Applied immunology

Section edited by Marko Radic

This section will consider studies on protein or cell engineering as they apply to the functions of the immune system and have applications in diagnostics, therapy and biomedical research.


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  1. Methodology article

    Overview of methodologies for T-cell receptor repertoire analysis

    The T-cell receptor (TCR), located on the surface of T cells, is responsible for the recognition of the antigen-major histocompatibility complex, leading to the initiation of an inflammatory response. Analysin...

    Elisa Rosati, C Marie Dowds, Evaggelia Liaskou, Eva Kristine Klemsdal Henriksen, Tom H Karlsen and Andre Franke

    BMC Biotechnology 2017 17:61

    Published on: 10 July 2017

  2. Research article

    Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression

    The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

    Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

    BMC Biotechnology 2017 17:52

    Published on: 12 June 2017

  3. Research article

    A fast and effective determination of the biodistribution and subcellular localization of fluorescent immunoliposomes in freshly excised animal organs

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    BMC Biotechnology 2017 17:8

    Published on: 18 January 2017

  4. Methodology article

    Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

    There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

    Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

    BMC Biotechnology 2017 17:3

    Published on: 9 January 2017

  5. Research article

    Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

    The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

    Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

    BMC Biotechnology 2016 16:84

    Published on: 24 November 2016

  6. Methodology article

    Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

    Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

    Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

    BMC Biotechnology 2016 16:83

    Published on: 22 November 2016

  7. Research article

    New strategy for testing efficacy of immunotherapeutic compounds for diabetes in vitro

    The valuable role of immunotherapy in treating autoimmune diseases is increasingly recognized by those involved in the research and clinical application of new biopharmaceuticals products. However, many aspect...

    Gecilmara Salviato Pileggi, Aline Dayana Clemencio, Thiago Malardo, Sonir R Antonini, Vania Luiza Deperon Bonato, Wendy Martin Rios and Celio L Silva

    BMC Biotechnology 2016 16:40

    Published on: 10 May 2016

  8. Research article

    Production of antigen-specific human IgGs by in vitro immunization

    We previously developed in vitro immunization based on a fusion protein containing the transcriptional transactivator (Tat) of human immunodeficiency virus and a double domain, called ZZ, derived from protein A o...

    A. Wijkhuisen, A. Savatier, N. Cordeiro and M. Léonetti

    BMC Biotechnology 2016 16:22

    Published on: 24 February 2016

  9. Research article

    Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen

    In an earlier study we developed a unique strategy allowing us to specifically eliminate antigen-specific murine B cells via their distinct B cell receptors using a new class of fusion proteins. In the present...

    Diana Klose, Ute Saunders, Stefan Barth, Rainer Fischer, Annett Marita Jacobi and Thomas Nachreiner

    BMC Biotechnology 2016 16:18

    Published on: 17 February 2016

  10. Methodology article

    HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

    The production of recombinant monoclonal antibodies in mammalian cell culture is of high priority in research and medical fields. A critical step in this process is the isolation of the antigen-binding domain ...

    Gaily Kivi, Kaupo Teesalu, Jüri Parik, Elen Kontkar, Mart Ustav Jr, Liis Noodla, Mart Ustav and Andres Männik

    BMC Biotechnology 2016 16:2

    Published on: 8 January 2016

  11. Research article

    Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

    Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary gluten and related prolamins. The only current therapeutic option is maintenance of a strict life-long gluten-free di...

    Valerie Stadlmann, Hanna Harant, Irina Korschineck, Marcela Hermann, Florian Forster and Albert Missbichler

    BMC Biotechnology 2015 15:109

    Published on: 1 December 2015

  12. Research article

    Defining the complementarities between antibodies and haptens to refine our understanding and aid the prediction of a successful binding interaction

    Low molecular weight haptens (<1000 Da) cannot be recognized by the immune system unless conjugated to larger carrier molecules. Antibodies to these exceptionally small antigens can still be generated with exq...

