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Cellular and tissue engineering

Section edited by Joaquim Cabral

This section will consider articles that relate to stem cell research and regenerative medicine as well as cell culture methods and bioproduction in animal cells.


  1. Content type: Research article

    MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derive...

    Authors: Song Chen, Zhenyu Xu, Jiahua Shao, Peiliang Fu and Haishan Wu

    Citation: BMC Biotechnology 2019 19:6

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  2. Content type: Methodology article

    Tissue-engineered cardiac constructs have potential in the functional recovery of heart failure; however, the preservation of these constructs is crucial for the development and widespread application of this ...

    Authors: Hirotatsu Ohkawara, Shigeru Miyagawa, Satsuki Fukushima, Shin Yajima, Atsuhiro Saito, Hiroshi Nagashima and Yoshiki Sawa

    Citation: BMC Biotechnology 2018 18:56

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  3. Content type: Research article

    Dedifferentiation of chondrocytes during cell expansion is one of the barriers in tissue construction for cartilage repair. To understand chondrocyte behavior and improve cell expansion in monolayer culture, t...

    Authors: Sopita Wongin, Saranatra Waikakul, Pojchong Chotiyarnwong, Wanwipa Siriwatwechakul, Masahiro Kino-oka, Mee-Hae Kim and Kwanchanok Viravaidya-Pasuwat

    Citation: BMC Biotechnology 2018 18:14

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  4. Content type: Research article

    The concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce ...

    Authors: Agnieszka J. Turlo, Yalda Ashraf Kharaz, Peter D. Clegg, James Anderson and Mandy J. Peffers

    Citation: BMC Biotechnology 2018 18:2

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  5. Content type: Methodology article

    Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning meth...

    Authors: Jacob J. Messner, Honor L. Glenn and Deirdre R. Meldrum

    Citation: BMC Biotechnology 2017 17:89

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  6. Content type: Research article

    Umbilical cord blood provides a source of hematopoietic stem cells for transplantation with immunological and availability advantages over conventional bone marrow sources. Limited cell numbers and slower engr...

    Authors: Rebecca L. L. Moore, Matthew J. Worrallo, Peter D. Mitchell, Jon Harriman, Katie E. Glen and Robert J. Thomas

    Citation: BMC Biotechnology 2017 17:65

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  7. Content type: Methodology article

    Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated...

    Authors: Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura and Yoshihiro Nakajima

    Citation: BMC Biotechnology 2017 17:54

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  9. Content type: Research article

    Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastas...

    Authors: Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn and Thomas Böldicke

    Citation: BMC Biotechnology 2017 17:42

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  10. Content type: Research article

    Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which t...

    Authors: Nadezhda A. Orlova, Sergey V. Kovnir, Alexandre G. Gabibov and Ivan I. Vorobiev

    Citation: BMC Biotechnology 2017 17:33

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  11. Content type: Research article

    Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Authors: Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    Citation: BMC Biotechnology 2017 17:17

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  12. Content type: Research article

    Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Authors: Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    Citation: BMC Biotechnology 2017 17:14

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  13. Content type: Methodology article

    Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Authors: Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    Citation: BMC Biotechnology 2017 17:13

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  14. Content type: Research article

    Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Authors: Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    Citation: BMC Biotechnology 2017 17:11

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  15. Content type: Research article

    The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Authors: Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    Citation: BMC Biotechnology 2016 16:89

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  17. Content type: Research article

    The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA)...

    Authors: Yanxia Zhu, Xiaomin Wu, Yuhong Liang, Hongsheng Gu, Kedong Song, Xuenong Zou and Guangqian Zhou

    Citation: BMC Biotechnology 2016 16:78

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  18. Content type: Research article

    Tissue engineering and bone substitutes are subjects of intensive ongoing research. If the healing of bone fractures is delayed, osteoinductive materials that induce mesenchymal stem cells (MSCs) to form bone ...

