Fig. 4

Cell morphology and gene expression in LPS-stimulated cells. A) LPS-stimulated RAW 264.7 cells at 400X magnification 24 h after addition and aspiration of P/GI to a single well various distances away (A2, A3, and A4) or time-matched LPS-treated but P/GI-unexposed controls (A1). B) RT-PCR data measuring expression of Il6 transcripts in RAW 264.7 cells cultured at various distances from P/GI residue at 4 h, 8 h, and 24 h after LPS exposure (B1, B2, and B3, respectively). Transcripts are normalized so that the average expression in P/GI-unexposed controls equals 1