Fig. 4
From: A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility
Verification of vagus nerve Schwann cell-specific Mpz-EPOR-KO in mice. A Characterization of mouse vagus nerve derived Schwann cells (VNSCs). The identity and purity of SCs were confirmed using IF staining of S100, p75NTR, and Mpz under a fluorescent microscope (ZEISS Apotome 2). The purity of the cultured SCs (99%) was analyzed by double positive staining of DAPI with S100/p75NTR/Mpz markers from 3 independent experiments. Each image represents 3 images from 3 independent experiments. Scale bar: 50 μm, n = 3. B PCR genotyping with DNA isolated from the vagus nerve and VNSCs (passage zero, SC-P0; passage one, SC-P1) revealed the presence of the 220-bp PCR product, resulting from MpzCre mediated recombination in the Schwann cell of MpzCre-EPORflox/flox mice and not in wild-type (control; 390 bp) and flox/flox (428 bp) mice (n = 3)