2016 16:25

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  13. Content type: Research article

    The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requi...

    Authors: C. Liesche, L. Venkatraman, S. Aschenbrenner, S. Grosse, D. Grimm, R. Eils and J. Beaudouin

    Citation: BMC Biotechnology 2016 16:17

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  14. Content type: Methodology article

    The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the...

    Authors: Van Trung Chu, Timm Weber, Robin Graf, Thomas Sommermann, Kerstin Petsch, Ulrike Sack, Pavel Volchkov, Klaus Rajewsky and Ralf Kühn

    Citation: BMC Biotechnology 2016 16:4

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  15. Content type: Research article

    Rational design of AAV capsids is a simple method for enhancing AAV transduction efficiency. AAV-DJ is a highly recombinogenic hybrid vector created from DNA shuffling of eight AAV serotypes, which mediates ef...

    Authors: Yingying Mao, Xuejun Wang, Renhe Yan, Wei Hu, Andrew Li, Shengqi Wang and Hongwei Li

    Citation: BMC Biotechnology 2016 16:1

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  16. Content type: Methodology article

    The demethylation potential of environmental pollutants is possibly an innate part of their comprehensive health risk. This paper develops a novel method called TDQ to quantify the demethylation epigenetic tox...

    Authors: Yan Qian, Xiaoli Wang, Zhanlu Lv, Chen Guo, Mei Han, Jiabing Wu, Yongjian Yang, Yishu Yang, Yan Jiang, Yongjie Wei, Jing Nie, Bao Liang, Jinliang Zhang and Xianliang Wang

    Citation: BMC Biotechnology 2015 15:107

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  17. Content type: Methodology article

    The cosmopolitan microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have its complete genome sequenced, an unpre...

    Authors: Chizue Hiruta, Yukiko Ogino, Tetsushi Sakuma, Kenji Toyota, Shinichi Miyagawa, Takashi Yamamoto and Taisen Iguchi

    Citation: BMC Biotechnology 2014 14:95

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  18. Content type: Research article

    Follistatin (FST) has been shown to bind to some TGF-β family members and can function as a potent myostatin (MSTN) antagonist. Recent studies have revealed that over-expression of FST by adeno-associated viru...

    Authors: Mahmood Nazari, Fatemeh Salabi, Li Zhang, Fuping Zhao, Caihong Wei and Lixin Du

    Citation: BMC Biotechnology 2014 14:87

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  19. Content type: Methodology article

    Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, ...

    Authors: Rajiv Kumar Yadav, Florian Barbi, Antoine Ziller, Patricia Luis, Roland Marmeisse, M Sudhakara Reddy and Laurence Fraissinet-Tachet

    Citation: BMC Biotechnology 2014 14:80

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  20. Content type: Research article

    During development of recombinant monoclonal antibodies in Chinese hamster ovary (CHO) cells, C-terminal amidated species are observed. C-terminal amidation is catalysed by peptidylglycine α-amidating monooxyg...

    Authors: Mihaela Škulj, Dejan Pezdirec, Dominik Gaser, Marko Kreft and Robert Zorec

    Citation: BMC Biotechnology 2014 14:76

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  22. Content type: Methodology article

    Microinjection of clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-related RNA and DNA into fertilized eggs is a novel approach for creating gene-modified m...

    Authors: Takayuki Sakurai, Satoshi Watanabe, Akiko Kamiyoshi, Masahiro Sato and Takayuki Shindo

    Citation: BMC Biotechnology 2014 14:69

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  23. Content type: Methodology article

    Establishing highly productive clonal cell lines with constant productivity over 2–3 months of continuous culture remains a tedious task requiring the screening of tens of thousands of clonal colonies. In addi...

    Authors: Nadezhda A Orlova, Sergey V Kovnir, Julia A Hodak, Ivan I Vorobiev, Alexandre G Gabibov and Konstantin G Skryabin

    Citation: BMC Biotechnology 2014 14:56

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  25. Content type: Research article

    High recombinant protein productivity in mammalian cell lines is often associated with phenotypic changes in protein content, energy metabolism, and cell growth, but the key determinants that regulate producti...

    Authors: Raihana Edros, Susan McDonnell and Mohamed Al-Rubeai

    Citation: BMC Biotechnology 2014 14:15

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  26. Content type: Methodology article

    The ubiquitous, freshwater microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have a well annotated, reference g...

    Authors: Chizue Hiruta, Kenji Toyota, Hitoshi Miyakawa, Yukiko Ogino, Shinichi Miyagawa, Norihisa Tatarazako, Joseph R Shaw and Taisen Iguchi

    Citation: BMC Biotechnology 2013 13:96

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  27. Content type: Research article

    Phage-encoded serine integrases, such as φC31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in un...

    Authors: Zhengyao Xu, Louise Thomas, Ben Davies, Ronald Chalmers, Maggie Smith and William Brown

    Citation: BMC Biotechnology 2013 13:87

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  28. Content type: Research article

    Up to now, the different uptake pathways and the subsequent intracellular trafficking of plasmid DNA have been largely explored. By contrast, the mode of internalization and the intracellular routing of an exo...

    Authors: Solenne Bire, David Gosset, Gwenhael Jégot, Patrick Midoux, Chantal Pichon and Florence Rouleux-Bonnin

    Citation: BMC Biotechnology 2013 13:75

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  29. Content type: Methodology article

    Stable expression of transgenes is an important technique to analyze gene function. Various drug resistance genes, such as neo, pac, hph, zeo, bsd, and hisD, have been equally used as selection markers to isolate...

