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Applied immunology

Section edited by Marko Radic

This section will consider studies on protein or cell engineering as they apply to the functions of the immune system and have applications in diagnostics, therapy and biomedical research.


  1. Content type: Methodology article

    Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early i...

    Authors: Rashmi Sharma, Fetweh H. Al-Saleem, Rama Devudu Puligedda, Amy Rattelle, David R. Lynch and Scott K. Dessain

    Citation: BMC Biotechnology 2018 18:41

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  2. Content type: Methodology article

    According to recent studies, the phospholipase A2 receptor 1 (PLA2R1) may be used as a biomarker to diagnose idiopathic membranous nephropathy (iMN). Moreover, the immune-dominant regions of PLA2R1 have been iden...

    Authors: Hua Li, Yonghua Xu, Weiming Yang and Xiaohong Huang

    Citation: BMC Biotechnology 2018 18:31

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  3. Content type: Research article

    α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent n...

    Authors: Zhoumin Li, Fang Wen, Zhonghui Li, Nan Zheng, Jindou Jiang and Danke Xu

    Citation: BMC Biotechnology 2017 17:72

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  4. Content type: Methodology article

    The T-cell receptor (TCR), located on the surface of T cells, is responsible for the recognition of the antigen-major histocompatibility complex, leading to the initiation of an inflammatory response. Analysin...

    Authors: Elisa Rosati, C Marie Dowds, Evaggelia Liaskou, Eva Kristine Klemsdal Henriksen, Tom H Karlsen and Andre Franke

    Citation: BMC Biotechnology 2017 17:61

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  5. Content type: Research article

    The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simu...

    Authors: Michael Puckette, Thomas Burrage, John G. Neilan and Max Rasmussen

    Citation: BMC Biotechnology 2017 17:52

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  6. Content type: Research article

    Preclinical research implementing fluorescence-based approaches is inevitable for drug discovery and technology. For example, a variety of contrast agents developed for biomedical imaging are usually evaluated...

    Authors: Felista L. Tansi, Ronny Rüger, Ansgar M. Kollmeier, Claudia Böhm, Roland E. Kontermann, Ulf K. Teichgraeber, Alfred Fahr and Ingrid Hilger

    Citation: BMC Biotechnology 2017 17:8

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  7. Content type: Methodology article

    There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied ...

    Authors: Laure-Hélène Ouisse, Laetitia Gautreau-Rolland, Marie-Claire Devilder, Michael Osborn, Melinda Moyon, Jonathan Visentin, Frank Halary, Marianne Bruggemann, Roland Buelow, Ignacio Anegon and Xavier Saulquin

    Citation: BMC Biotechnology 2017 17:3

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  8. Content type: Research article

    The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have de...

    Authors: Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono and Silvina Noemí Valdez

    Citation: BMC Biotechnology 2016 16:84

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  9. Content type: Methodology article

    Due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (DENV), chikungunya and zika virus, have developed into major public health challenges....

    Authors: Natalie Rose, Carlos Augusto Pinho-Nascimento, Alessia Ruggieri, Paola Favuzza, Marco Tamborrini, Hanna Roth, Marcia Terezinha Baroni de Moraes, Hugues Matile, Thomas Jänisch, Gerd Pluschke and Katharina Röltgen

    Citation: BMC Biotechnology 2016 16:83

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  10. Content type: Research article

    The valuable role of immunotherapy in treating autoimmune diseases is increasingly recognized by those involved in the research and clinical application of new biopharmaceuticals products. However, many aspect...

    Authors: Gecilmara Salviato Pileggi, Aline Dayana Clemencio, Thiago Malardo, Sonir R Antonini, Vania Luiza Deperon Bonato, Wendy Martin Rios and Celio L Silva

    Citation: BMC Biotechnology 2016 16:40

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  11. Content type: Research article

    We previously developed in vitro immunization based on a fusion protein containing the transcriptional transactivator (Tat) of human immunodeficiency virus and a double domain, called ZZ, derived from protein A o...

    Authors: A. Wijkhuisen, A. Savatier, N. Cordeiro and M. Léonetti

    Citation: BMC Biotechnology 2016 16:22

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  12. Content type: Research article

    In an earlier study we developed a unique strategy allowing us to specifically eliminate antigen-specific murine B cells via their distinct B cell receptors using a new class of fusion proteins. In the present...

