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Table 1 Summary of TAG content and expression/suppression of transgenes in leaves of transgenic energycane

From: Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass

Line

TAG (% of DW)

Expression/suppression of transgenes in leaves

ZmDGAT1-2

SiCysOLE1

TGD1 (%)

SDP1 (%)

CP 82-1592

0.05 ± 0.02a

0.00 ± 0.00a

0.00 ± 0.00a

100 ± 19ab

100 ± 9abcd

P27

0.11 ± 0.05a

0.08 ± 0.01abc

0.02 ± 0.00cd

39 ± 6ef

152 ± 37e

P24

0.12 ± 0.07a

0.00 ± 0.01a

0.01 ± 0.00a

51 ± 15de

119 ± 12abcd

P19

0.12 ± 0.01a

0.06 ± 0.01ab

0.00 ± 0.00ab

106 ± 12a

120 ± 13abcde

P26

0.13 ± 0.00a

0.14 ± 0.01bcd

0.02 ± 0.00cd

33 ± 6ef

130 ± 32cde

P25

0.18 ± 0.02a

0.16 ± 0.01cd

0.00 ± 0.00a

23 ± 10f

149 ± 11e

P28

0.19 ± 0.05a

0.15 ± 0.02bcd

0.03 ± 0.00cd

18 ± 2f

121 ± 31bcde

P20

0.21 ± 0.01a

0.20 ± 0.09cd

0.03 ± 0.01cd

100 ± 16ab

139 ± 7abcde

P6

0.21 ± 0.05a

0.31 ± 0.04f

0.02 ± 0.00cd

113 ± 3a

169 ± 11de

P23

0.30 ± 0.18a

0.30 ± 0.02ef

0.00 ± 0.00a

98 ± 17ab

174 ± 11e

P7

0.36 ± 0.02a

0.34 ± 0.01f

0.07 ± 0.00e

104 ± 17a

105 ± 8abcd

P5

0.65 ± 0.09ab

0.55 ± 0.07g

0.26 ± 0.01f

65 ± 7cd

70 ± 12af

P8

0.90 ± 0.06ab

1.63 ± 0.06k

0.01 ± 0.00bc

51 ± 15de

95 ± 16abc

P16

1.52 ± 0.45bc

1.44 ± 0.08h

0.01 ± 0.00ab

70 ± 1bcd

81 ± 16abf

  1. TAG and gene expression values shown for each line represent leaf extracts from three biological replicates. Values are expressed as means ± SDs. Values within one column with different letters are significantly different at p ≤ 0.05 according to a one-way ANOVA test and the Duncan’s Multiple Range Test (MRT), administered post hoc. The expression of transgenes is shown relative to GAPDH. Suppression of RNAi target genes is shown as a percentage of the non-modified CP 82-1592 control