Fig. 7

Inhibition effects of MS473 on the production of cytokines from human peripheral blood mononuclear cells (PBMCs) stimulated with TSST-1. To assess the inhibition effect of MS473 on the TSST-1-induced cytokine secretion, fresh human PBMCs (~ 10⁶ cells/ml) were incubated simultaneously with the TSST-1 protein (2.1 nM) and MS473 (3 µM) for 24 h at 37 °C, 5% CO2. The cells treated with PBS or the TSST-1 protein (2.1 nM) and PBS served as the controls. The concentration of human interleukin 2 (IL)-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17 A, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, G-CSF, and transforming growth factor (TGF)-β in the culture supernatant of PBMCs (stimulated and unstimulated) were calculated using the standard curve provided in a Multi-Analyte ELISArray Kit (Qiagen). The concentration of cytokines (pg/ml) is represented on a logarithmic scale. The concentrations of IL-4, IL-5, IL-12, and IL-13 in the culture supernatant of PBMCs treated with TSST1 and MS473 (or PBS) were less than the limit of detection (LOD). Moreover, the concentrations of IL-2, IL-17 A, and G-CSF in the culture supernatant of PBMCs treated with PBS were less than the LOD. The samples were run in triplicate, and the results are expressed as mean ± SD. *P < 0.05, **P = 0.01, ***P < 0.01