    Authors: Diana Klose, Ute Saunders, Stefan Barth, Rainer Fischer, Annett Marita Jacobi and Thomas Nachreiner

    Citation: BMC Biotechnology 2016 16:18

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  13. Content type: Methodology article

    The production of recombinant monoclonal antibodies in mammalian cell culture is of high priority in research and medical fields. A critical step in this process is the isolation of the antigen-binding domain ...

    Authors: Gaily Kivi, Kaupo Teesalu, Jüri Parik, Elen Kontkar, Mart Ustav Jr, Liis Noodla, Mart Ustav and Andres Männik

    Citation: BMC Biotechnology 2016 16:2

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  14. Content type: Research article

    Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary gluten and related prolamins. The only current therapeutic option is maintenance of a strict life-long gluten-free di...

    Authors: Valerie Stadlmann, Hanna Harant, Irina Korschineck, Marcela Hermann, Florian Forster and Albert Missbichler

    Citation: BMC Biotechnology 2015 15:109

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  15. Content type: Research article

    Low molecular weight haptens (<1000 Da) cannot be recognized by the immune system unless conjugated to larger carrier molecules. Antibodies to these exceptionally small antigens can still be generated with exq...

    Authors: Mohammed M. Al Qaraghuli, Soumya Palliyil, Gillian Broadbent, David C. Cullen, Keith A. Charlton and Andrew J. Porter

    Citation: BMC Biotechnology 2015 15:99

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  16. Content type: Research article

    Botulism is a naturally occurring disease, mainly caused by the ingestion of food contaminated by the botulinum neurotoxins (BoNTs). Botulinum neurotoxins are the most lethal. They are classified among the six...

    Authors: Arnaud Avril, Sebastian Miethe, Michel R. Popoff, Christelle Mazuet, Siham Chahboun, Christine Rasetti-Escargueil, Dorothea Sesardic, Philippe Thullier, Michael Hust and Thibaut Pelat

    Citation: BMC Biotechnology 2015 15:86

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  17. Content type: Research article

    Virus-like particles (VLPs) can be efficiently produced by heterologous expression of viral structural proteins in yeast. Due to their repetitive structure, VLPs are extensively used for protein engineering an...

    Authors: Milda Pleckaityte, Corinna M. Bremer, Alma Gedvilaite, Indre Kucinskaite-Kodze, Dieter Glebe and Aurelija Zvirbliene

    Citation: BMC Biotechnology 2015 15:85

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  18. Content type: Research article

    Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit v...

    Authors: Afshin Hassani-Mehraban, Sjoerd Creutzburg, Luc van Heereveld and Richard Kormelink

    Citation: BMC Biotechnology 2015 15:80

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  19. Content type: Research article

    A novel influenza virus, subtype H7N9, circulated through China in 2013–2014. Its higher rates of human infection in a wide range of locations within China and the associated increased likelihood of human-to-h...

    Authors: Li Song, Dan Xiong, Xilong Kang, Yun Yang, Jing Wang, Yaxin Guo, Hui Xu, Sujuan Chen, Daxin Peng, Zhiming Pan and Xinan Jiao

    Citation: BMC Biotechnology 2015 15:79

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  20. Content type: Research article

    To extend the potency of vaccines against infectious diseases, vaccines should be able to exploit multiple arms of the immune system. One component of the immune system that is under-used in vaccine design is ...

    Authors: Kaila M. Bennett, Ronald D. Gorham Jr, Veronica Gusti, Lien Trinh, Dimitrios Morikis and David D. Lo

    Citation: BMC Biotechnology 2015 15:71

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  21. Content type: Research article

    Porcine circovirus type 2 (PCV2) is considered to be an important emerging pathogen associated with a number of different syndromes and diseases in pigs known as PCV2-associated diseases. It has been responsib...

    Authors: Juozas Nainys, Rita Lasickiene, Rasa Petraityte-Burneikiene, Jonas Dabrisius, Raimundas Lelesius, Vilimas Sereika, Aurelija Zvirbliene, Kestutis Sasnauskas and Alma Gedvilaite

    Citation: BMC Biotechnology 2014 14:100

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  22. Content type: Research article

    Prostate cancer is the most-diagnosed non-skin cancer among males in the US, and the second leading cause of cancer-related death. Current methods of treatment and diagnosis are not specific for the disease. T...