    Mohammed M. Al Qaraghuli, Soumya Palliyil, Gillian Broadbent, David C. Cullen, Keith A. Charlton and Andrew J. Porter

    BMC Biotechnology 2015 15:99

    Published on: 24 October 2015

  13. Research article

    Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain

    Botulism is a naturally occurring disease, mainly caused by the ingestion of food contaminated by the botulinum neurotoxins (BoNTs). Botulinum neurotoxins are the most lethal. They are classified among the six...

    Arnaud Avril, Sebastian Miethe, Michel R. Popoff, Christelle Mazuet, Siham Chahboun, Christine Rasetti-Escargueil, Dorothea Sesardic, Philippe Thullier, Michael Hust and Thibaut Pelat

    BMC Biotechnology 2015 15:86

    Published on: 17 September 2015

  14. Research article

    Construction of polyomavirus-derived pseudotype virus-like particles displaying a functionally active neutralizing antibody against hepatitis B virus surface antigen

    Virus-like particles (VLPs) can be efficiently produced by heterologous expression of viral structural proteins in yeast. Due to their repetitive structure, VLPs are extensively used for protein engineering an...

    Milda Pleckaityte, Corinna M. Bremer, Alma Gedvilaite, Indre Kucinskaite-Kodze, Dieter Glebe and Aurelija Zvirbliene

    BMC Biotechnology 2015 15:85

    Published on: 15 September 2015

  15. Research article

    An avian influenza A (H7N9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and Salmonella typhimurium flagellin

    A novel influenza virus, subtype H7N9, circulated through China in 2013–2014. Its higher rates of human infection in a wide range of locations within China and the associated increased likelihood of human-to-h...

    Li Song, Dan Xiong, Xilong Kang, Yun Yang, Jing Wang, Yaxin Guo, Hui Xu, Sujuan Chen, Daxin Peng, Zhiming Pan and Xinan Jiao

    BMC Biotechnology 2015 15:79

    Published on: 19 August 2015

  16. Research article

    Hybrid flagellin as a T cell independent vaccine scaffold

    To extend the potency of vaccines against infectious diseases, vaccines should be able to exploit multiple arms of the immune system. One component of the immune system that is under-used in vaccine design is ...

    Kaila M. Bennett, Ronald D. Gorham Jr, Veronica Gusti, Lien Trinh, Dimitrios Morikis and David D. Lo

    BMC Biotechnology 2015 15:71

    Published on: 12 August 2015

  17. Research article

    Generation in yeast of recombinant virus-like particles of porcine circovirus type 2 capsid protein and their use for a serologic assay and development of monoclonal antibodies

    Porcine circovirus type 2 (PCV2) is considered to be an important emerging pathogen associated with a number of different syndromes and diseases in pigs known as PCV2-associated diseases. It has been responsib...

    Juozas Nainys, Rita Lasickiene, Rasa Petraityte-Burneikiene, Jonas Dabrisius, Raimundas Lelesius, Vilimas Sereika, Aurelija Zvirbliene, Kestutis Sasnauskas and Alma Gedvilaite

    BMC Biotechnology 2014 14:100

    Published on: 9 December 2014

  18. Research article

    Lactococcus lactiscarrying the pValac DNA expression vector coding for IL-10 reduces inflammation in a murine model of experimental colitis

    Inflammatory bowel diseases (IBD) are intestinal disorders characterized by inflammation in the gastrointestinal tract. Interleukin-10 is one of the most important anti-inflammatory cytokines involved in the i...

    Meritxell Zurita-Turk, Silvina del Carmen, Ana CG Santos, Vanessa B Pereira, Denise C Cara, Sophie Y Leclercq, Alejandra dM de LeBlanc, Vasco Azevedo, Jean-Marc Chatel, Jean G LeBlanc and Anderson Miyoshi

    BMC Biotechnology 2014 14:73

    Published on: 9 August 2014

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