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Fig. 5 | BMC Biotechnology

Fig. 5

From: Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion

Fig. 5

Reactivity of MS473 to TSST-1 peptides involved in the TCR binding. The MS473 scFv reacted significantly with the peptides T1 and T2, which are located in the region of TSST-1 that interacts with the human TCR Vβ-chain. There was no significant reaction between the MS473 scFv and peptides M1 and M2, both of which are located within the region of TSST-1 that interacts with the MHC class II. The RA16 scFv, a control scFv against IL-6, showed significant reactivity with the IL-6 peptide but not with the TSST-1 peptide. The samples were run in triplicate, and the results are expressed as mean ± SEM of at least three individual experiments. Statistical comparisons were carried out with Student’s t-test. *P < 0.01

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