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Fig. 4 | BMC Biotechnology

Fig. 4

From: Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion

Fig. 4

The binding affinity and specificity of MS473 to TSST-1. (A) The binding affinity of MS473 and RA16 (an scFv specific to IL-6) TSST-1 (1 and 2 µg/ml) was measured by non-competitive ELISA. (B) The specific binding of MS473 to TSST-1 was evaluated by ELISA. The wells coated with adiponectin, α-hemolysin, bovine serum albumin (BSA), skimmed milk powder, and TSST-1 were incubated with MS473, followed by mouse anti-human scFv polyclonal antibody and goat anti-mouse IgG-horseradish peroxidase (HRP)-conjugated antibody. The MS473 scFv exhibited significant binding to TSST-1 compared to the control proteins. The samples were run in triplicate, and the results are expressed as mean ± SEM of at least three individual experiments. Statistical comparisons were carried out with the one-way analysis of variance (ANOVA). *P < 0.05

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