Fig. 5
From: A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility
Verification of enteric glial cell-specific Mpz-EPOR-KO in mice. A Characterization of mouse enteric glial cells (EGCs). The identity and purity of SCs were confirmed using IF staining of GFAP, p75NTR, and S100 under a fluorescent microscope (ZEISS Apotome 2). The purity of the cultured EGCs (99%) was analyzed by double positive staining of DAPI with GFAP/p75NTR/S100 markers from 3 independent experiments. Each image represents 3 images from 3 independent experiments. Scale bar: 100 μm, n = 3. B PCR genotyping with DNA isolated from EGCs (passage zero, EGC-P0; passage one, EGC-P1) revealed no deletion of EPOR in MpzCre-EPORflox/flox mice. EPOR bands of wild-type (control; 390 bp) and flox/flox (428 bp) tail samples of mice are depicted (n = 3). C PCR genotyping with DNA isolated from the segments of the intestine (D, duodenum; J, jejunum; I, ileum) revealed no deletion of EPOR in MpzCre-EPORflox/flox mice (n = 3)