    Authors: Ryan M Williams, Cyrus J Hajiran, Sara Nayeem and Letha J Sooter

    Citation: BMC Biotechnology 2014 14:81

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  23. Content type: Research article

    Inflammatory bowel diseases (IBD) are intestinal disorders characterized by inflammation in the gastrointestinal tract. Interleukin-10 is one of the most important anti-inflammatory cytokines involved in the i...

    Authors: Meritxell Zurita-Turk, Silvina del Carmen, Ana CG Santos, Vanessa B Pereira, Denise C Cara, Sophie Y Leclercq, Alejandra dM de LeBlanc, Vasco Azevedo, Jean-Marc Chatel, Jean G LeBlanc and Anderson Miyoshi

    Citation: BMC Biotechnology 2014 14:73

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  24. Content type: Research article

    Tuberculosis is the leading cause of death due to bacterial infections worldwide, mainly caused by Mycobacterium tuberculosis. The antigen 85 complex comprises a set of major secreted proteins of M. tuberculosis,...

    Authors: Manon Fuchs, Susanne Kämpfer, Saskia Helmsing, Ralf Spallek, Wulf Oehlmann, Wiebke Prilop, Ronald Frank, Stefan Dübel, Mahavir Singh and Michael Hust

    Citation: BMC Biotechnology 2014 14:68

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  25. Content type: Research article

    The optimization of protein production is a complex and challenging problem in biotechnology. Different techniques for transcription, translation engineering and the optimization of cell culture conditions hav...

    Authors: Galina Gulis, Kelly Cristina Rodrigues Simi, Renata Rodrigues de Toledo, Andrea Queiroz Maranhao and Marcelo Macedo Brigido

    Citation: BMC Biotechnology 2014 14:26

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  26. Content type: Research article

    Vascular endothelial growth factor (VEGF) is a key angiogenic factors. It plays an important role in both physiologic and pathologic angiogenesis and increases permeability across the vessels. Using antibody p...

    Authors: Qun Wang, Jing Yang, Kun Tang, Longlong Luo, Liqiang Wang, Lei Tian, Yanming Jiang, Jiannan Feng, Yan Li, Beifen Shen, Ming Lv and Yifei Huang

    Citation: BMC Biotechnology 2014 14:17

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  27. Content type: Research article

    Consistent progress in the development of bacteriophage lambda display platform as an alternative to filamentous phage display system was achieved in the recent years. The lambda phage has been engineered to d...

    Authors: Emiliano Pavoni, Paola Vaccaro, Valeria D’Alessio, Rita De Santis and Olga Minenkova

    Citation: BMC Biotechnology 2013 13:79

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  28. Content type: Research article

    Tumor angiogenesis is critical for tumor growth, infiltration and metastasis. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor and targeting it is important in reducing angiogenesis. Bev...

    Authors: Weina Li, Yonggang Ran, Meng Li, Kuo Zhang, Xin Qin, Xiaochang Xue, Cun Zhang, Qiang Hao, Wei Zhang and Yingqi Zhang

    Citation: BMC Biotechnology 2013 13:77

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  29. Content type: Research article

    The demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by...

    Authors: Volker Jäger, Konrad Büssow, Andreas Wagner, Susanne Weber, Michael Hust, André Frenzel and Thomas Schirrmann

    Citation: BMC Biotechnology 2013 13:52

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  30. Content type: Research article

    A promising way in diagnostic and therapeutic applications is the development of peptide amphiphiles (PAs). Peptides with a palmitic acid alkylchain were designed and characterized to study the effect of the s...

    Authors: Christine Hüttl, Cornelia Hettrich, Reinhard Miller, Bernd-Reiner Paulke, Petra Henklein, Harshadrai Rawel and Frank F Bier

    Citation: BMC Biotechnology 2013 13:51

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  31. Content type: Research article

    The production process for the current influenza vaccine takes about 6 months and its antigenic composition must be modified annually. In the attempt towards developing influenza vaccine production that would ...

    Authors: Antonina Naskalska, Ewa Szolajska, Igor Andreev, Malgorzata Podsiadla and Jadwiga Chroboczek

    Citation: BMC Biotechnology 2013 13:50

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  32. Content type: Methodology article

    The ongoing global efforts to control influenza epidemics and pandemics require high-throughput technologies to detect, quantify, and functionally characterize viral isolates. The 2009 influenza pandemic as we...

