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Cellular and tissue engineering

This section will consider articles that relate to stem cell research and regenerative medicine as well as cell culture methods and bioproduction in animal cells.


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  1. Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media ...

    Authors: Yijiang Huang, Daniel Seitz, Yan Chevalier, Peter E. Müller, Volkmar Jansson and Roland M. Klar

    Citation: BMC Biotechnology 2020 20:48

    Content type: Research article

    Published on:

  2. Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) us...

    Authors: Young-Hoon Jeong, Ukjin Kim, Seul-Gi Lee, Bokyeong Ryu, Jin Kim, Artyuhov Igor, Jong Soo Kim, Cho-Rok Jung, Jae-Hak Park and C-Yoon Kim

    Citation: BMC Biotechnology 2020 20:45

    Content type: Research article

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  3. Oral administration is the most common way to deliver drugs to the systemic circulation or target organs. Orally administered drugs are absorbed in the intestine and metabolized in the intestine and liver. In ...

    Authors: Yumi Ohta, Kanako Kazuki, Satoshi Abe, Mitsuo Oshimura, Kaoru Kobayashi and Yasuhiro Kazuki

    Citation: BMC Biotechnology 2020 20:44

    Content type: Research article

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  4. The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternati...

    Authors: Tao He, Jörg Hausdorf, Yan Chevalier and Roland M. Klar

    Citation: BMC Biotechnology 2020 20:8

    Content type: Research article

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  5. Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the ze...

    Authors: David Simon Kleinhans and Virginie Lecaudey

    Citation: BMC Biotechnology 2019 19:68

    Content type: Methodology article

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  6. Engineered therapeutic cells have attracted a great deal of interest due to their potential applications in treating a wide range of diseases, including cancer and autoimmunity. Chimeric antigen receptor (CAR)...

    Authors: Alexandre Juillerat, Diane Tkach, Brian W. Busser, Sonal Temburni, Julien Valton, Aymeric Duclert, Laurent Poirot, Stéphane Depil and Philippe Duchateau

    Citation: BMC Biotechnology 2019 19:44

    Content type: Research article

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  7. Artificial Mitochondrial Transfer or Transplant (AMT/T) can be used to reduce the stress and loss of viability of damaged cells. In MitoCeption, a type of AMT/T, the isolated mitochondria and recipient cells a...

    Authors: Francisco Cabrera, Mayra Ortega, Francesca Velarde, Eliseo Parra, Stephany Gallardo, Diego Barba, Lina Soto, Gabriela Peña, Luis Alberto Pedroza, Christian Jorgensen, Maroun Khoury and Andrés Caicedo

    Citation: BMC Biotechnology 2019 19:42

    Content type: Research article

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  9. The gene transduction efficiency of adenovirus to hematopoietic cells, especially T lymphocytes, is needed to be improved. The purpose of this study is to improve the transduction efficiency of T lymphocytes b...

    Authors: Yun Lv, Feng-Jun Xiao, Yi Wang, Xiao-Hui Zou, Hua Wang, Hai-Yan Wang, Li-Sheng Wang and Zhuo-Zhuang Lu

    Citation: BMC Biotechnology 2019 19:23

    Content type: Research article

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  10. A robust scalable method for producing enucleated red blood cells (RBCs) is not only a process to produce packed RBC units for transfusion but a potential platform to produce modified RBCs with applications in...

    Authors: Steven Mayers, Pablo Diego Moço, Talha Maqbool, Pamuditha N. Silva, Dawn M. Kilkenny and Julie Audet

    Citation: BMC Biotechnology 2019 19:21

    Content type: Research article

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  11. Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC...

    Authors: Guiqi Zhang, Yi Liu, Lichen Xu, Chunhe Sha, Haibin Zhang and Weibing Xu

    Citation: BMC Biotechnology 2019 19:10

    Content type: Research article

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  12. MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derive...

    Authors: Song Chen, Zhenyu Xu, Jiahua Shao, Peiliang Fu and Haishan Wu

    Citation: BMC Biotechnology 2019 19:6

    Content type: Research article

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  13. Tissue-engineered cardiac constructs have potential in the functional recovery of heart failure; however, the preservation of these constructs is crucial for the development and widespread application of this ...