    Authors: Steffen Kissling, Michael Seidenstuecker, Ingo H. Pilz, Norbert P. Suedkamp, Hermann O. Mayr and Anke Bernstein

    Citation: BMC Biotechnology 2016 16:44

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  19. Content type: Research article

    Fetal bovine serum (FBS) contains a wide range of growth factors, hormones, vitamins, amino acids, fatty acids and trace elements required for cell growth. It was shown that animal sera contain also extracellu...

    Authors: Hala Aswad, Audrey Jalabert and Sophie Rome

    Citation: BMC Biotechnology 2016 16:32

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  20. Content type: Research article

    von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Intere...

    Authors: Hee Young Yoo, Young Hoon Song, Mathias Foo, Eunseok Seo, Dong Soo Hwang and Jeong Hyun Seo

    Citation: BMC Biotechnology 2016 16:16

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  21. Content type: Research article

    Mesenchymal stem cells (MSCs) are viewed as a having significant potential for tissue engineering and regenerative medicine therapies. Clinical implementation of MSCs, however, demands that their preparation b...

    Authors: Stephen M. Goldman and Gilda A. Barabino

    Citation: BMC Biotechnology 2016 16:10

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  22. Content type: Research article

    Recombinant cell lines developed for therapeutic antibody production often suffer instability or lose recombinant protein expression during long-term culture. Heterogeneous gene expression among cell line subc...

    Authors: Nathalie Veith, Holger Ziehr, Roderick A. F. MacLeod and Stella Marie Reamon-Buettner

    Citation: BMC Biotechnology 2016 16:6

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  23. Content type: Research article

    We aimed to demonstrate that DF stem cells from impacted molars and canines can be used to improve bone regeneration on titanium implants surfaces. This study highlights the presence of stem cells in DF, their...

    Authors: Ondine Lucaciu, Olga Soriţău, Dan Gheban, Dan Rus Ciuca, Oana Virtic, Adriana Vulpoi, Noemi Dirzu, Radu Câmpian, Grigore Băciuţ, Catalin Popa, Simion Simon, Petru Berce, Mihaela Băciuţ and Bogdan Crisan

    Citation: BMC Biotechnology 2015 15:114

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  24. Content type: Research article

    Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the tra...

    Authors: Asha Shekaran, Eileen Sim, Kah Yong Tan, Jerry Kok Yen Chan, Mahesh Choolani, Shaul Reuveny and Steve Oh

    Citation: BMC Biotechnology 2015 15:102

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  25. Content type: Research article

    Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. Th...

    Authors: Uros Jamnikar, Petra Nikolic, Ales Belic, Marjanca Blas, Dominik Gaser, Andrej Francky, Holger Laux, Andrej Blejec, Spela Baebler and Kristina Gruden

    Citation: BMC Biotechnology 2015 15:98

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  27. Content type: Research article

    The Arterovenous Loop (AV Loop) model is a vascularization model in tissue engineering research, which is capable of generating a three dimensional in vivo unit with cells as well as the supporting vessels within...

    Authors: Quan Yuan, Oliver Bleiziffer, Anja M Boos, Jiaming Sun, Andreas Brandl, Justus P Beier, Andreas Arkudas, Marweh Schmitz, Ulrich Kneser and Raymund E Horch

    Citation: BMC Biotechnology 2014 14:112

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  28. Content type: Research article

    Cartilage tissue engineering offers new strategies in repairing damaged cartilage. Scaffolds have been used for the in vitro and in vivo procedures for this application, which demonstrates the compatible biologic...

    Authors: Jeerawan Klangjorhor, Thanyaluck Phitak, Dumnoensun Pruksakorn, Peraphan Pothacharoen and Prachya Kongtawelert

    Citation: BMC Biotechnology 2014 14:108

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  29. Content type: Research article

    Mesenchymal stem cells (MSCs) are a favored cell source for regenerative medicine because of their multilinage potential. However, the conventional monolayer technique used to culture MSCs, inadequately overco...

    Authors: Yuichiro Yamaguchi, Jun Ohno, Ayako Sato, Hirofumi Kido and Tadao Fukushima

    Citation: BMC Biotechnology 2014 14:105

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  31. Content type: Research article

    Myocardial infarction remains the leading cause of mortality in developed countries despite recent advances in its prevention and treatment. Regenerative therapies based on resident cardiac progenitor cells (C...

