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BMC Biotechnology

Genetic engineering in animals and animal cell systems

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  1. Content type: Research article

    Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits a highly restricted tropism for cells of the monocyte-macrophage lineage, utilizing porcine CD163 (pCD163) as an indispensable cellular rece...

    Authors: Liangliang Li, Chunyan Wu, Gaopeng Hou, Biyun Xue, Sha Xie, Qin Zhao, Yuchen Nan, Gaiping Zhang and En-Min Zhou

    Citation: BMC Biotechnology 2017 17:77

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  2. Content type: Research article

    Pardosa pseudoannulata is a prevailing spider species, and has been regarded as an important bio-control agent of insect pests in farmland of China. However, the available genomic and ...

    Authors: Rong Li, Zhenzhen Yan, Juan Wang, Qisheng Song and Zhi Wang

    Citation: BMC Biotechnology 2017 17:73

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  3. Content type: Research article

    As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cel...

    Authors: Fatemeh Nematpour, Fereidoun Mahboudi, Behrouz Vaziri, Vahid Khalaj, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat and Fatemeh Davami

    Citation: BMC Biotechnology 2017 17:18

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  4. Content type: Research article

    Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter d...

    Authors: Steven C. L. Ho, Esther Y. C. Koh, Benjamin P. C. Soo, Mariati, Sheng-Hao Chao and Yuansheng Yang

    Citation: BMC Biotechnology 2016 16:71

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  5. Content type: Research article

    Diabetes and its concurrent complications impact a significant proportion of the population of the US and create a large financial burden on the American health care system. FDA-approved maggot debridement the...

    Authors: Rebecca J. Linger, Esther J. Belikoff, Ying Yan, Fang Li, Holly A. Wantuch, Helen L. Fitzsimons and Maxwell J. Scott

    Citation: BMC Biotechnology 2016 16:30

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  6. Content type: Research

    Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. However, the low natural abundance of IGF-I limits it...

    Authors: Shujie Liu, Yongming Li, Bo Deng and Ziwei Xu

    Citation: BMC Biotechnology 2016 16:25

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  7. Content type: Research article

    The CRISPR/Cas9 genome editing system has greatly facilitated and expanded our capacity to engineer mammalian genomes, including targeted gene knock-outs. However, the phenotyping of the knock-out effect requi...

    Authors: C. Liesche, L. Venkatraman, S. Aschenbrenner, S. Grosse, D. Grimm, R. Eils and J. Beaudouin

    Citation: BMC Biotechnology 2016 16:17

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  8. Content type: Methodology article

    The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the...

    Authors: Van Trung Chu, Timm Weber, Robin Graf, Thomas Sommermann, Kerstin Petsch, Ulrike Sack, Pavel Volchkov, Klaus Rajewsky and Ralf Kühn

    Citation: BMC Biotechnology 2016 16:4

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  9. Content type: Research article

    Rational design of AAV capsids is a simple method for enhancing AAV transduction efficiency. AAV-DJ is a highly recombinogenic hybrid vector created from DNA shuffling of eight AAV serotypes, which mediates ef...

    Authors: Yingying Mao, Xuejun Wang, Renhe Yan, Wei Hu, Andrew Li, Shengqi Wang and Hongwei Li

    Citation: BMC Biotechnology 2016 16:1

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  10. Content type: Methodology article

    The demethylation potential of environmental pollutants is possibly an innate part of their comprehensive health risk. This paper develops a novel method called TDQ to quantify the demethylation epigenetic tox...

    Authors: Yan Qian, Xiaoli Wang, Zhanlu Lv, Chen Guo, Mei Han, Jiabing Wu, Yongjian Yang, Yishu Yang, Yan Jiang, Yongjie Wei, Jing Nie, Bao Liang, Jinliang Zhang and Xianliang Wang

    Citation: BMC Biotechnology 2015 15:107

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  11. Content type: Methodology article

    The cosmopolitan microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have its complete genome sequenced, an unpre...

    Authors: Chizue Hiruta, Yukiko Ogino, Tetsushi Sakuma, Kenji Toyota, Shinichi Miyagawa, Takashi Yamamoto and Taisen Iguchi

    Citation: BMC Biotechnology 2014 14:95

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  12. Content type: Research article

    Follistatin (FST) has been shown to bind to some TGF-β family members and can function as a potent myostatin (MSTN) antagonist. Recent studies have revealed that over-expression of FST by adeno-associated viru...

    Authors: Mahmood Nazari, Fatemeh Salabi, Li Zhang, Fuping Zhao, Caihong Wei and Lixin Du

    Citation: BMC Biotechnology 2014 14:87

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  13. Content type: Methodology article

    Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, ...

    Authors: Rajiv Kumar Yadav, Florian Barbi, Antoine Ziller, Patricia Luis, Roland Marmeisse, M Sudhakara Reddy and Laurence Fraissinet-Tachet

    Citation: BMC Biotechnology 2014 14:80

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  14. Content type: Research article

    During development of recombinant monoclonal antibodies in Chinese hamster ovary (CHO) cells, C-terminal amidated species are observed. C-terminal amidation is catalysed by peptidylglycine α-amidating monooxyg...

    Authors: Mihaela Škulj, Dejan Pezdirec, Dominik Gaser, Marko Kreft and Robert Zorec

    Citation: BMC Biotechnology 2014 14:76

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  15. Content type: Methodology article

    Microinjection of clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-related RNA and DNA into fertilized eggs is a novel approach for creating gene-modified m...

    Authors: Takayuki Sakurai, Satoshi Watanabe, Akiko Kamiyoshi, Masahiro Sato and Takayuki Shindo

    Citation: BMC Biotechnology 2014 14:69

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  16. Content type: Methodology article

    Establishing highly productive clonal cell lines with constant productivity over 2–3 months of continuous culture remains a tedious task requiring the screening of tens of thousands of clonal colonies. In addi...

    Authors: Nadezhda A Orlova, Sergey V Kovnir, Julia A Hodak, Ivan I Vorobiev, Alexandre G Gabibov and Konstantin G Skryabin

    Citation: BMC Biotechnology 2014 14:56

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  17. Content type: Research article

    High recombinant protein productivity in mammalian cell lines is often associated with phenotypic changes in protein content, energy metabolism, and cell growth, but the key determinants that regulate producti...

    Authors: Raihana Edros, Susan McDonnell and Mohamed Al-Rubeai

    Citation: BMC Biotechnology 2014 14:15

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