    Authors: Hirotatsu Ohkawara, Shigeru Miyagawa, Satsuki Fukushima, Shin Yajima, Atsuhiro Saito, Hiroshi Nagashima and Yoshiki Sawa

    Citation: BMC Biotechnology 2018 18:56

    Content type: Methodology article

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  14. Dedifferentiation of chondrocytes during cell expansion is one of the barriers in tissue construction for cartilage repair. To understand chondrocyte behavior and improve cell expansion in monolayer culture, t...

    Authors: Sopita Wongin, Saranatra Waikakul, Pojchong Chotiyarnwong, Wanwipa Siriwatwechakul, Masahiro Kino-oka, Mee-Hae Kim and Kwanchanok Viravaidya-Pasuwat

    Citation: BMC Biotechnology 2018 18:14

    Content type: Research article

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  15. The concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce ...

    Authors: Agnieszka J. Turlo, Yalda Ashraf Kharaz, Peter D. Clegg, James Anderson and Mandy J. Peffers

    Citation: BMC Biotechnology 2018 18:2

    Content type: Research article

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  16. Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning meth...

    Authors: Jacob J. Messner, Honor L. Glenn and Deirdre R. Meldrum

    Citation: BMC Biotechnology 2017 17:89

    Content type: Methodology article

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  17. Umbilical cord blood provides a source of hematopoietic stem cells for transplantation with immunological and availability advantages over conventional bone marrow sources. Limited cell numbers and slower engr...

    Authors: Rebecca L. L. Moore, Matthew J. Worrallo, Peter D. Mitchell, Jon Harriman, Katie E. Glen and Robert J. Thomas

    Citation: BMC Biotechnology 2017 17:65

    Content type: Research article

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  18. Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated...

    Authors: Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura and Yoshihiro Nakajima

    Citation: BMC Biotechnology 2017 17:54

    Content type: Methodology article

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  20. Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastas...

    Authors: Stefan Somplatzki, Martina Mühlenhoff, Andrea Kröger, Rita Gerardy-Schahn and Thomas Böldicke

    Citation: BMC Biotechnology 2017 17:42

    Content type: Research article

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  21. Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which t...

    Authors: Nadezhda A. Orlova, Sergey V. Kovnir, Alexandre G. Gabibov and Ivan I. Vorobiev

    Citation: BMC Biotechnology 2017 17:33

    Content type: Research article

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  22. Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Authors: Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    Citation: BMC Biotechnology 2017 17:17

    Content type: Research article

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  23. Because of the increasing number of diabetic patients, it is important to generate pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing cells, which are capable of differentiating into pancreatic endocrin...

    Authors: Tomoaki Koga, Nobuaki Shiraki, Shuichiro Yano, Mary Ann Suico, Saori Morino-Koga, Takashi Sato, Tsuyoshi Shuto, Shoen Kume and Hirofumi Kai

    Citation: BMC Biotechnology 2017 17:14

    Content type: Research article

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  24. Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of e...

    Authors: Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller and Janina Burk

    Citation: BMC Biotechnology 2017 17:13

    Content type: Methodology article

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  25. Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exoge...

    Authors: Hao Chen, Qisheng Zuo, Yingjie Wang, Jiuzhou Song, Huilin Yang, Yani Zhang and Bichun Li

    Citation: BMC Biotechnology 2017 17:11

    Content type: Research article

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  26. The human body has an extensive capacity to regenerate bone tissue after trauma. However large defects such as long bone fractures of the lower limbs cannot be restored without intervention and often lead to n...

    Authors: Corina Adriana Ghebes, Maaike Vera Jasmijn Braham, Adelgunde Veronica Clemens Maria Zeegers, Auke Jan Sijbe Renard, Hugo Fernandes and Daniel B F Saris

    Citation: BMC Biotechnology 2016 16:89

    Content type: Research article

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  28. The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA)...

    Authors: Yanxia Zhu, Xiaomin Wu, Yuhong Liang, Hongsheng Gu, Kedong Song, Xuenong Zou and Guangqian Zhou

    Citation: BMC Biotechnology 2016 16:78

    Content type: Research article

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  29. Tissue engineering and bone substitutes are subjects of intensive ongoing research. If the healing of bone fractures is delayed, osteoinductive materials that induce mesenchymal stem cells (MSCs) to form bone ...