    Authors: Hao Wang, Hao Chen, Bei Feng, Xiang Wang, Xiaomin He, Renjie Hu, Meng Yin, Wei Wang, Wei Fu and Zhiwei Xu

    Citation: BMC Biotechnology 2014 14:75

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  32. Content type: Research article

    In human vaccine manufacturing some pathogens such as Modified Vaccinia Virus Ankara, measles, mumps virus as well as influenza viruses are still produced on primary material derived from embryonated chicken e...

    Authors: Verena Lohr, Oliver Hädicke, Yvonne Genzel, Ingo Jordan, Heino Büntemeyer, Steffen Klamt and Udo Reichl

    Citation: BMC Biotechnology 2014 14:72

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  33. Content type: Research article

    Japanese encephalitis virus (JEV) is the most important cause of epidemic encephalitis in most Asian regions. There is no specific treatment available for Japanese encephalitis, and vaccination is the only eff...

    Authors: Rong-Hong Hua, Ye-Nan Li, Zhen-Shi Chen, Li-Ke Liu, Hong Huo, Xiao-Lei Wang, Li-Ping Guo, Nan Shen, Jing-Fei Wang and Zhi-Gao Bu

    Citation: BMC Biotechnology 2014 14:62

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  34. Content type: Methodology article

    In this study, we investigated the effect of the extracellular matrix on endothelial dysfunction by careful observation of human umbilical vein endothelial cells (HUVECs) cultured on denatured collagen film.

    Authors: Eunseok Seo, Kyung Won Seo, Jung-Eun Gil, Young-Ran Ha, Eunseop Yeom, Seungchul Lee and Sang Joon Lee

    Citation: BMC Biotechnology 2014 14:61

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  35. Content type: Research article

    Green fluorescent protein (GFP) is a useful biomarker, widely used in biomedical research to track stem cells after transplantation and/or to assess therapeutic transgene expression. However, both GFP and ther...

    Authors: Zhong Yang, Yaming Wang, Yanan Li, Qiang Liu, Qing Zeng and Xiaoyin Xu

    Citation: BMC Biotechnology 2014 14:55

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  36. Content type: Research article

    Intramuscular fat deposition in the meat animal is relatively new strategy for developing the meat quality. Fat deposition is largely depending on the adipocyte proliferation and differentiation. Therefore, we...

    Authors: Soundarrajan Ilavenil, Mariadhas Valan Arasu, Jeong-Chae Lee, Da Hye Kim, Mayakrishnan Vijayakumar, Kyung Dong Lee and Ki Choon Choi

    Citation: BMC Biotechnology 2014 14:54

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  37. Content type: Research article

    Cell-based regeneration therapies have great potential for application in new areas in clinical medicine, although some obstacles still remain to be overcome for a wide range of clinical applications. One majo...

    Authors: Daisuke Kami, Keizo Watakabe, Mayu Yamazaki-Inoue, Kahori Minami, Tomoya Kitani, Yoko Itakura, Masashi Toyoda, Takashi Sakurai, Akihiro Umezawa and Satoshi Gojo

    Citation: BMC Biotechnology 2013 13:102

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  38. Content type: Research article

    Normal development and the response to injury both require cell growth, migration and morphological remodeling, guided by a complex local landscape of permissive and inhibitory cues. A standard approach for st...

    Authors: Wiam Belkaid, Peter Thostrup, Patricia T Yam, Camille A Juzwik, Edward S Ruthazer, Ajit S Dhaunchak and David R Colman

    Citation: BMC Biotechnology 2013 13:86

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  40. Content type: Research article

    Common cell based strategies for the treatment of osseous defects require the isolation and expansion of autologous cells. Since this makes such approaches time-consuming and expensive, we developed a novel ex...