    Authors: Sven N Hobbie, Karthik Viswanathan, Ido Bachelet, Udayanath Aich, Zachary Shriver, Vidya Subramanian, Rahul Raman and Ram Sasisekharan

    Citation: BMC Biotechnology 2013 13:34

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  33. Content type: Research article

    Sialoadhesin (Sn)-expressing monocytes/macrophages have been associated with several diseases like inflammatory and autoimmune disorders as well as viral infections, and they also appear to play a role in the ...

    Authors: Karen Ooms, Hanne Van Gorp, Tim Van Gaever, Hans J Nauwynck and Peter L Delputte

    Citation: BMC Biotechnology 2013 13:33

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  34. Content type: Research article

    The development of vectors for cell-specific gene delivery is a major goal of gene therapeutic strategies. Transferrin receptor (TfR) is an endocytic receptor and identified as tumor relative specific due to i...

    Authors: Qing Ye, Heyu Hu, Zhihua Wang, Tong Lu, Zhiquan Hu, Xing Zeng, Shu Zhang, Jing Liu, Ping Lei, Cong-Yi Wang, Zhangqun Ye and Guanxin Shen

    Citation: BMC Biotechnology 2012 12:91

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  35. Content type: Research article

    An antibody with cross-reactivity can create unexpected side effects or false diagnostic reports if used for clinical purposes. ERCC1 is being explored as a predictive diagnostic biomarker for cisplatin-based ...

    Authors: Donghui Ma, Dror Baruch, Youmin Shu, Kehu Yuan, Zairen Sun, Kaiyan Ma, Toan Hoang, Wei Fu, Li Min, Zhu-Sheng Lan, Fangxun Wang, Lori Mull and Wei-Wu He

    Citation: BMC Biotechnology 2012 12:88

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  36. Content type: Methodology article

    Production of human monoclonal antibodies that exhibit broadly neutralizing activity is needed for preventing HIV-1 infection, however only a few such antibodies have been generated till date. Isolation of ant...

    Authors: Rajesh Kumar, Raiees Andrabi, Ashutosh Tiwari, Somi Sankaran Prakash, Naveet Wig, Durgashree Dutta, Anurag Sankhyan, Lubina Khan, Subrata Sinha and Kalpana Luthra

    Citation: BMC Biotechnology 2012 12:87

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  37. Content type: Research article

    Arenavirus matrix protein Z plays an important role in virus budding and is able to generate enveloped virus-like-particles (VLPs) in absence of any other viral proteins. In these VLPs, Z protein is associated...

    Authors: Cristina S Borio, Marcos F Bilen, Marcelo H Argüelles, Sandra E Goñi, Javier A Iserte, Graciela Glikmann and Mario E Lozano

    Citation: BMC Biotechnology 2012 12:80

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  38. Content type: Research article

    The recent H1N1 influenza pandemic illustrated the shortcomings of the vaccine manufacturing process. The A/California/07/2009 H1N1 pandemic influenza vaccine or A(H1N1)pdm09 was available late and in short su...

    Authors: Elena Feshchenko, David G Rhodes, Rachael Felberbaum, Clifton McPherson, Joseph A Rininger, Penny Post and Manon MJ Cox

    Citation: BMC Biotechnology 2012 12:77

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  39. Content type: Research article

    Interleukin-1 receptor antagonist, a cytokine that is highly therapeutic to rheumatoid arthritis and several other inflammatory diseases, exhibits rapid blood clearance and poor retention time on the target in...

    Authors: Mengyuan Liu, Yi Huang, Lei Hu, Guoping Liu, Xueping Hu, Dongxu Liu and Xiaosong Yang

    Citation: BMC Biotechnology 2012 12:68

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  40. Content type: Methodology article

    Cell panning of phage-displayed antibody library is a powerful tool for the development of therapeutic and imaging agents since disease-related cell surface proteins in native complex conformation can be direc...

    Authors: Hyerim Yoon, Jin Myung Song, Chun Jeih Ryu, Yeon-Gu Kim, Eun Kyo Lee, Sunghyun Kang and Sang Jick Kim

    Citation: BMC Biotechnology 2012 12:62

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  41. Content type: Research article

    Costimulation of T cells via costimulatory molecules such as B7 is important for eliciting cell-mediated antitumor immunity. Presenting costimulation molecules by immobilizing recombinant B7 on the surface of ...

    Authors: Ming-Chuan Li, Qian-Qian Liu, Xiao-Yun Lu, Ya-Li Zhang and Lei-Lei Wang

    Citation: BMC Biotechnology 2012 12:43

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