Fig. 1

Primary culture and characterization of chicken embryonic brain cells. A Morphology of primary cultured chicken brain cells. Scale bar, 200 μm. B RT-PCR measurement of PAX6 and SYP expression in primary cultured brain cells. DF1 fibroblast cells were used as a negative control. DW, distilled water. Uncropped gel images are provided in Additional file 2: Fig. S1. C Primary brain cells cultured for 2 weeks were immunostained with anti-NeuN antibody. DF1 fibroblast cells were used as a negative control. Scale bar, 50 μm