    Authors: Oliver B Betz, Volker M Betz, Christian Schröder, Rainer Penzkofer, Michael Göttlinger, Susanne Mayer-Wagner, Peter Augat, Volkmar Jansson and Peter E Müller

    Citation: BMC Biotechnology 2013 13:65

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  41. Content type: Methodology article

    There is considerable interest in using cell sheets for the treatment of various lesions as part of regenerative medicine therapy. Cell sheets can be prepared in temperature-responsive culture dishes and appli...

    Authors: Miki Maehara, Masato Sato, Masahito Watanabe, Hitomi Matsunari, Mami Kokubo, Takahiro Kanai, Michio Sato, Kazuaki Matsumura, Suong-Hyu Hyon, Munetaka Yokoyama, Joji Mochida and Hiroshi Nagashima

    Citation: BMC Biotechnology 2013 13:58

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  42. Content type: Research article

    A key issue for safe and reproducible gene therapy approaches is the autologous and tissue-specific expression of transgenes. Tissue-specific expression in vivo is either achieved by transfer vectors that deli...

    Authors: Rudolf Haase, Terese Magnusson, Baowei Su, Florian Kopp, Ernst Wagner, Hans Lipps, Armin Baiker and Manfred Ogris

    Citation: BMC Biotechnology 2013 13:49

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  43. Content type: Research article

    There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-...

    Authors: Qi Wei, Daniel Reidler, Min Ye Shen and Hayden Huang

    Citation: BMC Biotechnology 2013 13:17

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  44. Content type: Research article

    Guanylate binding protein-1 (GBP-1) is a large GTPase which is actively secreted by endothelial cells. It is a marker and intracellular inhibitor of endothelial cell proliferation, migration, and invasion. We ...

    Authors: Oliver Bleiziffer, Matthias Hammon, Andreas Arkudas, Christian D Taeger, Justus P Beier, Kerstin Amann, Elisabeth Naschberger, Michael Stürzl, Raymund E Horch and Ulrich Kneser

    Citation: BMC Biotechnology 2012 12:94

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  45. Content type: Research article

    Despite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive.

    Authors: Bert R Everaert, Irene Bergwerf, Nathalie De Vocht, Peter Ponsaerts, Annemie Van Der Linden, Jean-Pierre Timmermans and Christiaan J Vrints

    Citation: BMC Biotechnology 2012 12:93

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  46. Content type: Methodology article

    Induced pluripotent stem (iPS) cells can differentiate into any cell type, which makes them an attractive resource in fields such as regenerative medicine, drug screening, or in vitro toxicology. The most importa...

    Authors: Hiroyuki Koike, Koji Kubota, Keisuke Sekine, Takanori Takebe, Rie Ouchi, Yun-Wen Zheng, Yasuharu Ueno, Naoki Tanigawa and Hideki Taniguchi

    Citation: BMC Biotechnology 2012 12:81

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  47. Content type: Research article

    In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions fo...

    Authors: Gesine Pless-Petig, Martin Metzenmacher, Tobias R Türk and Ursula Rauen

    Citation: BMC Biotechnology 2012 12:73

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  48. Content type: Research article

    Integrin-mediated interaction of neuronal cells with extracellular matrix (ECM) is important for the control of cell adhesion, morphology, motility, and differentiation in both in vitro and in vivo systems. Arg-G...

    Authors: Won Bae Jeon, Bo Hyung Park, Seong Kyoon Choi, Kyeong-Min Lee and Jin-Kyu Park

    Citation: BMC Biotechnology 2012 12:61

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  49. Content type: Methodology article

    Achieving efficient introduction of plasmid DNA into primary cultures of mammalian cells is a common problem in biomedical research. Human primary cranial suture cells are derived from the connective mesenchym...

    Authors: Prem P Dwivedi, Peter J Anderson and Barry C Powell

    Citation: BMC Biotechnology 2012 12:45

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  50. Content type: Research article

    Diabetes mellitus is a complicated disease with a pathophysiology that includes hyperinsulinemia, hyperglycemia and other metabolic impairments leading to many clinical complications. It is necessary to develo...

    Authors: Mina Rasouli, Zalinah Ahmad, Abdul Rahman Omar and Zeenathul N Allaudin

    Citation: BMC Biotechnology 2011 11:99

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