    Authors: Steffen Kissling, Michael Seidenstuecker, Ingo H. Pilz, Norbert P. Suedkamp, Hermann O. Mayr and Anke Bernstein

    Citation: BMC Biotechnology 2016 16:44

    Content type: Research article

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  30. Fetal bovine serum (FBS) contains a wide range of growth factors, hormones, vitamins, amino acids, fatty acids and trace elements required for cell growth. It was shown that animal sera contain also extracellu...

    Authors: Hala Aswad, Audrey Jalabert and Sophie Rome

    Citation: BMC Biotechnology 2016 16:32

    Content type: Research article

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  31. von Willebrand factor (VWF) is a key load bearing domain for mamalian cell adhesion by binding various macromolecular ligands in extracellular matrix such as, collagens, elastin, and glycosaminoglycans. Intere...

    Authors: Hee Young Yoo, Young Hoon Song, Mathias Foo, Eunseok Seo, Dong Soo Hwang and Jeong Hyun Seo

    Citation: BMC Biotechnology 2016 16:16

    Content type: Research article

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  32. Mesenchymal stem cells (MSCs) are viewed as a having significant potential for tissue engineering and regenerative medicine therapies. Clinical implementation of MSCs, however, demands that their preparation b...

    Authors: Stephen M. Goldman and Gilda A. Barabino

    Citation: BMC Biotechnology 2016 16:10

    Content type: Research article

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  33. Recombinant cell lines developed for therapeutic antibody production often suffer instability or lose recombinant protein expression during long-term culture. Heterogeneous gene expression among cell line subc...

    Authors: Nathalie Veith, Holger Ziehr, Roderick A. F. MacLeod and Stella Marie Reamon-Buettner

    Citation: BMC Biotechnology 2016 16:6

    Content type: Research article

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  34. We aimed to demonstrate that DF stem cells from impacted molars and canines can be used to improve bone regeneration on titanium implants surfaces. This study highlights the presence of stem cells in DF, their...

    Authors: Ondine Lucaciu, Olga Soriţău, Dan Gheban, Dan Rus Ciuca, Oana Virtic, Adriana Vulpoi, Noemi Dirzu, Radu Câmpian, Grigore Băciuţ, Catalin Popa, Simion Simon, Petru Berce, Mihaela Băciuţ and Bogdan Crisan

    Citation: BMC Biotechnology 2015 15:114

    Content type: Research article

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  35. Mesenchymal stem cells (MSCs) are of great interest in bone regenerative medicine due to their osteogenic potential and trophic effects. However, challenges to large-scale production of MSCs can hinder the tra...

    Authors: Asha Shekaran, Eileen Sim, Kah Yong Tan, Jerry Kok Yen Chan, Mahesh Choolani, Shaul Reuveny and Steve Oh

    Citation: BMC Biotechnology 2015 15:102

    Content type: Research article

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  36. Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. Th...

    Authors: Uros Jamnikar, Petra Nikolic, Ales Belic, Marjanca Blas, Dominik Gaser, Andrej Francky, Holger Laux, Andrej Blejec, Spela Baebler and Kristina Gruden

    Citation: BMC Biotechnology 2015 15:98

    Content type: Research article

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  38. The Arterovenous Loop (AV Loop) model is a vascularization model in tissue engineering research, which is capable of generating a three dimensional in vivo unit with cells as well as the supporting vessels within...

    Authors: Quan Yuan, Oliver Bleiziffer, Anja M Boos, Jiaming Sun, Andreas Brandl, Justus P Beier, Andreas Arkudas, Marweh Schmitz, Ulrich Kneser and Raymund E Horch

    Citation: BMC Biotechnology 2014 14:112

    Content type: Research article

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  39. Cartilage tissue engineering offers new strategies in repairing damaged cartilage. Scaffolds have been used for the in vitro and in vivo procedures for this application, which demonstrates the compatible biologic...

    Authors: Jeerawan Klangjorhor, Thanyaluck Phitak, Dumnoensun Pruksakorn, Peraphan Pothacharoen and Prachya Kongtawelert

    Citation: BMC Biotechnology 2014 14:108

    Content type: Research article

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  40. Mesenchymal stem cells (MSCs) are a favored cell source for regenerative medicine because of their multilinage potential. However, the conventional monolayer technique used to culture MSCs, inadequately overco...

