Table 4 CCD design matrix with coded values along with the experimental and predicted rhamnolipid production
Run no | Independent variables | Rhamnolipid (g/kg of substrate) | ||||
|---|---|---|---|---|---|---|
A: Temperature (°C) | B: Inoculum size (mL) | C: Humidity (%) | D: Glycerol (%) | Experimental | Predicted | |
1 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 19.67 | 19.61 |
2 | 38.00 (+ α) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 14.53 | 14.98 |
3 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 20.12 | 19.61 |
4 | 36.00 (1) | 1.00 ( − 1) | 80.00 (1) | 6.00 (1) | 16.1 | 15.89 |
5 | 36.00 (1) | 1.60 (1) | 80.00 (1) | 4.00 ( − 1) | 17.83 | 17.43 |
6 | 32.00 ( − 1) | 1.60 (1) | 80.00 (1) | 4.00 ( − 1) | 14.24 | 14.66 |
7 | 32.00 ( − 1) | 1.00 ( − 1) | 80.00 (1) | 4.00 ( − 1) | 16.42 | 16.13 |
8 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 19.11 | 19.61 |
9 | 32.00 ( − 1) | 1.00 ( − 1) | 70.00 ( − 1) | 6.00 (1) | 13.97 | 14.38 |
10 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 19.56 | 19.61 |
11 | 32.00 ( − 1) | 1.00 ( − 1) | 80.00 (1) | 6.00 (1) | 15.02 | 15.02 |
12 | 36.00 (1) | 1.60 (1) | 70.00 ( − 1) | 6.00 (1) | 16.25 | 16.55 |
13 | 32.00 ( − 1) | 1.60 (1) | 80.00 (1) | 6.00 (1) | 17.43 | 17.62 |
14 | 32.00 ( − 1) | 1.60 (1) | 70.00 ( − 1) | 4.00 ( − 1) | 14.42 | 14.64 |
15 | 32.00 ( − 1) | 1.60 (1) | 70.00 ( − 1) | 6.00 (1) | 18.13 | 17.78 |
16 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 7.00 (+ α) | 15.12 | 15.13 |
17 | 34.00 (0) | 1.30 (0) | 65.00 ( − α) | 5.00 (0) | 16.78 | 17 |
18 | 30.00 ( − α) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 13.68 | 13.4 |
19 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 19.53 | 19.67 |
20 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 5.00 (0) | 19.71 | 19.67 |
21 | 36.00 (1) | 1.00 ( − 1) | 70.00 ( − 1) | 6.00 (1) | 13.81 | 13.19 |
22 | 36.00 (1) | 1.60 (1) | 70.00 ( − 1) | 4.00 ( − 1) | 15.55 | 15.04 |
23 | 34.00 (0) | 1.30 (0) | 75.00 (0) | 3.00 ( − α) | 14.88 | 15.4 |
24 | 36.00 (1) | 1.00 ( − 1) | 70.00 ( − 1) | 4.00 ( − 1) | 16.24 | 16.06 |
25 | 34.00 (0) | 1.90 (+ α) | 75.00 (0) | 5.00 (0) | 17.89 | 17.67 |
26 | 36.00 (1) | 1.60 (1) | 80.00 (1) | 6.00 (1) | 18.42 | 18.46 |
27 | 34.00 (0) | 0.70 ( − α) | 75.00 (0) | 5.00 (0) | 15.4 | 15.31 |
28 | 34.00 (0) | 1.30 (0) | 85.00 (+ α) | 5.00 (0) | 19.76 | 19.71 |
29 | 36.00 (1) | 1.00 ( − 1) | 80.00 (1) | 4.00 ( − 1) | 18.79 | 18.94 |
30 | 32.00 ( − 1) | 1.00 ( − 1) | 70.00 ( − 1) | 4.00 ( − 1) | 15.55 | 15.31 |