Table 1 Effects of protease inhibitors, reducing reagent, and surfactants on the keratinase activity
Chemicals | Concentration | Relative activity (%) |
|---|---|---|
PMSF | 5Â mM | ND* |
EDTA | 5 mM | 6.17 ± 0.52 |
β-ME | 5 mM | 97.53 ± 4.31 |
SDS | 1% | 101.91 ± 2.63c |
Tween 20 | 5% | 200.26 ± 7.35a |
Tween 60 | 5% | 163.62 ± 8.23b |
Tween 80 | 5% | 108.98 ± 2.26c |
Triton X-100 | 5% | 107.51 ± 7.13c |