    Authors: Yuichiro Yamaguchi, Jun Ohno, Ayako Sato, Hirofumi Kido and Tadao Fukushima

    Citation: BMC Biotechnology 2014 14:105

    Content type: Research article

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  42. Myocardial infarction remains the leading cause of mortality in developed countries despite recent advances in its prevention and treatment. Regenerative therapies based on resident cardiac progenitor cells (C...

    Authors: Hao Wang, Hao Chen, Bei Feng, Xiang Wang, Xiaomin He, Renjie Hu, Meng Yin, Wei Wang, Wei Fu and Zhiwei Xu

    Citation: BMC Biotechnology 2014 14:75

    Content type: Research article

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  43. In human vaccine manufacturing some pathogens such as Modified Vaccinia Virus Ankara, measles, mumps virus as well as influenza viruses are still produced on primary material derived from embryonated chicken e...

    Authors: Verena Lohr, Oliver Hädicke, Yvonne Genzel, Ingo Jordan, Heino Büntemeyer, Steffen Klamt and Udo Reichl

    Citation: BMC Biotechnology 2014 14:72

    Content type: Research article

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  44. Japanese encephalitis virus (JEV) is the most important cause of epidemic encephalitis in most Asian regions. There is no specific treatment available for Japanese encephalitis, and vaccination is the only eff...

    Authors: Rong-Hong Hua, Ye-Nan Li, Zhen-Shi Chen, Li-Ke Liu, Hong Huo, Xiao-Lei Wang, Li-Ping Guo, Nan Shen, Jing-Fei Wang and Zhi-Gao Bu

    Citation: BMC Biotechnology 2014 14:62

    Content type: Research article

    Published on:

  45. In this study, we investigated the effect of the extracellular matrix on endothelial dysfunction by careful observation of human umbilical vein endothelial cells (HUVECs) cultured on denatured collagen film.

    Authors: Eunseok Seo, Kyung Won Seo, Jung-Eun Gil, Young-Ran Ha, Eunseop Yeom, Seungchul Lee and Sang Joon Lee

    Citation: BMC Biotechnology 2014 14:61

    Content type: Methodology article

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  46. Green fluorescent protein (GFP) is a useful biomarker, widely used in biomedical research to track stem cells after transplantation and/or to assess therapeutic transgene expression. However, both GFP and ther...

    Authors: Zhong Yang, Yaming Wang, Yanan Li, Qiang Liu, Qing Zeng and Xiaoyin Xu

    Citation: BMC Biotechnology 2014 14:55

    Content type: Research article

    Published on:

  47. Intramuscular fat deposition in the meat animal is relatively new strategy for developing the meat quality. Fat deposition is largely depending on the adipocyte proliferation and differentiation. Therefore, we...

    Authors: Soundarrajan Ilavenil, Mariadhas Valan Arasu, Jeong-Chae Lee, Da Hye Kim, Mayakrishnan Vijayakumar, Kyung Dong Lee and Ki Choon Choi

    Citation: BMC Biotechnology 2014 14:54

    Content type: Research article

    Published on:

  48. Cell-based regeneration therapies have great potential for application in new areas in clinical medicine, although some obstacles still remain to be overcome for a wide range of clinical applications. One majo...

    Authors: Daisuke Kami, Keizo Watakabe, Mayu Yamazaki-Inoue, Kahori Minami, Tomoya Kitani, Yoko Itakura, Masashi Toyoda, Takashi Sakurai, Akihiro Umezawa and Satoshi Gojo

    Citation: BMC Biotechnology 2013 13:102

    Content type: Research article

    Published on:

  49. Normal development and the response to injury both require cell growth, migration and morphological remodeling, guided by a complex local landscape of permissive and inhibitory cues. A standard approach for st...

    Authors: Wiam Belkaid, Peter Thostrup, Patricia T Yam, Camille A Juzwik, Edward S Ruthazer, Ajit S Dhaunchak and David R Colman

    Citation: BMC Biotechnology 2013 13:86

    Content type: Research article

    Published on:

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