Cellular and tissue engineering

Section edited by Joaquim Cabral

This section will consider articles that relate to stem cell research and regenerative medicine as well as cell culture methods and bioproduction in animal cells.

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  1. Research article

    Immobilisation of Delta-like 1 ligand for the scalable and controlled manufacture of hematopoietic progenitor cells in a stirred bioreactor

    Umbilical cord blood provides a source of hematopoietic stem cells for transplantation with immunological and availability advantages over conventional bone marrow sources. Limited cell numbers and slower engr...

    Rebecca L. L. Moore, Matthew J. Worrallo, Peter D. Mitchell, Jon Harriman, Katie E. Glen and Robert J. Thomas

    BMC Biotechnology 2017 17:65

    Published on: 4 August 2017

  2. Methodology article

    Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

    Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated...

    Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura and Yoshihiro Nakajima

    BMC Biotechnology 2017 17:54

    Published on: 20 June 2017

  3. Research article

    Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells

    Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastas...

    Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn and Thomas Böldicke

    BMC Biotechnology 2017 17:42

    Published on: 12 May 2017

  4. Research article

    Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

    Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which t...

    Nadezhda A. Orlova, Sergey V. Kovnir, Alexandre G. Gabibov and Ivan I. Vorobiev

    BMC Biotechnology 2017 17:33

    Published on: 24 March 2017

  5. Research article

    Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system

    Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    BMC Biotechnology 2017 17:17

    Published on: 20 February 2017

  6. Research article

    Mild electrical stimulation with heat shock guides differentiation of embryonic stem cells into Pdx1-expressing cells within the definitive endoderm

    Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    BMC Biotechnology 2017 17:14

    Published on: 15 February 2017

  7. Methodology article

    Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study

    Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    BMC Biotechnology 2017 17:13

    Published on: 14 February 2017

  8. Research article

    Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters

    Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    BMC Biotechnology 2017 17:11

    Published on: 13 February 2017

  9. Research article

    Means of enhancing bone fracture healing: optimal cell source, isolation methods and acoustic stimulation

    The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    BMC Biotechnology 2016 16:89

    Published on: 12 December 2016

  10. Research article

    Repair of cartilage defects in osteoarthritis rats with induced pluripotent stem cell derived chondrocytes

    The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA)...

    Yanxia Zhu, Xiaomin Wu, Yuhong Liang, Hongsheng Gu, Kedong Song, Xuenong Zou and Guangqian Zhou

    BMC Biotechnology 2016 16:78

    Published on: 9 November 2016

  11. Research article

    Sustained release of rhBMP-2 from microporous tricalciumphosphate using hydrogels as a carrier

    Tissue engineering and bone substitutes are subjects of intensive ongoing research. If the healing of bone fractures is delayed, osteoinductive materials that induce mesenchymal stem cells (MSCs) to form bone ...

    Steffen Kissling, Michael Seidenstuecker, Ingo H. Pilz, Norbert P. Suedkamp, Hermann O. Mayr and Anke Bernstein

    BMC Biotechnology 2016 16:44

    Published on: 20 May 2016

  12. Research article

    Hydrodynamic loading in concomitance with exogenous cytokine stimulation modulates differentiation of bovine mesenchymal stem cells towards osteochondral lineages

    Mesenchymal stem cells (MSCs) are viewed as a having significant potential for tissue engineering and regenerative medicine therapies. Clinical implementation of MSCs, however, demands that their preparation b...

    Stephen M. Goldman and Gilda A. Barabino

    BMC Biotechnology 2016 16:10

    Published on: 1 February 2016

  13. Research article

    Dental follicle stem cells in bone regeneration on titanium implants

    We aimed to demonstrate that DF stem cells from impacted molars and canines can be used to improve bone regeneration on titanium implants surfaces. This study highlights the presence of stem cells in DF, their...

    Ondine Lucaciu, Olga Soriţău, Dan Gheban, Dan Rus Ciuca, Oana Virtic, Adriana Vulpoi, Noemi Dirzu, Radu Câmpian, Grigore Băciuţ, Catalin Popa, Simion Simon, Petru Berce, Mihaela Băciuţ and Bogdan Crisan

    BMC Biotechnology 2015 15:114

    Published on: 30 December 2015

  14. Research article

    Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers

    Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the tra...

    Asha Shekaran, Eileen Sim, Kah Yong Tan, Jerry Kok Yen Chan, Mahesh Choolani, Shaul Reuveny and Steve Oh

    BMC Biotechnology 2015 15:102

    Published on: 31 October 2015

  15. Research article

    Transcriptome study and identification of potential marker genes related to the stable expression of recombinant proteins in CHO clones

    Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. Th...

    Uros Jamnikar, Petra Nikolic, Ales Belic, Marjanca Blas, Dominik Gaser, Andrej Francky, Holger Laux, Andrej Blejec, Spela Baebler and Kristina Gruden

    BMC Biotechnology 2015 15:98

    Published on: 23 October 2015

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