    Authors: Yuhki Nakatake, Setsuko Fujii, Shinji Masui, Toshimi Sugimoto, Satomi Torikai-Nishikawa, Kenjiro Adachi and Hitoshi Niwa

    Citation: BMC Biotechnology 2013 13:64

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  30. Content type: Research article

    Diffusion of small molecules into fish embryos is essential for many experimental procedures in developmental biology and toxicology. Since we observed a weak uptake of lithium into medaka eggs we started a de...

    Authors: Gerlinde Jung, Markus Hug, Christian Halter, Andrea Friesenhengst, Johann Walzer and Thomas Czerny

    Citation: BMC Biotechnology 2013 13:53

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  31. Content type: Research article

    Somatic cell nuclear transfer (SCNT) using genetically engineered donor cells is currently the most widely used strategy to generate tailored pig models for biomedical research. Although this approach facilita...

    Authors: Mayuko Kurome, Ludwig Geistlinger, Barbara Kessler, Valeri Zakhartchenko, Nikolai Klymiuk, Annegret Wuensch, Anne Richter, Andrea Baehr, Katrin Kraehe, Katinka Burkhardt, Krzysztof Flisikowski, Tatiana Flisikowska, Claudia Merkl, Martina Landmann, Marina Durkovic, Alexander Tschukes…

    Citation: BMC Biotechnology 2013 13:43

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  33. Content type: Research article

    Candidate genes for color pattern formation in butterfly wings have been known based on gene expression patterns since the 1990s, but their functions remain elusive due to a lack of a functional assay. Several...

    Authors: Bidur Dhungel, Yoshikazu Ohno, Rie Matayoshi and Joji M Otaki

    Citation: BMC Biotechnology 2013 13:27

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  34. Content type: Methodology article

    Currently, the step-wise integration of tet-dependent transactivator and tet-responsive expression unit is considered to be the most promising tool to achieve stable tet-controlled gene expression in cell popu...

    Authors: Niels Heinz, Katharina Hennig and Rainer Loew

    Citation: BMC Biotechnology 2013 13:5

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  35. Content type: Methodology article

    Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based ge...

    Authors: Nisha Hirani, Marcel Westenberg, Minaxi S Gami, Paul Davis, Ian A Hope and Colin T Dolphin

    Citation: BMC Biotechnology 2013 13:1

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  36. Content type: Research article

    Somatic cell nuclear transfer (SCNT) is currently the most efficient and precise method to generate genetically tailored pig models for biomedical research. However, the efficiency of this approach is cruciall...

    Authors: Anne Richter, Mayuko Kurome, Barbara Kessler, Valeri Zakhartchenko, Nikolai Klymiuk, Hiroshi Nagashima, Eckhard Wolf and Annegret Wuensch

    Citation: BMC Biotechnology 2012 12:84

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  37. Content type: Research article

    There are few studies that have examined the potential of RNA inference (RNAi) to increase protein production in the baculovirus expression vector system (BEVS). Spodoptera frugiperda (fall armyworm) (Sf)-caspase...

    Authors: Yiu-Kay Lai, John T-A Hsu, Chih-Chieh Chu, Teng-Yuan Chang, Kao-Lu Pan and Chih-Chien Lin

    Citation: BMC Biotechnology 2012 12:83

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  38. Content type: Research article

    Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction met...

    Authors: Isabelle Houbracken, Luc Baeyens, Philippe Ravassard, Harry Heimberg and Luc Bouwens

    Citation: BMC Biotechnology 2012 12:74

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  39. Content type: Methodology article

    Gene targeting is a powerful method that can be used for examining the functions of genes. Traditionally, the construction of knockout (KO) vectors requires an amplification step to obtain two homologous, larg...

    Authors: Yi Liu, Shangze Li, Huihui Zhang, Zurong Wan, Xiaodong Zhang and Runlei Du

    Citation: BMC Biotechnology 2012 12:71

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  40. Content type: Methodology article

    Virus rescue from transfected cells is an extremely useful technique that allows defined viral clones to be engineered for the purpose of rational vaccine design or fundamental reverse genetics studies. Howeve...

    Authors: Vincent Bourret, Jon Lyall, Mariette F Ducatez, Jean-Luc Guérin and Laurence Tiley

    Citation: BMC Biotechnology 2012 12:69

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  41. Content type: Methodology article

    Cloning vectors capable of retroviral transduction have enabled stable gene overexpression in numerous mitotic cell lines. However, the relatively small number of feasible restriction enzyme sequences in their...

    Authors: Asmita Patel, Anisleidys Muñoz, Katherine Halvorsen and Priyamvada Rai

    Citation: BMC Biotechnology 2012 12:3

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  42. Content type: Methodology article

    Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silence...

    Authors: Eriko Kage-Nakadai, Hiroyuki Kobuna, Osamu Funatsu, Muneyoshi Otori, Keiko Gengyo-Ando, Sawako Yoshina, Sayaka Hori and Shohei Mitani

    Citation: BMC Biotechnology 2012 12:1

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  43. Content type: Methodology article

    Physical mapping of transgenic insertions by Fluorescence in situ Hybridization (FISH) is a reliable and cost-effective technique. Chromosomal assignment is commonly achieved either by concurrent G-banding or ...

    Authors: Mohammed Yusuf, David LV Bauer, Daniel M Lipinski, Robert E MacLaren, Richard Wade-Martins, Kalim U Mir and Emanuela V Volpi

    Citation: BMC Biotechnology 2011 11:121

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